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독도서식 식물근권에서 분리한 포자형성세균과 질소고정세균의 군집구조 분석
전선애,성혜리,박유미,박재홍,김사열,Jeon, Seon-Ae,Sung, Hye-Ri,Park, Yu-Mi,Pak, Jae-Hong,Ghim, Sa-Youl 한국미생물·생명공학회 2009 한국미생물·생명공학회지 Vol.24 No.3
Bacteria were isolated from roots of plants belonging to family Solanaceae and Gramineae, inhabited in Dokdo island. Fifty six endospore-forming bacteria grown on tryptic soy broth (TSB) agar medium and 23 nitrogen-fixing bacteria (NFB) grown on nitrogen free agar medium were isolated, respectively. The isolates were partially identified by analyzing the 16S rDNA and categorized into phylogenetic groups. The 16S rDNA sequences of each identified isolates were compared with sequences of each type strains to analyze phylogenetic relationship by phylogenetic tree. As a result, endospore-forming bacteria and nitrogen-fixing bacteria were classified into 4 and 6 lineage groups, respectively. Among these isolated, 18 were presumed to be novel species candidates based on the similarity (lower than 98%) analysis of the l6S rDNA sequences.
독도 주변의 해수에서 분리한 세균의 다양성과 군집구조 분석
김사열 ( Sa Youl Ghim ),성혜리 ( Hye Ri Sung ) 한국미생물생명공학회 2010 한국미생물·생명공학회지 Vol.38 No.3
One hundred sixty three strains showing different colony morphological characteristics on different concentration of marine agar (MA) plates were isolated from ambient seawater near Dokdo island. Bacterial diversity and distributions were studied by phylogenetic analysis of the partial 16S rRNA gene sequences. One hundred sixty three strains were partially sequenced and analyzed phylogenetically. They were composed of 5 phyla, of which gamma-proteobacteria (58%), alpha-proteobacteria (20%), bacteriodetes (16%) were predominant. They were affiliated with 90 species. The 16S rRNA sequence similarity of the isolates was in 93.3 to 100 % range to reported sequence data. Thirty six isolates of among them were assumed to be novel species candidates based on similarity analysis of the 16S rRNA gene sequences. Overall, Proteobacteria and Bacteriodetes of the Dokdo coastal sea water showed a high diversity.
유전자조작, 균주분리 독도에 서식하는 가지과식물로부터 분리된 근권세균의 특성
함미선 ( Mi Seon Ham ),박유미 ( Yu Mi Park ),성혜리 ( Hye Ri Sung ),류충민 ( Choong Min Ryu ),박승환 ( Seung Hwan Park ),김사열 ( Sa Youl Ghim ),( Marilyn Sumayo ) 한국미생물생명공학회 2009 한국미생물·생명공학회지 Vol.37 No.2
To characterize plant root-associated bacteria in wild plant family Solanaceae, Solanum nigrum L. plants were collected in Dokdo island. Forty four strains of nitrogen-fixing or spore-forming bacteria were isolated from rhizosphere of Solanum nigrum L. plants. Among these, 19 strains were able to produce auxin. Thirteen strains of these produced siderophore as determined by color reaction on CAS-blue plate, 8 strains were able to solubilize phosphate. The 16S rDNA genes of the isolated bacteria were amplified and sequenced. Model plants, pepper and tobacco, were established in order to evaluate the bacterial capacities eliciting growth promotion and induced systemic resistance. The plants treated with strain KUDC1009 were more resistant and capable of growth-promotion than control plants when challenged by either Xanthomonas axonopodis pv. vesicatoria or Erwinia carotovora sub. carotovora strain SCC1. Rhizobacteria isolated from Dokdo island can promote growth of wild type Solanum nigrum L. under much environmental stresses.