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유전자조작, 균주분리 독도에 서식하는 가지과식물로부터 분리된 근권세균의 특성
함미선 ( Mi Seon Ham ),박유미 ( Yu Mi Park ),성혜리 ( Hye Ri Sung ),류충민 ( Choong Min Ryu ),박승환 ( Seung Hwan Park ),김사열 ( Sa Youl Ghim ),( Marilyn Sumayo ) 한국미생물생명공학회 2009 한국미생물·생명공학회지 Vol.37 No.2
To characterize plant root-associated bacteria in wild plant family Solanaceae, Solanum nigrum L. plants were collected in Dokdo island. Forty four strains of nitrogen-fixing or spore-forming bacteria were isolated from rhizosphere of Solanum nigrum L. plants. Among these, 19 strains were able to produce auxin. Thirteen strains of these produced siderophore as determined by color reaction on CAS-blue plate, 8 strains were able to solubilize phosphate. The 16S rDNA genes of the isolated bacteria were amplified and sequenced. Model plants, pepper and tobacco, were established in order to evaluate the bacterial capacities eliciting growth promotion and induced systemic resistance. The plants treated with strain KUDC1009 were more resistant and capable of growth-promotion than control plants when challenged by either Xanthomonas axonopodis pv. vesicatoria or Erwinia carotovora sub. carotovora strain SCC1. Rhizobacteria isolated from Dokdo island can promote growth of wild type Solanum nigrum L. under much environmental stresses.