http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 석윤 (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
삼차원 PLGA스폰지를 이용한 연골세포의 생체이식: 이식 전의 생체 외 배양은 필요한가?
석윤,박재구,이종원,조해석,·문덕수,정연희,주영민,한동근 대한성형외과학회 2002 Archives of Plastic Surgery Vol.29 No.3
For tissue-engineered neocartilage formation in vivo, most studies have cultured chondrocytes within a biodegradable polymer in vitro before implantation of cell-polymer complex into an animal. The present study was performed to investigate the necessity of in vitro culture (preconditioning). Cell-polymer complex was made of chondrocytes obtained from a rabbit ear and a 85:15 poly(DL-lactic-co- glycolic acid) (PLGA) sponge. The complex was implanted into a nude mouse either after or without in vitro preconditioning. For control groups, PLGA sponge without chondrocytes was used. One, 2, and 4 weeks after the implantation, each group was examined by measurement of weight and volume of sponges as well as histologic study with Safranin-O staining. The control groups showed loss of weight as time passed. The non-preconditioned group, on the other hand, showed weight loss for the first week, but increased in weight afterwards. The preconditioned group also had weight loss in the first week after the implantation with no noticeable weight changes thereafter. Neither weight nor volume of PLGA sponges in preconditioned group was significantly different from those in non-preconditioned group until the 2nd week. In the 4th week, volume of sponges in non-preconditioned group was significantly larger than that in preconditioned group. On histological observation, chondrocytes seeded into a PLGA sponges proliferated and differentiated into cartilage tissues both in preconditioned and non-preconditioned groups, but non-preconditioned group formed cartilage tissue more extensively than the preconditioned group. Based on the above results, it is suggested that new cartilage tissues can be formed successfully following the implantation of cell- polymer complex into a living body without any prior conditioning in a separate culture system.
-
-
권호,윤인모,석윤,조현미,이종원,안상태 대한성형외과학회 2003 Archives of Plastic Surgery Vol.30 No.5
Alginate gel is widely used as a scaffold in tissue engineering. Alginate solution has anionic properties, and calcium or magnesium cation has been used to crosslink alginate into a gel form. Chitosan not only has cationic properties but also is known to promote wound healing. Although there are some studies of chitosan- alginate gel use in drug delivery, reports of its application as a scaffold in tissue engineering are rare. The purpose of this study is to make chitosan-alginate gel and to investigate its biocompatibility as a scaffold for preadipocyte culture.1, 2, 4, 6% chitosan solutions were mixed with 2% alginate solution to make various concentrations of chitosan-alginate gels. All of the gel which were made have been measured by Viscometer. Preadipocytes obtained from human breast fat tissue were seeded into each chitosan-alginate gel, and cell viability was measured by XTT colorimetric assay on the 2th, 4th, and 7th day of preadipocyte culture. The results of analysis were as follows. Each viscosity of 4% and 6% chitosan-alginate gels is similar to that of the calcium-alginate gel and 4% and 6% chitosan-alginate gels shows significantly higher cell viability than the calcium-alginate gel(p<0.05).In conclusion, chitosan-alginate gel is thought to be an appropriate scaffold for preadipocyte culture in tissue engineering.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
