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- 저자 : 석애은 (검색결과 4 건)
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Screening of Sera from Patients with Pancreatitis by an Apoptosis Assay of Skin-derived Cells
석애은,손병관,이지영,정광현,이유림,김두진,차병헌,강희규 대한소화기학회 2019 대한소화기학회지 Vol.74 No.4
Background/Aims: An excessive inflammatory response is typical in acute pancreatitis and a significant cause of early mortality in severe acute pancreatitis. This is believed to be caused by inflammatory molecules or upregulated cytokine levels in the serum of patients. The aim of this study was to identify the serum-mediated apoptosis-inducing effects in acute pancreatitis patients. Methods: A skin tissue-derived cell line, BJ, was treated for 24 hours with the sera of 22 healthy volunteers (control) and 71 acute pancreatitis patients (22 with gallstone pancreatitis, 16 with alcoholic pancreatitis, and 11 with pancreatitis with other causes) collected at the time of hospital admission (active) and discharge (resolved). Apoptosis was analyzed by flow cytometry. Results: The average percentage of living cells, early apoptotic cells, and late apoptotic cells ranged from 78.8% to 85.0%, 5.5% to 7.3%, and 7.7% to 13.1%, respectively. The number of live cells increased significantly using the serum from the resolved state of gallstone-induced pancreatitis. In addition, the number of early apoptotic cells increased significantly using the serum from the resolved state of pancreatitis with other causes. The number of late apoptotic cells decreased significantly with the serum from the resolved state compared to the active state of gallstone- and alcohol-induced pancreatitis. Conclusions: Serum samples from patients with pancreatitis induced a change in the apoptosis profiles of skin-derived cells. These results indicate changes in the serum components in patients with acute pancreatitis.
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질량분석기를 활용한 막 단백질 비교분석: High-speed Centrifuge법과 Reagent-based법
이지영,석애은,박아름,문소라,강희규 대한임상검사과학회 2019 대한임상검사과학회지(KJCLS) Vol.51 No.1
Membrane proteins are involved in many common diseases, including heart disease and cancer. In various disease states, such as cancer, abnormal signaling pathways that are related to the membrane proteins cause the cells to divide out of control and the expression of membrane proteins can be altered. Membrane proteins have the hydrophobic environment of a lipid bilayer, which makes an analysis of the membrane proteins notoriously difficult. Therefore, this study evaluated the efficacy of two different methods for optimal membrane protein extraction. High-speed centrifuge and reagent-based method with a -/+ filter aided sample preparation (FASP) were compared. As a result, the high-speed centrifuge method is quite effective in analyzing the mitochondrial inner membranes, while the reagent-based method is useful for endoplasmic reticulum membrane analysis. In addition, the function of the membrane proteins extracted from the two methods were analyzed using GeneGo software. GO processes showed that the endoplasmic reticulum-related responses had higher significance in the reagent-based method. An analysis of the process networks showed that one cluster in the high-speed centrifuge method and four clusters in the reagent-based method were visualized. In conclusion, the two methods are useful for the analysis of different subcellular membrane proteins, and are expected to assist in selecting the membrane protein extraction method by considering the target subcellular membrane proteins for study. 막 단백질은 심장질환, 암과 같은 우리 주변에서 흔히 발생하는 질병에 관련되어 있다. 이러한 암과 같은 특정한 질환 상태에서, 막 단백질과 관련된 신호 전달의 비정상은 세포분열을 통제하지 못하고 증가시킬 수 있으며 막 단백질의 발현에 변화가 생긴다. 막 단백질은 지질 이중층으로 이루어진 소수성 환경을 가지고 있어 불안정하기 때문에 막 단백질을 추출해서 연구를 수행하는데 어려움이 있다. 이번 연구에서는 최적화된 막 단백질추출법을 확인하고자 서로 다른 두 가지 추출법의 효율성을 평가하였다. 두 가지 방법으로, high-speed centrifuge법과 reagent법이비교되었다. 비교 분석결과, 미토콘드리아 내막 단백질 분석에는 high-speed centrifuge법이 효율적이고, 소포체막 단백질 분석에는 reagent법이 유용함을 확인하였다. 게다가유전자 온톨로지 소프트웨어를 이용해서 추출된 막 단백질의기능분석을 진행하였을 때, 유전자 온톨로지는 reagent법에서소포체 막 단백질에 연관된 반응이 활성화 되는 것을 확인할 수있었다. 프로세스 네트워크 분석에서, high-speed centrifuge 법에서는 하나의 클러스터를 형성화는 반면, reagent법에서는네 개의 클러스터를 형성하는 것을 시각화하여 확인하였다. 결론적으로, 두 가지 분석법은 서로 다른 하위 막 단백질의 분석에유용함을 확인할 수 있었다. 이러한 결과를 토대로, 막 단백질을분석할 때, 표적의 세부 막 단백질을 고려하여 방법론을 선택하는데 도움을 줄 것으로 기대된다.
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뇌파(EEG)를 통한 콘텐츠 환경에 따른 집중도 차이 분석
김우섭(Wooseop Kim),정덕영(Dukyoung Jung),신영석(Youngseok Shin),구원회(Wonhoe Gu),석애은(Ae-eun Seok),조현영(Hyunyoung Cho),최민라(Minra Choi) 대한전자공학회 2019 대한전자공학회 학술대회 Vol.2019 No.11
In 2018, the number of adults aged 65 and older already approached 14% of total population. It is grown rapidly by year. the increase is principally due to the maturing of baby boomer born in Korea between 1955 and 1963. In 2020, the first baby boomer will turn 65, and by 2028 the entire baby boom generation will be 65 or older. They describe themselves as thriving and healthy and consider aging well as a lifestyle and have an enormous economic power and hope to engaging a variety of cultural and leisure activities. Most of them are often used to enjoy internet contents through a smartphone or PC. But it is not easy to access because of contents’ user interface or environment that was focused on eco generation. So, we study that how does web environment affect attention of elderly people. Using the electroencephalography, we measured and analyzed averaged band power and calculated beta/alpha, theta/beta ratios. As a result, generally delta, theta band power and theta/beta ratio are higher in normal environment. On the contrary beta power, beta/alpha ratio are higher in customized environment that is customized reflecting cognitive function of elderly people. These results propose that the attention of elderly people varies among web environments.
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