五味子抽出物이 Alloxan 負荷家鬼의 血淸成分에 미치는 影響

서화중(Sheo Hwa Joong),이명열(Lee Myung YuI),황경숙(Hwang Gyoung Sook) 한국식품영양과학회 1987 한국식품영양과학회지 Vol.16 No.4

The effect of Schizandrae fructus extract (S. F. E.) on experimentallly alloxan-induced diabetes in rabbits and the acute toxicity on mice were studied;<br/> LD_(50) of the extract was 2l.50g/㎏ by intraperitoneal administration in mice.<br/> S. F. E. showed more rapid recovering effect than the control group; all samples showed excellent effect of lowering the hyperglycemia, that is, the blood glucose level was significantly decreased by 800㎎/㎏ in 2 days and by 200㎎/㎏ in 6 days.<br/> SGPT activity was lowered promptly after 4 days in 800㎎/㎏.<br/> Total cholesterol level was not shown significant lowering effect by 200㎎/㎏, but rapid by 800㎎/㎏, in 6 days; blood urea nitrogen level was decreased gradually in 800㎎/㎏ after 10 days and 14 days in 200㎎/㎏.<br/> In histological studies of pancreas, the sample groups exhibited less karyorrhexis, vacuolar and vesicular change, more stable in contents of β-cells than the control group.

한국산 고사리의 독성조사에 관한 연구

서화중(Sheo Hwa Joong),이명렬(Lee Myung Yul) 한국식품영양과학회 1989 한국식품영양과학회지 Vol.18 No.3

고사리의 ethanol extract를 독성실험한 결과는 mouse에 시료 10g/㎏까지는 급성독(LD_(50))을 나타내지 않았다.<br/> Rat에 매일 시료40㎎/㎏, 400㎎/㎏, 1200㎎/㎏을 경구투여한 처음 7일간에는 체중, 해부소견, 혈액의 생화학적 검사에서 별이상이 없었다.<br/> 14일째 전시료투여군의 체중증가율은 대조군보다 평균 약 7% 감소를 보이고 혈청의 생화학적 측정치가 대부분 약간 증가하였다.<br/> 21일째 400㎎/㎏군과 1200㎎/㎏군의 체중증가율이 대조군보다 6.7~5.7% 감소되고 1200㎎/㎏군 1필이 황색간증세를 보였다.<br/> 모든 시험군 GPT 및 GOT는 각각 88~102μ와 164~200μ로 증가하고 alkaline phosphatase도 상승하여 중독의 징후를 보였다.<br/> 28일째 400㎎/㎏군과 1200㎎/㎏군의 체중증가율이 대조군보다 감소되고 이들군에서 각각 1~2필이 황색간을 보였다.<br/> 1200㎎/㎏군 2필이 소장염을 보였다. 모든 시험군은 GPT 92~116μ와 GOT 56~215μ 및 alkaline phosphatase 57~70μ로 증가 되었다. The results of toxicity test using mice and rats for ethanol extract of Bracken Fern collected in Kwang Ju area were shown as follows ;<br/> Up to the dose of 10g per ㎏ of mice administered intraperitoneally there was no lethal toxicity so that it was impossible to calculate the median lethal dose (LD_(50)).<br/> For the first 7 days experiment all rats administered frond extract grouping in 40㎎, 400㎎, and 1200㎎ per ㎏ of rat as the daily oral doses did not show any characterized sign in the weight gain rate, anatomical findings, and biochemical studies. For 3 weeks following the first week the weight gain rates of all test group were reduced to 4.2~7% below the weight gain rate of control.<br/> In this period serum GPT, GOT, and Alkaline phosphatase value were increased significantly indicating the symptoms of Bracken Fern poisonings. The pathological findings of all test groups for 28 days showed acute and chronic intestinal lesion and liver damage with steatosis especially in 1200g/㎏ rat groups. In this experiment the Bracken poisonings appeared slowly in rats of 400㎎/㎏ and 1200㎎/㎏ for two weeks and in rats of 40㎎/㎏ for 3 weeks, showing the symptoms of lowering of weight gain rate, subacute hepatitis, hepatic steatosis and enteritis in 28 days experiment.

영산강에 서식하는 담수어의 중금속 함유량에 관하여

서화중(Hwa-Joong Sheo),홍석순(Seog-Soon Hong),김충모(Choong-Mo Kim) 한국식품영양과학회 1991 한국식품영양과학회지 Vol.20 No.6

1990년 영산강의 오염예상지역 송정리와 오염가능지역 동강 및 비오염예상지역 나주호에 서식하는 담수어의 중금속 함유량 측정 결과는 다음과 같다.<br/> Hg 함유량은 평균 0.022~0.139ppm분포이고, 동강의 메기근육에서 가장 높고 동지역의 잉어 아가미가 가장 낮았다. 어종별 수은 함유량의 높은 순서는 메기, 가물치, 붕어, 잉어이고, 이들 측정값은 규제치에 미달된다. Pb함유량은 평균 0.666~1.984ppm이고 송정 붕어뼈가 가장 높고 동지역 잉어 근육이 가장 낮다. 어종별 Pb함유량의 높은 순서가 메기, 붕어, 가물치, 잉어이고, Pb측정치는 국내외 수산물 규제치에 훨씬 미달한다. Zn함유량은 평균 8.673~15.214ppm으로 외국의 규제치 40~50ppm에 훨씬 미달한다. 어종별 Zn함유량의 높은 순서는 붕어, 메기, 가물치, 잉어이고 송정 붕어 아가미가 가장 높고 나주호 붕어 근육이 가장 낮다. Mn함유량은 평균 2.751~5.049ppm이고 동강 메기 아가미가 가장 높고 나주호 메기 근육이 가장 낮다. 어종별 Mn함유량의 높은 순서는 메기, 붕어, 잉어, 가물치이다. Cu함유량은 평균 0.328~1.174ppm이고 동강 가물치 아가미에서 가장 높고 나주호 잉어근육이 가장 낮다. 어종별 Cu함유량의 높은 순서는 가물치, 잉어, 붕어, 메기 이다. Cu검출량도 규제치 이하이다. Cd함유량은 평균 0.017~0.052ppm으로 오염된 상태가 아니라고 생각된다. 동강 잉어 뼈에서 가장 높고 송정 잉어 근육이 가장 낮다. Cd함유량의 높은 순위의 어종은 잉어, 메기, 가물치, 붕어이다.<br/> 이상의 결과로 영산강 담수어의 중금속 오염실태가 아직은 우려 할 정도가 아닌 것으로 판단된다. This study was performed to investigate the contamination level of harmful metals in 4 species of freshwater fishes collected at the three sampling sites of Yeong san river from January to October in 1990. The levels of total mercury, lead, zinc, manganese, copper and cadmium were determined and the results were summarized as follows : The overall range(ppm) were Hg, 0.022~0.139 ; Pb, 0.666~1.984 ; Zn, 8.673~15.214 ; MD, 2.751~5.049 ; Cu, 0.328~1.174 ; Cd, 0.017~0.052. Among the six heavy metals contents, the highest was zinc and the lowest was cadmium. The results showed that all the 4 species of freshwater fishes of Yeong san river studied have not yet dangerous levels enough to pose a health problem.

불포화도가 다른 식물성 유지를 섭취시킨 흰쥐에서 정상적 간 및 지질대사변화의 고찰

서화중(Hwa-Joong Sheo),김선희(Sun-Hee Kim),정두례(Du-Le Jung) 한국식품영양과학회 1991 한국식품영양과학회지 Vol.20 No.5

Rat에 들깨유, 옥수수유, 올리브유를 28일간 매일 투여하여 rat성장율과 간대사 및 지질대사에 미치는 영향을 측정한 결과는 다음과 같다.<br/> 체중 증가율은 2주째까지는 대조군 11.72~23.07%에 비교한 실험군 모두 7.86~20.89%로 약간 둔화되나 대체로 정상으로 볼 수 있고 3, 4주째 들깨유 투여군(A군)이 7.57~8.63%로 대조군의 17.53~13.81%에 비해 현저히 감소되고 기타 B, C군은 대조군과 거의 같은 수준이다. 혈액의 생화학적 검사결과에서 GOT, GPT값은 전 4주간 대조군의 평균 158.8와 178.18u와 비교한 B, C군은 각각 148.1~72.5u와 153.71~83.58u로 별 영향을 없으나 들깨군(A군)은 GOT, GPT가 174.87과 93.46u로 대조군 보다 약간 증가된 값이다. Alk-P는 대조군의 58.5u에 비교한 전 실험군의 평균 58.62~61.95u는 별차이가 없다. Cholesterol과 triglyceride값은 시료의 불포화도 증대에 비례하여 감소하는 경향이고 대조군의 cholesterol과 triglyceride값 60.33㎎/dl~50㎎/dl와 비교한 A, B, C군이 각각 54.6~36, 61.89~44, 63.71~51㎎/dl을 보여 큰 폭의 차이는 없었다. 간조직 검사 결과 A, B, C군 모두 4주째 1필에서 kupffer cell의 증식내지 활동도 증가를 보이고 특히 들깨유군의 경우는 담관의 팽대, 옥수수군은 vesicular nuclei의 관찰, 올리브유군은 mild fatty metamorphosis가 관찰되었으나 나머지는 대부분 정상이었다.<br/> 이상의 결과에서 들깨유, 옥수수유, 올리브유를 1개월간 rat ㎏당 1.57㎖을 매일 투여하니 유지의 불포화도의 증가에 따라 혈중 중성지방과 cholesterol 농도의 근소한 감소를 보였지만 들깨유의 4주간 투여군은 간기능에 다소 부담을 주는 것으로 보이나 대부분 실험군은 별다른 증상을 보이지 않았다. 따라서 들깨유의 장기간 복용은 간기능에 다소 유해적으로 작용할 것으로 보인다. To investigate the effect of the unsaturated plant oil on the normal liver and lipid metabalism rats were fed the daily dose of 1.57㎖/㎏ body weight of perilla oil (Iodine value 190~207), corn oil (Iodine value 115~130) and olive oil (Iodine value 80~85) respectively for 28 days. The results were as follows. For the 14 days all the test groups showed normal weight gain with 7.86~20.89% increase rate. In the period of the 3rd and the 4th week the increase rate of the perilla oil group was decreased significantly under 17.53~13.8% of control level, but the remainders(corn oil, olive oil) keep normal. The feeding of corn and olive oil for 28 days did not show any harmful effect on normal GOT, GPT activity, ALK-P, serum cholesterol and serum triglyceride value of rat. The perilla oil feeding for 21~28 days slightly increased the GOT, GPT activity as 174.87, 93.46u but decreased the cholesterol and triglyceride value as 54.6~0.36㎎/dl compared to control. In the pathological finding of test group liver some rats in 28days feeding group showed reactive change of kupffer cells, and were noted dilated bile duct in a rat of perilla group, and were seen vesicula nuclei in corn oil group and mild fatty metamorphosis in olive oil group. But most subjects did not show any characterized sign of acute or subacute liver damage.

枸杞子 抽出物이 家兎의 實驗的 肝臟障碍 및 alloxan糖尿病에 미치는 影響

서화중(Hwa-Joong Sheo),전성주(Sung-Joo Jun),이명열(Myung-Yul Lee) 한국식품영양과학회 1986 한국식품영양과학회지 Vol.15 No.2

枸杞子抽出物을 試料로 mouse의 毒性實驗, 實驗的으로 誘發시킨 家兎의 肝臟機能障碍 및 alloxan負荷糖尿病에 미치는 影響을 實驗하여 다음과 같은 結果를 얻었다.<br/> 1. Mouse의 急性毒性實驗에서 腹腔投與의 경우 平均致死量은 12.7g/㎏이었다.<br/> 2. CCl₄로 肝臟機能障碍가 誘發된 家兎에 枸杞子抽出物投與는 對照群에 비하여 有意性있는 回復度를 보였는데 特히 大量投與群에서 더욱 빨랐다.<br/> 1) GPT活性度 및 total bilirubin은 試料投與群에서 아주 優秀한 低下效果를 나타냈다. 特히 GPT活性度에서 200㎎/㎏은 第8日째부터, 800㎎/㎏은 第6日째부터 有意性이 있었다.<br/> 2) Alkaline phosphatase値는 對照群에 비하여 커다란 차이를 보이지 않았다.<br/> 3) Alloxan으로 誘發한 家兎의 高血糖에 대하여 800㎎/㎏는 아주 탁월한 血糖降下效果를 나타냈다. 卽 第4日째부터 對照群에 비하여 有意性이 있었으며 第12日째는 正常値에 거의 接近하였다. 또한 GPT活性度는 차츰 低下되어 第6日째부터 低下效果가 뚜렷하였다. These studies were carried out to investigate effects of Lycii fructus extract on experimentally CCl4-induced liver damage and alloxan-induced diabetes in rabbits and acute toxicity using mice.<br/> LD_(50)(㎎/㎏) of Lycii fructus extract(L.F.E.) was 12.17g/㎏ by intraperitoneal administration in mice.<br/> L.F.E. showed more rapid recuperation compared to the control group in CCl4-intoxicated rabbits and 800㎎/㎏ was the most effective.<br/> Especially GPT activity and total bilirubin level showed an apparant decreasing effect within 6 days and 8 days, respectively in 800㎎/㎏.<br/> But any differences were not observed in alkaline phosphatase and total cholesterol between sample and control group.<br/> Large amount administered group exhibited more excellent hypoglycemic effect in alloxan-induced diabetes of rabbits, that is, it was significant to the control group after 4 days and adjacent to the normal level on 12th day. And GPT activity was gradually decreased and showed clear decreasing effect after 6 days.<br/> It is suggested that L.F.E. can be administered not only as therapeutic agents (such as liver tonics or antidiabetetics) but also a natural food to shorten the recovery time of hepatic function in liver diseases and decrease the abnormally elevated blood glucose such as Diabetes Mellitus.

益母草綠汁이 家兎의 肝臟障碍 및 高血糖에 미치는 影響

서화중(Hwa-joong Sheo),국현숙(Hyun-sook Kook),이명열(Myung-yul Lee) 한국식품영양과학회 1987 한국식품영양과학회지 Vol.16 No.1

These studies were conducted to investigate the effects of Leonurus sibiricus extract (L.S.E.) on experimentally CCI₄-induced liver damage and alloxan-induced diabetes in rabbits;<br/> Leonurus sibiricus juice (L.S.J.) showed the most rapid recuperation of liver function among samples compared to the control group in CCI₄-intoxicated rabbits. SGPT activity showed an apparant decreasing effect in 4 days, and the blood level of total cholesterol and total bilirubin in 6 days, and not in alkaline phosphatase level.<br/> L.S.J. exhibited more excellent hypoglycemic effect in alloxan-induced diabetes of rabbits, that is, significant to the control group after 2 days and adjacent to the normal level on 8th day. And SGPT activity and total cholesterol level were gradually decreased and showed markedly decreasing effect after 4 days.

益母草 綠汁이 흰쥐의 胃液分泌機能에 미치는 影響

서화중(Hwa-Joong Sheo),이명열(Myun-Yul Lee) 한국식품영양과학회 1986 한국식품영양과학회지 Vol.15 No.4

These studies were carried out to investigate effects of Leonurus sibiricus green juice on gastric secretion of rats and acute toxicity in mice;<br/> LD_(50)(㎎/㎏) of Leonurus sibiricus extract was 625㎎ ㎏ intraperitoneally in mice.<br/> The groups administered green juice and water extract of Leonurus sibiricus showed more excellent increasing effect in total acidity and peptic activity, and decreasing effect on gastric volume than control group.<br/> But any differences were not found between sample and control group in gastric pH.<br/> It is suggested that Leonurus sibiricus can be used as the bitter stomachics to promote the gastric digestion.

梅實抽出物이 家兎의 Alloxan 糖尿病에 미치는 影響

서화중(Sheo Hwa Joong),고은영(Ko Eun Young),이명열(Lee Myung Yul) 한국식품영양과학회 1987 한국식품영양과학회지 Vol.16 No.3

These studies were conducted to investigate effects of Prunns mume extract on experimentally alloxan-induced diabetes in rabbites, antibacterial activity and acute toxicity in mice;<br/> 1. LD_(50) of Prunus mume extract(P. M. E.) was 15.25g/㎏ intraperitoneally in mice.<br/> 2. P. M. E. showed more significant recuperative effect compared to the control group in alloxan-induced diabetes of rabbits.<br/> 1) P.M.E. 800㎎/㎏ bodywelght exhibited more excellent, hypoglycemic effect afte 6 days and adjacent to the normal level at 14th day.<br/> 2) SGPT activity was significantly decreased after 6 days, and the blood levels of total cholesterol and urea-nitrogen were significant in 800㎎/㎏ at 6 days and 10 days respectively.<br/> 3. In antibacterial activity test P.M.E. was active at 0.195㎎/㎖ in Staphyllococcus aureus, 3.125㎎/㎖ in E. coli, Enterobacter cloacae, Klebsiella oxytoca, and in Pseudomonas aeruginosa at 0.391㎎/㎖.<br/> 4. In histological findings, the sample groups were deeply stained, fully granulated and partial degranulation of β-cells, and a few vacuolar and vesicular changes of cytoplasm than alloxan treated group in proportion to the sample amounts.

섬진강 (蟾津江) 하류 (下流) 기수구역 (汽水區域) 수질의 (水質) 이화학적 (理化學的) 조사 (調査)

주흥규,서화중 ( Jhoo Heung Kyu,Sheo Hwa Joong ) 한국하천호수학회 1975 생태와 환경 Vol.8 No.1·2

The study on the water quality of the estuary of Seom Jin river and Gwang Yang bay was carried out for the purpose of environment survey of Gwang Yang bay on May, July, and September in 1974. Under the various atmospheric condition the sample were taken and analyzed by the 13 selected items of chemical and physical standard method. The results are as follow. Ⅰ. Estuary of Seom Jin river (St. 11). This area is greatly influenced by Seom Jin river in water quality and has fairly good and fresh like water quality. Flowing into Gwang Yang bay, this water has a dilution effect of pollution substance and salinity in bay. Ⅱ. North area of Myo island (St. 9, 10). This area is also affected by Seom Jin river but less than St. 11 and especially the chlorine ion content increase suddenly. Ⅲ. East area of Myo island (St. 4, 6). The fresh water of Seom Jin river and sea water out of bay, they equally affect this area, and has a little increasing tendency of pollution as compared with St. 9 and 10. Ⅳ. West area of Myo island (St. 7, 8). Tidal Current in this area is some what delayed on account of being interrupted its flowing by Myo island and there is not any higher polluted station in bay than in this station. Ⅴ. Enterance of bay (St. 1,2,3) and Nam Hae bridge(St.5). Being adjacent to the outer sea, those area are influenced by sea water and show nearly oceanic water quality. ⅥⅦ

영산강 중류 및 상류수계의 ABS 오염도에 관한 연구

안영근,서화중 ( Young Gun Ahn,Hwa Joong Sheo ) 한국하천호수학회 1976 생태와 환경 Vol.9 No.3·4

The contamination level of ABS, the synthetic detergent, was determined at eleven areas of upper streams and mid streams of Young San river on April, May and June in 1976. Throughout the whole experiment Ji Seok, Hwang Ryong and upper Young San river all had below 0. 075ppm ABS residue, and in these area ABS contents were similar to each other. The sudden increasing tendency of ABS contents at the mid stream are attributed to being greatly affected by much polluted Gwang Ju stream which has 0.235∼0.275ppm of ABS. But dilution of pollutant was taken place in this area by the inflowing of Ji Seok and Hwang Ryong river. On June ABS content and other pollutant increased a little as compared with April and May. But at any area it did not exceed 0. 5ppm, the standard value of drinking water. To know the influence of ABS contamination on DO content the relationship between ABS and DO was examined by calculating correlation coefficient. This relationship has strong and significant correlation(r=-0.78) and the distribution trend of frequency are more fit to the curve line equation xy-6.3x=0.045 than to the straight line equation y=-3.9x+7.4. In the relationship between ABS and COD a strong and significant correlation coefficient(r=0. 93) was calculated and the frequency distribute very closely along the straight line equation y=22.2x+1.21. From above facts the author found out that as the ABS contamination in general river water coexist closely with other pollutant it is likely to be considered as new water pollution index.