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밀×야생밀 속간잡종의 genomic in situ hybridization
고종민(Jong Min Ko),한상익(Sang Ik Han),서득룡(Duck Yong Suh),권일찬(Yil Chan Kweon),곽용호(Yong Ho Kwak),서봉보(Bong Bo Seo) 한국육종학회 1997 한국육종학회지 Vol.29 No.4
Genomic in situ hybridization (GISH) was used to discriminate parental chromosomes of 3 intergeneric hybrids such as Triticum aestivum×Thinopyrum junceum, T. aestivum×Th. ponticum and T. aestivum×Leymus karelinii. Biotin labelled T. aestivum DNA was hybridized to the chromosomes of the three hybrids. In the hexaploid (2n=6x=42) T. aestivum×Th. junceum, 21 chromosomes with origin of T. aestivum were observed yellow-green, and the rest were orange-red. 21 yellow-green chromosomes and 35 orange-red ones were observed in the octaploid (2n=8x=56) T. aestivum×Th. ponticum, while the heptaploid (2n=7x=47) T. aestivum×L. karelinii was clearly distinguished as having 21 yellowgreen and 28 red chromosomes. Some of 35 orange-red chromosomes of Th. ponticum have bands with yellow-green, indicating that some regions of chromosomes of Th. ponticum were hybridized with T. aestivum DNA used as probe. The results showed that GISH could identify chromosomes of different origin from the hybrids.
밀 F₁ 약배양에 의한 식물체 분화 및 분화식물체의 배수성
권순종(Soon Jong Kweon),박동수(Dong Soo Park),고종민(Jong Min Ko),현종내(Jong Nae Hyun),서득룡(Duck Yong Suh),서형수(Hyung Soo Suh),남중현(Jung Hyun Nam),홍병희(Byung Hee Hong),오윤진(Yun Jin Oh) 한국육종학회 1997 한국육종학회지 Vol.29 No.2
Five combinations of early maturity wheat F₁ were conducted to anther culture and 345 green plants were obtained. Callus induction rate was 4.1%, plant regeneration rate 46%, green plant rate 88%, respectively. Liquid medium was effective to induce callus of wheat anther. Chromosome analysis of 102 plants showed 5 diploids, 1 aneuploid and 96 haploids. For breeding with anther culture in wheat, chromosome doubling process will be necessary.
박동수,서득룡,서봉보,고종민,도금숙 한국유전학회 2000 Genes & Genomics Vol.22 No.4
Many progenies were produced from the backcross of Triticum aestivum cv. Olmil×Secale cereale cv. Paldanghamil hybrid using Olmil as the male parent. Nineteen ditelosomic rye chromosome addition lines (1RL) were selected in the BC_1F_6 generation by cytogenetic and genomic in situ hybridization (GISH) analysis. Progenies of BC_1, which were derived from one seed, were advanced to BC_1F_6 generation. The percentage of seed set in backcross derivatives of T. aestivum cv. Olmil×S. cereale cv. Paldanghomil was enhanced in progression, after each generation. GISH technique was applied to select the plants having the rye chromosome in BC_1F_6 generation. The presence of rye chromatin was identified in 32 out of 467 plants in 77 BC_1F_6 families. GISH analysis showed that the modes of rye-chromatin in BC_1F_6 plants were almost telocentric addition lines including nineteen ditelocentric and ninety two monotelocentric rye chromosomes. The added ditelocentric chromosomes were found as pairs and underwent normal meiotic pairing and segregation, while monotelocentric chromosomes showed meiotic irregularities.
Ti-플라스미드 유전자 운반체에 의한 대두 형질전화 식물체에 있어서 β-Glucuronidase 유전자의 유전양상
김달웅,조은기,서득룡,오웬스, L . D . 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
To introduce β-glucuronidase (GUS) gene of Escherichia coli into plant cells using an Agrobacterium/binary vector system, we cocultivated shoot apices of soybean with Agrobacterium. After 3 weeks of culture on a culture medium containing 100 ㎎/L kanamycin, kanamycin resistant apices were selected. High GUS activities were detected in transformed explants by spectrophotometric assay. A tumefaciens ET₂[pGT100/EHA101] strain was more effective than the other strains for GUS expression from kanamycin resistant shoots. GUS was expressed in 43% of the cultured explants. It took only 6 weeks to regenerate the rooted plants. When transformants were self-pollinated, GUS gene was inherited to the next generation. Segregation ratio for GUS in T₂ suggests the insertion of a single copy of GUS.
Factors on Somatic Embryogenesis and Organogenesis of Soybean (Glycine max)
Kim, Kyong-Ho,Oh, Young-Jin,Suh, Sug-Kee,Kim, Hag-Sin,Kim, Tae-Soo,Lee, Mi-Ja,Park, Ho-Ki,Suh, Duck-Yong,Yeo, Up-Dong 한국콩연구회 2002 韓國콩硏究會誌 Vol.19 No.1
To enhance in vitro plantlet regeneration efficiency of soybean through embryogenesis, the culture conditions such as meterial part and size of immature seed, 2,4-D pH and solidifying agents for somatic embryogenesis were investigated. Somatic embryogenesis was induced from the immature embryo, immature cotyledon, and embryonic axis explants of the immature seed on MS medium supplemented with 2.0 mg/L 2,4-D. The highest rate (up tp 23%) of somatic embryogenesis was obtained from the immature cotyledon, followed by embryonic axis and immature embryo. The rate varied with the developmental stages of seed. The maximum rate (25%) of embryogenesis was obtained from 3-4 mm length of the seed after 25 days of flowering. The optimum concentration of 2,4-D for embryogenesis was 10mg/l. The optimum pH was at 5.8 and solidifying agent for medium was better with 0.4% gelrite than with agar. For rapid multiplication of shoot tip from germinating somatic embryos, they were cultured on MS medium containing 2 mg/L IBA and 1 mg/L BAP, After then somatic embryos with one and three cotyledons were transferred to the plant growth regulator free medium. The medium exhibited the higher rate (50%) of development than the multiplication medium. To select the section with shoot formation ability, the calli and shoot formation from three sections of 5-days-seedings of soybean were induced on MS medium supplemented with 1.0 mg/L BAP, 3% sucrose, and 0.3% gelrite for one month. The first leaf section exhibited the highest shoot formation rate (51%), followed the hypocotyl section and the cotyledon section. The shoot formation rates and shoot number of the four excised sections of the first leaf were aldo investigated on the same medium. A half of the first leaf explant and the third of the first leaf explant had higher shoot formation rates (76-80%) and number (3-4/ explants) than those in other two explants. Effects of cytokinins on shoot formation were determined, using the half of the first leaf explants. Zeatin (1.0mg/L) exhibited the highest in shoot formation rate (94%) and combination (1: 1, v/v) of zeatin (1.0mg/L) and IAA (1.0 mg/L) eshibited the highest in shoot formation rate (96%) and numbers (16/explant), twice more than zeatin alone (1.0 mg/L). The shoot cuttings were transferred and cultivated on the rooting media supplemented with only auxin, IBA at variois concentrations. The highest root formation (8/shoot) was achieved on the medium supplementes with 1.5 mg/L. After 4 weeks of cultivation, the plantlets with an extensive root system were transplanted in pots with a soil mixture of vermiculite and fine sand. Transferred to field, about 75% of the plantlets survived.