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돼지의 혈청 progesterone 수준에 의한 임신진단에 의한 연구
김상근(S . K . Kim),김무강(M . K . Kim),이규승(K . S . Lee),서길웅(K . W . Seo),김창식(C . S . Park),박항균(H . K . Park) 한국축산학회 1989 한국축산학회지 Vol.31 No.1
The study was carried out to investigate the changes of hormone levels in blood serum during the estrous cycle and the accuracy of pregnancy diagnosis by estimation of plasma progesterone at 20 to 23 days after breeding in sows. The progesterone and estradiol-17 R from blood serum samples were assayed by radioimmunoassay method. The results of this study were summarized as follows: I.The progesterone concentrations in blood serum during the estrous cycle began to decline rapidly at 2 days before estrus, and thereafter decreased to 0.7±0.18ng/ml at on the day of estrus, and thereafter reached a peak mean level of 13.1±2.15ng/ml at 15 days after estrus. 2.The estradiol-17β concentrations in blood serum during the estrous cycle showed a peak mean level of 14.6±3.24pg/ml at 18 days after estrus, and decreased from 9.8 pg/ml to 14.2 pg/ml at luteal phase. 3.During 20 to 23 days after artificial insemination, the accuracy of pregnancy diagnosis from the blood serum progesterone levels were 96.4% for non-pregnant sows($lt;2.7ng/ml), and 86.1% for pregnant sows( 3.6ng/ml). The average overall accuracy of pregnancy prediction for non-pregnant and pregnant sows was 91.3%.
한국재래산양정자 (韓國在來山羊精子) 의 액상 및 동결보존에 관한 연구
박창식,양문한,황덕수,이규승,서길웅 ( C . S . Park,M . H . Yang,D . S . Hwang,K . S . Lee,K . W . Seo ) 한국축산학회 1989 한국축산학회지 Vol.31 No.7
This study was carried out to develop the preservation methods of fresh and deep frozen Korean native goat spermatozoa and investigate the farrowing rate of Korean native goat does inseminated with frozen-thawed semen. The results obtained were as follow. 1. Motility and NAR acrosome of fresh spermatozoa during incubation were higher at 22℃ than at 5℃ or 37℃(p $lt;0.01). The percent LAC was inversely related to the percent NAR with regard to the incubation periods of these same three treatments. 2. Motility and NAR acrosome of spermatozoa diluted to 1:4 during incubation were higher at 22℃ than at 5℃ or 37℃. Especially incubation for 0.5, 3 and 6 h in the 5℃ had a marked detrimental effect on motility and NAR acrosome of spermatozoa. 3. Motility and NAR acrosome of spermatozoa during incubation were higher for samples diluted 1:1, 1:2, or 1:4 than for samples diluted 1:6(p $lt;0.01). 4. Motility and NAR acrosome of post-thaw spermatozoa were higher at freezing rate of 12℃/min or 24℃/min than at freezing rate of 1℃/min(p $lt;0.01). 5. Motility and NAR acrosome of post-thaw spermatozoa at freezing rate of 12℃/min were higher at 9% glycerol than at 6% or 3%(p $lt;0.01). 6. After thawing of frozen spermatozoa in a 37℃ water bath for 10 sec, incubation temperatures of 12℃, 22℃ or 37℃ for 2h did not show any differences on motility and NAR acrosome. 7. Farrowing rate of Korean native goat does inseminated twice with frozen-thawed semen was 25.0%. Mean kid weight at birth was l.9kg.