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경북지역 산란계에서 Avian pneumovirus에 대한 항체가 및 바이러스 검출 조사
김정은 ( Jung Eun Kim ),황지영 ( Ji Young Hwang ),배동록 ( Dong Rok Bae ),성명숙 ( Myoung Suk Sung ),김순태 ( Soon Tae Kim ),김상윤 ( Sang Yun Kim ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1
Avian pneumovirus(APV), also known as avian rhinotracheitis(ARTV), affects both turkeys and chickens and is known to be the primary causative agent of turkey rhinotracheitis(TRT). The aim of this study was to establish the presence or absence of antibodies to APV by an enzyme-linked immunosorbent assay(ELISA) and confirm APV by a reverse transcriptase-polymerase chain reaction(RT-PCR). The tested serum and feces were collected from laying hens in Gyeongbuk province. The positive farms with antibody against APV by ELISA were 90(96.7%) of 93 and positive serum samples were 433(93.1%) of 465 different sera. By regional group, sera from Uiseong, Cheongsong and Bonghwa were noted as 100% positive and positive rates of samples from Yeongju, Andong and Yeongyang were 93.3%, 85.7% and 50%, respectively. However, APV was not detected in feces samples by RT-PCR.
육계에서 조직과 혈액의 enrofloxacin 및 대사성 ciprofloxacin 잔류 상관관계 조사 및 검사방법간의 비교
성명숙 ( Myung Suk Sung ),김미숙 ( Mi Suk Kim ),서희진 ( Hee Jin Seo ),배동록 ( Dong Rok Bae ),황지영 ( Ji Young Hwang ),김순태 ( Soon Tae Kim ),조종숙 ( Jong Suk Cho ),박홍제 ( Hong Je Park ),홍성희 ( Sung Hee Hong ),김경동 ( Gyu 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.4
The purpose of this study was to evaluate detecting methods and the relationship between tissues and blood for enrofloxacin and metabolic ciprofloxacin residues in broiler chickens. Two groups of broiler chickens were administrated via the drinking water with 50 μg/mL and 100 μg/mL of enrofloxacin for 5 days, respectively. The concentration of enrofloxacin and metabolic ciprofloxacin in tissues (muscle and kidney) and blood were measured during administration period (for 5 days) and withdrawal period (for 12 days) by high performance liquid chromatography (HPLC) method. Also, all samples were conducted for screening of residues by microbial method using E. coli for quinolone detection and immuno- chromatography method using Smart kit. The relationship between tissues (muscle and kidney) and blood for enrofloxacin and metabolic ciprofloxacin residues in broiler chickens was followed : The levels of enrofloxacin and metabolic ciprofloxacin residues in muscle and kidney were higher 2.9∼3.2 folds, 3.6∼3.8 folds more than the residues levels in blood, respectively. These results support we can predict the residues in muscle and kidney from the residues in blood. In comparison of detecting methods for antibiotic residues, microbial method using E. coli for quinolone detection and immuno-chromatography method using Smart kit could detect positive reaction at similar or lower concentration than violative concentration of enrofloxacin and metabolic ciprofloxacin in chicken tissues. These results support what two screening methods are useful for screening of quinolone detection in chickens.
돼지 및 닭에서 분리된 균에 대한 봉독의 최소억제농도(MIC)
김순태 ( Soon Tae Kim ),황지영 ( Ji Young Hwang ),성명숙 ( Myoung Suk Sung ),제소양 ( So Yang Je ),배동록 ( Dong Rok Bae ),한상미 ( Sang Mi Han ),이성해 ( Sung Hae Lee ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.1
Bee venom is synthesized in the venom gland`s of worker and queen bees and stored in their venom sacs. Bee venom is a rich source of enzymes, peptides and biogenic amines. there are at least 18 active components in the venom which have some pharmaceutical properties. This study was performed to evaluate minimum inhibitory concentration(MIC) of bee venom against bacteria isolated from pigs and chickens with disease. In case of reference strains, the MIC(㎍/㎖) of Staphylococcus aureus ATCC 6538, Streptococcus mutans ATCC 25175, and Salmonella typhimurium ATCC 6538 were 64, 64 and 32, respectively. In case of bacteria isolated from pig and chicken, the MIC of Staphylococcus aureus, Staphylococcus hyicus and Staphylococcus chromogenes were 8, 128 and 128, and that of 11 strains of Escherichia coli were 8 to >512 and that of 8 strains of Salmonella spp were >512. Antibacterial resistance test of 22 strains isolated from pig and chicken and 3 reference strains were performed by agar gel diffusion method, using 17 antibacterial drugs including penicillin, cefazolin, tetracycline and quinolone group. The multiple drug resistant patterns were found in most strains isolated from pig and chicken.