백반증 피부 병변의 최소광독량에 관한 연구

박장규 ( Jang Kyu Park ),김연수 ( Youn Soo Kim ),노영우 ( Young Woo Rho ),윤소영 ( So Yeong Yoon ),김태원 ( Tae Won Kim ),이웅재 ( Woong Jae Lee ),서기범 ( Ki Beom Suhr ),이증훈 ( Jeung Hoon Lee ) 대한피부과학회 1998 大韓皮膚科學會誌 Vol.36 No.2

Background: It is mandatory to measure the minimal phototoxic dose(MPD) in order to determine adequate irradiation of UV-A in photochemotherapy. However, the measurement of MPD is not easy in some cases due to inadequte size and site of lesions, time and manpower. Objective : The purspose of this study was to standardize the minimal phototoxic dose for a vitiliginous lesion. Methods : The minimal phototoxic dose of UV-A was measured in 82 vitiligo patients. Then we analyzed the MPD according to the sex, age, site of the vitiliginous lesions, duration of disease, and administration route of the photosensitizer. Results : 1. There were no significant differences between exposed and unexposed areas in MPD in both cases of topical and systemic administration of the photosensitiser. 2. There was no significant correlation between disease duration and MPD in both cases of topical and systemic administration of the photosensitiser. 3. In the group of topical application of 8-methoxypsoralen cream, MPD for males and females were 0.53+0.38J/cm2 and 0.48+0.32J/cm2 respectively without significant difference in sex. 4. In the group of systemic administration of 8-methoxypsoralen, there was a significant difference between males and females in MPD. The MPD in males and females were 1.38+0.72J/cm2 and 2.51 + 1.40J/cm2, respectively. 5. In the group of topical application of 8-methoxypsoralen cream, the MPD in 2nd decade patients was the highest(0.80+0.55J/cm2). The MPD had a tendency to decrease gradually as age receded from the 2nd decade. 6. In the group of systemic administration of 8-methoxypsoralen, there was a tendency for the MPD to be increased according to age without statistical significance. 7. MPD in the group systemically administrated with 8-methoxypsoaralen was four-fold to that of the topical application group. Conclusion : It may be valuable to keep in mind that there was a statistical differrence in MPD between sexes in systemic administration of the photosensitizer, and that MPD was different according to patients age in the topical application group. (Korean J Dermatol 1998;36(2): 241-245)

Neutral red 염색을 이용한 피부사상균의 생육성 평가

박장규 ( Jang Kyu Park ),이우재 ( Woo Jae Lee ),서기범 ( Ki Beom Suhr ),이증훈 ( Jeung Hoon Lee ) 대한피부과학회 1996 大韓皮膚科學會誌 Vol.34 No.1

Background: Microsopic examination of potassium hydroxide(KOH) preparation and fungus culture is required for the diagnosis of fungal infection. Sometimes dermatophytes fail to grow on culture medium, although these are observed on microscopic examination of KOH preparation. Recently this discrepancy between microscopic examination and fungus culture can be explained by the hyphothesis that some of the fungal elements are non-viable. Object: This study was made to evaluate the viability of dermatophytes using neutral red(NR) staining for the explaination of discrepancies between microscopic examination and fungus culture. Methods : After identification of fungus by culture from dermatophytic lesion the hyphae was collected for this study. In order to confirm whether the NR staining is suitable for check the viability of hypae or not, we designed to prepare the preparations of hyphaes by 2 ways. One was killed hyphae by autoclave, the other was kept as viable hyphae. And then we compared the stainability of NR staining and autoradiographic study using H-thymidine. And we compared the results of NR staining and the subsequent culture using the scales which were collected from the lesions 30 dermatophytic patients. Results : The structure inside of cell wall of hyphae stained red color only in case of viable hyphae preparations, but not stained in killed hyphae preparation. Autoradiographic study using H-thymidine confirmed that grain-positive cells(viable cells) were stained with NR, whereas grain negative cells (non viable cells) were not stained. Among the 30 cases with dermatophytosis 27(90.0%) cases showed NR positive and 14(46.6%) cases showed culture-positive. Except the tinea unguium cases which have shown low culture positive rate, 9(75.0%) cases of the 12 NR positive samples were positive on culture. All 14 cases of the culture positive samples were positive on NR staining. And all 3 cases of the NR negative samples were negative on culture. Conclusion : NR staining can be a useful method for the evaluation of viability of the fungal elements. (Kor J Dermatol 1996;34(1): 122-126)

충청 지방의 표재성 피부사상균증의 임상 및 균학적 관찰

박장규(Jang Kyu Park),이우재(Woo Jae Lee),김경훈(Kyung Hoon Kim),서기범(Ki Beom Suhr) 대한피부과학회 1995 大韓皮膚科學會誌 Vol.33 No.1

Objective : The study was made in order to inverstigate the clanical and mycological status of dermatophytosis in Chungchong province, incidence and friquency of disease, sex and month distribution of the disease, varieties of the causative organism. Methods : We performed clinical and mycological studies of 86 cases of dermatophytosis which have showed positive on KOH examination and/or positved result in culture among out- patients of Dermatologic Clinics of Chungnam National Univesy during 7 years, from 1986 to 1993. Results & Conclusion : 1. The ratio of male to female patients 330: 256 and make little ifference. 2. The incidence rate was bighest in the third decade(25.4%) 3. The seasonal prevalence of dermatophytosis was highest in the summer 4. The incidence of dermaiophytosis was T. pedis(30.2%), T. (22.9%), T. ungium(13.1%), T. corPoris(10.1%), T. capitis(9.0% ) in decreasing order of freqercy 5. A mean positive rate for the KOH mount examination A. 5. 83.8%, T. 90.2%, T. corporis 89.8%, T. ungium 86.5% in decreasing order of frequency 6. A mean positive rate of culture was 77.1%; T. capities 94.3, . faciale 87.0%, T. cruris 84.3 %, T. cpor6 78.0% in decreasing order of frequency 7. T. rubrum was the mot common causative organism of cernatophytosis(69.5%), with T. mentagroPhyte(16.1%), M. canis(13.5%), M floccosum(0.7%), 4 gypseam(0.2%) presenting in decreasing order of frequency (Kor J Dermatol 1995; 33(1): 92-93)

피부 및 내장에 전형적 소견을 보인 악성 위축성 구진증 1 예

서기범(Ki Beom Suhr),이증훈(Jeung Hoon Lee),박장규(Jang Kyu Park),김정수(Jeong Soo Kim),김경호(Kyung Ho Kim),박민수(Min Su Park),송인상(In Sang Song) 대한피부과학회 2002 대한피부과학회지 Vol.40 No.1

A 33-year-old male patient had malignant atrophic papulosis that affected the skin and intestinal tract and probably respiratory system. He had multiple typical skin lesion with porcelain-white atrophic scars on the whole body surface area except on face, palm and sole. And he had been operated two times due to intestinal perforation after onset. Multiple whitish necrotic patches were found in small and large intestine during laparotomy. Also he had a plueral effusion. Histopathologic studies showed atrophic epidermis and dermal necrosis, vascular change, mucin deposition in lower dermis. He was treated with acetyl salicylic acid and dipyridamole, but there was no improve-ment. He died of intestinal perforation and respiratory failure 67 months after onset(6 months after onset of abdominal symptom). (Korean J Dermatol 2002;40(1):48~53)

흡입수포액 및 냉동수포액이 섬유아세포 성장에 미치는 영향에 관한 연구

서기범(Ki Beom Suhr),김경훈(Kyung Hoon Kim),이우재(Woo Jae Lee),박장규(Jang Kyu Park) 대한피부과학회 1995 大韓皮膚科學會誌 Vol.33 No.1

Beckground : Wound fluid is believed to contain growth factors produced by cells involved in the healing process. One of the beneficial effect of occlusived ressing is the retention of released fluid from the wound, which is thought ta accelerate wound healing. However, there was no report how suction-induced or cryoinduced bulla flicmight affect the healing of the wound itself. Objective : Therefore, we have examined the effect of sucior-or cryo-induced bulla fluids on the in vitro growth of buman dermal fibroblasts. Methods : Suction-or cryo-indueed bulla fluid were sterilel cillected from the suction blisters and cryotherapy induced bulla, respectively. After seed n in optimal growth media on day 0, cultures of normal fibroblasts were supplemented with various concentrations of human serum, suction-indaiced bulla fluid, and cryoinduced bull fiuid on day 2, 4, 6, 7 or 8. Results : As determined by cell counts or H-thymidine inorioration, the effect of human serum were much more than those of suction-induced bulla flazic on the growth of fibroblasts. Although slight mitogenic effects were observed in the case of 5 year yoinduced bulla fluid, 10 % cryoinduced bulla fluid inhibited the growth of the fibroblest, as well. Conclusion : These results suggested that suction-induced ulla fluid was not a whole human serum but a dilutet serous material having limited stipulated effect to the growth of the fibroblasts, and cryoinduced bulla held the unknown in laboratory or toxic factors as well. (Kor J Dermstol 1995; 33(l): 85-91)

충청 지방에서 발생한 Trichophyton verrucosum에 의한 두부 독창

노영우,이우재,서기범,이증훈,박장규 ( Young Woo Ro,Woo Jae Lee,Ki Beom Suhr,Jeong Hoon Lee,Jang Kyu Park ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.1

In Korea, Kerion celsi caused by T. verrucosum was first reported by Kim et al in the Honam province in 1986. Since then three mare cases have been reported in the Young Nam province. Herein present a case of kerion celsi caused by T. verrucosum from Chungcheong province. The patient, a 7-year old female, showed a 4x 4cm sized, oval, yellow crusted and boggy patch with alopecia on the scalp. She was living on a farm in a rural area of Chungcheong province. Cows on the farm also exhibited similar skin lesions on the buttock. The same T. verrucosum was identified by a characteristic colony morphology on Sabourauds dextrose agar and chains of chlamydoconidia with mycologic stain from scales of lesions of patient and cows. This skin lesion was successfully treated with griseofulvin. (Kor J Dermatol 1997;35(1): 187~190)

투명세포 한선종 2예

노영우,김경훈,서기범,박장규 (Young Woo Ro,Kyung Hoon Kim,Ki Beom Suhr,Jang Kyu Park) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.2

We report here two cases of clear cell hidradenoma. The first patient was a 19-year-old adolescent, who had single, pinkish, nodule on the left cheek for 1 year. The second case of 70-year-old woman had multiple projecting, pinkish tumor mass with serous discharge on the right inframammary area for 20 years. On histopathological examination, we could observe polyhedral cells and clear cells with tubular differentiation in both cases and large cystic changes due to tumoral degeneration in the second case, The tumor masses were excised, and have not recurred. (Kor J Dermatol 1996;34(2): 300-303)

헬륨 - 네온 레이저 조사가 단층 ( monolayer ) 과 교원질 격자 ( Collagen lattice ) 에 배양한 인체 섬유모세포의 증식과 교원질 합성에 미치는 영향

서기범,윤소영,이우재,이증훈,박장규 (Ki Beom Suhr,So Young Yoon,Woo Jae Lee,Jeung Hoon Lee,Jang Kyu Park) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.2

Background: Recently it has been suggested that lasers can modulate the biological functions of cells in vitro. It has also been reported that a helium-neon(He-Ne) laser can stimulate wound healing in the absence of thermal effects. However, the results of more recent studies on the influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblasts have been inconclusive. And most experiments have not been performed in a three-dimensional collagen lattice that is the physiologic model for an in vitro experiment. Objective : In this study we have investigated the nature of the influence of He-Ne laser irradiation on the proliferatior and collagen synthesis of human dermal fibroblasts cultured in a monolayer and collagen lattice. Methods : We used a 10mW He-Ne laser emitting a beam of wavelength 632,8nm. The human dermal fibroblasts were subjected to laser treatment at various energy densities, and the treatment schedule included one daily exposure on three consecutive days. DNA replication was assessed by H-thymidine incorporation, and the collagen production was monitored by the synthesis of H-hydroxyproline following incubation of the culture with H-proline. Results : The results were as follows : In the control group, the proliferation and collagen synthesis of fibroblasts sultured in a collagen lattice on day 4 and 7 were significantly reduced compared with conventional manolayer cultures. Although the proliferation of human dermal fibroblasts was not remarkable in all experimental irradiation energy except 4 J/cm in a monolayer culture, a statistically significant stimulating effect on fibroblasts proliferation in collagen lattice were noted on days 4 and 7 with an energy density of 1.5J/cm. And we also found that a great er increase of collagen synthesis of human dermal fibroblasts occurred in a monolayer culture on days 4 and 7 in all irradiated energy densities than those in a collagen lattice culture. But statistically significant enhancement of collagen synthesis was showed only at the energy density of 1 J/cm in a collagen lattice culture on days 4. Conclusion : These data indicated that the influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblasts cultured in a collagen lattice was remark ably different from those in a monolayer culture. In the clinical application of the He-Ne laser, the control of the amount of irradiation of the He Ne laser may regulate the proliferative activity and collagen synthesis of human dermal fibroblasts in wound healing. (Kor J Dermatol 1996;34(2): 279-288)

섬모기질종 25예의 임상 및 병리조직학적 연구

서기범,이우재,윤지석,이증훈,박장규 ( Ki Beom Suhr,Woo Jae Lee,Ji Seog Yoon,Jeung Hoon Lee,Jang Kyu Park ) 대한피부과학회 1994 大韓皮膚科學會誌 Vol.32 No.1

피부 편평세포암의 임상 및 병리조직학적 관찰

김윤동(Yoon Dong Kim),서기범(Ki Beom Suhr),이증훈(Jeung Hoon Lee),박장규(Jang Kyu Park) 대한피부과학회 2002 大韓皮膚科學會誌 Vol.40 No.1

N/A Background:The cutaneous squamous cell carcinoma(SCC) is a malignant proliferation of the keratinocyte of the epidermis. It may have the potential to metastasize distally in contrast to the cutaneous basal cell carcinoma (BCC). Our lives were being lengthened gradually and our society was being polluted by many causative factors. Because many kinds of cutaneous malignant tumors occurred to the aged and resulted from the severe pollution, a lot of people have been interested in SCC. Objective:In this study we investigated the recent trend of the cutaneous squamous cell carcinoma in clinical and histopathological aspects. Methods:Seventy one cases of SCC who had visited the Department of Dermatology, Chungnam University Hospital during the last 17 years(between 1983 and 1999) were confirmed in histopathological review. The observation of occurrence rate in years, age and sex distribution, onset, site, cause, histopathological malignancy and treatment was done, retrospectively. Results:1. The occurrence of the cutaneous squamous cell carcinoma in males was one and half times more than in females(M>F:1.63x). Age distribution showed almost above 60 years old(about 74%). 2. The common sites (in decreasing order) were face(56%), extremities(upper>lower), other sites and most etiologic factors were idiopathic(71%), in 2cases metastatic. 3. Histopathologically, the subtype of SCC was verrucous(7%), acantholytic(10%), spindle cell(6%), and the degree of differentiation was well-, moderate-, poor-differentiated (respectively 25%, 32%, 20%), and SCC invaded to full dermis(84%) and subcutis(9%). 4.Various treatments were done and among them the simple excision(53%) was common. 5.case recurred and four cases metastasized to adjacent lymph node. One case expired. Conclusion:We investigated the recent trend of the cutaneous squamous cell carcinoma through our cases of SCC who had visited the department of dermatology, chungnam university hospital. (Korean J Dermatol 2002;40(1):25~30)