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환경수 내의 다양한 Ca²+ 농도에 따른 시클리드(Maylandia lombardoi )의 생리적 변화
문혜나 ( Hye-na Moon ),여인규 ( In-kyu Yeo ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.2
We investigated physiological changes in the freshwater cichlid, fish Maylandia lombardoi in response to different calcium concentrations in rearing water. Four different calcium concentrations (0, 2, 4 and 8 mM) were prepared in rearing water by the addition of ionized calcium (CaO) to examine the effect of various calcium concentrations in rearing water on physiological changes in the cichlid fish, M. lombardoi. Total calcium concentrations in plasma and body increased in a calcium concentration-dependent manner and reached the maximum at 8-mM calcium concentra-tion. Stress-related cortisol was significantly decreased in the 8-mM group compared to the control group (0 mM). Lysozyme activity also significantly decreased in the 8-mM group. These results suggest that CaO in rearing water increases calcium uptake in fish and affects the body by decreasing stress and improving immunity in fish.
CaO (oxide calcium)가 첨가된 사료 공급이 넙치 (Paralichthys olivaceus)에 미치는 생리적 변화에 대한 연구
문혜나 ( Hye Na Moon ),남궁진 ( Gung Jin Nam ),여인규 ( In Kyu Yeo ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.5
This study investigated the physiological effects of a diet containing calcium oxide (CaO) on the olive flounder, Paralichthys olivaceus. The results indicate that the amount of calcium in the blood was higher in the calcium group compared with the controls. The aspartate amino transferase (AST) and alanine amino transferase (ALT) levels were lower in the calcium group, while lysozyme activity was higher in the calcium group. Histologically, fish in the cal-cium group had more hepatocytes in the liver and more intact scales. In addition, an attack experiment using Vibrio anguillarum (KCTC-2711) and Edwardsiella tarda (KCTC-3657) showed that the survival was higher in the calcium group compared with the controls. These results suggest that feeding fish CaO when they require it, such as during periods of gonadal maturation and rapid growth, will increase their immunity and resistance to pathogens.
사육수의 pH변화가 숭어(Mugil cephalus)에 미치는 생리적 영향
문혜나 ( Hye-na Moon ),박진희 ( Jin-hee Park ),박천만 ( Chun-man Park ),남궁진 ( Jin Namgung ),김기혁 ( Ki-hyuk Kim ),여인규 ( In-kyu Yeo ) 한국수산과학회 2017 한국수산과학회지 Vol.50 No.2
We examined changes in the physiological responses of gray mullet Mugil cephalus exposed to acidic seawater (pH 6.0, 6.5, 7.0) and normal seawater (pH 8.0, control) for 15 days. As pH decreased, survival rate and body weight also decreased. Levels of aminotransferase, total protein and triglycerides also differed significantly with changes in pH, presumably due to stress caused by exposure to acidic water. The level of osmotic pressure was significantly higher in the pH 6.0 group than in other groups. Superoxide dismutase was significantly higher in the pH 6.5 and 7.0 groups than in the pH 8.0 group, and glutathione level was lowest in the pH 6.0 group. We conclude that decreasing the pH level of seawater induces a stress response in fish, damaging their ability to control their hematological and osmotic pressure. Antioxidant enzymes are generally sensitive to osmotic stress; in this study, antioxidant activity significantly changed with pH level. These results indicate that physiological stress induced by exposure to acidification reduces survival rates and inhibits growth in M. cephalus.
사육수의 pH변화가 복해마(Hippocampus kuda)에 미치는 생리적 영향
박천만(Cheonman Park),김기혁(Ki-hyuk Kim),문혜나(Hye-Na Moon),여인규(In-Kyu Yeo) 한국생명과학회 2017 생명과학회지 Vol.27 No.7
대기의 이산화탄소의 농도 증가는 해양산성화와 지구온난화를 유발하는 것으로 알려져 있다. 해마는 해양생태계 및 수산자원생물로서 중요한 종으로 알려져 있지만, 최근 해양산성화로 인하여 개체수가 감소되고 있는 실정이다. 따라서 본 연구에서는 멸종 위기 종인 복해마(Hippocampus kuda)에 미치는 생리적 영향을 조사하기 위해서 사육수의 산성조건인 pH 6.0, 6.5, 7.0 및 자연해수(pH 8.0)의 환경에서 복해마(H. kuda)를 15일 동안 사육 후 체내조성 변화 및 항산화 효소 활성 변화에 대하여 조사를 실시하였다. 복해마(H. kuda)의 크기 및 성장은 대조군인 pH 8.0을 제외한 실험군에서는 pH가 저하함에 따라 감소하는 경향을 나타내었다. 체내 조성성분인 회분, 조지방 및 조단백 또한 pH 저하에 따라 농도의존적으로 감소하는 것이 관찰되었다. SOD, CAT 및 GSH와 같은 항산화 효소의 분석 결과, SOD활성의 경우, pH 저하에 따라 농도의존적으로 감소하지만, 이와 상반되게 CAT 및 GSH에서는 pH저하에 따라 활성이 농도의존적으로 증가하는 결과가 나타내었다. 이것은 복해마(H. kuda)가 사육수의 pH 저하에 따른 체내 항상성을 유지하는 과정 중 스트레스가 야기되어 에너지 대사가 손상된 것으로 추정된다. 항산화효소는 일반적으로 산성화 스트레스에 민감하게 작용하는데 본 연구에서도 사육수의 pH 변화에 따라 항산화 효소작용이 유의하게 변화하였다. 이러한 결과로 복해마(H. kuda)에 있어서 산성화 노출을 통한 생리학적 스트레스가 항산화 반응 및 체내 성분과 성장을 저해하는 것으로 여겨진다. The rising concentration of atmospheric carbon dioxide is causing ocean acidification and global warming. The seahorse is an important species in marine ecosystems and fishery markets, however, their populations have recently decreased due to ocean acidification. As a result, we examined changes in the physiological responses of the spotted seahorse Hippocampus kuda when it was exposed to acidic sea water (pH 6.0, 6.5, and 7.0) and normal seawater (pH 8.0 as the control) over a period of 15 days. As the pH decreased, the seahorses’ body weight and length also decreased. Components in body of ash, the crude lipids and crude proteins also differed significantly with changes in pH, due to stress caused by the seahorses’ exposure to the acidic water conditions. The superoxide dismutase levels were significantly lower in the pH 6.0 and 6.5 groups than they were in the pH 7.0 and pH 8.0 groups. However, the catalase and glutathione levels were significantly higher in the acidic sea water groups. We suggest that decreasing the pH level of rearing water induces a stress response in H. kuda, damaging their ability to maintain their homeostasis and energy metabolism. Antioxidant enzymes are generally sensitive to acidic stress; in this study, the antioxidant activity was significantly affected by the pH level of the rearing water. These results indicate that physiological stress, induced by exposure to acidification, induces an antioxidant reaction, which can reduce general components in the body and the growth of H. kuda.
제주도 양식 넙치(Paralichthys olivaceus)에서 분리한 병원균 3종에 대한 Mixed Oxidant 및 차아염소산나트륨 살균효과
박천만 ( Cheonman Park ),김기혁 ( Ki-hyuk Kim ),문혜나 ( Hye-na Moon ),여인규 ( In-kyu Yeo ) 한국수산과학회(구 한국수산학회) 2018 한국수산과학회지 Vol.51 No.4
Marine pathogenic bacteria, such as Streptococcus parauberis, Edwardsiella tarda and Vibrio harveyi, can cause lethal infections in farmed fish, ozone and antibiotics, are employed to sterilize waters used for rearing fish to mitigate this threat. The most widely used method is treatment with sodium hypochlorite solution. However, the maintenance of a constant concentration of chlorine in rearing waters can be difficult. We investigated the potential of a mixed oxidant (MO) solution generated by electrolysis of sea water to improve water quality. We measured the survival rates of fish pathogenic bacteria exposed to different concentrations of MO (0.5, 1.0, 1.5 and 2.0 MO) and sodium hypochlorite (0.5, 1.0, 1.5 and 2.0 ppm) for various lengths of time (0, 5, 10, 15, 20, 25 and 30 min). We found a time-dependent decrease in the survival rates of the tested pathogenic microorganisms. The sterilization effect of the MO solution on pathogenic organisms was greater than that of sodium hypochlorite for gram-negative and gram-positive bacteria. We conclude that MO solution produced by electrolysis could be used to maintain a constant chlorine concentration in aquaculture systems.
온도에 의해 유도된 2단계 배양전략을 통한 미세조류 Chlorella vulgaris와 Dunaliella salina의 지질과 탄수화물의 축적량 변화
이연지 ( Yeon-ji Lee ),이치헌 ( Chi-heon Lee ),조기철 ( Kichul Cho ),문혜나 ( Hye-na Moon ),남궁진 ( Jin Namgung ),김기혁 ( Ki-hyuk Kim ),임병진 ( Byung-jin Lim ),김대경 ( Daekyung Kim ),여인규 ( In-kyu Yeo ) 한국수산과학회(구 한국수산학회) 2017 한국수산과학회지 Vol.50 No.1
The aim of this study was to evaluate a temperature-induced two-stage cultivation (TTC) strategy for the regulation of lipid and carbohydrate production by two microalgae, Chlorella vulgaris and Dunaliella salina, for biofuel production. The microalgae were grown under several temperature conditions (15, 25, 35, and 45°C) and optimal growth was observed at 25°C for both microalgae. To test the TTC, aseptically cultured microalgae were incubated under optimal conditions (25°C) for 20 days, and then divided into four aliquots that were incubated at 15, 25, 35, and 45°C for 5 days. Similar but somewhat decreased growth rates were observed at the non-optimal temperatures (15, 35, and 45°C). In addition, while total lipid accumulation increased in a temperature-dependent manner in both microalgae, total carbohydrate increased with temperature in C. vulgaris but decreased in D. salina. However, for lipid and carbohydrate production, while the highest lipid productions of C. vulgaris and D. salina were observed at 25°C and 35°C, respectively, the highest total carbohydrate productions of C. vulgaris and D. salina were obtained at 15°C and 25°C, respectively. These results suggest that the TTC strategy may be easily and efficiently applied to bioprocessing for biofuel production.