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신갈나무 약산 전처리 공정 중 반응표면분석법을 이용한 액상 가수분해물의 furfural 수율 탐색
류가희 ( Ga Hee Ryu ),정한섭 ( Han Seob Jeong ),장수경 ( Soo Kyeong Jang ),홍창영 ( Chang Young Hong ),최준원 ( Joon Weon Choi ),최인규 ( In Gyu Choi ) 한국목재공학회 2016 목재공학 Vol.44 No.1
In this study, furfural, which is one of the value-added chemicals, was produced from the hydrolyzate of Quercus mongolica using dilute acid pretreatment, and the optimal pretreatment condition was determined by Response Surface Methodology (RSM) to obtain high yield of furfural. Based on Central Composite Design, the pretreatment experiment was designed with parameters such as reaction temperature (X1), acid concentration (X2), and reaction time (X3) as independent variables, while dependent variable was furfural concentration (Y), and furfural yield (Z) was shown as percentage of Y per a dry weight basis. According to results of RSM, it was confirmed that reaction temperature (X1) was the most influence factor and reaction temperature (X1)-acid concentration (X2) was the most significant interaction factor on furfural yield. Also, the optimal condition for the highest furfural yield was predicted at reaction temperature of 184℃, acid concentration of 1.17%, and reaction time of 5 min by RSM, and expected maximum yield of furfural was 6.37%. Experimentally, the maximum yield of furfural produced at above optimal condition was 6.21%, and it was considerably similar with the predicted value, and therefore the model for furfural production from the hydrolyzate of Quercus mongolica during dilute acid pretreatment could be built using RSM.
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DNA 데이터베이스의 좌위 확장에 의한 해결 사례 보고
정주연(Jung, Ju Yeon),류가희(Ryu, Ga Hee),김주영(Kim, Joo Young),안으리(Ahn, Eu Ree),문서현(Moon, Seo Hyun),강필원(Kang, Pil Won),최동호(Choi, Dong Ho) 경찰대학 경찰학연구편집위원회 2020 경찰학연구 Vol.20 No.4
2010년 7월 제정된 「디엔에이신원확인정보의 이용 및 보호에 관한 법률」에 의해 구속피의자, 수형인 및 사건현장에서 확보된 DNA 신원확인정보 관리 목적의 데이터베이스가 구축되었다. 미국 FBI(Federal Bureau of Investigation)의 CODIS(Combined DNA Index System) 중심 좌위(Core loci)가 확장됨에 따라 대한민국의 DNA 데이터베이스 좌위도 2018년부터 13개에서 20개로 확장되었다. 본 논문에서는 2018년 1년간 의뢰된 구속피의자 DNA 프로필이 좌위 확장에 의해 DNA 데이터베이스 수록 기준에 도달한 현장증거물과 일치함으로써 사건 해결에 기여한 사례를 보고하고자 한다. 2018년 1년간 수록된 구속피의자 4,947건 중에 28건이 이러한 경우에 해당하였고, 이와 일치된 현장증거물의 약 86%가 미량(Low Copy Number, LCN) 시료인 접촉증거물로부터 검출된 것으로 분석되었다. 지속적으로 접촉증거물의 의뢰가 증가함에 따라 좌위 확장에 의한 해결 사례가 증가할 것으로 판단되었으며, 혼합된 DNA 프로필로부터 1인의 DNA 프로필 분리 가능성도 증가됨으로써 성범죄 등의 강력범죄 해결에도 도움이 될 것으로 사료되었다. Under the ‘Act on the Use and Protection of DNA Identification Information’ enacted in July 2010, a DNA identification database was constructed for the purpose of managing DNA information obtained from detained suspects (arrestees), prisoners, and crime scenes. As the Federal Bureau of Investigation (FBI) Combined DNA Index System (CODIS) core loci has expanded, the Korea DNA identification database loci have also expanded, from 13 to 20 in 2018. Here, we report the information that contributed to case resolution by matching arrestees to the DNA profile obtained from crime scene evidence, and the criteria was reached by loci expansion. Of the 4,947 arrestees whose DNA was uploaded in 2018, 28 cases were included. In addition, we found that approximately 86% of the crime scene evidence that corresponded to these cases were extracted from touched-evidence, which are low copy number (LCN) samples. As the number of requests for touched-evidence continues to increase, the number of cases that are resolved by loci expansion are expected to increase steadily as well. Furthermore, as the possibility of separating a single DNA profile from a mixture also increases, loci expansion will have additional utility in resolving violent crimes, including sex crimes, as well.
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약산 전처리 조건에 따른 소경 신갈나무 유래 푸르푸랄 및 레불린산의 함량 변화
장수경 ( Soo Kyeong Jang ),정한섭 ( Han Seob Jeong ),홍창영 ( Chang Young Hong ),김호용 ( Ho Yong Kim ),류가희 ( Ga Hee Ryu ),여환명 ( Hwanmyeong Yeo ),최준원 ( Joon Won Choi ),최인규 ( In Gyu Choi ) 한국목재공학회 2015 목재공학 Vol.43 No.6
In this study, dilute acid pretreatment was operated using small-diameter Quercus mongolica for evaluating the yield change of furfural and levulinic acid depending on pretreatment factors. The dilute acid pretreatment was conducted depending on reaction temperature (140-180℃), reaction time (10-30 min), and sulfuric acid concentration (0-2%, w/w). Then, glucose, XMG (xylose + mannose + galactose), furfural, and levulinic acid contents in the liquid hydrolyzate were measured and analyzed after pretreatment. Glucose content increased to 16.02% as reaction temperature, reaction time, and sulfuric acid concentration increased, but it decreased at the sulfuric acid concentration of 2% (reaction temperature: > 170℃, reaction time: > 20 min). On the other hand, reaction temperature had a strong influenced on XMG content, and XMG content decreased to 1.63% through increasing of reaction temperature and sulfuric acid concentration, but XMG content was less affected by changes of reaction time. Furfural content increased with the increase of reaction temperature, reaction time, and sulfuric acid concentration, and maximum furfural content was 7.61% (reaction temperature: 180℃, reaction time: 20 min, sulfuric acid concentration: 1%) based on a weight of raw material, while furfural content was dropped in more severe condition than in maximum furfural content condition. Levulinic acid content also increased with higher reaction temperature, reaction time, and sulfuric acid concentration. Especially, the sharp increase of levulinic acid content was observed above 170℃, and maximum levulinic acid content was 10.98% (reaction temperature: 180℃, reaction time: 30 min, sulfuric acid concentration: 2%). However, less than 1% of furfural and levulinic acid content was obtained in non-acidic catalyst condition that in whole conditions of reaction temperature and reaction time.
ScienceON
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