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Citric Acid Production from Concentrated Milk-wastewater by Aspergillus niger
김세권,노호석,변희국,전유진,Kim, Se-Kwon,Roh, Ho-Seok,Byun, Hee-Guk,Jeon, You-Jin 한국생명과학회 1996 생명과학회지 Vol.6 No.1
The possible use of milk-wastewater as a fermentation media for the production of citric acid by Aspergillus niger has been investigated. The addition of Mn$^{2+}$ , Fe$^{2+}$ and Cu$^{2+}$ to the medium promoted citric acid production while only Mg$^{2+}$ decreased citric acid production. The concentrations of citric acid produced were marked up to 7.2g/l and 16.5g/l in a batch bioreactor by Aspergillus niger ATCC 9142 with 50g/l and 100g/l of reducing sugar concentration in milk-wastewater, respectively. A mathematical model was developed and simulating the predictability of cell growth, citric acid production and substrate consumption rate, and gave good agreement results with experimental data. 우유공장에서 배출되는 폐수를 Aspergillus niger ATCC 9142의 발효기질로 하여 회분식발효기에서 구연산의 생산에 관하여 연구하였다. 구연산의 생산성을 높이기 위하여 각종 금속이온, Mn$^{2+}$, Fe$^{2+}$, Cu$^{2+}$ 및 Mg$^{2+}$ 등을 첨가하여 발효시킨 결과, Mn$^{2+}$, Fe$^{2+}$, Cu$^{2+}$의 첨가시 구연산의 생산이 증가하였으나, Mg$^{2+}$를 첨가한 경우는 오히려 감소하였다. 우유폐수의 환원당 농도를 각가 50g/l 및 100g/l로 농축하여 Aspergillus niger ATCC 9142를 이용하여 생산된 구연산은 7.2g/l 및 16.5g/l 이상이었다. 실험적인 결과에 의한 세포의 성장, 구연산의 생산 및 기질의 소모속도는 수확적인 모델링에 의해 예측한 경우와 잘 일치하고 있음을 알 수 있었다.
이응호,이원희,김세권,변희국,최광덕,노호석 한국수산학회 1993 한국수산과학회지 Vol.26 No.2
Collagenase existed in the internal organs of filefish Novoden modestrus was isolated with ammonium sulfate and was purified by ion exchange column chromatography with DEAE-Sephadex A-50 and gel filtration with Sephadex G-150. The activity of the purified enzyme was increased 92.4 folds than that of the crude one and the yield of the purified one was 10.9%. The optimum conditions showing the maximum activity of the crude enzyme to digest insoluble collagen(Type I) were 55℃ and pH 8.0, while those showing the maximum activity of the purified one were 55℃ and pH 7.75. However, the use of the crude enzyme for skinning of filefish was more profitable because the yield was 800 folds higher than that of the purified one and the cost was also able to economy. When hydrolysis for skinning of filefish was conducted with 0.3%(w/w) crude collagenase at 50℃ and pH 8.0 for 3hrs, there was some problem to cause a damage on muscle of the fish by heat. To solve such problem for the skinning, the hydrolysis at 18℃ for 4hrs with 0.3%(w/w) crude enzyme after pretreated with 0.5M acetic acid for 10 min provided a good result for skinning of filefish.