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- 저자 : 김효비(Hyobi Kim) (검색결과 3 건)
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국내 흑염소에서의 소결핵, 브루셀라, Q fever 유병률 연구
김효비,김성준,김기나,김별,장병준,최농훈,Kim, Hyobi,Kim, Seongjoon,Kim, Kina,Kim, Byeol,Chang, Byungjoon,Choe, Nong-Hoon 대한수의학회 2016 大韓獸醫學會誌 Vol.56 No.4
As the meat of black goats has become popular as a healthy food, domestic goat meat-related industries are steadily growing. However, previous studies are scarce of informations about the zoonotic disease originated from the black goat in Korea. In this study, we investigated Korean black goat's infectious diseases representing bovine tuberculosis, brucellosis, and Q fever. One hundred and eighty samples were collected from a local slaughter house located in Jeollanam-do. Three typical zoonotic diseases were separately examined by carrying out enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Histopathological test was additionally performed in tuberculosis. In case of tuberculosis, results of the PCR and histopathological test were negative but the ELISA results were positive in eight samples. In case of brucellosis, one out of the total samples was shown to be positive in the ELISA and none in the PCR. In case of Q fever, there were forty one positive in the ELISA and twenty positive in the real-time PCR. Those results indicate that the Korean black goat could be a natural reservoir in the possible chain-infections among human, cows and goats. Thus, further study needs in order to improve productivity as well as to prevent the zoonosis spreading and circulation of other livestock with the black goat in this country.
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LC-MS/MS를 이용한 닭 간과 신장 중 곰팡이 독소 6종 동시분석법 개발
김수희(Soohee Kim),김광남(Kwang-Nam Kim),김효비(Hyobi Kim),송재영(Jae-Young Song),박성원(Sung-Won Park) 한국가금학회 2016 韓國家禽學會誌 Vol.43 No.2
본 연구에서는 곰팡이 독소에 대한 노출을 확인하기 위한 목적으로 닭의 간, 신장 조직에서 곰팡이 독소 분석법을 확립하였다. 곰팡이 독소의 경우 닭에서 독성이 강하며, 본 실험에서는 가축의 사료에서 주로 확인되는 곰팡이 독소 6종(아플라톡신 B1․M1, 오크라톡신 A, 푸모니신 B1, 데옥시니발레놀, 제랄레논)을 선별하여 추출, 정제조건을 확립하고 LC-MS/MS를 이용하여 분석하였다. 확립된 분석조건에서 검량선은 R2값이 0.99 이상으로 우수한 직선성을 나타내었다. QUECHERS법을 응용하여 닭 간, 신장 시료에서 곰팡이 독소를 추출, 정제하여 분석하였을 때 곰팡이 독소 4종(아플라톡신 B1, 오크라톡신 A, 데옥시니발레놀, 제랄레논)의 평균회수율은 80.94∼98.10%이고, 상대표준편차도 14% 미만으로 조사되어 높은 정확도와 정밀도를 확인할 수 있었다. 검량선에 근거하였을 때 곰팡이 독소 6종에 대하여 닭 간 시료의 경우 검출한계는 7.6∼145.79 μg/kg, 정량한계는 23.04∼441.78 μg/kg이었다. 닭 신장의 경우 검출한계는 6.07∼197.20 μg/kg, 정량한계는 18.40∼597.59 μg/kg으로 나타났다. 본 연구의 결과 LC-MS/MS를 이용하여 닭의 간, 신장에서 곰팡이 독소 6종 동시 분석법을 확립하였으며, 이는 생체시료에서 효율적인 곰팡이 독소 동시 분석법으로 활용이 가능할 것으로 기대된다. Mycotoxins are secondary metabolites produced by molds, such as Aspergillus, Fusarium and Penicillium, that have adverse effects on animals and humans. Aflatoxin, ochratoxin, zearalenone, fumonisin and deoxynivalenol are the mycotoxins of greatest agro-economic importance and cause acute disease called mycotoxicoses. Mycotoxicosis in poultry birds results in decreased meat/egg production, immunosuppressant, and hepatotoxicosis. Some of toxins or their metabolites may be retained in animal or human tissues and induce health problems. This study was designed to develop a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection and quantification of mycotoxins, such as aflatoxin B1, aflatoxin M1, ochratoxin A, zearalenone, fumonisin B and deoxynivalenol, in chicken liver and kidney tissues. The mycotoxins were extracted and purified using modified QUECHERS methods, separated by LC and detected by an electrospray ionisation interface (ESI) and tandem MS. Good precision and linearity were observed for most of six mycotoxins. The recovery test for each mycotoxin in liver and kidney tissues mostly indicated good average recovery rates between 80.94% and 98.10% and the coefficient of variation mostly under 13.78%, except for aflatoxin M1 and fumonisin B1. The limit of detection (LOD) for six mycotoxins was 7.6∼145.79 μg/kg in liver tissues and 6.07∼197.20 μg/kg in kidney tissues. The quantification limits (LOQ) for 6 mycotoxins were in the range 23.04∼441.78 μg/kg in liver tissues and 18.40∼597.59μg/kg in kidney tissues, respectively. The developed multi-mycotoxin method in this study permits simultaneous, simple, and rapid determination of several co-existing mycotoxins in chicken liver and kidney tissues.
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장양호,이수진,김효비,이정학,이만호,길혜경,최농훈,Jang, Yangho,Lee, Soojin,Kim, Hyobi,Lee, Jeonghak,Lee, Manho,Gil, Hyekyoung,Choe, Nonghoon 대한수의학회 2011 大韓獸醫學會誌 Vol.51 No.1
In Korea, groundwater is main water source in livestock farms. Most dairy and cattle farms have constructed their own wells for human drinking and livestock farming. However, these private residential wells have not been controlled by government and also there was scant study about livestock drinking water quality. Therefore this study was to monitor of the livestock farms' groundwater quality in Korea. Water samples were collected at 123 dairy and cattle farms and were analysed forty six substances with quality standard for drinking water approved by the Minister of Environment. Seventy eight (63.4%) of 123 samples failed to drinking water stand a test. The most frequent contaminants were nitrate-nitrogen and microbial. 22.8% (n=28) of samples showed nitrate-N concentration of higher than 10 mg/L meant that can't be used drinking water for human and the Nitrate-N concentration analysed in the range of 0.2 to 61.2 mg/L. All of 78 failed to drinking samples had microbial problems, especially 5.7% (n=7) of samples indicated water could be contaminated by feces. Other contaminants detected were zinc and evaporation residue. Especially detected zinc concentration (32 mg/L) was about ten times higher than standard of zinc (3 mg/L). Regression analysis indicated that groundwater pH did not influence to nitrate-N concentration but the hardness and chloride could affect to nitrate-N concentration in the groundwater. Most livestock farms were adjacent to crop farmland in Korea. This could cause contamination of groundwater with nitrate-N and pesticide that could accumulate livestock product. Moreover Heavy metal such as zinc and copper could be released from a corrosive plated water pipe in livestock farm. Put together, Korea livestock system is indoor, not pasture-based, hence livestock could be exposed to potential contaminated water consistently. Therefore on the basis of these data, appropriate livestock drinking water quality standards should be prepared to keep livestock healthy and their product safe. Further, livestock drinking water quality should be monitored continuously in suitable livestock drinking water standards.
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