소나무의 몇가지 다형적 동위효소의 유전분석 (Ⅰ) - Glutamate - Oxalate Transaminase 와 Leucine Aminopeptidase 의 유전과 동위효소 유전자좌 간의 연관관계 -

김진수,홍용표 ( Zin Suh Kim,Yong Pyo Hong ) 한국산림과학회 1982 한국산림과학회지 Vol.58 No.1

Megagametophyte and embryo tissue of Pinus densiflora were subjected to study the inheritance of glutamate-oxalate transaminase(GOT) and leucien aminopeptidase(LAP), and linkage relationship among isozyme loci coding both enzymes by starch gel zone-electrophoresis. Four zones of activity were observed for GOT. No variation was found in the fastest migrating zone (GOT-A), Electrophoretic phenotypes of the other two zones (GOT-B and GOT-C) showed 1:1 segregation ration, suggesting that each zone is controlled by a single locus. Foru and three alleles were identified at both loci respectively. The isozyme pattern of the fourth zone(GOT-D), migrated chthodally, coincided precisely with that of GOT-C, Whether the two zones are controlled by the smae locus or by two tightly linked loci remained unknown. In all three variant GOT zones, heterozygoes embryos produced triple band patterns, indicating that GOT isozyme in Pinus densiflora is a dimer. Two zones of activity stained for LAP were found. The segregation of the two zones (LAP-A and LAP-B) suggested that tow loci control each of both isozymes. Two and three alleles were identified at both loci. GOT-B and LAP-B were found to be tightly linked, showing an average recombination frequency of 12.5 percent. Slight deviation from independent assortment was observed between GOT-B and GOT-C, with recombination frequency of 41 percent.

No Trace of Introduced cpDNA of Pinus thunbergii in Pinus densiflor for. erecta Postulated as an Introgressive Hybrid between Pinus densiflora and Pinus Thunbergii

홍용표,김규식,노의래,신은명,김진수,Hong, Yong-Pyo,Kim, Kyu-Sik,Noh, Eui-Rae,Shin, Eun-Myeong,Kim, Zin-Suh Korean Society of Forest Science 1998 한국산림과학회지 Vol.87 No.4

Portions of chloroplast genes(psbD and rbcL) were amplified from Pinus thunbergii(Japanese black pine : black pine) and Pinus densiflora(Japanese red pine : red pine) by PCR and digested by a restriction enzyme, HaeIII, respectively. Two species specific cpDNA markers were identified. With the observed cpDNA markers, paternal inheritance of cpDNA in pine hybrids was verified in an artificial hybrid family between black pine(Chollanam 37) and red pine(Chungchongbuk 3). On the basis of paternal inheritance of chloroplast genome in a hybrid, 2 portions of cpDNA amplified from 115 individuals of Pinus densiflora for. erecta were screened to detect any traces of black pine specific cpDNA markers in P. densiflora for. erecta which has been postulated as an introgressive hybrid between red pine and black pine(Hyun el al., 1967). All the analyzed individuals of Pinus densiflora for. erects revealed the identical profiles of HaeIII digested psbD and rbcL genes to red pine. This result suggests that there is no introduced chloroplast genome of black pine in Pinus densiflora for. erecta and that there is no concrete evidence of treating P. densiflora for, erecta as an introgressive hybrid between red pine(♀) and black pine(♂). 소나무와 해송으로부터 엽록체상의 두 유전자 psbD와 rbcL를 PCR에 의해 증폭한 후 제한효소 HaeIII를 사용해서 절단했다. 두 개의 종 특이적 엽록체 DNA 단편이 확인되었고, 이 두 개의 표지자를 이용하여 소나무(충북3호)와 해송(남난37호)의 인공교잡 가계로부터 엽록체 DNA의 부계 유전양식이 확인되었다. 인공교잡 가계에 있어서 엽록체 DNA의 부계 유전양식을 근거로 해송으로부터 소나무로의 이입교잡에 의해 생겨났다는 가설(현신규 등, 1967)이 지배적인 금강송 115개체로부터 이입교잡에 의해 유입되어진 흔적을 구명하기 위하여 해송 특이 엽록체 DNA의 존재 여부를 검색하였다. 분석에 사용된 금강송 전 개체에서 소나무에서 관찰된 엽록체 DNA(psbD와 rbeL)의 절편 분획 양상과 동일한 절편 분획 양상이 확인되었다. 본 실험의 결과로부터는, 금강송에는 해송으로부터 유입된 엽록체 게놈의 흔적을 찾아 볼 수 없었으며, 따라서 금강송을 소나무(♀)약 해송(♂)의 이입교잡종이이라고 간주할만한 확고한 증거를 제시할 수 없었다.

소나무와 곰솔간 이입교잡종으로 추정되어온 금강송에 있어서 곰솔 cpDNA 의 부재

홍용표,김규식,노의래,신은명,김진수 ( Yong Pyo Hong,Kyu Sik Kim,Eui Rae Noh,Eun Myeong Shin,Zin Suh Kim ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.4

Portions of chloroplast genes(psbD and rbcL) were amplified from Pinus thunbergii(Japanese black pine : black pine) and Pinus densiflora(Japanese red pine : red pine) by PCR and digested by a restriction enzyme, HaeIII, respectively. Two species specific cpDNA markers were identified. With the observed cpDNA markers, paternal inheritance of cpDNA in pine hybrids was verified in an artificial hybrid family between black pine(Chollanam 37) and red pine(Chungchongbuk 3). On the basis of paternal inheritance of chloroplast genome in a hybrid. 2 portions of cpDNA amplified from 115 individuals of Pinus densiflora for. erecta were screened to detect any traces of black pine specific cpDNA markers in P densiflora for. erecta which has been postulated as an introgressive hybrid between red pine and black pine(Hyun el al., 1967). All the analyzed individuals of Pinus densiflora for, erects revealed the identical profiles of HaeIII digested psbD and rbcL genes to red pine. This result suggests that there is no introduced chloroplast genome of black pine in Pinus densiflora for. erecta and that there is no concrete evidence of treating P. densiflora for, erecta as an introgressive hybrid between red pine(♀) and black pine(♂).

계층적 센서 네트워크에서 균등한 에너지 소비를 위한 클러스터링 기법에 관한 연구

김요섭(Kim, Yo-Sup),홍영표(Hong, Yeong-Pyo),조영일(Cho, Young-Il),김진수(Kim, Jin-Su),은종원(Eun, Jong-Won),이종용(Lee, Jong-Yong),이상훈(Lee, Sang-Hun) 한국산학기술학회 2010 한국산학기술학회논문지 Vol.11 No.9

에너지 효율성이 중요한 무선 센서 네트워크에서 클러스터링 기술은 클러스터 헤드 노드가 클러스터 멤버 노드의 데이터를 병합하여 싱크노드로 전송함으로써 센서노드와 싱크노드 사이의 통신 횟수를 줄여 에너지 효율을 얻는다. 본 논문에서는 분산형 클러스터링 라우팅 기법 중 대표적 프로토콜인 LEACH(Low Energy Adaptive Clustering Hierarchy)와 HEED(Hybrid, Energy-Efficient Distributed Clustering Approach)의 클러스링 기법에 대하여 분 석하고 이를 토대로 데드노드의 최대 지연 발생과 네트워크 라이프 타임을 증가하기 위한 새로운 에너지 효율적 클 러스터링 기법을 제안한다. 제안 방식은 클러스터 헤드가 각 멤버노드의 잔여에너지 정보와 싱크노드와의 위치 정보 를 기반으로 최적의 전송 효율을 위한 노드를 선출 하고, 선출된 노드는 이후 데이터 전송과정에서 클러스터 헤드로 부터 데이터를 전송받아 싱크노드로 전송하는 방식으로, 네트워크를 형성하는 개별 노드의 에너지 소비를 균등하게 하여 네트워크의 전체 수명을 증가시키는데 본 연구의 목적이 있다. 제안한 방식의 성능을 검증하기 위해 시뮬레이션 을 통해 기존의 클러스터링 기법과 비교 분석하였다. 그 결과, 기존의 기법에 비해 네트워크의 생명주기가 약 5~10% 향상되는 것을 확인 할 수 있었다. The Clustering technology of Energy efficiency wireless sensor network gets the energy efficiency by reducing the number of communication between sensor nodes and sink node. In this paper, First analyzed on the clustering technique of the distributed clustering protocol routing scheme LEACH (Low Energy Adaptive Clustering Hierarchy) and HEED (Hybrid, Energy-Efficient Distributed Clustering Approach), and based on this, new energy-efficient clustering technique is proposed for the cause the maximum delay of dead nodes and to increase the lifetime of the network. In the proposed method, the cluster head is elect the optimal efficiency node based on the residual energy information of each member node and located information between sink node and cluster node, and elected a node in the cluster head since the data transfer process from the data been sent to the sink node to form a network by sending the energy consumption of individual nodes evenly to increase the network's entire life is the purpose of this study. To verify the performance of the proposed method through simulation and compared with existing clustering techniques. As a result, compared to the existing method of the network life cycle is approximately 5-10% improvement could be confirmed.

캘리포니아 폐구와 소나무류의 엽록체 DNA rbcL 과 atpB 유전자 사이에 존재하는 염기 서열

김진수,홍용표 한국유전학회 1992 Genes & Genomics Vol.14 No.2

The intergenic sequence between rbcL and atpB genes of chloroplast DNA were sequenced from five individual trees, one individual each of Monterey pine (Pinus radiata), knobcone pine (Pinus attenuata), and three geographic groups of bishop pine (Pinus muricata). No variation was observed among the 667 bp intergenic sequences of five individuals which had been directly sequenced with the PCR(polymerase chain reaction)-amplified DNA. Alignment of the upstream sequences (251 bp) of the rbcL gene with those of Douglas-fir showed about 88.4% similarity.