뱀장어 아기미 울혈증에 관한 연구

김영길(Young Gill Kim),최민순(Min Soon Choi),박성우(Sung Woo Park),이근광(Keun Kwang Lee) 한국어병학회 1994 한국어병학회지 Vol.7 No.2

In order to elucidate the outbreak mechanisms of a new disease which is characterized by an intense congestion in central venus sinuses(CVS) of gill filaments in cultured eel. these experiments were carried out; epidemically surveyed on the cultured eel farms in the vicinity of Kunsan city and experimentaliy outbreaked the disease in the stressful condition such as thermal and handling shock and innoculated the supernatant from the homogenate of naturally severe congested gill into eels and onto the monolayer of the CHSE-214. Although the frequency of congestion in eels of B, C, D and E farms were higher than in eels of A farms, the water qualities (stocked and cultured water) among farms were not a great difference. In eels of B, C, D and E farms, the values of haematocrit (Ht), haemoglobin (Hb), total protein (Tp), albumin (Alb), glucose (Glu), magnesium (Mg) were lower and the values of calcium(Ca), methemoglobin(Met-Hb), glutamic pyruvic transminase (GPT), glutamic oxalacetic transminase (GOT) higher than in eels of A farms. These valules have not related to the frequency of congestion. An intensive congestion and dilataton in CVS of gill filaments in experimentally handling-stressed eels produced similar histopathological changes to those observed in the spontaneously diseased eel, but not in eels experimentally injected with filtering contents. The cytopathic effect on the CHSE-214 was not observed. In stressed eels the congestion of gill was increased in relation to either the decrease ranges of water temperature or the incerase in accllimated times. And increase in Ht, Met-Hb, Alb, Glu, GOT and GPT and decrease in Mg, Hb and Tp were found, which had a close relationship to congestion of gill. Cortisol were increased according to the decrease ranges in acclimated water temperature. From these results, decrease in water temperature during selection placed eels upon the stressed condition, made increase in ionic strength in blood stream, and CVS was dilatated owing to the increased blood inflow.

조개류(類)에 기생(寄生)하는 흡충류(吸蟲類)에 관(關)한 연구(硏究) V. 동죽, 가무락조개 및 맛조개에서 검출(檢出)되는 극구흡충류(棘口吸蟲類)의 유충(幼蟲)에 대(對)하여

김영길,Kim, Young-Gill 한국어병학회 1988 한국어병학회지 Vol.1 No.1

해산이매패(海産二枚貝)의 흡충류감염종(吸蟲類感染種)과 패류(貝類)의 병해(病害)를 조사할 목적으로 서해안(西海岸) 금강하구(錦江河口) 내초도산(內草島産) 동죽, 가무락 조개 및 맛조개에서 극구흡충과(棘口吸蟲科)의 피낭유충(被囊幼蟲)을 검출(檢出)하였고, 맛조개에서 검출된 피양유충(被襄幼蟲)을 괭이갈매기에 감염(感染)시켜 16일(日)만에 성충(成蟲)을 얻었다. 1. 동죽 및 가무락조개에서는 Himasthla kusasigi의 피낭유충을 검출(檢出)하였으며, 기생율(寄生率)은 각각 70.4%, 85.7% 이었다. 2. 조직부위별(組織部位別) Himasthla kusasigi의 기생율(寄生率)은 족부(足部) 40.4%, 77.3%, 아가미 17.0%, 12.4%, 외투막 12.6%, 10.3% 순으로 기생(寄生)되었다. 3. 맛조개에서 Acanthoparyphium속(屬) 흡충(吸蟲)의 피낭유충(被囊幼蟲)을 검출(檢出)하였으며, 기생율(寄生率)은 63%, 조직부위별 기생율은 foot, mantle, gill 순(順)으로 기생(寄生) 되었다. 4. 맛조개에서 검출(檢出)된 피낭유충(幼蟲)을 괭이갈매기에 감염(感染)시켜 16일만에 성충(成蟲)을 분리하여 충체(蟲體)의 내부(內部)구조를 조사한바 Acanthoparyphium tyosenensis Yamaguti, 1938로 동정(同定) 되었다. 5. 동죽 및 가무락조개는 Himathla kusasigi의 새로운 중간숙주(中間宿主)로 추가되며, 본인(本人)(1968)등(等)이 개량조개에서 검출보고(檢出報告)한 Echinostoma속 흡충(吸蟲)은 Acanthoparyphium tyosenensis흡충(吸蟲)의 유충(幼蟲)임이 밝혀졌다. An experiment was carried out in order to reveal the infection species of trematode and the infection demage in the marine bivalves. Metacercaria of Echinostomatidae were found in Mactra veneriformis, Cyclina sinensis and Solen strictus in the vicinity sea of Naecho-do, the estuary of the Keum River in the western coast of Korea. The metacercaria detected from Solen strictus were fed by Larus crassiostris, at 16 days after infection, the adult worm could be seceded. The metacercaria of Himasthla kusasigi were detected from Mactra veneriformis and Cyclina sinensis, their infection rates were 70.4% and 85.7% respectively. The partial infection rates with respect to the body parts were 40.4% and 77.3% in the foot, 17.0% and 12.4% in the gill, 12.6% and 10.3% in the mantle in order. The metacercaria of Acanthoparyphium were found from Solen strictus. The total infection rate was 63% and the partial infection rate was 63% and the partial infection rate was shown foot, mantle and gill in order. The adult worm ceded from Larus crassiostris was indentified as Acanthoparyphium tyosenensis Yamaguti, 1938 from view point of its physical characteristics. Therefore, Mactra veneriformis, Cyclina sinensis would be added as new intermediate hosts, and Echoinostoma decteded from Mactra sulcataria which was studied by author(1969) was revealed as larvae of Acanthoparyphium tyosenensis.

Acanthoparyphium tyosenense Yamaguti, 1939의 생활사에 관한 연구

김영길,유지은,KIM Young-Gill,YU Ji-Eun 한국수산과학회 2001 한국수산과학회지 Vol.34 No.6

Cercaria yamagutii Ito, 1957 (C. yamagutii) was found in Lunatia fortuni (L. fortuni) and Neverita didyma (N. didyma) collected from the tideland of Sim-po located at the estuary of the Mankyong River, Chonbuk. It was finally confirmed that the parasite is Acanthoparyphium tyosenense Yamaguti, 1939 (A. tyosenense) and its life history was clarified in this study. Mactra veneriformis (M. veneriformis) was artificially infected with C. yamagutii isolated from L. fortuni and N. didyma. It began to intrude into M. veneriformis through the inhalent canal. Five hours after infection, the tails of the cercaria began to be separated from the main body and the cercaria started to form cysts. Mature cysts were formed 340 hours (14 days) after infection. The cysts were $300\sim360{\mu}m$ in diameter and the encysted metacercarias were $790\sim800\times300\sim310{\mu}m$ in size. The metacercarias were administered orally to Larus crassiostris (L. crassiostris), and adult worms of $84.5\sim112.5\times55\sim65{\mu}m$ were found full of eggs with $2.20\~3.70$ mm long and $0.40\~0.59$ mm wide after 10 days. In a field study, it was observed that the infection rate of A. tyosenense is $99.5\%$ in M. venerifomis, $76.3\%$ Solen strictus (S. strictus), and $37\%$ Ruditapes philippinarum (R. philippinarum), No difference was found among different host sizes, It was concluded that the first intermediate hosts of A. tyosenense Yamaguti were L. fortuni, N. didyma, Tympanotonus microptera, Cerithidea (Cerithidea) largillierti, Cerithidea (Cerideopsilloa) cingulata, the second intermediate hosts M. venerifomis, S. strictus and R. philippinamn, and the final hosts L. crassiostris and Melanitta fusca stejnegeri. 1999년 3월부터 2000년 3월까지 서해안 만경강 하구역의 심포리 지선 간석지에서 채집한 갯우렁이 (Lunatia fortuni)와 큰구슬우 렁이 (Neverita didyma)에서 Cercaria yamagutii Ito,1957를 검출하고, 실험실에서 이매패류인 동죽 (Mactra venerifomis)에 감염 시켜 피낭유충이 형성됨을 확인하였고, 동 실험에서 형성된 피낭 유충을 괭이갈매기 (Larus crassiostris)에 경구 감염시켜 10일 후 성충을 얻어 Acanthoparyphium tyosenense Yamaguti, 1939임을 확인하여 지금까지 밝혀지지 않았던 이 충의 전 생활사를 밝혀냈다. 제1중간숙주는 해산 복족류인 갯우렁이, 큰구슬우렁이, Tympanotonus microptera와 Cerithidea (Cerithidea) largillierti, Cerithidea (Cerideopsilloa) cingulata이고, 제 2중간숙주는 이매패인 동죽, 개량조개, 맛조개, 바지락이며, 종숙주는 괭이갈매기와 물오리의 일종인 Melaniua fusca stejnegeri이다. 갯우렁이와 큰구슬우렁에서 검출한 Cercaria yamagutii를 실온 $25^{\circ}C$ 전후에서 동죽이 들어있는 수조에 넣은 후 30분 이내에 입수는 피낭의 크기 $300\sim360{\mu}m$, 탈낭유충$790\sim800\times300\sim310{\mu}m$크기로 활발히 신축 운동하는 성숙한 유충으로 발육되었다. 자연산 이매패에서 Acanthopayphium tyosenense의 피낭유충 기생율은 동죽 $99.5\%$, 맛조개 $76.3\%$, 바지락 $37\%$이었고, 피낭유 충의 부위별 기생율은 동죽의 경우 검출된 피낭유충 총 18,064개중 족부 근육 $62.9\%$ (11,368개), 아가미 $23.6\%$ (4,258개), 내장낭$13.5\%$ (2,435개)의 기생율을 나타냈다. 피낭유충은 숙주 종이나 크기와 관계없이 나타났다.

Pantotheic acid 결핍에 의한 동자개치어의 사료성 아기미병

김영길(Young Gill Kim),박성우(Sung Woo Park),이경희(Kyung Hee Lee) 한국어병학회 2000 한국어병학회지 Vol.13 No.1

A new nutritional disease has occurred among the hatchery-reared Korean bullhead fingerlings (Pseudobagrus fulvidraco) in the Chonbuk Province in September 1997. Diseased fish were all dead within 3-7 days, showing sluggish behavior, head up and tail down swimming. Most characteristic clinical signs were anaemia, clubbed and fused gill, skin desquamation. haemorrhage around the mouth and at the base of pectoral fins. Any causative bacteria and parasites were not isolated from the lesions and internal organs of the diseased fish. The hepatosomatic index, red blood cell count, hematocrit, hemoglobin and erythrocytes size of peripheral blood in the diseased fish were remarkably decreased compared with those of normal fish. In the histopathological observations, epithelial hyperplasia of the gill filaments initiated at the base of the gill was pronounced. This symptom was the characteristic appearence of all the diseased fish. A 0.6% saline bath and feeding a pantothenic acid-supplemented diet were conducted to decrease the mortality. Ten days after 0.6% saline bath or 25 days after feeding a pantothenic acid supplemented diet resulted in decreasing in the mortality. Microscopic appereance of the gill from the recovered fish was similar to that of the gill from healthy fish. These results indicate that the disease was caused by deficency of pantothenic acid in their diet and that 0.6% saline bath or supplementation of pantothenic acid in the diet was an effective way to decrease the mortality.

뱀장어 아가미 율혈증에 관한 연구

김영길(Young Gill Kim),최민순(Min Soon Choi),장선일(Seon Il Jang),박관하(Kwan Ha Park),최상훈(Sang Hoon Choi),조정곤(Jeong Gon Cho) 한국어병학회 1995 한국어병학회지 Vol.8 No.2

This experiment was carried out in order to elucidate the effect of NaCl(0.85%) acclimation on congestion symptoms in central venous sinuses(CVS) of gill filaments and on the alteration of blood constituents in cultured eels for 1, 2, 3, 4, 5, 6 and 7days after handling stress. After giving a handling stress on eels, the frequency of gill congestion was about 90%. It gradually decreased to 40% on the 3rd day, then increased to 60% on the 7th day in NaCl(0.85%) acclimation. The values of haematocrit(Ht), methemoglobin(Met-Hb), glucose (GLU), calcium(Ca^(++)), lactic dehydrogenase(LDH), glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase(GOT) were lower than those of the control, whereas magnesium(Mg^(++)) and haemoglobin(Hb) were higher than the control. However total protein(Tp) and albumin (ALB) levels similar to the control. When the fish after giving a handling stress were cultured in NaCl-free water as a control, the frequency of gill congestion and the value of blood components showed a similar pattern to that obtained from the NaCl acclimated experiments. However, in the early period(1-3day) after handling stress, NaCl acclimating slightly helped the natural tendancy of recovery. These results suggest that the frequency of gill congestion is not always reduced over the whole period of NaCl acclimation. However, the acclimation to NaCl solution may be a somewhat useful therapy for the gill congestion in stressed eels.

藍浦海域의 海洋環境 調査

김영길(Kim Young-gill),김용호(Kim Young-ho),홍성근(Hong Sung-Kum),이원우(Lee Won-woo),이정열(Lee Jeong-yeol),김영섭(Kim Young-seup),김종연(Kim Jong-yeon) 군산대학교 수산과학연구소 1986 水産科學硏究 Vol.- No.2

The marine environmental characteristics of the vicinity of Nampo sea area were investigated in order to assess the environmental variation and the ecological influences by the project of reclaimation from February to May, 1986. At flood tide, the water coming from the southwest of Kabampo turned to counter-clockwise and flowed to the in front of Kapbawi with the speed of 0.4-1.2 knot. Whileas, at ebb tide, the current turned to the clockwise in front of Kapbawi and then moved to the off sea with 0.4-1.3 knot. Although the levels of nutrient salts were relatively low, but the SS level was higher compared with that of other areas due to the reclaimation construction. As a whole, the procuctivity of this area is poor state compared with other coastal areas.

뱀장어 아가미 울혈증에 관한 연구 III. NaCl처리가 울혈증 치료에 미치는 영향

최민순,박관하,최상훈,김영길,김종면,조정곤,장선일,Choi, Min-Soon,Park, Kwan-Ha,Choi, Sang-Hoon,Kim, Young-Gill,Kim, Jong-Myeon,Cho, Jeong-Gon,Jang, Seon-Il 한국어병학회 1995 한국어병학회지 Vol.8 No.2

This experiment was carried out in order to elucidate the effect of NaCl(0.85%) acclimation on congestion symptoms in central venous sinuses(CVS) of gill filaments and on the alteration of blood constituents in cultured eels for 1, 2, 3, 4, 5, 6 and 7days after handling stress. After giving a handling stress on eels, the frequency of gill congestion was about 90%. It gradually decreased to 40% on the 3rd day, then increased to 60% on the 7th day in NaCl(0.85%) acclimation. The values of haematocrit(Ht), methemoglobin(Met-Hb), glucose (GLU), calcium($Ca^{++}$), lactic dehydrogenase(LDH), glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase(GOT) were lower than those of the control, whereas magnesium($Mg^{++}$) and haemoglobin(Hb) were higher than the control. However total protein(Tp) and albumin (ALB) levels similar to the control. When the fish after giving a handling stress were cultured in NaCl-free water as a control, the frequency of gill congestion and the value of blood components showed a similar pattern to that obtained from the NaCl acclimated experiments. However, in the early period(1-3day) after handling stress, NaCl acclimating slightly helped the natural tendancy of recovery. These results suggest that the frequency of gill congestion is not always reduced over the whole period of NaCl acclimation. However, the acclimation to NaCl solution may be a somewhat useful therapy for the gill congestion in stressed eels. 뱀장어에 인위적으로 스트레스를 가하여 아가미 울혈증을 발생시킨 후에 NaCl 처리에 따른 CVS의 울혈증의 치료효과 및 혈액성상의 변화에 미치는 영향을 조사하였다. 스트레스 직후에 고빈도의 발병율을 보였던 아가미 및 간의 울혈증은 염처리 후 3일째 까지는 감소되었으나, 4일 이후에는 별 영향이 없었다. 혈액성분중 스트레스 후 증가되었던 Ht, Met-Hb, $Ca^{++}$, GOT, GPT, LDH 및 glucose치 등과 감소되었던 $Mg^{++}$ 및 Hb치 등은 염처리 3일까지는 회복율이 촉진되었으나, 그 이후에는 큰 차이를 보이지 않았다. 그러나 알부민 및 총단백치는 염처리 기간동안 별 변화가 없었다. 이상의 결과는 스트레스에 의해 발병된 아가미 울혈증에 대한 염처리법은 초기 3일까지는 효과가 있으나, 그 이상의 기간에는 전혀 효과적이지 못함을 보여준다.

한국산 메기의 질병에 관한 연구 2 . 비브리오병에 관하여

김영길(Young Gill Kim),이근광(Keun Kwang Lee) 한국어병학회 1993 한국어병학회지 Vol.6 No.1

Two vibrio sp. strains were isolated from disease catfish(silurus asotus). The present isolates were identified as Vibrio ordalii based on their biological and biochemical characteristics; they were positive for acid production from glucose, Iactose, maltose, sucrose and salicine. while negative for arabinose. galactose, inocitol and xylose. They are named KL-1 and KL-2, KL-1 and KL-2 strains were similar to physiological characteristics: growth was observed at pH 5 to 10 and in 0% to 6.0% NaCl. Two strains did not growth at a concentration above 7.0% NaCl and pH10. This bacterium was infected into health catfish hypodermically. Such injection was found to induce haemorrhagic ulcers very similar to those observed in naturally infected fish. At 24h post-infection, the red spot developed around the injection site and grew bigger to from a red sport area. At 120h post-infection, the muscle ncerosis was extended near the ventral fin. The seventy percent lethal dosage was appeared to water temperature at 25℃. Two strains were tested for drug senistiveity by plate method. KL-1 and KL-2 strains were sensitive to GM. K, N, S and SxT, but resistant to CF. L and VA

어병 세균에 대한 쑥 정유의 항균력 검색

김영길(Young Gill Kim),노범진(Bum Jin Rho),이근광(Keun Kwang Lee) 한국어병학회 1994 한국어병학회지 Vol.7 No.2

The antimicrobial activity of Artemisia princeps var. orientalis essential oil against a partial fish pathogenic bacteria was examined. The growth of Aeromonas hydrophila, Aeromonas salomonicida, Aeromonas sorbia, Edwardsiella tarda and Streptococcus sp. (yellowtail) were inhibited at concentrations of 1,000 to 2,000 ppm. The A. salmonicida was inhibited at 1,000 ppm, A. hydrophila, A. sorbia, E, tarda and Streptococcus sp. (yellowtail) at 1,500 ppm, but Vibrio anguillarum, Vibrio ordalii, Edwardsiella ictaluri and Streptococcus sp. (SF 1) were grown on 100-2,000 ppm.

한국산 메기의 질병에 관한 연구 : 3 . Edwardsiella ictaluri 감염증

김영길(Young Gill Kim),박성우(Sung Woo Park) 한국어병학회 1994 한국어병학회지 Vol.7 No.2

A new bacterial infection occurred among the cultured Korean catfish, Silurus asotus, in Chunbuk prefecture, Korea, 1993. This disease produced about 30% mortality in the fish for 4 months. The diseased fish was swimming listless at the water surface with head up and tail down, sometimes spinnig in circles. The most outstanding clinical sign was ulceration on the skull and at the base of the pectoral fins. The causative organism was isolated from the brain, kidney, spleen and liver of diseased fish, and identified as Edwardsiella icaluri by the biochemical and biophysical characteristics. After intraperitoneal innoculaton of the isolate, the pathogenicity was proved positive for Korean catfish, S asotus, and channel catfish, Ictalurus punctatus, but negative for carp, Cyprinus carpio.