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Performance Evaluation of the Preanalytic Module of the ACL TOP 750 Hemostasis Lab System
권우재,안정열,박필환,Yiel Hea Seo,김경희,서자영,정지훈,이환태 대한진단검사의학회 2018 Annals of Laboratory Medicine Vol.38 No.5
Dear Editor, Hemolysis, icterus, and lipemia are important preanalytic sources of errors in coagulation testing of blood samples, besides sample-to-anticoagulant ratio, transport stability, and storage temperature. Lipemia (13%), hyperbilirubinemia (11%), and hemolysis (4%) in whole blood cause errors in coagulation testing [1, 2]. Hemoglobin, bilirubin, and triglyceride are major interfering substances that affect test results by spectral overlap [3, 4]. Hemolytic, icteric, and lipemic (HIL) sample check functions have been implemented in the preanalytic module of the ACL TOP family 50 series spectrophotometric hemostasis equipment (Instrumentation Laboratory Company, Bedford, MA, USA) to support preanalytic sample evaluation. We evaluated the performance of the ACL TOP 750 preanalytic module, using patient samples.
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한 3차 대학병원의 혈액배양 분석(2006년-2015년)
서일혜,정지훈,이환태,권우재,박필환,안정열,김경희,서자영 대한임상미생물학회 2017 Annals of clinical microbiology Vol.20 No.2
Background: Cumulative blood culture data provide clinicians with important information in the selection of empiric therapy for blood stream infections. Methods: We retrospectively analyzed blood culture data from a university hospital during the period from 2006 to 2015. Only the initial isolates of a given species for each patient were included. Results: The number of blood cultures per 1,000 inpatient-days increased from 64 in 2006 to 117 in 2015. The ratio of significant pathogens to total isolates was 0.56-0.63. The most common organisms were Escherichia coli in 2006-2010 but changed to coagulase-negative staphylococci (CoNS) in 2011. The proportion of Staphylococci aureus was decreased during the study period, but Klebsiella pneumoniae was increased. Enterococci were increased, especially E. faecium, which was more frequently isolated than E. faecalis in 2015. Pseudomonas aeruginosa was decreased during the study, but Acinetobacter baumannii was increased. The prevalence of methicillin-resistant S. aureus (MRSA) changed from 62.2% to 53.9%, while vancomycin-resistant E. faecium increased to 35.8%. Extended-spectrum beta-lactamase (ESBL)-producing E. coli and K. pneumoniae increased to 25% and 34%, respectively, in 2015. Starting in 2008, three E. coli and 11 K. pneumoniae isolates were carbapenem-resistant Enterobacteriaceae (CRE), and three were carbapenemase- producing Enterobacteriaceae (CPE). The prevalence of imipenem-resistant A. baumannii rapidly increased during the study period. Conclusion: About 60% of all blood isolates were significant pathogens. The most common isolates changed from E. coli to CoNS in 2011. ESBL-producing E. coli and K. pneumoniae, vancomycin-resistant E. faecium, and imipenem-resistant A. baumannii were increased during the study, while the proportion of MRSA tended to decrease slightly. Of the total isolates, 14 were CRE, and 3 were CPE. (Ann Clin Microbiol 2017;20:-41)
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