합성아미노산 첨가수준에 따른 부로일러 사료의 단백질 절약효과

강한석,곽종형 ( Han Suk Kang,Chong Hyung Kwack ) 한국축산학회 1983 한국축산학회지 Vol.25 No.2

In order to investigate the protein sparing effect of synthetic amino acids (dl-methionine, l-lysine) on the broiler ration, this experiment was conducted with a total of 240 Piterson starting chickens for a period of 4 weeks. 8 treatments of (1) control, CP 21%,(2) basal, CP 19%, (3) basal +lysine 0.15%, (4) basal + lysine 0.3%, (5) basal + methionine 0.1% (6) basal + methionine 0.2%, (7) basal + lysine 0.15% + methionine 0.1%, (8) basal + lysine 0.3% + methionine 0.2% were employed with 3 replicates in this experiment. Metabolism trial was carried out at the end of feeding trial. The results obtained were summarized as follows; 1. Body gain was higher in the basal + lysine 0.15% + methionine 0.1% treatment than in the other treatments. There was significant difference (P$lt;0.01) between the basal+lysine 0.15% + methionine 0.1% treatment and other treatments. 2. Feed intake was higher in the control treatment .than in the other treatments. However, no significant difference was found out between the control treatment and the lysine 0.15% + methionine 0.1% treatment. However, there was significant difference (P$lt;0.05) between the control treatment and the lysine 0.3% + methionine 0.2% treatment. Also, significant difference (P$lt;0.01) was found out between the control treatment and other treatments. 3. Basal + lysine 0.15% + methionine 0.1% treatment was best in the feed efficiency but basal treatment basal + lysine 0.15% treatments were lower than other treatments. 4. In the nutrient: utilization, utilization of dry matter, crude protein and crude fat was highest in the control, lysine 0.15% + methionine 0.1% and the control treatment respectively. Then, significant difference (P$lt;0.05) was found between the control treatment and the basal treatment in the crude fat utilization. Utilization of carbohydrate was higher in the lysine 0.15% + methionine 0.1% treatment than in the other treatments. Significant difference (P$lt;0.05) was found between the lysine 0.15% + methionine 0.1% treatment and the methionine 0.2%, basal treatment, and there was significant difference (P$lt;0.01) between the lysine 0.15% + methionine 0.1% treatment and other treatments in the utilization of carbohydrate. 5. In the economical analysis, feed cost required per kg body gain was lowest in the lysine 0.15%+ methionine 0.1% treatment and next in the control treatment. Therefore, based on the results of this experiment, it may be concluded that the supplementation of lysine 0.15% + methionine 0.1% with the broiler ration of crude protein 19% would spare 2% protein feed.

Investigation of Single Nucleotide Polymorphisms in Porcine Candidate Genes for Economic Traits in the Commercial Pig Breed

Sang Wook Kim(김상욱),Mi Rang Lee(이미랑),Han Seok Kang(강한석),Seon Ku Kim(김선구),Teak Soon Shin(신택순),Hong Gu Lee(이홍구),Hae Yeal Jeon(전해열),Kwan Suk Kim(김관석),Chang Hee Do(도창희),Bong Hwan Choi(최봉환),Tae Hun Kim(김태헌) 한국생명과학회 2008 생명과학회지 Vol.18 No.6

돼지 2번 염색체의 육질 관련 양적 경제형질에 관한 연구보고가 몇몇 이루어 지고 있다. 양돈업계에서 DNA 기술을 이용한 염색체 정보를 활용하기 위해 본 연구에서는 13개의 후보 유전자에서 생성된 중합효소연쇄반응(PCR) 생성물을 비교 재서열 함으로써 단일염기변이(SNP) 표지들을 개발했다. 11개의 중합효소연쇄반응 생성물에서 296 bp마다 에서 평균 하나의 SNP, 총 34개의 SNP를 발견하였다. 또한 11개의 SNP에 대해 PCR 제한효소 길이 절편길이 다형성(RFLP) 분석을 전개한 후, 이를 대한민국 상업돈 4품종 개체군의 유전자형을 분석하는데 활용하였다. 본 연구는 유용한 단일염기변이를 식별하고 돼지 개체군 내 경제적으로 중요한 특성들과 SNP의 연관성을 확인하는 데 그 목적이 있다. Several studies reported quantitative trait loci (QTL) for meat quality on porcine chromosome 2. For application of the chromosomal information to pig industry through using DNA technology, single nucleotide polymorphism (SNP) markers are developed by comparative re-sequencing of polymerase chain reaction (PCR) products of 13 candidate genes. A total of 34 SNPs were identified in 11 PCR products, an average of one SNP in every 296 bp.PCR restriction fragment length polymorphism (RFLP) assays were developed for 11 SNPs and used to genotype four commercial pig populations in Korea. The SNP markers were used to map candidate genes in QTL and to clarify the relevance of SNP and quantitative traits.

A Study on the Prolactin Receptor 3 (PRLR3) Gene and the Retinol-binding Protein 4 (RBP4) Gene as Candidate Genes for Growth and Litter Size Traits of Berkshire in Korea

Chang-Hee Do(도창희),Seon-Ku Kim(김선구),Han-Suk Kang(강한석),Teak-Soon Shin(신택순),Hong-Gu Lee(이홍구),Seong-Keun Cho(조성근),Kyung-Tak Do(도경탁),Ji-Na Song(송지나),Tae-Hun Kim(김태헌),Bong-Hwan Choi(최봉환),Byung-Chan Sang(상병찬 한국생명과학회 2010 생명과학회지 Vol.20 No.6

본 연구는 버크셔 품종에서 PRLR3와 RBP4 후보유전자의 두 개의 대립유전자가 산육형질과 번식형질에 미치는 영향을 조사하였다. 5,919두의 혈통자료, 3,480두의 산육기록과, 244두의 모돈의 775마리의 산자기록을 이용하여 유전능력 평가를 수행하였다. 유전자형 분석은 144두와 156두에서 PRLR3와 RBP4 유전자의 유전자형을 각각 분석하였다. 평가된 개체들의 육종가를 이용 두 마커의 유전자형 효과와 유의 확률을 추정한 결과 PRLR3 유전자는 번식형질의 총산자수(TBN)와 생존산자수(NBA)에서 -0.28과 -0.13두의 상가적 효과를 각각 나타내었다. RBP4 유전자는 일당증체량에서 10.58 g의 우성적 효과를 나타내었다. 그러나 RBP4 유전자의 다형성은 번식형질의 총산자수(TBN)와 생존산자수(NBA)에서 -0.34와 -0.33두의 상가적 유전적 효과를 각각 나타났다. 따라서 PRLR3와 RBP의 B 대립유전자를 선호하는 MAS (Marker Assist Selection) Scheme은 버크셔 품종의 산자수의 개량에 이용할 수 있을 것이다. Two diallelic markers at candidate gene loci, the prolactin receptor 3 (PRLR3) gene and the retinol-binding protein 4 (RBP4) gene were evaluated for their association with growth and litter size traits in Berkshire. Genetic evaluation was conducted for 5,919 pigs with pedigree information, which included 3,480 growth performance records and 775 litter size records of 224 sows. From the same herd, genotyping was carried out on 144 and 156 animals for PRLR3 and RBP4, respectively. After assigning a genotype to subjects in which both parents had a homozygous genotype, numbers of genotyped animals increased to 474 and 338, for the PRLR3 gene and RBP4 gene, respectively. The genotype effects of two markers were estimated with breeding values of the genotyped animals. The additive effects of total number of piglets born and number of piglets born alive in the PRLR3 locus were -0.28 and -0.13, respectively. The dominance effect of the RBP4 locus on average daily gain was -10.58 g. However, the polymorphism of the RBP4 locus in total number of piglets born and number of piglets born alive has shown -0.34 and -0.33 of the additive genetic effects. In view of the results, MAS (marker-assisted selection) favoring B alleles of RBP4 and PRLR3 loci could potentially accelerate the rate of the genetic improvement in the litter size traits.

단위가축영양 : 토종 육계에 있어서 복합 생균제 및 면역증강제의 항생제 대체효과

이상범 ( Sang Bum Lee ),김병극 ( Byung Kuk Kim ),박창호 ( Chang Ho Park ),박건현 ( Gun Hyun Park ),김영성 ( Yong Cheng Jin ),강한석 ( Han Suk Kang ),김영철 ( Young Chul Kim ),김윤칠 ( Youn Chil Kim ),배승철 ( Sung Chul Bai ),김선 한국동물자원과학회(구 한국축산학회) 2011 한국축산학회지 Vol.53 No.5

This study was conducted to investigate the effects of dietary probiotics and immunomodulator on growth performance, carcass characteristics, fecal NH3 content and pathogenic bacteria counts in ileum and cecum and ileum of broiler chicken(Korean native chicken, HanHyup No.3). A total of 120(day-old) chicks were randomly divided into 5 treatments with 3 replicates and there were 8 birds per replicate. Dietary treatments consisted of five diets; the corn-soybean based control diet(C), the diet containing antibiotics (Avilamycin) 10 ppm(T1), the diet containing probiotics 1[(Lactobacillus(4.45×10(6))+yeast(1.51×10(6))+Bacillus subtilis (3.50×10(5))] at 0.5% level(T2), probiotics 2 [(Lactobacillus (6.70×10(7))+yeast(3.10×10(6))] at 0.5% level in diet(T3), and the diet containing probiotics 3[T2+β-glucan+organic acid](T4) and raised for 9 weeks. There were no significant differences among treatments in weight gain, feed intake and feed conversion. Carcass ratios of broilers were higher in T3 and T4 than other treatments, however, the differences were non-significant. Internal organs and liver, heart weight were significantly increased in T4 (p<0.05) compared to other treatments. The fecal NH3 gas content was decreased(p<0.05) in antibiotics fed group than others. However, probiotic fed groups were not different when compared with control. The number of Salmonella and E. coli in cecum were reduced in the group supplemented with probiotics and immunomodulator compared to the antibiotics(p<0.05). In this experiment, we showed that diets containing pro-biotics and immunomodulator were capable of an alternative to antibiotics.