상황버섯 추출물 음료의 섭취가 흰쥐의 근육 및 혈액의 대사적 변인에 미치는 영향

강준용 ( Jun Yong Kang ),권대근 ( Dae Keun Kwon ),송영주 ( Young Ju Song ) 한국운동영양학회 2008 Physical Activity and Nutrition (Phys Act Nutr) Vol.12 No.1

8주간의 해양심층수 섭취와 수영운동이 흰쥐의 지질대사 및 항산화 효소에 미치는 영향

강준용(Kang Jun-Yong),권대근(Kwon Dae-Keun),송영주(Song Young-Ju) 한국체육과학회 2011 한국체육과학회지 Vol.20 No.1

This study was conducted to investigate the effects of 8 weeks deep-sea water supplementation and swimming exercise on lipid metabolism and antioxidant enzymes of rats. Thirty-two SD male rats, 6 weeks of age, were randomly divided into 4 groups: (HS : high fat diet sedentary group n=8); (HE: high fat diet exercised group n=8); (DS: deep-sea water supplemented sedentary group n=8); (DE : deep-sea water supplemented exercised group n=8), given a high fat diet and free access to tap water (HS and HE) or deep-sea water (DS and DE) for 8 weeks, and housed in groups of two per cage under controlled temperature(23±1℃) and relative humidity(50±5%). The light/dark cycle was automatically controlled(alternation 12-h periods) and lighting was begun at 8:00. The animals in the swim groups swam for 60 min/day-1, 5 days/wk, 2% load/weight for 8weeks in a large plastic tube filled with water(temp. : 35±1℃, depth : 50㎝, radius : 25㎝). In the results, Serum TG level of DS group was lower than HS group, but there was no significantly difference. Furthermore, PPAR-γ and PGC-1α were slightly increased in deep-sea water supplemented groups. The Mn-SOD protein expression of DS group was significantly higher than HS group (p<.01) and the value of DE was slightly higher than HE group. These results suggest that deep-sea water supplementation and exercise has a positive effect on lipid metabolism and antioxidant enzymes in rats by decreasing serum TG levels, and increasing PPAR-γ, PGC-1α, and Mn-SOD protein expression.

언어 모델을 이용한 시각 묘사 생성과 CLIP 기반 제로샷 이미지 분류의 상관관계 연구

강준용(Junyong Kang),전찬(Chan Jeon),강유석(Yuseok Kang),정영민(Yeongmin Jeong) Korean Institute of Information Scientists and Eng 2023 한국정보과학회 학술발표논문집 Vol.2023 No.12

복합 트레이닝이 고교 레슬링 선수의 기초체력과 피로회복 및 운동 유발성 스트레스 반응에 미치는 영향

박은철 ( Eun Chol Park ),강준용 ( Jun Yong Kang ),권대근 ( Dae Keun Kwon ),송영주 ( Young Ju Song ) 대한무도학회 2012 대한무도학회지 Vol.14 No.3

연구는 복합트레이닝이 고교 레슬링 선수의 기초체력과 일회성 무산소 운동에 따른 피로회복 및 운동 유발성 스트레스 반응에 미치는 영향에 대해 연구하였다. 본 연구에서는 8명의 고교 레슬링선수를 대상으로 3개월간의 복합 트레이닝을 실시하였으며, 대조군은 일반 남자 고교생 8명으로 3개월간 특별한 운동을 실시하지 않았다. 모든 피험자들은 3개월간의 복합 트레이닝 전·후에 기초체력검사(좌전굴, 눈감고 외발서기, 악력, 배근력, 서전트점프, 윗몸일으키기, 전신반응), wingate test(peak power, mean power, fatigue index) 및 혈청분석(C-reactive protein: CRP, lactate dehydrogenase: LDH, lactate)을 실시하였다. 본 연구 결과, 기초체력에서는 유연성, 눈감고 외발서기, 윗몸일으키기 및 전신반응 능력이 복합 트레이닝 후 유의한 개선 효과를 나타내었다. 그리고 혈청 CRP 농도는 측정시기에 의한 유의한 차이가 나타났으며, 측정 시기와 그룹 간 상호작용효과가 나타났다. 또한, LDH 활성과 lactate 농도는 측정시기에 의한 차이가 있는 것으로 나타났다. 본 연구의 결과로부터 고교레슬링 선수들을 대상으로 한 복합 트레이닝은 기초체력의 향상과 함께 일회성무산소 운동에 따른 피로와 운동 유발성 스트레스를 억제하는 긍정적인 효과가 있는 것으로 시사되었다. The purpose of this study was to investigate the effects of combined training on basic physical strength, and fatigue recovery and exercise-induced stress response in high school wrestling athletes. Eight male high school students were performed combined training for 3 months. Basic physical strength test and wingate test were tested before and after participation in combined training program for 3 months. In the basic physical strength test, flexibility, closed eyes with single leg stand, sit-ups, and reaction time were significantly improved in the post training, respectively. Furthermore, the CRP was significantly different between pre- and post-, and the difference appeared in interaction of time and group. Additionally, LDH and lactate were showed significantly different between pre- and post-. In conclusion, combined training have a crucial role in amelioration of fatigue recovery and acute exercise induced stress response as well as improve basic physical strength in high school wrestling athletes.

Effects of Opuntia humifusa Supplementation on Lipid Peroxidation and SOD Protein Expression in the Liver, Kidney, and Skeletal Muscle of Rats Fed a High-fat Diet

Daekeun Kwon(권대근),Junyong Kang(강준용),Jinho Park(박진호),Sungpil Ryu(류승필),Youngju Song(송영주) 한국생명과학회 2012 생명과학회지 Vol.22 No.7

본 연구는 안정 시 고지방식이 흰쥐의 신장, 간장 그리고 골격근에서 지질과산화와 SOD 단백질 발현에 손바닥 선인장 보충이 미치는 효과에 대하여 연구하였다. SD계 수컷 흰쥐 16마리를 무작위로 대조군(CG, n=8)과 실험군(EG, n=8)으로 분류하였다. 8주 동안 대조군은 고지방식이를 부하하였으며, 실험군은 5% 손바닥선인장을 보충식이하였다. 본 실험결과, 간장과 신장의 MDA 농도는 EG군이 CG군에 비해 유의하게 낮게 나타났다(p<0.01). 또한 골격근의 MDA 농도도 EG군이 CG군에 비해 높은 경향이 나타났으나, 통계적으로 유의한 차이는 나타나지 않았다. Cu,Zn-SOD 단백질 발현은 신장에서EG군이 CG군에 비해 유의하게 증가하였다(p<0.01). Mn-SOD 단백질 발현은 골격근에서 EG군이 CG군에 비해 유의하게 증가하였다(p<0.01). 이상의 결과로부터 손바닥선인장 보충이 고지방식이 흰쥐의 지질과산화 억제작용과 SOD단백질 발현이 기관특이적인 반응의 항산화 효과가 있는 것으로 나타났다. This study was conducted to investigate the effects of Opuntia humifusa supplementation on lipid peroxidation and superoxide dismutase (SOD) protein expression at resting state in various organs of rats fed a high-fat diet. Sixteen Sprague-Dawley male rats, 6 weeks of age, were randomly divided into two groups: a control diet group (CG, n=8) and an experimental diet group (EG, n=8). They were given a high-fat diet (CG) or a diet supplemented with 5% of O. humifusa (EG) or 8 weeks. The results showed that the malondialdehyde (MDA) levels of the kidney and the liver were significantly lower in the EG group than in the CG group (p<0.01). In addition, the MDA levels in the skeletal muscle of the EG group tended to be lower than those in the CG group, but this difference was not significant. The Cu, Zn-SOD protein expression in the kidney of the EG group was significantly increased compared with that of the CG group (p<0.01). The Mn-SOD protein expression in the skeletal muscle of the EG group was significantly increased compared with that of the CG group (p<0.01). These results suggest that O. humifusa supplementation has antioxidative properties, which are exerted in a specific organ manner, and that it inhibits the action of lipid peroxidation and the expression of SOD in rats fed a high-fat diet.

The effect of powdered Opuntia hmifusa supplementation on suppression of swimming exercise-induced bone loss in growing male rats

Seol, Byung-Hwan(설병환),Kang, Jun-Yong(강준용),Kwon, Daek-Gun(권대근),Park, Jin-Ho(박진호),Song, Young-Ju(송영주) 한국체육과학회 2013 한국체육과학회지 Vol.22 No.6

본 연구는 천년초 처치가 성장기 흰쥐의 수영운동에 따른 골소실 억제에 어떠한 효과가 있는지에 대해 연구하였다. 32마리의 수컷 SD 쥐를 고지방식이 대조군(n=8), 고지방식이 운동군(n=8), 5% 천년초 처치 대조군(n=8), 5% 천년초 처치 운동군(n=8)으로 무선 배정하였다. 본 연구에서 사용한 고지방 식이의 조성은 탄수화물 48%, 지방 20% 및 단백질 20%로서 AIN-76G를 기준으로 하여 각각 치환하여 제조하였으며, 천년초 식이는 고지방식이의 조성에서 각각 탄수화물, 지방, 단백질 및 식이섬유를 치환하여 제조하였다. 연구결과, 혈청 오스테오칼신 농도는 천년초 처치군이 고지방식이군에 비해 유의하게 높은 것으로 나타났으며, 혈청 부갑상선호르몬 농도는 OE군이 HE군에 비해 유의하게 낮은 것으로 나타났다. 그리고 대퇴골의 BMC와 BMD는 HE군이 HS군에 비해 유의하게 낮은 것으로 나타났으며, 대퇴골과 경골의 BMC 및 BMD는 OE군이 HE군에 비해 유의하게 높은 것으로 나타났다. 또한, 상완골의 BMD와 경골의 파단강도는 OE군이 HE군에 비해 각각 유의하게 높은 것으로 나타났다. 따라서 본 연구의 결과를 종합해보면 성장기 흰쥐에게 천년초의 처치는 부갑상선호르몬 분비의 억제와 오스테오칼신 농도를 증가시킴으로써 BMD와 BMC를 증가시켜 수영운동에 의한 골소실 예방에 긍정적인 효과가 있음을 시사하였다.

천년초(Opuntia Humifusa) 섭취와 수영운동이 고지방식이 흰쥐의 지질 과산화 및 항산화 단백질 발현에 미치는 영향

박진호(Park, Jin-Ho),강준용(Kang, Jun-Yong),권대근(Kwon, Dae-Keun),송영주(Song, Young-Ju) 한국체육과학회 2013 한국체육과학회지 Vol.22 No.3

This study was conducted to investigate the effects of Opuntia Humifusa supplementation and swimming exercise of 8 weeks on lipid peroxidation and antioxidant protein expression of rats fed a high-fat diet. Thirty-two Sprague-Dawely male rats, 6 week of age, were randomly divided into 4 groups: high fat diet sedentary group(HS, N=8), high fat diet exercised group(HE, n=8), Opuntia Humiifusa supplemented sedentary group(OS, n=8), Opunta Humifusa supplemented exercise gurop(OE, n=8). They were given a high fat diet (HS and HE) or supplemented 5% of Opuntia Humifusa(OS and OE) for 8 weeks. The animals in the swim groups swam for 60 min/day, 5day/wk for 8 weeks in a plastic tube filled with water(temp: 35±1℃, depth 50cm, radius: 25cm). In the results, the body weight were significantly lower in the HE and OS group than that of the HS group(p<.01, p<.05). The muscle MDA(malondialdehyde) levels of OS and OE groups was significantly lower than those of the HS and HE groups, respectively(p<.01, p<.05). In addition, GPx(glutathione peroxidase) protein expressions in the soleus muscle of HE and OS groups was significantly increased compared to those of HS group(p<.01, p<.05). The Cu,Zn-SOD and Mn-SOD protein expressions was tended to higher value than those of th HS and HE groups. From these results, it was suggested that Opuntia Humifusa supplementation palys a positive role for the inhibition of lipid peroxidation due to increasing muscle GPx protein expression and decreasing muscle MDA content.

산양삼을 첨가한 고지방식이 흰쥐 골격근의 지질과산화 및 항산화 단백질 발현 효과

권대근 ( Dae Keun Kwon ),강준용 ( Jun Yong Kang ),송영주 ( Young Ju Song ),김판기 ( Pan Gi Kim ),서효빈 ( Hyo Bin Seo ),류승필 ( Sung Pil Ryu ) 한국산림과학회 2012 한국산림과학회지 Vol.101 No.1

We investigated the effect of mountain ginseng-added high fat diet supplementation on lipid peroxidation and antioxidant protein expressions in rats. Thirty-two male rats were randomly divided into 4 groups; HS (high-fat diet sedentary group, n=8), MG1 (0.5% mountain ginseng-added diet group, n=8), MG2 (1% mountain ginseng-added diet group, n=8) and MG3 (2% mountain ginseng-added diet group, n=8). They have fed the diet for 4 weeks, The blood triglyceride were significantly lower in the MG1 and MG2 groups than that of the HS group, The blood HDL-cholesterol were significantly higher in the MG3 group than that of the HS and MG2 groups. The muscle glycogen contents of the MG2 and MG3 groups were significantly higher than that of HS and MG1 groups. The MDA contents in the MG1, MG2 and MG3 groups tended to lower than the HS group. The GPx protein expression in the gastrocnemius muscle of the MG2 group was significantly increased compared to that of the HS group. The Cu, Zn-SOD protein expression in the gastrocnemius muscle of the MG1 and MG2 groups was significantly increased compared to that of the MG3 group. The Mn-SOD protein expression in the MG1, MG2 and MG3 groups tended to higher than the HS group. From these results, it was suggested that mountain ginseng-added diet may have an crucial role on decreased MDA levels and increased antioxidant function in the skeletal muscle of rat fed a high fat diet.

Opuntia humifusa Supplementation Reduces Fat Weight by Increasing PPAR-γ and PGC-1α Protein Expression in the Skeletal Muscle of Rats

Daekeun Kwon(권대근),Junyong Kang(강준용),Jaeseung Kim(김재승),Youngju Song(송영주) 한국생명과학회 2014 생명과학회지 Vol.24 No.1

본 연구는 안정 시 고지방식이 흰쥐의 골격근에서 PPAR-δ, PPAR-γ 그리고 PGC-1α 단백질 발현에 손바닥선인장 보충이 미치는 효과에 대하여 연구하였다. SD계 수컷 흰쥐 16마리를 무작위로 대조군(CG, n=8)과 실험군(EG, n=8)으로 분류하였다. 8주 동안 대조군은 고지방식이를 부하하였으며, 실험군은 5% 손바닥선인장을 보충식이하였다. 본 실험결과, 복부지방과 고환부 지방 중량은 EG군이 CG군에 비해 유의하게 낮게 나타났다(p<0.01). 또한 혈당, 중성지방, 총콜레스테롤의 농도도 EG군이 CG군에 비해 유의하게 낮게 나타났다(p<0.01). 한편, 골격근에서 PPAR-γ와 PGC-1α 단백질 발현은 EG군이 CG군에 비해 유의하게 높게 나타났다(p<0.05). 이상의 결과로부터 손바닥선인장 보충이 고지방식이 흰쥐의 혈당과 중성지방 농도의 감소와 골격근에서 PPAR-γ와 PGC-1α 단백질 발현을 증가시킴으로서 체지방을 감소시켜 체중증가 억제에 긍정적인 영향을 미치는 것으로 나타났다. This study was conducted to investigate the effects of supplementation with Opuntia humifusa on the expression of peroxisome proliferator-activated receptor-delta (PPAR-δ), peroxisome proliferator-activated receptor-gamma (PPAR-γ) and peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1α) in the skeletal muscle of rats fed a high-fat diet. Sixteen Sprague-Dawley male rats at 6 weeks of age were randomly divided into 2 groups: a control diet group (CG, n=8) and an experimental diet group (EG, n=8). The rats were fed a high-fat diet (CG) or a high-fat diet supplemented with 5% O. humifusa (EG) for 8 weeks. The results showed that the abdominal fat pad and epididymal fat pad weights were significantly lower in the EG than in the CG (p<0.01). In the blood, serum glucose, triglycerides, and total cholesterol in the EG group were lower than in the CG (p<0.01). The expression of PPAR-γ and PGC-1α protein in the skeletal muscle of the EG was increased compared with that of the CG (p<0.05). These results indicate that 8 weeks of O. humifusa supplementation lowers serum glucose and triglyceride levels and suppresses weight gain by reducing fat weight through an increase in the expression of PPAR-γ and PGC-1α in the muscle tissue of rats.

동결건조한 천년초(Optuntia Humifusa) 분말섭취가 20대 남성의 운동 유발성 스트레스반응에 미치는 영향

박진호(Park, Jin-Ho),강준용(Kang, Jun-Yong),권대근(Kwon, Dae-Keun),박재용(Park, Jae-Yong),송영주(Song, Young-Ju) 한국체육과학회 2014 한국체육과학회지 Vol.23 No.3

The purpose of this study was evaluate the effect of Opuntia Humifusa(OH) supplementation on exercise-induced stress reaction in twenty male for 2 weeks. All the subjects were performed a single bout of exercise by using a cycle ergometer before and after 2 weeks of treatment. Experimental group consumed OH (6mg/day) for 2weeks. Collected blood samples were used for analyzing glucose, triglyceride(TG), total cholesterol(TC), and high-density lipoprotein cholesterol(HDLC). In addition, exercise-induced stress markers including creatine phosphokinase(CPK), C-reactive protein(CRP), lactate dehydrogenase(LDH), uric acid(UA), IL-1beta, and IL-6. In the results, glucose were significantly decreased in experimental group after 2 weeks of OH supplementation. However, although the CRP level was tend to decreased after 2 hours recovery of experimental group in the post period, exercise-induced stress markers were not showed significantly change after OH supplementation. These results suggested that 2 weeks of OH supplementation showed crucial role in lowering blood glucose level and a possibility of protective role in cardiovascular stress after a single bout of strenuous exercise in twenty male.