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崔昇勳,安圭錫,成熙根 慶熙大學校韓醫科大學韓醫學硏究所 1999 慶熙韓醫大論文集 Vol.22 No.1
This experimental study was carried out to evaluate the effects of Curcuma on angiogenic inhibition mechanism. In order to investigate the effects of Curcuma on angiogenic inhibition mechanism evaluate cell survival rate by MTT assay, cell adhesive inhibition effect, DNA fragmantaion analysis. Nuclear Condensation assay FACScan analysis, angiogenesis inhibition assay. Immunocytochemistry analysis, RT-PCR for mRNA expression. Western Blot analysis. The results were summarized as follows : 1. The IC50 was 10㎍/ml to ECV 304 cell. 2. The cell adhesive inhibition ability was strong from 5㎍/ml. 3. The G0/G₁arrest peak was existed on ECV304 cell-line. 4. The cells on Collagen plate were inhibition of proliferation and inducement of apoptosis by Cur water extract. 5. Lumen formation was inhibited by Cur water extract. 6. LFA-1 and ELAM-1's expression were inhibited by Cur water extract, they are commenly participation on inflammation and tumor regeneration. 7. The expression of MMP-9 and uPA were inhibited by Cur water extract. 8. The expression of integrin αvβ₃ was inhibited by Cur water extract. 9. The expression of intracellular molecule were successively inhibited by Cur water extract therefore the proliferation of ECV304 cell line was stopped and apoptosis was induced. According to the results Curcuma has been shown to be a key antagonist of intergrin αvβ₃and which seems to be apoptosis by P53 through Flow cytometry. This report demonstrated that expression of MMP-9 and uPA blocked to under angiogenesis model. Thus, we suggested that Curcuma water block angiogenesis by inducing unscheduled apoptosis in ECV304 and ECVPAR cell lines and another orient herbal medicine that treat Qi-stagnation and Blood-stasis type also have angiogenic inhibition effects.
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鬱金이 Angiogenesis의 抑制機轉에 미치는 影響
崔昇勳,安圭錫,成熙根 대한동의병리학회 1999 동의생리병리학회지 Vol.13 No.2
鬱金이 ECV304 cell line 과 ECVPAR cell line에서의 angiogenesis 억제기전에 미치는 영향을 알아보기위하여 실험한 결과 다음과 같은 결과를 얻었다. 첫째, 세포 독성은 ECVPAR cell line에서 10㎍/ml였다. 둘째, 세포부착능력은 ECV304 cell line 과 ECVPAR cell line에서 5㎍/ml부터 강한 저해효과를 보였다. 셋째, ECV304 cell에서 Go/G1 arrest peak가 존재 하였다. 넷째, ECV304 cell line과 ECVPAR cell line에서 Collagen plate에서의 증식억제 및 apoptosis를 유도하였다. 다섯째, ECV304 cell line에서 angiogenesis의 lumen형성을 저해함을 알 수 있었다. 여섯째, ECV304 cell line 과 ECVPAR cell line에서 inflammation 및 tumor regeneration에 공통적으로 관여하는 LFA-1 및 ELAM-1의 발현과 기능을 저해함을 알 수 있었다. 일곱째, ECV304 cell line 과 ECVPAR cell line에서 MMP-9과 uPA의 발현이 억제됨을 알 수 있었다. 여덟째, ECV304 cell line 과 ECVPAR cell line에서 integrin ανβ3의 발현이 억제됨을 알 수 있었다. 아홉째, intracellular molecule의 발현에도 연속적으로 저해하므로써 ECV304 cell line 과 ECVPAR cell line의 증식이 중단되고 apoptosis를 유도 하게 됨을 알 수 있었다. 이상의 실험결과로 보아 鬱金이 ECV304 cell line과 ECVPAR cell line에서 발현되는 intergrin ανβ3의 발현을 억제하여 세포의 apoptosis를 유도하므로써 angiogenesis를 억제하는 효과가 있음을 알 수 있었다. This experimental study was carried out to evaluate the effects of Curcuma on angiogenic inhibition mechanism. In order to investigate the effects of Curcuma on angiogenic inhibition mechanism evaluate cell survival rate by MTT assay, cell adhesive inhibition assay, lmmunocytochemistry analysis, RT-PCR for mRNA expression, Western Blot analysis. The results were summarized as follows: 1. The IC50 was 10㎍/ml to ECV 304 cell 2. The cell adhesive inhibition ability was strong from 5㎍/ml. 3. The G0/G1 arrest peak was existed on ECV304 cell-line. 4. The cells on Collagen plate were inhibition of proliferation and inducement of apoptosis by Cur water extract. 5. Lumen formation was inhibited by Cur water extract. 6. LFA-1 and ELAM-1's expression were inhibited by Cur water extract. they are commonly participation on inflammation and tumor regeneration. 7. The expression of MMP-9 and uPA were inhibited by Cur water extract. 8. The expression of integrin ανβ3 was inhibited by Cur water extract. 9. The expression of intracellular molecule were successively inhibited by Cur water extract therefore the proliferation of ECV304 cell line was stopped and apoptosis was induced. According to the results Curcuma has been shown to be a key antagonist of intergrin ανβ3, and which seems to be apoptosis by P53 through Flow cytometry. This report demonstrated that expression of MMP-9 and uPA blocked to under angiogenesis model. Thus, we suggested that Curcuma water block angiogenesis by inducing unscheduled apoptosis in ECV304 and ECVPAR cell lines and another oriental herbal medicine that treat 뺘-stagnation and Blood-stasis type also have angiogenic inhibition effects.
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