家蠶의 Vitellogenin에 관한 硏究

尹馨珠,李相夢,文在裕 한국잠사학회 1987 한국잠사곤충학회지 Vol.29 No.1

In the hemolymph proteins of the silkworm reared with mulberry leaves and artifcial diet, electrophoretic patterns of Storege Protein 1 (SP1) and Vitellogenin(VG), changes of relative concentration of SP1, JHA effects during the developmental stages, and estimation of the molecular weight of vitellin subunits were investigated in the present study. 1. Storage Protein 1 (SP1) showed female specificity from the middle stage of the fifth instar to the end of spinning stage in the silkworm reared with mulberry leaves and artificial diet. 2. Vitellogenin (VG) showed female specificity just after pupation in the silkworm reared with mulberry leaves and artificial diet. 3. Storage Protein 2 (SP2) without female specificity was detected from the early stage of the fifth instar to the first or second day after pupation. 4. Vitellin was composed of two non-identical subunits (vitellin·heavy chain; VTL-H, vitellinlight chain; VTL-L) with molecular weight of 186,000 and 41,000. Also, there were no differences between the molecular weights of vitellin subunits obtained from silkworms reared with mulberry leaves and artificial diet. 5. Relative concentration of Storage Protein 1 (SP1) after topical application - 0, 5, and 10μg per body weight - of JHA on the 60th hour of the fifth instar showed the highest increase with the treatment of 5μg and a higher increase with the treatment of 10μg compared with the control(no topical application of JHA).

위성의 기동 성능 향상을 위한 반작용휠의 최대 용량 해석 및 최적 제어 분배 기법

윤형주,서현호,최홍택 한국항공우주학회 2014 한국항공우주학회 학술발표회 논문집 Vol.2014 No.11

본 연구에서는 피라미드 형태로 배치가 된 4 개의 반작용휠을 이용하여 위성의 기동 성능을 최적화하는 방법에 대해서 다루고 있다. 먼저 4 개의 반작용휠을 이용하여 만들어 낼 수 있는 모멘텀과 토크의 최대 용량을 3 차원 공간에서 구하는 방법을 다루고, 이를 이용하여 임의의 방향으로 자세 기동을 하는 위성의 기동 성능을 최대로 하는 반작용휠의 배치각을 구하는 방법을 제시하였다. 또한, 이렇게 계산한 반작용휠의 모멘텀/토크 가용 용량을 최대로 사용하기 위한 제어 입력 배분 기법을 제안하였는데, 이 기법은 새로 개발한 최소 무한대 놈 해법에 기초를 두고 있다. 마지막으로는, 본 연구에서 제시한 기법들을 이용한 위성 기동 성능의 향상 효과를 수치 해석 결과를 통해 입증하였다. In this study, simple methods are presented for optimal agility performance of a spacecraft with four reaction wheels in the pyramid configuration. First, it presents a simple method to visualize the momentum and the torque envelopes of the reaction wheels array in the three-dimensional space. Then, based on the geometry of the envelopes, optimization of installation angles of the pyramid configuration for maximum spacecraft agility performance is discussed. Then, the new methods are proposed to optimally distribute the three-dimensional torque or momentum commands to the four individual reaction wheels. These methods are based on the new minimum infinity-norm solution algorithm of underdetermined linear equations systems. Finally, numerical examples are presented to demonstrate the effectiveness of the developed methods.

국내 및 국외 뒤영벌 생산업체별 서양뒤영벌(Bombus terrestris)의 봉세발달 비교

윤형주,김지영,이경용,이상범,박인균,노시갑,Yoon, Hyung-Joo,Kim, Ji-Yeong,Lee, Kyeong-Yong,Lee, Sang-Beom,Park, In-Gyun,Noh, Si-Kab 한국응용곤충학회 2008 한국응용곤충학회지 Vol.47 No.1

We compared colony development and survival rate after artificial hibernation between the bumblebees, Bombus terrestris, produced from domestic and foreign companies. The number of worker and queen produced from foundation colony of three domestic and one foreign companies was 232.4-270.8 and 62.0-181.8, respectively, but there was no statistical difference. In colony development of B. terrestris queens which were terminated diapause by $CO_2$ narcosis at different companies, oviposition rate, colony foundation and progeny-queen production of bumblebees produced from domestic companies were superior to those from foreign company. But foreign company in the number of worker and queen was superior to domestic companies. On the other hand, survival rate and colony development of B. terrestris queens, which were terminated diapause by artificial hibernation at three domestic and two foreign companies, were no significant differences. These results indicate that oviposition, colony foundation, progeny-queen production, and survival rate after artificial hibernation showed no differences between the bumblebees produced from domestic and foreign companies. 국내 및 국외 뒤영벌 생산업체의 서양뒤영벌(Bombus terrestris) 산란율, 봉군형성률, 신여왕출현 봉군율 등 봉세발달 및 인공월동 후 생존율을 비교 조사하였다. 국내 3개와 국외 1개 생산업체의 창설여왕벌 봉군에서 생산된 일벌수는 232.4-270.8마리, 여왕벌수는 62.0-181.8마리로 다소의 차이는 있었으나 국내 및 국외 뒤영벌 생산업체간에 유의성은 없었다. 업체별로 생산된 여왕벌을 탄산가스처리후 사육한 결과, 산란율, 봉군형성률, 신여왕출현봉군율 등 봉세발달은 국내업체에서 생산한 서양뒤영벌이 유럽산보다 우수한 것으로 나타났으나, 일벌 및 여왕벌수는 유럽산 뒤영벌이 다소 많은 것으로 나타났다. 또한 국내 3개 뒤영벌 생산업체와 유럽 2개 업체에서 생산된 서양뒤영벌을 인공월동하여 사육한 경우에도 다소의 차이가 있었지만, 생존율 및 봉세발달에서 뚜렷한 차이가 없었다. 이와 같이 업체별로 생산된 창설여왕벌과 2세대 여왕벌의 휴면처리를 달리하여 사육한 결과, 국내 및 국외 뒤영벌 생산업체의 서양뒤영벌의 봉세 간에 차이가 없는 것으로 나타났다.

호박벌(Bombus ignitus)과 서양뒤영벌(B. terrestris)의 봉세발달 비교

윤형주,김삼은,이상범,심하식 한국응용곤충학회 2004 한국응용곤충학회지 Vol.43 No.2

토종 호박벌(Bombusignitus)과 수입 서양뒤영벌(B. terrestris)의 냉장온도별 생존율과 실내사육에 의한 봉세발달을 비교 조사하였다. -2.5, 0, 2.5, 5$^{\circ}C$등 4종류 냉장온도 중 호박벌과 서양뒤영벌 모두 2.5$^{\circ}C$에서 생존율이 가장 높았으며, 냉장 3개월까지는 호박벌이, 냉장 4개월째는 서양뒤영벌의 생존율이 다소 높게 나타났다. 한산가스처리로 휴면을 타파한 여왕별의 봉세발달을 조사한 결과, 산란율은 호박벌이 72.5%로 서양뒤명벌보다 6.1% 높았고, 산란전기간도 17.4일로 7.4일 빨랐다. 일벌이 50마리 될 때까지의 봉군형성소요일수, 수벌 및 여왕벌출현소요일수 또한 호박벌이 각각 62.8일, 66.4일, 63.0일로 서양뒤명벌보다 2.1-29.5일 빨리 출현하였다. 그러나 일벌출현소요일수는 28.6일과 28.4일로 차이가 없었다. 반면에 우수봉군의 지표가 되는 봉군형성률 및 신여왕벌출현봉군율은 서양뒤영벌이 각각 14.2%, 13.6%로서 호박벌보다 3.8-5.7배 높게 나타났다. 성충출현수 역시, 서양뒤영벌이 일벌 104.2 마리, 수벌 317.9 마리, 여왕벌 27.1 마리로 호박벌보다 1.1-1.8배 많아 봉세 발달은 서양뒤영벌이 우수한 것으로 나타났다. Chilling temperature and colony development in Korean native bumblebee, Bombus ignitus and introduced B. terrestris were firstly investigated. Among four chilling temperatures of -2.5, 0, 2.5 and 5$^{\circ}C$, 2.5$^{\circ}C$ showed the best result at the survival rate after artificial hibernation in both species. The survival rate after chilling was somewhat higher in B. ignitus until three months of cold treatment, but it was higher in B. terrestris at four months of cold treatment. In colony development of queens broken diapause by CO$_2$ treatment, oviposition rate and preoviposition period of B. ignitus were 72.5% and 17.4 days, respectively. These values were 6.1% higher and 7.4 days shorter than those of B. terrestris. The period up to colony foundation, the first male and queen emergence of B. ignitus were 62.8,66.4 and 63.0 days, respectively, and these values were 2.1-29.5 days shorter than those of B. terrestris. But, the duration up to first worker emergence of B. ignitus and B. terrexrris did not differ as 28.6 and 28.4 days, respectively. On the other hand, the rate of colony foundation and progeny-queen production of B. terrestris, which are the important indication in evaluating the quality of colony, were 14.2 and 13.5%, respectively and these values are 3.8 to 5.7 fold higher than those of B. ignitus. Besides, the numbers of progenies of B. terrestris reached 104.2 workers, 317.9 males and 21.1 queens, which corresponded to 1.1-1.8 fold those at B. ignitus. Therefore, above results showed that colony development of B. terrestris is superior to that of B. ignitus.

탄산가스 처리가 호박벌(Bombus ignitus)의 산란성 및 봉세발달에 미치는 효과

윤형주,김삼은,이상범,박인균 한국응용곤충학회 2003 한국응용곤충학회지 Vol.42 No.2

호박벌(Bombus ignitus)에 대한 탄산가스의 휴면타파 효과를 검토하였다. 65%, 99% 농도의 탄산가스처리 결과, 산란율은 무처리구의 50%에 비하여 65%와 99%의 탄산가스에 접촉시킨 시험구에서 각각 75%와 77%로 증가하였고, 첫산란소요일수 또한 무처리구에서 30일이 걸린데 비하여 65%와 99%의 시험구에서는 각각 18일과 17일로 단축되어 뚜렷한 탄산가스 처리효과가 나타났다. 그러나 65%와 99%의 시험구 간에는 차이가 보이지 않아 65-99%범위에서는 어느 농도의 탄산가스를 사용해도 좋을 것으로 생각되었다. 또한 탄산가스 최적 처리시기를 구명하기 위하여, 교미 후 1일부터 4일째까지 탄산가스를 처리한 결과, 산란성과 봉세발달이 교미 후 2일째가 가장 우수하여 최적 시기로 판명되었다. 그러나 호박벌에 대한 탄산가스 처리는 산란성과 봉세발달에는 긍정적인 효과를 보였지만, 차세대 출현수가 월동 여왕벌보다 적어 탄산가스 처리만으로는 상품성 있는 호박벌 봉군을 연중 생산하는 방법으로 부적당한 것으로 판단되었다. The effect of $CO_2$-treatment on interrupting diapause of Bombus ignitus was examined to provide a means for year-round rearing of the bumblebee. When mated young queens were exposed to 65% or 99% $CO_2$ for 30 min daily during two consecutive days, oviposition rate increased to 75% and 77%, respectively, comparing 50% in $CO_2$-untreated queens. At the same time, the days needed to first oviposition shortened to 17-18 days in $CO_2$-treated queens, comparing to 30 days in $CO_2$-untreated queens. $CO_2$-treatment at the second day after mating was appropriate to the oviposition and colony development. $CO_2$-treatment showed a positive effect on the oviposition and colony development, but less than them of overwintered queen in numbers of produced progeny. It can be concluded that $CO_2$-treatment to B. ignitus is insufficient to produce commercial grade bumblebee colony in spite of its capability for promoting oviposition, because the treatment failed to form a big colony.

국내논문정보 - 단일 혈액원에서 처음 시행한 헌혈 혈액에 대한 B형 간염 핵산증폭검사 경험

윤형주,김근옥,안현기,박지훈,정희숙,황유성,Yun, Hyeong-Ju,Kim, Geun-Ok,An, Hyeon-Gi,Park, Ji-Hun,Jeong, Hui-Suk,Hwang, Yu-Seong 대한산업보건협회 2013 산업보건 Vol.297 No.-

COMSOL MULTIPHYSICS 프로그램을 이용한 GBC-32 건식저장용기에 대한 온도분포 평가

윤형주,박광헌 한국방사성폐기물학회 2016 한국방사성폐기물학회 학술논문요약집 Vol.2016 No.05

A Combination Method of CO2-Narcosis and Cold Treatment for Breaking Diapause of Bombus ignitus and Bombus terrestris Bumblebee Queens

윤형주,이경용 한국잠사학회 2014 International Journal of Industrial Entomology Vol.28 No.2

Bumblebees are important pollinators of crops and wildflowers. Bumblebees generallyproduce one generation per year. One of the key stages for year-round rearing of bumblebeesis breaking diapause. To evaluate the effects of a combination method of CO2-narcosis andcold treatment to break the diapause of B. ignitus and B. terrestris queens, we determinedwhether this method affected their ability to establish a colony after the diapause break. The diapause treatment regimes that were utilized were CO2 (CO2–narcosis), CT-1M (coldtreatment at 5°C for 1 mo), CT-1M-CO2 (CO2–narcosis after cold treatment for 1 mo), CT-2MCO2(CO2–narcosis after cold treatment for 2 mo), CT-2M (cold treatment for 2 mo), CT-2.5MCO2(CO2–narcosis after cold treatment for 2.5 mo) and CT-2.5M (cold treatment at 5°C for 2.5mo). In view of the effects on the colony developmental characteristics of B. ignitus queens,the most favorable diapause treatment was CT-1M-CO2. A combination method of CO2–narcosis and cold temperature treatment yielded better results than that of single CO2–narcosisor cold temperature treatment on the colony development of diapause-broken B. ignitusqueens. In the case of B. terrestris queens, we concluded that a combination method of CO2and cold temperature treatment yielded better results than that of a single cold-temperature (upto 2 mo) treatment. In conclusion, the findings of the present study indicated that the combinedapplication of CO2 and cold temperature was a favorable method for the colony developmentof diapause-broken B. ignitus and B. terrestris queens compared with only CO2–narcosis orcold temperature treatments. A combination method of CO2 and cold treatment reduced theside effect of CO2–narcosis and shortened the duration of cold treatment by at least 1 mo.

연소도이득을 적용한 핵임계안전성평가를 위한 Depletion 계산코드의 검증

윤형주,홍서기,박광헌 한국방사성폐기물학회 2015 한국방사성폐기물학회 학술논문요약집 Vol.2015 No.05

FRAPCON 3.5 코드를 이용한 PWR 고연소도사용후핵연료의 열화거동 평가

윤형주,박광헌 한국방사성폐기물학회 2015 한국방사성폐기물학회 학술논문요약집 Vol.2015 No.10