RALY RNA Binding Protein-like Reduced Expression is Associated with Poor Prognosis in Clear Cell Renal Cell Carcinoma

Cui, Zhi-Wen,Xia, Ye,Ye, Yi-Wang,Jiang, Zhi-Mao,Wang, Ya-Dong,Wu, Jian-Ting,Sun, Liang,Zhao, Jun,Fa, Ping-Ping,Sun, Xiao-Juan,Gui, Yao-Ting,Cai, Zhi-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

The molecular mechanisms involved in the progression of clear cell renal cell carcinomas (ccRCCs) are still unclear. The aim of this study was to analyse the relationships between expression of RALYL and clinical characteristics. In 41 paired samples of ccRCCs and adjacent normal tissues, we used real-time qPCR to evaluate the expression of RALYL mRNA. RALYL protein levels were determined in 146 samples of ccRCC and 37 adjacent normal tissues by immunohistochemistry. Statistical analysis was used to explore the relationships between expression of RALYL and the clinical characteristics (gender, age, tumor size, T stage, N stage, M stage, survival times and survival outcome) in ccRCC. In addition, these patients were follow-up period 64 months (range: 4~116months) to investigate the influence on prognosis. We found significantly differences between ccRCC tissues and normal tissues (p<0.001, paired-sample t test) in mRNA levels of RALYL. Immunohistochemistry analyses in 146 ccRCC samples and 37 adjacent normal tissues showed significantly lower RALYL protein levels in ccRCC samples (${\chi}^2$-test, p<0.001), inversely correlating with tumour size (p=0.024), T stage (0.005), N stage (p<0.001) as well as M stage (p=0.019), but not age (p=0.357) and gender (p=0.348). Kaplan-Meier survival analysis demonstrated that people with lower level of RALYL expression had a poorer survival rate than those with a higher level of RALYL expression, significantly different by the log-rank test (p=0.011). Cox regression analysis indicated that RALYL expression (p=0.039), N stage (p=0.008) and distant metastasis (p<0.001) were independent prognosis factors for the overall survival of ccRCC patients. We demonstrated that the expression of RALYL was significantly low in ccRCC and correlated with a poor prognosis in a large number of clinical samples. Our findings showed that RALYL may be a potential therapeutic target as well as a poor prognostic factor.

Effect of Rhamnolipids on Degradation of Anthracene by Two Newly Isolated Strains, Sphingomonas sp. 12A and Pseudomonas sp. 12B

( Chang Zheng Cui ),( Chi Zeng ),( Xia Wan ),( Dong Chen ),( Jia Yao Zhang ),( Ping Shen ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.1

Anthracene is a PAH that is not readily degraded, plus its degradation mechanism is still not clear. Thus, two strains of anthracene-degrading bacteria were isolated from longterm petroleum-polluted soil and identified as Sphingomonas sp. 12A and Pseudomonas sp. 12B by a 16S rRNA sequence analysis. To further enhance the anthracenedegrading ability of the two strains, the biosurfactants produced by Pseudomonas aeruginosa W₃ were used, which were characterized as rhamnolipids. It was found that these rhamnolipids dramatically increased the solubility of anthracene, and a reverse-phase HPLC assay showed that the anthracene degradation percentage after 18 days with Pseudomonas sp. 12B was significantly enhanced from 34% to 52%. Interestingly, their effect on the degradation by Sphingomonas sp. 12A was much less, from 35% to 39%. Further study revealed that Sphingomonas sp. 12A also degraded the rhamnolipids, which may have hampered the effect of the rhamnolipids on the anthracene degradation.

"복선(福船)"과 복건해상(福建海商)에 관한 연구(硏究)

최운봉,우요동,Cui, Yun-Feng,Yu, Yao-Dong 해양환경안전학회 2007 해양환경안전학회지 Vol.13 No.4

중국 동남연해에 거주하고 있는 복건타성 거주민들은 일적부터 배를 건조하여 바다에 나가 고기를 잡았다. 따라서 그들은 풍부한 항해경험을 쌓았으며, 이는 대규모적인 해외무역을 진행할 수 있는 기초를 닦았다. 중국 당나라시기 동남연해에 거주하던 복건타성 거주민들은 생산력의 발전과 더불어 복건타성을 떠나 해외로 진출하여 해외무역에 종사하였다. 송원시기 복건타성의 해외교통이 비약적인 발전을 가져왔으며, 신상(紳商), 선상(船商), 산상(散商), 선원 및 화교상인(華僑商人)들로 구성된 복건타성의 해상들은 빠르고 안전하며, 순조롭게 목적지에 도착할 수 있는 복선(福船)을 이용하여 해외무역에 종사하였다. 복선(福船)은 복건해상(福達海商)들로 하여금 시간을 단축하고 상업기회를 신속하게 잡을 수 있어 무역의 성공률을 높이고 자금이용률을 높였다. 따라서 복선(福船)해상들은 신속한 발전을 가져왔으며, 발자취는 동아시아, 남아시아, 서아시아 및 아프리카 동부지역까지 이르렀다. 본 연구에서는 복건해상(福建海商)의 발생원인과 무역현황 및 해외무역에서 결정적인 역할을 한 복선(福船)에 대하여 논술하려고 한다. The people who lived in Fujian district along the south-eastern coast of China long ago began to construct ships and go fishing. For this reason, they grasped sound navigation skills and gained rich navigation experience. These skills and experience laid basis for the following large-scale overseas trade. In the Tang dynasty, with the development of economy, the people who lived in Fujian district started to leave home town and engage in overseas trade. In the Song dynasty and the Yuan dynasty, with the fast development of overseas communication, more and more Fujian maritime merchants, navigating the Fujian ships which were safe, fast and could arrive at destination on time, engaged in overseas trade. The Fujian ships made it possible for the Fujian maritime merchants to shorten trade time, catch business opportunity, level up the chance of success in trade and make full use of capital. As a result of this, Fujian maritime merchants gained fast development and they reached far areas such as eastern Asia, south-eastern Asia, southern Asia, and eastern Africa. This study deals with the issues such as the reasons for the development of Fujian maritime merchants, the trade engaged in by Fujian maritime merchants, the Fujian ships which played a decisive role in the overseas trade of Fujian maritime merchants.

Thermodynamic simulation and structural optimization of the collimator in the drift duct of EAST-NBI

Tang Ning,Hu Chun-dong,Xie Yuan-lai,Wei Jiang-long,Cui Zhi-Wei,Xie Jun-Wei,Pan Zhuo,Jiang Yao 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.11

The collimator is one of the high-heat-flux components used to avoid a series of vacuum and thermal problems. In this paper, the heat load distribution throughout the collimator is first calculated through experimental data, and a transient thermodynamic simulation analysis of the original model is carried out. The error of the pipe outlet temperature between the simulated and experimental values is 1.632%, indicating that the simulation result is reliable. Second, the model is optimized to improve the heat transfer performance of the collimator, including the contact mode between the pipe and the flange, the pipe material and the addition of a twisted tape in the pipe. It is concluded that the convective heat transfer coefficient of the optimized model is increased by 15.381% and the maximum wall temperature is reduced by 16.415%; thus, the heat transfer capacity of the optimized model is effectively improved. Third, to adapt the long-pulse steady-state operation of the experimental advanced superconducting Tokamak (EAST) in the future, steady-state simulations of the original and optimized collimators are carried out. The results show that the maximum temperature of the optimized model is reduced by 37.864% compared with that of the original model. The optimized model was changed as little as possible to obtain a better heat exchange structure on the premise of ensuring the consumption of the same mass flow rate of water so that the collimator can adapt to operational environments with higher heat fluxes and long pulses in the future. These research methods also provide a reference for the future design of components under high-energy and long-pulse operational conditions

Overexpression of Cyclooxygenase-1 Correlates with Poor Prognosis in Renal Cell Carcinoma

Yu, Zu-Hu,Zhang, Qiang,Wang, Ya-Dong,Chen, Jing,Jiang, Zhi-Mao,Shi, Min,Guo, Xin,Qin, Jie,Cui, Guang-Hui,Cai, Zhi-Ming,Gui, Yao-Ting,Lai, Yong-Qing Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

The aim of this study was to evaluate expression of COX-1 in renal cell carcinoma (RCC) and its prognostic value. mRNA of COX-1 was detected in 42 paired RCC and adjacent normal tissues with quantitative realtime polymerase chain reaction (qRT-PCR). Expression of COX-1 was also evaluated in 196 RCC sections and 91 adjacent normal tissues with immunohistochemistry. Statistical analysis was performed to assess COX-1 expression in RCC and its prognostic significance. The results of qRT-PCR showed mRNA levels of COX-1 in RCC tissues to be significantly higher than that in adjacent normal tissues (p < 0.001). Immunohistochemical assays also revealed COX-1 to be overexpressed in RCC tissues (p < 0.001). Statistical analysis demonstrated high expression of COX-1 was correlated with tumour size (p = 0.002), pathological stage (p = 0.003), TNM stage (p = 0.003, 0.007, 0.027, respectively), and tumour recurrence (p < 0.001). Survival analysis indicated patients with high expression of COX-1 had shorter survival time (p < 0.001), and COX-1 was an independent predictor. This is the first study to reveal overexpression of COX-1 in RRC and point to use as a prognostic marker in affected patients.

TSHR Variant Screening and Phenotype Analysis in 367 Chinese Patients With Congenital Hypothyroidism

Zhang Hai-Yang,Wu Feng-Yao,Li Xue-Song,Tu Ping-Hui,Zhang Cao-Xu,Yang Rui-Meng,Cui Ren-Jie,Wu Chen-Yang,Fang Ya,Yang Liu,Song Huai-Dong,Zhao Shuang-Xia 대한진단검사의학회 2024 Annals of Laboratory Medicine Vol.44 No.4

Background: Genetic defects in the human thyroid-stimulating hormone (TSH) receptor (TSHR) gene can cause congenital hypothyroidism (CH). However, the biological functions and comprehensive genotype–phenotype relationships for most TSHR variants associated with CH remain unexplored. We aimed to identify TSHR variants in Chinese patients with CH, analyze the functions of the variants, and explore the relationships between TSHR genotypes and clinical phenotypes. Methods: In total, 367 patients with CH were recruited for TSHR variant screening using whole-exome sequencing. The effects of the variants were evaluated by in-silico programs such as SIFT and polyphen2. Furthermore, these variants were transfected into 293T cells to detect their Gs/cyclic AMP and Gq/11 signaling activity. Results: Among the 367 patients with CH, 17 TSHR variants, including three novel variants, were identified in 45 patients, and 18 patients carried biallelic TSHR variants. In vitro experiments showed that 10 variants were associated with Gs/cyclic AMP and Gq/11 signaling pathway impairment to varying degrees. Patients with TSHR biallelic variants had lower serum TSH levels and higher free triiodothyronine and thyroxine levels at diagnosis than those with DUOX2 biallelic variants. Conclusions: We found a high frequency of TSHR variants in Chinese patients with CH (12.3%), and 4.9% of cases were caused by TSHR biallelic variants. Ten variants were identified as loss-of-function variants. The data suggest that the clinical phenotype of CH patients caused by TSHR biallelic variants is relatively mild. Our study expands the TSHR variant spectrum and provides further evidence for the elucidation of the genetic etiology of CH.

Optimal Two-Section Layouts for the Two-Dimensional Cutting Problem

( Jun Ji ),( Dun-hua Huang ),( Fei-fei Xing ),( Yao-dong Cui ) 한국정보처리학회 2021 Journal of information processing systems Vol.17 No.2

When generating layout schemes, both the material usage and practicality of the cutting process should be considered. This paper presents a two-section algorithm for generating guillotine-cutting schemes of rectangular blanks. It simplifies the cutting process by allowing only one size of blanks to appear in any rectangular block. The algorithm uses an implicit enumeration and a linear programming optimal cutting scheme to maximize the material usage. The algorithm was tested on some benchmark problems in the literature, and compared with the three types of layout scheme algorithm. The experimental results show that the algorithm is effective both in computation time and in material usage.