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        Prevention of Natural Flowering in Pineapple (Ananas comosus) by Shading and Urea Application

        Meng-Tzu Lin,Alfred Ming Chen1,Tzong-Shyan Lin,Ching-San Kuan,Ching-Lung Lee,Wen-Ju Yang 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.1

        The year-round production of pineapple (Ananas comosus var. comosus) is often interrupted by natural flowering during winter in Taiwan. A stable and promising technique for preventing natural flowering is required. In this study, we have tested the effect of shading and urea application on the flowering of pineapple. Shading 90% of sun light(s) before mid-November delayed the natural flowering of pineapple and the delay was affected by the plant age. For pineapples planted less than 11 month before mid-November, applying 1% urea plus shading treatment (SN) completely inhibited natural flowering. All the flowering-inhibited plants could be forced to flower by applying calcium carbide. Long-term shading might result in a decreased number of fruitlets within a fruit. However, 6 weeks of recovery before forcing flowering was sufficient for plants to produce fruits with quality equal to that of the control plants. In winter, pineapple plants that were prone to flowering tended to accumulate more leaf starch and increase their C/N ratio. The SN treatment increased the leaf nitrogen concentration and thus effectively maintained a low leaf C/N ratio. Furthermore, flowering-inhibited plants exhibited a constantly low level of leaf starch during the winter and their flowering forced by calcium carbide did not accompany with leaf starch accumulation.

      • Crack Detection for Metal Injection Molded Parts

        ( Hsing-meng Wang ),( Pei-hsuan Wu ),( Wen-tzong Lee ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

        In the research, image processing and machine learning techniques are employed on the surface defect detection of metal injection molded parts. There are two sections in this manuscript; one is image capturing system, and another one is the machine vision inspection system. For image capturing system, the ring light with diffuser is used, and it could project uniform light on the metal surface. CCD cameras are used to capture the images of metal injection molded parts. To obtain the best quality image, the inspection system uses Sobel operator searching for the focused position where the sharpness measure is maximized. For the machine vision inspection system, local feature information of the image is extracted by Histogram of oriented gradient, Gary level co-occurrence matrix and Local binary patterns. The selected features are imported into the support vector machines classification image to classify the crack defects.

      • SCIESCOPUSKCI등재

        Association of a Single Nucleotide Polymorphism in the 5'-Flanking Region of Porcine HSP70.2 with Backfat Thickness in Duroc Breed

        Chen, Ming-Yu,Huang, San-Yuan,Lin, En-Chung,Hseu, Tzong-Hsiung,Lee, Wen-Chuan Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.1

        Higher environmental temperature affects the economic performance of pigs. Heat shock protein 70 has been shown to play an important role in thermoresistance. The purpose of this study was to assess the effect of a single nucleotide polymorphism in the 5'-flanking region of porcine HSP70.2 on growth performance in Taiwanese Duroc. The genotype of this nt 393 polymorphic site could be verified by digestion with Bsa WI restriction enzyme of a PCR product. Pigs with TT and TC genotypes have thinner backfats than those with CC type (p<0.05). The result suggested that the polymorphic Bsa WI site in the 5'flanking region of porcine HSP70.2 may be used as a marker for the early selection of ultrasonic backfat thickness in Duroc pigs.

      • SCIESCOPUSKCI등재

        Augmentation of Thermotolerance in Primary Skin Fibroblasts from a Transgenic Pig Overexpressing the Porcine HSP70.2

        Chen, Ming-Yu,Tu, Ching-Fu,Huang, San-Yuan,Lin, Jyh-Hung,Tzang, Bor-Show,Hseu, Tzong-Hsiung,Lee, Wen-Chuan Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.1

        A high environmental temperature affects the economic performance of pigs. Heat shock protein 70 (HSP70) has been reported to participate importantly in thermotolerance. This study aims to produce transgenic pigs overexpressing porcine HSP70.2, the highly inducible one of HSP70 members, and to prove the cellular thermotolerance in the primary fibroblasts from the transgenics. A recombinant plasmid in which the sequence that encodes the porcine HSP70.2 gene is fused to green fluorescence protein (GFP) was constructed under the control of cytomegalovirus (CMV) enhancer and promoter. Two transgenic pigs were produced by microinjecting pCMV-HSP70-GFP DNA into the pronucleus of fertilized eggs. Immunoblot assay revealed the varied overexpression level (6.4% and 1.4%) of HSP70-GFP in transgenic pigs. After heating at $45^{\circ}C$ for 3 h, the survival rate (78.1%) of the primary fibroblast cells from the highly expressing transgenic pig exceeded that from the non-transgenic pig (62.9%). This result showed that primary fibroblasts overexpressing HSP70-GFP confer cell thermotolerance. We suggest that transgenic pigs overexpressing HSP70 might improve their thermotolerance in summer and therefore reduce the economic loss in animal production.

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