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Increased Sclerostin Levels after Further Ablation of Remnant Estrogen by Aromatase Inhibitors
김원진,Yoon Jung Chung,Se Hwa Kim,Sehee Park1,Jae Hyun Bae,Gyuri Kim1,Su Jin Lee1,Jo Eun Kim1,박병우,임승길,이유미 대한내분비학회 2015 Endocrinology and metabolism Vol.30 No.1
Background: Sclerostin is a secreted Wnt inhibitor produced almost exclusively by osteocytes, which inhibits bone formation. Aromatase inhibitors (AIs), which reduce the conversion of steroids to estrogen, are used to treat endocrine-responsive breast cancer. As AIs lower estrogen levels, they increase bone turnover and lower bone mass. We analyzed changes in serum sclerostin levels in Korean women with breast cancer who were treated with an AI. Methods: We included postmenopausal women with endocrine-responsive breast cancer (n=90; mean age, 57.7 years) treated with an AI, and compared them to healthy premenopausal women (n=36; mean age, 28.0 years). The subjects were randomly assigned to take either 5 mg alendronate with 0.5 μg calcitriol (n=46), or placebo (n=44) for 6 months. Results: Postmenopausal women with breast cancer had significantly higher sclerostin levels compared to those in premenopausal women (27.8±13.6 pmol/L vs. 23.1±4.8 pmol/L, P<0.05). Baseline sclerostin levels positively correlated with either lumbar spine or total hip bone mineral density only in postmenopausal women (r=0.218 and r=0.233; P<0.05, respectively). Serum sclerostin levels increased by 39.9%±10.2% 6 months after AI use in postmenopausal women; however, no difference was observed between the alendronate and placebo groups (39.9%±10.2% vs. 55.9%±9.13%, P>0.05). Conclusion: Serum sclerostin levels increased with absolute deficiency of residual estrogens in postmenopausal women with endocrine-responsive breast cancer who underwent AI therapy with concurrent bone loss.
Kim Jeong-Min,Kim Heui Man,Lee Eun Jung,Jo Hye Jun,Yoon Youngsil,Lee Nam-Joo,Son Junseock,Lee Ye-Ji,Kim Mi Seon,Lee Yong-Pyo,Chae Su-Jin,Park Kye Ryeong,Cho Seung-Rye,Park Sehee,Kim Su Jin,Wang Eunbye 질병관리본부 2020 Osong Public Health and Research Persptectives Vol.11 No.3
Objectives Coronavirus Disease-19 (COVID-19) is a respiratory infection characterized by the main symptoms of pneumonia and fever. It is caused by the novel coronavirus severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2), which is known to spread via respiratory droplets. We aimed to determine the rate and likelihood of SARS-CoV-2 transmission from COVID-19 patients through non-respiratory routes. Methods Serum, urine, and stool samples were collected from 74 hospitalized patients diagnosed with COVID-19 based on the detection of SARS-CoV-2 in respiratory samples. The SARS-CoV-2 RNA genome was extracted from each specimen and real-time reverse transcription polymerase chain reaction performed. CaCo-2 cells were inoculated with the specimens containing the SARS-COV-2 genome, and subcultured for virus isolation. After culturing, viral replication in the cell supernatant was assessed. Results Of the samples collected from 74 COVID-19 patients, SARS-CoV-2 was detected in 15 serum, urine, or stool samples. The virus detection rate in the serum, urine, and stool samples were 2.8% (9/323), 0.8% (2/247), and 10.1% (13/129), and the mean viral load was 1,210 ± 1,861, 79 ± 30, and 3,176 ± 7,208 copy/µL, respectively. However, the SARS-CoV-2 was not isolated by the culture method from the samples that tested positive for the SARS-CoV-2 gene. Conclusion While the virus remained detectable in the respiratory samples of COVID-19 patients for several days after hospitalization, its detection in the serum, urine, and stool samples was intermittent. Since the virus could not be isolated from the SARS-COV-2-positive samples, the risk of viral transmission via stool and urine is expected to be low.
Kim, Eunyoung,Kim, Sehee,Park, Yongsoon Informa UK Ltd. 2015 International journal of food sciences and nutriti Vol.66 No.3
<P>The purpose of this study is to investigate that sorghum extract (SE) exerts cholesterol-lowering effects through the regulation of hepatic cholesterol metabolism-related protein expression. C57BL/6 mice were fed a modified AIN-93G diet (NC) with saline, or a modified AIN-93G diet with 2% cholesterol and 0.25% cholic acid with either saline (HC) or 600 mg SE/kg body weight (HC-SE). Levels of total cholesterol and triglycerides in serum and liver were significantly lower in HC-SE than in HC. The expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase, sterol regulatory elementary binding protein2 and fatty acid synthase were significantly lower, whereas phosphorylated AMP-activated protein kinase expression was significantly higher in HC-SE than in HC. Cholesterol 7-α hydroxylase expression was also significantly higher in mice given SE than in those given HC. These results suggest that the cholesterol-lowering effect of SE may be related to the regulation of hepatic cholesterol metabolism in this mouse model.</P>
[PB-0036] Identification of genetic mutations in soybeans with low Kunitz Trypsin inhibitor activity
Sehee Kang(Sehee Kang),Seoyoung Shin(Seoyoung Shin),Euna Ko(Euna Ko),Woon Ji Kim(Woon Ji Kim),Byeong Hee Kang(Byeong Hee Kang),Chang Yeok Moon(Chang Yeok Moon),Yu Mi Choi(Yu Mi Choi),Bo-Keun Ha(Bo-Keu 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-
Studies on Genes and Biochemical Events Induced by Carbon Source Starvation in Plant Cells
Kim, Sehee,Lee, Sunmin,Lee, Taekkyun,Lee, Woosung 한국식물학회 1999 Journal of Plant Biology Vol.42 No.4
Carrot (Daucus carota L) suspension cells exhibit a number of physiological responses when carbon sources in the medium are depleted (i.e., carbon-source starvation). We previously reported that activities of several phospholipid catabolic enzymes, such as phospholipase D (PLD) and lipolytic acyl hydrolase (LAH), are induced to provide cells with alternative carbon sources. In this study we report sequence of PLD cDNA. When starvation was prolonged over approximately five days, cells started to die. To analyze the initiation of cell death, we examined the degradation of DNA and activity of DNA endonuclease. Preliminary results showed that DMA degradation occurred at the onset of cell death. Our findings suggest that carrot cells exhibit two different phases-acclimation response and cell death--during starvation. In working toward a long-term objective of understanding the whole scope of biochemical events during starvation, we have also catalogued the genes induced by starvation.
Kim, Sehee,Kim, Chanyang,Park, Seungjoon Journal of Endocrinology (Ltd. by Guarantee) 2016 Journal of molecular endocrinology Vol.57 No.3
<P>Adult hippocampal neurogenesis is decreased in type 2 diabetes, and this impairment appears to be important in cognitive dysfunction. Previous studies suggest that ghrelin gene products (acylated ghrelin (AG), unacylated ghrelin (UAG) and obestatin (OB)) promote neurogenesis. Therefore, we hypothesize that ghrelin gene products may reduce the harmful effects of high glucose (HG) on hippocampal neural stem cells (NSCs). The aim of this study was to investigate the role of these peptides on the survival of cultured hippocampal NSCs exposed to HG insult. Treatment of hippocampal NSCs with AG, UAG or OB inhibited HG-induced cell death and apoptosis. Exposure of cells to the growth hormone secretagogue receptor 1a antagonist abolished the protective effects of AG against HG toxicity, whereas those of UAG or OB were preserved. All three peptides attenuated HG-induced decrease in BrdU-labeled and phosphohistone-H3-labeled cells. We also investigated the effects of ghrelin gene products on the regulation of apoptosis at the mitochondrial level. AG, UAG or OB rescued hippocampal NSCs from HG insult by inhibiting intracellular and mitochondrial reactive oxygen species generation and stabilizing mitochondrial transmembrane potential. In addition, cells treated with ghrelin gene products showed an increased Bcl-2 and decreased Bax levels, thereby increasing the Bcl-2/Bax ratio, inhibiting cytochrome c release and preventing caspase-3 activation. Finally, AG-, UAG- or OB-mediated protection was dependent on the activities of adenosine monophosphate-activated protein kinase/uncoupling protein 2 pathway. Our data indicate that ghrelin gene products may act as survival factors that preserve mitochondrial function and inhibit oxidative stress-induced apoptosis.</P>
Kim, Sehee,Lee, Jihyun,Lee, Inah Frontiers Media S.A. 2012 Frontiers in Behavioral Neuroscience Vol.6 No.-
<P>The hippocampus is important for spatial navigation. Literature shows that allocentric visual contexts in the animal's background are critical for making conditional response selections during navigations. In a traditional maze task, however, it is difficult to identify exactly which subsets of visual contexts are critically used. In the current study, we tested in rats whether making conditional response selections required the hippocampus when using computer-generated visual contextual stimuli in the animal's background as in primate and human studies. We designed a new task, visual contextual response selection (VCRS) task, in which the rat ran along a linear track and encountered a touchscreen monitor at the end of the track. The rat was required to touch one of the adjacent rectangular box images depending on the visual contextual stimuli displayed in the two peripheral monitors positioned on both sides of the center touchscreen monitor. The rats with a GABA-A receptor agonist, muscimol (MUS), infused bilaterally in the dorsal hippocampi showed severe performance deficits in the VCRS task and the impairment was completely reversible with vehicle injections. The impairment in contextual response selection with hippocampal inactivations occurred regardless of whether the visual context was presented in the side monitors or in the center touchscreen monitor. However, when the same visual contextual stimuli were pitted against each other between the two side monitors and as the rats simply ran toward the visual context associated with reward on a T-shaped track, hippocampal inactivations with MUS showed minimal disruptions, if any, in performance. Our results suggest that the hippocampus is critically involved in conditional response selection using visual stimuli in the background, but it is not required for the perceptual discrimination of those stimuli.</P>