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임준범 ( Joon Bum Lim ),이수범 ( Soo Beom Lee ),성정곤 ( Jung Gon Sung ),박준태 ( Jun Tae Park ) 한국안전학회(구 한국산업안전학회) 2012 한국안전학회지 Vol.27 No.4
This research has analyzed factors affecting customers` satisfaction when they use parking lots of big retailers so that, in case of constructing, operating or managing such retailers, proper measures can be come up with. The analysis has been implemented on the basis of survey, and influence factors including entry/exit lamp, parking cars`` traffic flow, parking type, pedestrians`` movement after parking and safety. Parking lot users`` satisfaction has been analyzed by using the Structural Equation Modelling and, as a result of it, in case of a detached or single building, pedestrians movement flow after parking contributed the most to the users`` satisfaction, while, in case of multipurpose complex, parking cars traffic flow has the most influential factor. It is interpreted that users of a single building put a bigger emphasis on entry to the shop and their way back to the parked area after their shopping while customers of multipurpose complex might have various purposes of visiting the place and there are relatively more cars so that they put more emphasis on traffic counterflow, other cars and pedestrians.
Sang-Hoon Park,Mi-Ran Lee,Tae-Suk Kim,Sang-Ki Baek,Sang-Jin Jin,Jin-Wook Kim,Sang-Gon Jeon,Ho-Baek Yoon,Joon-Hee Lee 한국동물생명공학회(구 한국동물번식학회) 2014 Reproductive & developmental biology Vol.38 No.4
Differentiated nuclei can experimentally be returned to an undifferentiated embryonic status after nuclear transfer (NT) to unfertilized metaphase II (MII) oocytes. Nuclear reprogramming is triggered immediately after somatic cell nucleus transfer (SCNT) into recipient cytoplasm and this period is regarded as a key stage for optimizing reprogramming. In a recent study (Dai et al., 2010), use of m-carboxycinnamic acid bishydroxamide (CBHA) as a histone deacetylase inhibitor during the in vitro early culture of murine cloned embryos modifies the acetylation status of somatic nuclei and increases the developmental competence of SCNT embryos. Thus, we examined the effects of CBHA treatment on the in vitro preimplantation development of porcine SCNT embryos and on the acetylated status of histone H3K9 on cloned embryos at the zygote stage. We performed the three groups SCNT: SCNT (NT), CBHA treatment at the porcine fetus fibroblast cells (PFFs) used as donor cells prior to SCNT (CBHA-C) and CBHA treatment at the porcine SCNT embryos during the in vitro early culture after oocyte activation (CBHA-Z). The PFFs were treated with a 15 μM of CBHA (8 h) for the early culture and the porcine cloned embryos were treated with a 100 μM concentration of CBHA during the in vitro early culture (10 h). Cleavage rates and development to the blastocyst stage were assessed. No significant difference was observed the cleavage rate among the groups (82.6%, 76.4% and 82.2%, respectively). However, the development competence to the blastocyst stage was significantly increased in CBHA-Z embryos (22.7%) as compared to SCNT and CBHA-C embryos (8.6% and 4.1%)(p<0.05). Total cell numbers and viable cell numbers at the blastocyst stage of porcine SCNT embryos were increased in CBHA-Z embryos as compared to those in CBHA-C embryos (p<0.05). Signal level of histone acetylation (H3K9ac) at the zygote stage of SCNT was increased in CBHA-Z embryos as compared to SCNT and CBHA-C embryos. The results of the present study suggested that treatment with CBHA during the in vitro early culture (10 h) had significantly increased the developmental competence and histone acetylation level at the zygote stage.
Physiologically based pharmacokinetic (PBPK) modeling of fl urbiprofen in different CYP2C9 genotypes
Sang-Sup Whang,Chang-Keun Cho,Eui Hyun Jung,Pureum Kang,Hye-Jung Park,Yun Jeong Lee,Chang-Ik Choi,Jung-Woo Bae,Hyung Sik Kim,Choon-Gon Jang,Seok-Yong Lee 대한약학회 2022 Archives of Pharmacal Research Vol.45 No.8
The aim of this study was to establish the physiologicallybased pharmacokinetic (PBPK) model of fl urbiprofenrelated to CYP2C9 genetic polymorphism anddescribe the pharmacokinetics of fl urbiprofen in diff erentCYP2C9 genotypes. PK-Sim® software was used for themodel development and validation. A total of 16 clinicalpharmacokinetic data for fl urbiprofen in diff erent CYP2C9genotypes, dose regimens, and age groups were used for thePBPK modeling. Turnover number (k cat ) of CYP2C9 valueswere optimized to capture the observed profi les in diff erentCYP2C9 genotypes. In the simulation, predicted fractionmetabolized by CYP2C9, fraction excreted to urine, bioavailability,and volume of distribution were similar to previouslyreported values. Predicted plasma concentration-timeprofi les in diff erent CYP2C9 genotypes were visually similarto the observed profi les. Predicted AUC inf in CYP2C9*1/*2 ,CYP2C9*1/*3 , and CYP2C9*3/*3 genotypes were 1.44-,2.05-, and 3.67-fold higher than the CYP2C9*1/*1 genotype. The ranges of fold errors for AUC inf , C max , and t 1/2 were 0.84–1.00, 0.61–1.22, and 0.74–0.94 in development and0.59–0.98, 0.52–0.97, and 0.61–1.52 in validation, respectively,which were within the acceptance criterion. Thus, thePBPK model was successfully established and described thepharmacokinetics of fl urbiprofen in diff erent CYP2C9 genotypes,dose regimens, and age groups. The present modelcould guide the decision-making of tailored drug administrationstrategy by predicting the pharmacokinetics of fl urbiprofenin various clinical scenarios.
Park, Jae-Hyun,Yan, Yi-Dong,Chi, Sang-Cheol,Hwang, Doo Hyung,Shanmugam, Srinivasan,Lyoo, Won Seok,Woo, Jong Soo,Yong, Chul Soon,Choi, Han-Gon Pharmaceutical Society of Great Britain 2011 Journal of pharmacy and pharmacology Vol.63 No.4
<P>Objectives??To avoid the major adverse effects induced by Cremophor EL formulated in the commercial paclitaxel products of Taxol. Methods??An injectable paclitaxel solid dispersion free of Cremophor was prepared by a supercritical antisolvent process and then was fully characterized and investigated with regard to its short-term and long-term stability. Pharmacokinetics in rats was also evaluated compared with the commercial product. Key findings??The solid dispersion system at a 1/20/40 weight ratio of paclitaxel/HP-관-CD/HCO-40 had a paclitaxel solubility of about 10?g/ml, an almost 10??00-fold increase over its aqueous solubility. This system was physically stable for at least six months or four weeks in accelerated conditions (40??2°C; RH: 75??5%) and stress conditions (60°C), respectively. The precipitation time of paclitaxel solid dispersion in 0.9% sodium chloride injection at a concentration of 1000?/ml was above 70? at room temperature. Intravenous administration of paclitaxel solid dispersion at a dose of 6?g/kg revealed no significant differences when compared with the commercial product. However, our results obtained at a dose of 12?g/kg showed a striking non-linear increase in the plasma Cmax and AUCall with increased dose. In addition, the concentrations of paclitaxel in various organs in the solid dispersion group were found to be higher than those of Taxol at 6?g/kg, and the paclitaxel levels in these organs increased proportionately with increasing dose. Conclusions??Nano-scale paclitaxel solid dispersion without Cremophor EL provided advantageous results over Taxol with respect to the physicochemical properties, safety, clinic convenience and pharmacokinetic behaviour in rats.</P>
Park, Jongho,Lee, Kimoon,Lee, Seung Yong,Nandadasa, Chandani N.,Kim, Sungho,Lee, Kyu Hyoung,Lee, Young Hee,Hosono, Hideo,Kim, Seong-Gon,Kim, Sung Wng American Chemical Society 2017 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.139 No.2
<P>We have synthesized a single crystalline Y2C electride of centimeter-scale by floating-zone method and successfully characterized its anisotropic electrical and magnetic properties. In-plane resistivity upturn at low temperature together with anisotropic behavior of negative magnetoresistance is ascribed to the stronger suppression of spin fluctuation along in-plane than that along the c-axis, verifying the existence of magnetic moments preferred for the c-axis. A superior magnetic moment along the c-axis to that along the in-plane direction strongly demonstrates the anisotropic magnetism of Y2C electride containing a magnetically easy axis. It is clarified from the theoretical calculations that the anisotropic nature of the Y2C electride originates from strongly localized anionic electrons with an inherent magnetic anisotropy in the interlayer spaces.</P>
Park, Sang-Hoon,Lee, Mi-Ran,Kim, Tae-Suk,Baek, Sang-Ki,Jin, Sang-Jin,Kim, Jin-Wook,Jeon, Sang-Gon,Yoon, Ho-Baek,Lee, Joon-Hee The Korean Society of Animal Reproduction 2014 Reproductive & developmental biology Vol.38 No.4
Differentiated nuclei can experimentally be returned to an undifferentiated embryonic status after nuclear transfer (NT) to unfertilized metaphase II (MII) oocytes. Nuclear reprogramming is triggered immediately after somatic cell nucleus transfer (SCNT) into recipient cytoplasm and this period is regarded as a key stage for optimizing reprogramming. In a recent study (Dai et al., 2010), use of m-carboxycinnamic acid bishydroxamide (CBHA) as a histone deacetylase inhibitor during the in vitro early culture of murine cloned embryos modifies the acetylation status of somatic nuclei and increases the developmental competence of SCNT embryos. Thus, we examined the effects of CBHA treatment on the in vitro preimplantation development of porcine SCNT embryos and on the acetylated status of histone H3K9 on cloned embryos at the zygote stage. We performed the three groups SCNT: SCNT (NT), CBHA treatment at the porcine fetus fibroblast cells (PFFs) used as donor cells prior to SCNT (CBHA-C) and CBHA treatment at the porcine SCNT embryos during the in vitro early culture after oocyte activation (CBHA-Z). The PFFs were treated with a $15{\mu}M$ of CBHA (8 h) for the early culture and the porcine cloned embryos were treated with a $100{\mu}M$ concentration of CBHA during the in vitro early culture (10 h). Cleavage rates and development to the blastocyst stage were assessed. No significant difference was observed the cleavage rate among the groups (82.6%, 76.4% and 82.2%, respectively). However, the development competence to the blastocyst stage was significantly increased in CBHA-Z embryos (22.7%) as compared to SCNT and CBHA-C embryos (8.6% and 4.1%)(p<0.05). Total cell numbers and viable cell numbers at the blastocyst stage of porcine SCNT embryos were increased in CBHA-Z embryos as compared to those in CBHA-C embryos (p<0.05). Signal level of histone acetylation (H3K9ac) at the zygote stage of SCNT was increased in CBHA-Z embryos as compared to SCNT and CBHA-C embryos. The results of the present study suggested that treatment with CBHA during the in vitro early culture (10 h) had significantly increased the developmental competence and histone acetylation level at the zygote stage.
Park, Yeon-Sun,Lee, Sang-Rok,Kim, Young-Gon The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.1
We developed an mPCR assay for the simultaneous detection, in one tube, of Escherichia coli O157:H7, Salmonella spp., Staphylococcus aureus and Listeria monocytogenes using species-specific primers. The mPCR employed the E. coli O157:H7 specific primer Stx2A, Salmonella spp. specific primer Its, S. aureus specific primer Cap8A-B and L. monocytogenes specific primer Hly. Amplification with these primers produced products of 553, 312, 405 and 210 bp, respectively. All PCR products were easily detected by agarose gel electrophoresis, and the sequences of the specific amplicons assessed. Potential pathogenic bacteria, in laboratory-prepared and four commercially available kimchi products, were using this mPCR assay, and the amplicons cloned and sequenced. The results correlated exactly with sequences derived for amplicons obtained during preliminry tests with known organisms. The sensitivity of the assay was determined for the purified pathogen DNAs from four strains. The mPCR detected pathogen DNA at concentrations ranging from approximately 0.45 to $0.05\;pM/{\mu}l$. Thus, this mPCR assay may allow for the rapid, reliable and cost-effective identification of four potentially pathogens present in the mixed bacterial communities of commercially available kimchi.