http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Mardina, Primata,Li, Jinglin,Patel, Sanjay K.S.,Kim, In-Won,Lee, Jung-Kul,Selvaraj, Chandrabose The Korean Society for Microbiology and Biotechnol 2016 Journal of microbiology and biotechnology Vol.26 No.7
Methanol is a versatile compound that can be biologically synthesized from methane (CH<sub>4</sub>) by methanotrophs using a low energy-consuming and environment-friendly process. Methylocella tundrae is a type II methanotroph that can utilize CH<sub>4</sub> as a carbon and energy source. Methanol is produced in the first step of the metabolic pathway of methanotrophs and is further oxidized into formaldehyde. Several parameters must be optimized to achieve high methanol production. In this study, we optimized the production conditions and process parameters for methanol production. The optimum incubation time, substrate, pH, agitation rate, temperature, phosphate buffer and sodium formate concentration, and cell concentration were determined to be 24 h, 50% CH<sub>4</sub>, pH 7, 150 rpm, 30℃, 100 mM and 50 mM, and 18 mg/ml, respectively. The optimization of these parameters significantly improved methanol production from 0.66 to 5.18 mM. The use of alginate-encapsulated cells resulted in enhanced methanol production stability and reusability of cells after five cycles of reuse under batch culture conditions.
( Primata Mardina ),( Jinglin Li ),( Sanjay K. S. Patel ),( In-won Kim ),( Jung-kul Lee ),( Chandrabose Selvaraj ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6
Methanol is a versatile compound that can be biologically synthesized from methane (CH4) by methanotrophs using a low energy-consuming and environment-friendly process. Methylocella tundrae is a type II methanotroph that can utilize CH4 as a carbon and energy source. Methanol is produced in the first step of the metabolic pathway of methanotrophs and is further oxidized into formaldehyde. Several parameters must be optimized to achieve high methanol production. In this study, we optimized the production conditions and process parameters for methanol production. The optimum incubation time, substrate, pH, agitation rate, temperature, phosphate buffer and sodium formate concentration, and cell concentration were determined to be 24 h, 50% CH4, pH 7, 150 rpm, 30°C, 100 mM and 50 mM, and 18 mg/ml, respectively. The optimization of these parameters significantly improved methanol production from 0.66 to 5.18 mM. The use of alginate-encapsulated cells resulted in enhanced methanol production stability and reusability of cells after five cycles of reuse under batch culture conditions.
Biological Methanol Production by a Type II Methanotroph Methylocystis bryophila
( Sanjay K. S. Patel ),( Primata Mardina ),( Sang-yong Kim ),( Jung-kul Lee ),( In-won Kim ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4
Methane (CH4) is the most abundant component in natural gas. To reduce its harmful environmental effect as a greenhouse gas, CH4 can be utilized as a low-cost feed for the synthesis of methanol by methanotrophs. In this study, several methanotrophs were examined for their ability to produce methanol from CH4; including Methylocella silvestris, Methylocystis bryophila, Methyloferula stellata, and Methylomonas methanica. Among these methanotrophs, M. bryophila exhibited the highest methanol production. The optimum process parameters aided in significant enhancement of methanol production up to 4.63 mM. Maximum methanol production was observed at pH 6.8, 30°C, 175 rpm, 100 mM phosphate buffer, 50 mM MgCl2 as a methanol dehydrogenase inhibitor, 50% CH4 concentration, 24 h of incubation, and 9 mg of dry cell mass ml-1 inoculum load, respectively. Optimization of the process parameters, screening of methanol dehydrogenase inhibitors, and supplementation with formate resulted in significant improvements in methanol production using M. bryophila. This report suggests, for the first time, the potential of using M. bryophila for industrial methanol production from CH4.
Patel, Sanjay K.S.,Kumar, Virendra,Mardina, Primata,Li, Jinglin,Lestari, Rowina,Kalia, Vipin C.,Lee, Jung-Kul Elsevier 2018 Bioresource technology Vol.263 No.-
<P><B>Abstract</B></P> <P>In the present study, co-cultures of the methanotrophs <I>Methylocella tundrae</I>, <I>Methyloferula stellata</I>, and <I>Methylomonas methanica</I> were evaluated for improving methanol production with their application. Among the different combinations, the co-culture of <I>M. tundrae</I> and <I>M. methanica</I> increased methanol production to 4.87 mM using methane (CH<SUB>4</SUB>) as feed. When simulated biogas mixtures were used as feed, the maximum methanol production was improved to 8.66, 8.45, and 9.65 mM by free and encapsulated co-cultures in 2% alginate and silica-gel, respectively. Under repeated batch conditions, free and immobilized co-cultures using alginate and silica-gel resulted in high cumulative production, up to 24.43, 35.95, and 47.35 mM, using simulated biohythane (CH<SUB>4</SUB> and hydrogen), respectively. This is the first report of methanol production from defined free and immobilized co-cultures using simulated biogas mixtures as feed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The co-culture of <I>Methanotrophs</I> is effective to produce methanol using simulated biogases. </LI> <LI> The silica-gel based encapsulated co-culture is an effective approach for methanol production. </LI> <LI> This is the first report of methanol production from immobilized co-cultures using biogas. </LI> </UL> </P>