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Genetic Toxicity Test of Methylcarbamate by Ames, Micronucleus, Comet Assays and Microarray Analysis
Kwon, Kyoung-J.,Go, Seo-Y.,Park, Sue-N.,Sheen, Yhun-Y. The Korean Society of Applied Pharmacology 2007 Biomolecules & Therapeutics(구 응용약물학회지) Vol. No.
Carbamates have excellent insecticidal activities against a broad spectrum of insects. They possess knocking-down, fast-killing, and systemic effects, however, they are toxic to mammals. In this study, we have carried out in vitro genetic toxicity test of methylcarbamate and microarray analysis of differentially expressed genes in response to methylcarbamate. Methylcarbamate did not show mutations in base substitution strain TA1535 both with and without exogenous metabolic activation. Methylcarbamate did not show mutations in frame shift TA98 both with and without exogenous metabolic activation. Methylcarbamate showed DNA damage based on single cell gel/comet assay in L5178Y cells both with and without exogenous metabolic activation. Methylcarbamate did not increase micronuclei in CHO cells both with and without exogenous metabolic activation. Microarray analysis of gene expression profiles in L5178Y cells in response to methylcarbamate selected differentially expressed 132 genes that could be candidate biomarkers of genetic toxic action of methylcarbamate.
Genetic Toxicity Test of Methylcarbamate by Ames, Micronucleus, Comet Assays and Microarray Analysis
( Kyoung J. Kwon ),( Seo Y. Go ),( Sue N. Park ),( Yhun Y. Sheen ) 한국응용약물학회 2007 Biomolecules & Therapeutics(구 응용약물학회지) Vol.15 No.3
Carbamates have excellent insecticidal activities against a broad spectrum of insects. They possess knocking-down, fast-killing, and systemic effects, however, they are toxic to mammals. In this study, we have carried out in vitro genetic toxicity test of methylcarbamate and microarray analysis of differentially expressed genes in response to methylcarbamate. Methylcarbamate did not show mutations in base substitution strain TA1535 both with and without exogenous metabolic activation. Methylcarbamate did not show mutations in frame shift TA98 both with and without exogenous metabolic activation. Methylcarbamate showed DNA damage based on single cell gel/comet assay in L5178Y cells both with and without exogenous metabolic activation. Methylcarbamate did not increase micronuclei in CHO cells both with and without exogenous metabolic activation. Microarray analysis of gene expression profiles in L5178Y cells in response to methylcarbamate selected differentially expressed 132 genes that could be candidate biomarkers of genetic toxic action of methylcarbamate.
Period-2 spiral waves supported by nonmonotonic wave dispersion.
Kwon, Okyu,Kim, Tae Yun,Lee, Kyoung J Published by the American Physical Society through 2010 Physical review. E, Statistical, nonlinear, and so Vol.82 No.4
<P>Rotating spiral waves appear ubiquitously in a wide range of nonlinear systems, and they play important roles in many biological phenomena. Recently, unusual spiral waves, which support period-2 dynamics, have been found in several different systems including cardiac tissues as well as nonlinear chemical reaction-diffusion systems. They are potentially significant as an intermediate dynamic state linking regularly rotating period-1 spiral waves to complex dynamic states such as cardiac fibrillations; for example, it is intrinsic of period-2 spiral waves to have 'line defects' and their instability can lead to a spatiotemporal chaos. Previous mathematical models regarding period-2 spiral waves are mostly based on a coupled system of period-2 oscillators, but these are inappropriate for the description of a large class of systems that are composed of (nonoscillatory) excitable elements-a good example being the heart. In this paper we hypothesize that excitable media, which support a nonmonotonic conduction velocity dispersion relation, can sustain period-2 oscillatory spiral waves. We explicitly demonstrate that the new mechanism can create period-2 spirals by computer simulations on a simple mathematical model describing spiral wave front dynamics.</P>
Lee, Young-Kyoung,Lim, Jin J.,Jeoun, Un-woo,Min, Seongki,Lee, Eun-beom,Kwon, So Mee,Lee, Changhan,Yoon, Gyesoon American Society for Biochemistry and Molecular Bi 2017 The Journal of biological chemistry Vol.292 No.49
<P>Impaired mitochondrial oxidative phosphorylation (OXPHOS) capacity, accompanied by enhanced glycolysis, is a key metabolic feature of cancer cells, but its underlying mechanism remains unclear. Previously, we reported that human hepatoma cells that harbor OXPHOS defects exhibit high tumor cell invasiveness via elevated claudin-1 (CLN1). In the present study, we show that OXPHOS-defective hepatoma cells (SNU354 and SNU423 cell lines) exhibit reduced expression of mitochondrial ribosomal protein L13 (MRPL13), a mitochondrial ribosome (mitoribosome) subunit, suggesting a ribosomal defect. Specific inhibition of mitoribosomal translation by doxycycline, chloramphenicol, or siRNA-mediated MRPL13 knockdown decreased mitochondrial protein expression, reduced oxygen consumption rate, and increased CLN1-mediated tumor cell invasiveness in SNU387 cells, which have active mitochondria. Interestingly, we also found that exogenous lactate treatment suppressed MRPL13 expression and oxygen consumption rate and induced CLN1 expression. A bioinformatic analysis of the open RNA-Seq database from The Cancer Genome Atlas (TCGA) liver hepatocellular carcinoma (LIHC) cohort revealed a significant negative correlation between MRPL13 and CLN1 expression. Moreover, in patients with low MRPL13 expression, two oxidative metabolic indicators, pyruvate dehydrogenase B expression and the ratio of lactate dehydrogenase type B to type A, significantly and negatively correlated with CLN1 expression, indicating that the combination of elevated glycolysis and deficient MRPL13 activity was closely linked to CLN1-mediated tumor activity in LIHC. These results suggest that OXPHOS defects may be initiated and propagated by lactate-mediated mitoribosomal deficiencies and that these deficiencies are critically involved in LIHC development.</P>
적색 및 청색 수지 첨가 피복재가 토마토와 고추의 생육에 미치는 영향
권준국(Joon Kook Kwon),조명환(Myeomg Whan Cho),강윤임(Yun Im Kang),박경섭(Kyoung Sub Park),우종규(J.G. Woo) (사)한국생물환경조절학회 2010 생물환경조절학회지 Vol.19 No.4
플라스틱하우스 피복재의 광 이용효율을 높이기 위해 제조과정에 적색, 청색, 적색+청색 수지를 추가로 첨가하여 제조한 필름을 피복한 시설 내에서 토마토와 고추를 재배하였으며 시설 내 광 환경 변화와 작물의 생육, 수량 등을 조사하였다. 대체로 일반필름에 비하여 적색 및 청색 수지를 첨가한 필름에서 광합성유효복사의 투과량이 5~6% 많았고, 300~1,100㎚의 광투과율은 0.5~1.0% 높게 나타났다. 피복재 중에는 적색+청색 혼합수지 첨가필름에서 토마토와 고추의 건물중이 높게 나타났고 일반필름에 비해 과실수량이 15~20% 많았으며 과실 당도도 약 0.5°Bx 높았다. Spectral irradiance of greenhouse covering films that three resins (red, blue, red plus blue) were added to get higher utilization efficiency of sunlight were compared to the normal film in this study. Growth and yield of tomato and pepper grown under the films were also investigated. Transmittance of PAR (photosynthetically active radiation, 400~700 ㎚) and sunlight (300~1,100 ㎚) of red or blue resin-added films increased by 5 to 6% and 0.5 to 1.0%, respectively. As compared to the normal film, fruit yield and soluble solid content of tomato and pepper grown under red plus blue resin-added film increased by 15 to 20% and by 0.5°Bx.
( Seo Y. Go ),( Kyoung J. Kwon ),( Sue N. Park ),( Yhun Y. Sheen ) 한국응용약물학회 2007 Biomolecules & Therapeutics(구 응용약물학회지) Vol.15 No.3
Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a major constituent of rhubarb. Although it has been claimed to have a wild spectrum of therapeutic value, its side effects, especially in human kidney cells have not been well characterized. In this study, we have carried out in vitro genetic toxicity test of emodin and microarray analysis of differentially expressed genes in response to emodin. The result of Ames test showed mutations with emodin treatment in base substitution strain TA1535 both with and without exogenous metabolic activation. Likewise, emodin showed mutations in frame shift TA98 both with and without exogenous metabolic activation. The result of COMET assay in L5178Y cells with emodin treatment showed DNA damage both with and without exogenous metabolic activation. Emodin did not increase micronuclei in CHO cells both with and without exogenous metabolic activation. 150 Genes were selected as differentially expressed genes in response to emodin by microarray analysis and these genes would be candidate biomarkers of genetic toxic action of emodin.
Go, Seo-Y.,Kwon, Kyoung-J.,Park, Sue-N.,Sheen, Yhun-Y. The Korean Society of Applied Pharmacology 2007 Biomolecules & Therapeutics(구 응용약물학회지) Vol. No.
Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a major constituent of rhubarb. Although it has been claimed to have a wild spectrum of therapeutic value, its side effects, especially in human kidney cells have not been well characterized. In this study, we have carried out in vitro genetic toxicity test of emodin and microarray analysis of differentially expressed genes in response to emodin. The result of Ames test showed mutations with emodin treatment in base substitution strain TA1535 both with and without exogenous metabolic activation. Likewise, emodin showed mutations in frame shift TA98 both with and without exogenous metabolic activation. The result of COMET assay in L5178Y cells with emodin treatment showed DNA damage both with and without exogenous metabolic activation. Emodin did not increase micronuclei in CHO cells both with and without exogenous metabolic activation. 150 Genes were selected as differentially expressed genes in response to emodin by microarray analysis and these genes would be candidate biomarkers of genetic toxic action of emodin.
Choi, Seong Ho,Park, Sung Kwon,Choi, Chang Weon,Li, Xiang Zi,Kim, Kyoung Hoon,Kim, Won Young,Jeong, Joon,Johnson, Bradley J.,Zan, Linsen,Smith, Stephen B. Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.3
We hypothesized that supplementing finishing diets with palm oil would promote adipogenic gene expression and stearoyl-CoA desaturase (SCD) gene expression in subcutaneous (s.c.) and intramuscular (i.m.) adipose tissues of feedlot steers. Eighteen Angus and Angus crossbred steers were assigned to three groups of 6 steers and fed a basal diet (control), with 3% palm oil, or with 3% soybean oil, for 70 d, top-dressed daily. Tailhead s.c. adipose tissue was obtained by biopsy at 14 d before the initiation of dietary treatments and at 35 d of dietary treatments. At slaughter, after 70 d of dietary treatment, tailhead s.c. adipose tissue and i.m. adipose tissue were obtained from the longissimus thoracis muscle. Palm oil increased plasma palmitic acid and soybean oil increased plasma linoleic acid and ${\alpha}$-linolenic acid relative to the initial sampling time. Expression of AMP-activated protein kinase alpha ($AMPK{\alpha}$) and peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) increased between the initial and intermediate biopsies and declined thereafter (p<0.03). SCD gene expression did not change between the initial and intermediate biopsies but declined by over 75% by the final period (p = 0.04), and G-coupled protein receptor 43 (GPR43) gene expression was unaffected by diet or time on trial. Soybean oil decreased (p = 0.01) $PPAR{\gamma}$ gene expression at the intermediate sample time. At the terminal sample time, $PPAR{\gamma}$ and SCD gene expression was less in i.m. adipose tissue than in s.c. adipose tissue (p<0.05). $AMPK{\alpha}$ gene expression was less in s.c. adipose tissue of palm oil-fed steers than in control steers (p = 0.04) and CCAAT enhancer binding protein-beta ($CEBP{\beta}$) gene expression was less in s.c. and i.m. adipose tissues of palm oil-fed steers than in soybean oil-fed steers (p<0.03). Soybean oil decreased SCD gene expression in s.c. adipose tissue (p = 0.05); SCD gene expression in palm oil-fed steers was intermediate between control and soybean oil-fed steers. Contrary to our original hypothesis, palm oil did not promote adipogenic gene expression in s.c. and i.m. adipose tissue.
제왕절개술 후 복벽반흔에 발생한 자궁내막증 환자에서 mesh를 이용한 복부근막재건술
김수미 ( Su Mi Kim ),김현경 ( Hyun Kyoung Kim ),남궁정 ( Jung Nam Kung ),송재연 ( Jae Yeon Song ),정인철 ( In Chol Jung ),조현희 ( Hyun Hee Cho ),권동진 ( Dong Jin Kwon ),유영옥 ( Young Oak Lew ),김은중 ( Eun Jung Kim ),김장흡 ( J 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.7
Endometriosis is defined by the presence of endometrial glands and stroma outside uterine cavity showing variable clinical symptoms under effect of estrogen. The occurrence rate of abdominal wall endometriosis after cesarean section is rare making diagnosis difficult. The case of a 36-year-old female presenting with the very painful mass and pain arising in the caesarean section scar is reported. The curable treatment of abdominal wall endometriosis is the surgical removal of all the pathological tissue, through a large excision. The involved fascia was widely excised and the abdominal wall was reconstructed using polypropylene mesh.