Detection of drug-resistant viruses using bionanotechnology

Juyeon JUNG 한국생물공학회 2016 한국생물공학회 학술대회 Vol.2016 No.10

Practical application of DNA markers for high-throughput authentication of Panax ginseng and Panax quinquefolius from commercial ginseng products

Jung, Juyeon,Kim, Kyung Hee,Yang, Kiwoung,Bang, Kyong-Hwan,Yang, Tae-Jin The Korean Society of Ginseng 2014 Journal of Ginseng Research Vol.38 No.2

Korean ginseng (Panax ginseng) and American ginseng (Panax quinquefolius) are widely used medicinal plants with similar morphology but different medicinal efficacy. Roots, flowers, and processed products of Korean and American ginseng can be difficult to differentiate from each other, leading to illegal trade in which one species is sold as the other. This study was carried out to develop convenient and reliable chloroplast genome-derived DNA markers for authentication of Korean and American ginseng in commercial processed products. One codominant marker could reproducibly identify both species and intentional mixtures of the two species. We further developed a set of species-unique dominant DNA markers. Each species-specific dominant marker could detect 1% cross contamination with other species by low resolution agarose gel electrophoresis or quantitative polymerase chain reaction. Both markers were successfully applied to evaluate the original species from various processed ginseng products purchased from markets in Korea and China. We believe that high-throughput application of this marker system will eradicate illegal trade and promote confident marketing for both species to increase the value of Korean as well as American ginseng in Korea and worldwide.

Practical application of DNA markers for high-throughput authentication of Panax ginseng and Panax quinquefolius from commercial ginseng products

Juyeon Jung,김경희,양기웅,방경환,양태진 고려인삼학회 2014 Journal of Ginseng Research Vol.38 No.2

Korean ginseng (Panax ginseng) and American ginseng (Panax quinquefolius) are widely used medicinalplants with similar morphology but different medicinal efficacy. Roots, flowers, and processed productsof Korean and American ginseng can be difficult to differentiate from each other, leading to illegal tradein which one species is sold as the other. This study was carried out to develop convenient and reliablechloroplast genome-derived DNA markers for authentication of Korean and American ginseng in commercialprocessed products. One codominant marker could reproducibly identify both species andintentional mixtures of the two species. We further developed a set of species-unique dominant DNAmarkers. Each species-specific dominant marker could detect 1% cross contamination with other speciesby low resolution agarose gel electrophoresis or quantitative polymerase chain reaction. Both markerswere successfully applied to evaluate the original species from various processed ginseng productspurchased from markets in Korea and China. We believe that high-throughput application of this markersystem will eradicate illegal trade and promote confident marketing for both species to increase thevalue of Korean as well as American ginseng in Korea and worldwide.

Photo-triggered fluorescent labelling of recombinant proteins in live cells

Jung, Deokho,Sato, Kohei,Min, Kyoungmi,Shigenaga, Akira,Jung, Juyeon,Otaka, Akira,Kwon, Youngeun The Royal Society of Chemistry 2015 Chemical communications Vol.51 No.47

<P>A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.</P> <P>Graphic Abstract</P><P>A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed for background-free fluorescent labelling of target proteins with the necessary spatiotemporal control. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5cc01067e'> </P>

New kid on the wall: Oxidative barrier in skin barrier functions

( Juyeon Jung ),( Sekyoo Jeong ) 한국피부장벽학회 2023 한국피부장벽학회지 Vol.25 No.2

산소 호흡을 통해 에너지를 생산하는 생물체에 있어 산화 스트레스는 불가피한 부산물이라 할 수 있다. 산화 스트레스의 주요 생성물인 활성산소종 (Reactive oxygen species: ROS) 은 세포와 조직에 다양한 악영향을 미치는 것 외에도, 전자 수용체로서 필수적인 역할도 담당하고 있다. Lipoxygenase (LOX) 와 cyclooxygenase (COX) 은 산소를 요구하는 효소로서 불포화지방산(polyunsaturated fatty acid: PUFA)에서 oxylipin 이라고 불리는 지질 대사체를 만들어내는 역할을 한다. 자외선 조사 또는 오염 물질 노출 등을 포함한 외부 스트레스 또한 세포와 조직에서 산화 스트레스를 유발하는 것으로 알려져 있다. 피부의 다양한 장벽 기능 중, 표피 투과 장벽은 구조적, 생물학적, 물리적 관계를 통해 피부의 다른 장벽 기능과 연관되어 있으며, 인체의 생존에 핵심적인 역할을 담당하고 있다. 항산화 장벽 기능은 피부의 주요한 장벽 기능 중 하나로 인식되고 있으나, 표피 투과 장벽이나 면역 장벽을 포함한 다른 장벽 기능과의 상관관계에 대한 연구는 미흡한 실정이다. Autosomal recessive congenital ichthyosis (ARCI) 질환의 유전적 소인에 대한 연구와 유전자 조작 동물 모델을 이용한 연구 결과, 표피에서 발현되는 LOX (epidermis-specific LOXs: eLOXs) 가 omega-hydroxy 세라마이드와 각질세포외막 (cornified envelope: CE) 단백질 간의 결합에 관여함으로써 각질세포지질외막 (cornified lipid envelope: CLE) 형성에 핵심적인 역할을 한다는 것이 보고된 바 있다. 최근 이러한 eLOXs의 활성이 thymic-stromal lymphopoietin (TSLP)를 비롯한 염증성 사이토카인에 의해 조절된다는 것이 알려지면서, 표피 투과 장벽 - 면역 장벽 - 항산화 장벽 간의 상호 관계에 대한 근거가 제시된 바 있다. 본 종설에서는 피부 항산화 장벽의 주요 구성 요소와 이들의 역할, 그리고 다른 장벽 기능과의 상호 작용 등에 대해 논의하고자 한다.

Chemical biology-based approaches on fluorescent labeling of proteins in live cells

Jung, Deokho,Min, Kyoungmi,Jung, Juyeon,Jang, Wonhee,Kwon, Youngeun The Royal Society of Chemistry 2013 Molecular bioSystems Vol.9 No.5

<P>Recently, significant advances have been made in live cell imaging owing to the rapid development of selective labeling of proteins <I>in vivo</I>. Green fluorescent protein (GFP) was the first example of fluorescent reporters genetically introduced to protein of interest (POI). While GFP and various types of engineered fluorescent proteins (FPs) have been actively used for live cell imaging for many years, the size and the limited windows of fluorescent spectra of GFP and its variants set limits on possible applications. In order to complement FP-based labeling methods, alternative approaches that allow incorporation of synthetic fluorescent probes to target POIs were developed. Synthetic fluorescent probes are smaller than fluorescent proteins, often have improved photochemical properties, and offer a larger variety of colors. These synthetic probes can be introduced to POIs selectively by numerous approaches that can be largely categorized into chemical recognition-based labeling, which utilizes metal-chelating peptide tags and fluorophore-carrying metal complexes, and biological recognition-based labeling, such as (1) specific non-covalent binding between an enzyme tag and its fluorophore-carrying substrate, (2) self-modification of protein tags using substrate variants conjugated to fluorophores, (3) enzymatic reaction to generate a covalent binding between a small molecule substrate and a peptide tag, and (4) split-intein-based C-terminal labeling of target proteins. The chemical recognition-based labeling reaction often suffers from compromised selectivity of metal–ligand interaction in the cytosolic environment, consequently producing high background signals. Use of protein–substrate interactions or enzyme-mediated reactions generally shows improved specificity but each method has its limitations. Some examples are the presence of large linker protein, restriction on the choice of introducible probes due to the substrate specificity of enzymes, and competitive reaction mediated by an endogenous analogue of the introduced protein tag. These limitations have been addressed, in part, by the split-intein-based labeling approach, which introduces fluorescent probes with a minimal size (∼4 amino acids) peptide tag. In this review, the advantages and the limitations of each labeling method are discussed.</P> <P>Graphic Abstract</P><P>This review summarizes various approaches to incorporate synthetic fluorescent probes to target proteins in live cells as well as current efforts to control the fluorescent signal using external stimuli. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2mb25422k'> </P>

Identification of quantitative trait loci for fusarium wilt resistance in radish (Raphanus sativus)

Juyeon Jung,Jaehwang Ryu,Yeonok Choi,Young-Pyo Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Radish, Raphanus sativus(2n = 18), belonging to the brassicaceae family, is herbaceous plant with 1-2 years life cycle. It is cultivated worldwide for producing leafy and root vegetables. Although an economically important crop, the genetics of yield and quality traits, disease resistance are not well-studies. The major purpose of this project is development of molecular breeding technology in radish. In this project, quantitative trait loci (QTL) for Fusarium wilt resistance of radish were analyzed. To identify QTL, genetic linkage map of radish was constructed using F2 mapping population derived from a cross between two inbred lines, “DB01” (resistant) and “DB05” (susceptible). A total 319 markers have been mapped into nine linkage groups, covering 639.3cM with an average distance of 2cM between loci. QTL mapping detected 2 loci conferring Fusarium wilt resistance. Two QTLs were located on LG3 and LG7, respectively. The QTL of LG3, flanked by EAGGMCT6 and WALK500 marker, exhibited a LOD value ranging from 2.3 to 8.7, and the R2 (Phenotypic variations) ranging from 28 to 48% in four tests. This QTL was named qYR1. The QTL of LG7, flanked by EACCMCAC-202 and DCJ14-390 marker, exhibited a LOD value ranging from 6.2 to 10.6, and the R2 ranging from 42 to 55% in four tests. This QTL was named qYR2. The results of the QTL analysis may be useful in marker-assisted selection (MAS) of Fusarium wilt resistant radish cultivars.

Cosmetics as A Defender for Skin Barrier Function

( Juyeon Jung ),정세규 ( Sekyoo Jeong ) 한국피부장벽학회 2020 한국피부장벽학회지 Vol.22 No.1

As the front line of protecting the human body against outer environment, stratum corneum (SC) is relentlessly attacked by the external stressors. Along with natural stressors, such as low humidity, abrupt temperature change, and sunlight, various artificial stressors also impact the skin barrier function. While the cosmetics are one of the most common “stressors” possibly disrupting epidermal permeability barrier function, potential beneficial effects of cosmetics on skin barrier function are also repeatedly reported. Supplementation of SC intercellular lipids with barrier creams, which show either compositional or structural similarity to those of SC lipids, can accelerate the recovery of damaged barrier functions. Bioactive ingredients stimulating the epidermal sphingolipids synthesis can also improve the skin barrier functions. Anti-oxidants and anti-inflammatory molecules, either directly or indirectly, protect skin barrier function against external stressors. In this review, protecting effects of cosmetics on skin barrier function and their possible defending mechanisms will be briefly discussed. An ideal combination of “barrier cosmetics” will be also suggested.

Cosmetics as a Defender for Skin Barrier Function

( Juyeon Jung ),( Sekyoo Jeong ) 한국피부장벽학회 2020 한국피부장벽학회지 Vol.22 No.1

As the front line of protecting the human body against outer environment, stratum corneum (SC) is relentlessly attacked by the external stressors. Along with natural stressors, such as low humidity, abrupt temperature change, and sunlight, various artificial stressors also impact the skin barrier function. While the cosmetics are one of the most common “stressors” possibly disrupting the epidermal permeability barrier function, potential beneficial effects of cosmetics on skin barrier function are also repeatedly reported. Supplementation of SC intercellular lipids with barrier creams, which show either compositional or structural similarity to those of SC lipids, can accelerate the recovery of damaged barrier functions. Bioactive ingredients stimulating the epidermal sphingolipids synthesis can also improve the skin barrier functions. Anti-oxidants and anti-inflammatory molecules, either directly or indirectly, protect skin barrier function against external stressors. In this review, protecting effects of cosmetics on skin barrier function and their possible defending mechanisms will be briefly discussed. An ideal combination of “barrier cosmetics” will be also suggested.

독립어의 범주적 특징과 그 유형

정주연(Jung, Juyeon) 형태론 2019 형태론 Vol.21 No.1

이 글에서는 독립어의 특징을 살펴보고 그 범주의 범위를 명확히 하며 일정한 기준에 따라 해당 범주의 유형을 나누었다. 이 글은 아직 독립어의 범주가 명확하지 않다는 것을 전제로 하여 논의를 시작하고 있다. 그렇기 때문에 단순히 기존에 독립어로 다루어지던 것들만 살펴보는 것이 아니라 독립어의 범주에서 다루는 데에 다소 논란이 되었던 것들까지 모두 고려되어야 할 것이다. 문장 성분의 하나인 독립어는 다른 문장 성분으로 간주될 수 없는 문장 내의 요소이다. 이는 문장 내에 분명하게 존재하는 어떤 요소가 다른 문장 성분들이 가지는 주요한 특징을 가지지 않는다는 것을 밝힘으로써 확인될 수 있을 것이다. 이런 점에서 ‘어떤 특징을 가지고 있지 않음’ 자체가 독립어의 가장 큰 특징이 된다. 그리고 이를 바탕으로 하여 독립어의 범위를 정할 수 있으며, 여러 가지 기준을 적용하여 그 유형을 보임말, 부름말, 느낌 말, 응답말, 디딤말로 구분할 수 있다. The purpose of this study is to identify the morphological and syntactic features of the independent words, and to divide the types of the independent words based on them. Because the category of the independent words is not clear yet, it is necessary not only to look at what was previously treated as an independent words but also to consider some controversy in subclassification of the independent words. The independent words, which is one of the sentence components, is an element in the sentence that can not be regarded as another sentence components. This can be confirmed by clarifying that certain elements that are clearly present in a sentence do not have the key features of the other sentence components. Based on these features, the range of the independent words can be determined. And we will classify the types of independent words by applying various criteria.