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Woo, Seon Rang,Ham, Yunhee,Kang, Wonyoung,Yang, Heekyoung,Kim, Sujong,Jin, Juyoun,Joo, Kyeung Min,Nam, Do-Hyun Hindawi Publishing Corporation 2014 BioMed research international Vol.2014 No.-
<P>Standard treatment for glioblastoma comprises surgical resection, chemotherapy with temozolomide, and radiotherapy. Nevertheless, majority of glioblastoma patients have recurrence from resistance to the cytotoxic conventional therapies. We examined combinational effects of KML001, an arsenic compound targeting telomeres of chromosomes with temozolomide or irradiation, in glioblastoma cell lines and xenograft models, to overcome the therapeutic limitation of chemoradiation therapy for glioblastoma. Although KML001 alone showed little effects on <I>in vitro</I> survival of glioblastoma cells, cell death by <I>in vitro</I> temozolomide treatment or irradiation was synergistically potentiated by combination with KML001. Since phosphorylated <I>γ</I>-H2AX, cleaved casepase-3, and cleaved PARP were dramatically increased by KML001, the synergistic effects would be mediated by increased DNA damage and subsequent tumor cell apoptosis. Combinatorial effects of KML001 were observed not only in chemo- and radiosensitive glioblastoma cell line, U87MG, but also in the resistant cell line, U251MG. In the U87MG glioblastoma xenograft models, KML001 did not have systemic toxicity but showed synergistic therapeutic effects in combination with temozolomide or irradiation to reduce tumor volumes significantly. These data indicated that KML001 could be a candidate sensitizer to potentiate therapeutic effects of conventional cytotoxic treatment for glioblastoma.</P>
Shim, Joo-Hyun,Park, Mi-Rung,Yang, Byoung-Chul,Ko, Yeoung-Gyu,Oh, Keon-Bong,Lee, Jeong-Woong,Woo, Jae-Seok,Park, Eung-Woo,Park, Soo-Bong,Hwang, Seong-Soo The Korean Society of Animal Reproduction 2009 Reproductive & developmental biology Vol.33 No.3
This study was performed to comprehend the developmental characteristics of cloned embryos knocked out (KO) of $\alpha$-1,3-galactosyltransferase (GalT) gene. Immature oocytes were collected and cultured for 40 hrs (1-step) or 20hrs (with hormone) + 20hrs (without hormone) (2-step). The embryos transferred with miniature pig ear fibroblast cell were used as control. The reconstructed embryos were cultured in PZM-3 with 5% $CO_2$ in air at $38.5^{\circ}C$ for 6 days. To determine the quality of the blstocysts, TUNEL and quantitative realtime RT-PCR were performed. The embryos were transferred to a surrogate (Landrace) at an earlier stage of the estrus cycle. The maturation rate was significantly higher in 2-step method than that of 1-step (p<0.05). The blastocyst development of GalT KO embryos was significantly lower than that of normal cloned embryos (p<0.05). The total and apoptotic cell number of GalT KO blastocysts was not different statistically from control. The relative abundance of Bax-$\alpha$/Bcl-xl ratio was significantly higher in both cloned blastocysts than that of in vivo blastocysts (p<0.05). Taken together, it can be postulated that the lower developmental potential and higher expression of apoptosis related genes in GalT KO SCNT embryos might be a cause of a low efficiency of GalT KO cloned miniature pig production.
( Seon Rang Woo ),( Jeong Eun Park ),( Yang Hyun Kim ),( Yeun Jin Ju ),( Hyun Jin Shin ),( Hyun Yoo Joo ),( Eun Ran Park ),( Sung Hee Hong ),( Gil Hong Park ),( Kee Ho Lee ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.12
The synthetic machinery of ATF4 (activating transcription factor 4) is activated in response to various stress conditions involved in nutrient restriction, endoplasmic reticulum homeostasis, and oxidation. Stress-induced inhibition of proteasome activity triggers the unfolded protein response and endoplasmic reticulum stress, where ATF4 is crucial for consequent biological events. In the current study, we showed that the NAD+-dependent deacetylase, SIRT1, suppresses ATF4 synthesis during proteasome inhibition. SIRT1 depletion via transfection of specific siRNA into HeLa cells resulted in a significant increase in ATF4 protein, which was observed specifically in the presence of the proteasome inhibitor MG132. Consistent with SIRT1 depletion data, transient transfection of cells with SIRT1-overexpressing plasmid induced a decrease in the ATF4 protein level in the presence of MG132. Interestingly, however, ATF4 mRNA was not affected by SIRT1, even in the presence of MG132, indicating that SIRT1-induced suppression of ATF4 synthesis occurs under post-transcriptional control. Accordingly, we propose that SIRT1 serves as a negative regulator of ATF4 protein synthesis at the post-transcriptional level, which is observed during stress conditions, such as proteasome inhibition.
Ah Rang Cho(조아랑),Jin Kyung Park(박진경),Jong Woo Kim(김종우),Ji Young Song(송지영),Joo Ho Chung(정주호),Geon Ho Bahn(반건호) 대한생물치료정신의학회 2007 생물치료정신의학 Vol.13 No.1
목적 : Dopamine beta-hydroxylase(DBH) 유전자는 정신병적 증상과 관련하여 정신분열병의 후보유전자 중의 하나이다. 본 연구는 DBH 유전자 내 새로운 단일염기다형성의 유전자형을 확인하고 정신분열병과의 유전적 연관성을 알아보고자 하였다. 연구대상 및 방법 : DSM-Ⅳ에 의해 정신분열병으로 진단된 입원환자 89명과 정신질환의 과거력과 가족력이 없는 대조군 109명을 대상으로 하였다. DBH 유전자의 네 번째 엑손 부위를 다중효소중합연쇄반응(Polymerase Chain Reaction, PCR)으로 증폭하여 DNA sequencing 기법으로 유전자형을 확인하고 연관성을 알아보기 위한 통계 분석을 시행하였다. 결과 : DBH 유전자 내 4개의 단일염기다형성(DBH5818G/A, DBH5990G/A, DBH6153C/T, DBH6259A/G)을 확인하였다. 이 4개의 단일염기다형성 모두에서 정신분열병 환자군과 정상 대조군 간 유전자형 분포와 대립유전자 빈도 및 일배체형 빈도에서의 유의한 차이는 없었다. DBH와의 관련성이 보고된 양성 정신병적 증상이 두드러지는 편집형 정신분열병 환자군과 정상 대조군 간 일배체형 빈도에서도 유의한 차이는 보이지 않았다. 결론 : DBH5818G/A, DBH5990G/A, DBH6153C/T, 그리고 DBH6259A/G 다형성은 한국 정신분열병 환자군과 유전적 관련성이 없을 것으로 추정된다.
GM-CSF Induces Inflammatory Macrophages by Regulating Glycolysis and Lipid Metabolism
Na, Yi Rang,Gu, Gyo Jung,Jung, Daun,Kim, Young Won,Na, Juri,Woo, Jin Sun,Cho, Joo Youn,Youn, Hyewon,Seok, Seung Hyeok The American Association of Immunologists, Inc. 2016 JOURNAL OF IMMUNOLOGY Vol.197 No.10
<P>GM-CSF induces proinflammatory macrophages, but the underlying mechanisms have not been studied thus far. In this study, we investigated the mechanisms of how GM-CSF induces inflammatory macrophages. First, we observed that GM-CSF increased the extent of LPS-induced acute glycolysis in murine bone marrow-derived macrophages. This directly correlates with an inflammatory phenotype because glycolysis inhibition by 2-deoxyglucose abolished GM-CSF-mediated increase of TNF-alpha,IL-1 beta, IL-6, and IL-12p70 synthesis upon LPS stimulation. Increased glycolytic capacity is due to de novo synthesis of glucose transporter ( GLUT)-1,-3, and-4, as well as c-myc. Mean while, GM-CSF increased 3-hydroxy-3-methyl-glutaryl-CoA reductase, which is the rate-limiting enzyme of the mevalonate pathway. Inhibition of acute glycolysis or 3-hydroxy-3-methyl-glutaryl-CoA reductase abrogated the inflammatory effects of GM-CSF priming in macrophages. Finally, mice with inflamed colons exposed to dextran sodium sulfate containing GLUT-1(high) macrophages led to massive uptake of [F-18]-fluorodeoxyglucose, but GM-CSF neutralization reduced the positron-emission tomography signal in the intestine and also decreased GLUT-1 expression in colonic macrophages. Collectively, our results reveal glycolysis and lipid metabolism created by GM-CSF as the underlying metabolic constructs for the function of inflammatory macrophages.</P>
Stress-induced domain dynamics and phase transitions in epitaxially grown VO<sub>2</sub> nanowires
Sohn, Jung Inn,Joo, Heung Jin,Kim, Keun Soo,Yang, Hyoung Woo,Jang, A-Rang,Ahn, Docheon,Lee, Hyun Hwi,Cha, SeungNam,Kang, Dae Joon,Kim, Jong Min,Welland, Mark E IOP Pub 2012 Nanotechnology Vol.23 No.20
<P>We demonstrate that surface stresses in epitaxially grown VO<SUB>2</SUB> nanowires (NWs) have a strong effect on the appearance and stability of intermediate insulating M<SUB>2</SUB> phases, as well as the spatial distribution of insulating and metallic domains during structural phase transitions. During the transition from an insulating M<SUB>1</SUB> phase to a metallic R phase, the coexistence of insulating M<SUB>1</SUB> and M<SUB>2</SUB> phases with the absence of a metallic R phase was observed at atmospheric pressure. In addition, we show that, for a VO<SUB>2</SUB> NW without the presence of an epitaxial interface, surface stresses dominantly lead to spatially inhomogeneous phase transitions between insulating and metallic phases. In contrast, for a VO<SUB>2</SUB> NW with the presence of an epitaxial interface, the strong epitaxial interface interaction leads to additional stresses resulting in uniformly alternating insulating and metallic domains along the NW length.</P>
Fungal proliferation and calcium accumulation in the orange slime of Cornus controversa
Park, Junhyung,Kwon, Jun Hyeong,Kim, Hae Rang,Kwon, Ohkyung,Koh, Sang-Hyun,Kim, Pan-Gi,Bae, Kwan Ho,Kim, Dong Geun,Kwon, Oh Kyu,Joo, Sung Hyun,Jung, Sung-Gwan,Kim, Ki Woo Sage Publishing 2017 Forest Science And Technology Vol.13 No.4