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( Sung-soo Park ),( Seul Ki Lim ),( Jieun Lee ),( Hyo Kyeong Park ),( Min-sung Kwon ),( Misun Yun ),( Namhee Kim ),( Young Joon Oh ),( Hak-jong Choi ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.11
Obesity and related metabolic diseases are major problems worldwide. Some probiotics are currently considered potential therapeutic strategies for obesity. We aimed to investigate the anti-obesity efficacy of Latilactobacillus sakei WIKIM31 in obese mice induced by a high fat diet. The administration of a high-fat diet with L. sakei WIKIM31 reduced body weight gain, epididymal fat mass, triglyceride and total cholesterol levels in the blood, and remarkably decreased the expression of lipogenesis-related genes in the epididymal adipose tissue and liver. Interestingly, intake of L. sakei WIKIM31 improved gut barrier function by increasing the gene expression of tight junction proteins and suppressing the inflammatory responses. Additionally, L. sakei WIKIM31 enhanced the production of short-chain fatty acids, such as butyrate and propionate, in the intestinal tract. These results showed that L. sakei WIKIM31 can be used as a potential therapeutic probiotic for obesity.
Kim, Jieun,Kim, Su Mi,Seo, Jeong Eun,Choi, Min Gew,Lee, Jun Ho,Sohn, Tae Sung,Kim, Sung,Bae, Jae Moon,Seo, Seong Il The Korean Gastric Cancer Association 2014 Journal of gastric cancer Vol.14 No.3
We report our experience of a concurrent robot assisted distal gastrectomy and partial nephrectomy for synchronous early gastric cancer and renal cell carcinoma. A 55-year-old female patient was diagnosed with early gastric cancer on screening endoscopy. Abdominal computed tomography showed an incidental right renal cell carcinoma. Robot assisted distal gastrectomy was performed, followed by partial nephrectomy. The final pathological examination showed signet ring cell carcinoma within the lamina propria and renal cell carcinoma with negative resection margins. The patient showed no evidence of recurrence at 6-months. A robot-assisted combined operation could be a treatment option for early stages of synchronous malignancies.
Direct-Write Patterning of Bacterial Cells by Dip-Pen Nanolithography
Kim, Jieun,Shin, Young-Hun,Yun, Seong-Hun,Choi, Dong-Sik,Nam, Ji-Hye,Kim, Sung Ryong,Moon, Sung-Kwon,Chung, Bong Hyun,Lee, Jae-Hyuck,Kim, Jae-Ho,Kim, Ki-Young,Kim, Kyung-Min,Lim, Jung-Hyurk American Chemical Society 2012 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.134 No.40
<P>The ability of dip-pen nanolithography (DPN) to generate nano- or microarrays of soft or hard materials (e.g., small molecules, DNA, proteins, nanoparticles, sols, and polymers) in a direct-write manner has been widely demonstrated. The transporting of large-sized ink materials such as bacteria, however, remains a significant challenge with this technique. The size limitation of the water meniscus formed between the DPN tip and the solid surface becomes a bottleneck in such diffusion-based molecular transport experiments. Herein, we report a straightforward “stamp-on” DPN method that uses a nanostructured poly(2-methyl-2-oxazoline) hydrogel-coated tip and carrier agents to generate patterns of micrometer-sized Escherichia coli JM 109 bacterial cells. We demonstrate that this approach enables the deposition of a single bacterial cell array on a solid surface or arrays of layers of multiple cells by modulating the viscosity of the “ink” solution. Fluorescence microscopy images indicated that the deposited bacterial cells were kept alive on Luria–Bertani-agar layered solid surfaces after DPN patterning.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2012/jacsat.2012.134.issue-40/ja3073808/production/images/medium/ja-2012-073808_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja3073808'>ACS Electronic Supporting Info</A></P>
Lee, Jieun,Cho, Sung-Min,Kim, Min Sung,Lee, Sug Hyung,Chung, Yeun-Jun,Jung, Seung-Hyun Korea Genome Organization 2017 Genomics & informatics Vol.15 No.1
Tumor tissues from biopsies or surgery are major sources for the next generation sequencing (NGS) study, but these procedures are invasive and have limitation to overcome intratumor heterogeneity. Recent studies have shown that driver alterations in tumor tissues can be detected by liquid biopsy which is a less invasive technique capable of both capturing the tumor heterogeneity and overcoming the difficulty in tissue sampling. However, it is still unclear whether the driver alterations in liquid biopsy can be detected by targeted NGS and how those related to the tissue biopsy. In this study, we performed whole-exome sequencing for a breast cancer tissue and identified PTEN p.H259fs*7 frameshift mutation. In the plasma DNA (liquid biopsy) analysis by targeted NGS, the same variant initially identified in the tumor tissue was also detected with low variant allele frequency. This mutation was subsequently validated by digital polymerase chain reaction in liquid biopsy. Our result confirm that driver alterations identified in the tumor tissue were detected in liquid biopsy by targeted NGS as well, and suggest that a higher depth of sequencing coverage is needed for detection of genomic alterations in a liquid biopsy.
Choi, Jieun,Kim, Man-Ho,Han, Jun Young,Chae, Ji Eon,Lee, Won Hee,Lee, Young Moo,Lee, So Young,Jang, Jong Hyun,Kim, Jin Young,Henkensmeier, Dirk,Yoo, Sung Jong,Sung, Yung-Eun,Kim, Hyoung-Juhn Elsevier 2018 Journal of membrane science Vol.568 No.-
<P><B>Abstract</B></P> <P>In this report, we introduced spirobiindane group to poly(arylene ether sulfone) (PES) to build the structure of polymers with intrinsic microporosity (PIMs). A novel PESs (QOH-SBIs), which have spirobiindane and tetra(quaternary ammonium) hydroxide pendant moieties, were synthesized for anion-conducting binder material in membrane electrode assembly (MEA) of solid alkaline exchange membrane fuel cell (SAEMFC). The time-lag method was used to check the high gas permeability of the polymers. The high permeability is due to the micro-pores at the molecular level that is formed by the difference in chain thicknesses between two alternating units, thick spirobiindane group and thin arylene ether sulfone group. QOH-SBIs shows a semi-rigid chain conformation in a solution. The inter-chain spacing and chain conformation were measured with wide angle X-ray diffraction (WAXD) and small angle neutron scattering (SANS), respectively. High gas permeability directly affected the performance of SAEMFC. The MEA with spirobiindane-modified PES shows much higher maximum power density than that of spirobiindane-free PESs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Spiro moieties were introduced to PESs to design polymers of intrinsic microporosity. </LI> <LI> Difference in chain thickness create microporous channels for gas diffusion. </LI> <LI> Inter-chain spacing and chain conformation were calculated from with WAXD and SANS. </LI> <LI> Enhancing gas permeability of the electrode binder improved the cell performance. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
( Han-sung Kwon ),( Seo-yeon Kim ),( Jieun Park ),( Han-sung Hwang ),( In-sook Sohn ) 대한주산의학회 2017 Perinatology Vol.28 No.4
Objective: To compare perinatal outcomes of gestational diabetic women with a family history of type 2 diabetes mellitus (DM), who were diagnosed through early or late screening. Methods: After 2010, women with a family history of DM underwent 2-step screening at the initial visit, mostly before 16 weeks of gestation. The perinatal outcomes were compared with those of historical cohort screened at 24-28 weeks of gestation between 2005 and 2009. The primary outcomes were complications associated with maternal hyperglycemia such as primary cesarean delivery, large for gestational age (LGA), neonatal hypoglycemia, and fetal anomaly. Results: The risk of gestational diabetes mellitus (GDM) was 20.8% (67/322) in women with a history of DM in a first-degree relative. Women who were screened before 16 weeks of gestation were more likely to have a high level of hemoglobin A1C at diagnosis and receive insulin therapy for glycemic control than the Late-screen group. But odds ratios of LGA, primary cesarean delivery and fetal anomalies compared with normal control were highest in the Late-screen group than in the Early screen group and the Low risk GDM group. Conclusion: Some perinatal outcomes may be more favorable in women with GDM and a family history of DM who were screened before 16 weeks of gestation rather than routinely.
Cho, Sung-Yup,Chae, Jeesoo,Na, Deukchae,Kang, Wonyoung,Lee, Ahra,Min, Seoyeon,Kang, Jinjoo,Choi, Boram,Lee, Jieun,Sung, Chang Ohk,Chuang, Jeffrey H.,Lee, Charles,Lee, Won-Suk,Park, Hansoo,Kim, Jong-Il American Association for Cancer Research 2019 Clinical Cancer Research Vol.25 No.9
<P><B>Purpose:</B></P><P>Genomic and transcriptomic alterations during metastasis are considered to affect clinical outcome of colorectal cancers, but detailed clinical implications of metastatic alterations are not fully uncovered. We aimed to investigate the effect of metastatic evolution on <I>in vivo</I> treatment outcome, and identify genomic and transcriptomic alterations associated with drug responsiveness.</P><P><B>Experimental Design:</B></P><P>We developed and analyzed patient-derived xenograft (PDX) models from 35 patients with colorectal cancer including 5 patients with multiple organ metastases (MOMs). We performed whole-exome, DNA methylation, and RNA sequencing for patient and PDX tumors. With samples from patients with MOMs, we conducted phylogenetic and subclonal analysis and <I>in vivo</I> drug efficacy test on the corresponding PDX models.</P><P><B>Results:</B></P><P>Phylogenetic analysis using mutation, expression, and DNA methylation data in patients with MOMs showed that mutational alterations were closely connected with transcriptomic and epigenomic changes during the tumor evolution. Subclonal analysis revealed that initial primary tumors with larger number of subclones exhibited more dynamic changes in subclonal architecture according to metastasis, and loco-regional and distant metastases occurred in a parallel or independent fashion. The PDX models from MOMs demonstrated therapeutic heterogeneity for targeted treatment, due to subclonal acquisition of additional mutations or transcriptomic activation of bypass signaling pathway during tumor evolution.</P><P><B>Conclusions:</B></P><P>This study demonstrated <I>in vivo</I> therapeutic heterogeneity of colorectal cancers using PDX models, and suggests that acquired subclonal alterations in mutations or gene expression profiles during tumor metastatic processes can be associated with the development of drug resistance and therapeutic heterogeneity of colorectal cancers.</P>
초등과학교사연구회 활동이 교사 전문성에 미치는 영향에 대한 교사의 인식
장지은 ( Jieun Sung Chang ),성승민 ( Seung Min Sung ),여상인 ( Sang Ihn Yeo ) 경북대학교 과학교육연구소 2014 科學敎育硏究誌 Vol.38 No.3
본 연구에서는 초등과학교사연구회 활동이 교사 전문성에 미치는 영향에 대한 교사의 인식을 알아보기 위해 연구회의 운영 및 활동내용, 참여 동기는 무엇인지와 교사 전문성 신장에 대한 인식은 어떠한지 등을 살펴보았다. 연구대상은 경기도에 소재하고 있는 N 초등과학교사연구회와 소속된 교사이다. 자료는 참여관찰과 면담을 통해 수집하였고 수집된 자료의 종류는 참여관찰록, 연구회 활동사진, 문서자료, 면담자료 등이었다. 참여관찰을 하면서 본 연구회에 참여하고 있는 교사들의 인식을 면밀하게 살펴보기 위해서 연구회 참여 경력을 기준으로 5명의 교사를 선정하여 연구회 참여 동기, 개인적인 만족도, 전문성 신장에 대한 교사의 인식 등에 관한 심층면담을 실시하였다. 연구 결과, N 초등과학교사연구회는 운영 방식과 활동 내용에 관하여 회원들 간의 지속적인 협의를 통해 운영 체제를 확립해가고 있으며, 초등학생들의 수준에 맞는 과학 수업을 위해 고민하는 모습을 볼 수 있었다. 대부분의 교사가 과학 및 과학 교수법에 대한 관심과 열의로 연구회에 참여하게 되었으나, 연구회에 대한 만족도는 개인별로 차이가 있음을 확인할 수 있었다. 그러나 교사들 모두 연구회가 교사로서의 전문성 신장에 도움이 된다고 생각하고 있었다. 본 연구 결과를 바탕으로 더 발전적인 초등과학교사연구회가 운영되기 위한 교육적 시사점을 얻고자 하였다. The purpose of this study was to investigate how the activity in the elementary school science teachers’ study group help teachers improve their professionalism and what the motivation was for the continuous participation by analyzing the operation system of study group and the teachers`` perception. For this study, the N Elementary Science Teachers’ Study Group in Gyeong-gi province was chosen, which has been maintained for a long time. Data such as observation journals, contents of activities, and some documents from this study group were collected through the participant observation methodology, and the perceptions about the participating motivation, the personal satisfaction and the impact on the professionalism were investigated by interviewing 5 teachers who participating in this study group. The results of this study were as follows: First, voluntary participation activated the teachers’ study group, so teachers who belong to the study group, had a great satisfaction in the activities of the study group. Second, the more teachers participated in study group, they thought their professionalism has been improved. Based on the result of this study, implications to improve the professionalism of teachers were suggested.