괴화 추출물이 대식세포에서의 nitric oxide와 interleukin-6의 생성에 미치는 영향

이지은,이주연,최점일,김종관,김성조,Lee, Ji-Eun,Lee, Ju-Youn,Choi, Jeom-Il,Kim, Chong-Kwan,Kim, Sung-Jo 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.1

Both nitric oxide (NO) and interleukin-6 (IL-6) have been thought to have a role in the pathogenesis of inflammatory periodontal disease as it does in other inflammatory diseases, and the inhibitors of NO and IL-6 production have been considered as potential anti-inflammatory agents. In this study, we evaluated methanol extract of Sophorae Flos for inhibition of NO and IL-6 production in Prevotella intermedia LPS-induced mouse macrophages RAW264.7 cells. Dried Sopharae Flos was sliced, and extracted with 100% methanol. LPS from p. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supematants and IL-6 was measured using mouse IL-6 ELISA kit. Western blot analysis of iNOS and analysis of reverse transcription (RT)-PCR products were carried out. The methanol extract of Sophorae Flos concentration-dependently reduced the production of NO and the expression of iNOS protein and mRNA in RAw264.7 cells treated with P. intermedia LPS. Sophorae Flos also suppressed IL-6 production and the expression of IL-6 mRNA in RAw264.7 cells stimulated by P. intermedia LPS. The inhibition of NO and IL-6 production by Sophorae Flos may be useful in the therapy of inflammatory diseases such as periodontitis. This hypothesis, however, remains to be tested.

Identification of immunological parameters associated with the alveolar bone level in periodontal patients

Park, Chang-Seo,Lee, Ju-Yeon,Kim, Sung-Jo,Choi, Jeom-Il Korean Academy of Periodontology 2010 Journal of Periodontal & Implant Science Vol.40 No.2

Purpose: The present study was performed to clarify the relationship between periodontal disease severity and selected immunological parameters consisting of serum IgG titer against periodontopathogenic bacteria, the expression of the helper T-cell cytokine by gingival mononuclear cells, and patients' immunoreactivity to cross-reactive heat shock protein (HSP) epitope peptide from P. gingivalis HSP60. Methods: Twenty-five patients with moderate periodontitis had their gingival connective tissue harvested of gingival mononuclear cells during an open flap debridement procedure and peripheral blood was drawn by venipuncture to collect serum. The mean level of interproximal alveolar bone was calculated to be used as an index for periodontal disease severity for a given patient. Each of selected immunologic parameters was subject to statistical management to seek their correlations with the severity of periodontal disease. Results: A significant correlation could not be identified between serum IgG titers against specific bacteria (Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, and Streptococcus mutans) and the severity of periodontal disease. Expression of interleukin (IL)-10 by gingival mononuclear cells was statistically significant in the group of patients who had higher levels of alveolar bone height. However, a similar correlation could not be demonstrated in cases for IL-4 or interferon-$\gamma$. Patients' serum reactivity to cross-reactive epitope peptide showed a significant correlation with the amount of alveolar bone. Conclusions: It was concluded that expression of IL-10 by gingival mononuclear cells and patients' sero-reactivity to the cross-reactive HSP peptide of P. gingivalis HSP60 were significantly correlated with alveolar bone height.

The road not taken: beyond our embarrassment and skepticism

Choi, Jeom-Il Korean Academy of Periodontology 2019 Journal of Periodontal & Implant Science Vol.49 No.3

Vaccines against periodontitis: a forward-looking review

Choi, Jeom-Il,Seymour, Gregory J. Korean Academy of Periodontology 2010 Journal of Periodontal & Implant Science Vol.40 No.4

Periodontal disease, as a polymicrobial disease, is globally endemic as well as being a global epidemic. It is the leading cause for tooth loss in the adult population and has been positively related to life-threatening systemic diseases such as atherosclerosis and diabetes. As a result, it is clear that more sophisticated therapeutic modalities need to be developed, which may include vaccines. Up to now, however, no periodontal vaccine trial has been successful in satisfying all the requirements; to prevent the colonization of a multiple pathogenic biofilm in the subgingival area, to elicit a high level of effector molecules such as immunoglobulin sufficient to opsonize and phagocytose the invading organisms, to suppress the induced alveolar bone loss, or to stimulate helper T-cell polarization that exerts cytokine functions optimal for protection against bacteria and tissue destruction. This article reviews all the vaccine trials so as to construct a more sophisticated strategy which may be relevant in the future. As an innovative strategy to circumvent these barriers, vaccine trials to stimulate antigen-specific T-cells polarized toward helper T-cells with a regulatory phenotype (Tregs, $CD_{4+}$, $CD_{25+}$, $FoxP_{3+}$) have also been introduced. Targeting not only a single pathogen, but polymicrobial organisms, and targeting not only periodontal disease, but also periodontal disease-triggered systemic disease could be a feasible goal.

IgG subclass-dependent Recognition of Porphyromonas Gingivalis Antigens in the Early-onset Periodontitis

Choi, Jeom-Il,Yoshimobu, Fuminobu,Schifferle, Robert E.,Okuda, Katsuji The Korean Academy of Periodontoloy 1999 Journal of Periodontal & Implant Science Vol.29 No.4

본 연구는 세 종류의 Porphyromonas gingivalis(Pg) antigen의 IgG subclass associated recognition을 평가하기 위해 수행했었다. 총 35명의 조기발병형치주질환자중, Pg381에 대한 IgG2항체의 증가를 보이는 5명이 급속진 행형 치주질환자, IgG4의 증가를 보이는 6명의 환자(국소유년형 치주질환자 2명과 급속진행 형 치주질환자 4명), IgG2+4의 증가를 보이는 2명의 급속진행형 치주질환자 그리고 IgG1+2+4의 증가를 보이는 8명의 환자(국소 유년형 치주질환자 2명과 급속진행형 치주질환자 6명)으로 구성된 21명의 환자를 dot immunoblot analysis를 위해 선택했다. 실험에 사용된 정제된 항원은 Pg381에서 추출한 43-kd fimbrilin protein과 lipoplysaccharide(LPS), Pg A7A1-28(ATCC 53977)에서 추출한 capsularpolysaccharide(CPS)였다. Immunoblotting pattern은 IgG4 antibody가 fimbrial antigen에 강력히 반응함을 보여주었다. Fimbriae에 잘 반응하는 몇몇의 IgG4 antibody역시 antigen에 대해 양성반응을 보였다. 대조적으로 IgG2는 CPS antigen을 일차적으로 인식했다. 전부는 아니지만 대부분의 경우, single이나 group화된 IgG subclass는 모두 LPS antigen을 인식하지 못했다. 같은 group에서 염색강도의 개인적인 차이는 증명되었다. 이런 결과는 조기발 병형 치주질환에서 Pg의 fimbriae와 CPS가 immunodominant antigen이 될 수 있음을 제시한다. 더욱이 IgG subclass antibody가 이런 Pg의 immunoglobulin antigen을 선택적으로 인식함을 알았고, 이는 조기발병형치주질환의 병리에 immunodominant antigen과 함께 IgG의 기능적인 역할을 고려해야 함을 제시한다.

Immunoglobulin Allotypes and Immunoglobulin G Subclass Responses to Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in Early-Onset Periodontitis

CHOI, JEOM-IL,HA, MEE-HYE,KIM, JUN-HONG,KIM, SUNG-JO 부산대학교 유전공학연구소 1996 분자생물학 연구보 Vol.12 No.-

The present study was performed to estimate the observed frequencies of the immunoglobulin heavy-chain(Gm) and light-chain (Km) allotypes among patients with early-oneset periodontitis (EOP) and their effect on the IgG2 subclass responses against Actinobacillus actinomycetemcomitans Y4 and Porphyromaonas gingivalis 381, respectively. Sixty-nine EOP patients, including 11 with localized juvenile periodontitis (LJP), 19 who had LJP, 15 with LJP-rapidly progressing periodontitis (RPP), and 24 with RPP, were examined for the Gm and Km allotypes by a hemagglutination inhibition test. Levels of immunoglobulin G2 (IgG2) antibodies against the two organisms were determined by enzyme-linked immunosorbent assay. Fifty race-and age-matched, periodontally healthy subjects were also included as a control group. The observed frequencies of the Gm haplotype afnb and Km(1) were significantly higher in the RPP and LJP groups, respectively. The G2m(n) + group of those with RPP and the Km(1) + group of those with LJP had significantly higher levels of IgG2 antibodies to A. actinomycetemcomitans and P. gingivalis, respectively. The results indicate that linkage disequilibrium of the G2m(n) locus in RPP patients or the Km(1) locus in LJP patients may be associated with high IgG2 antibody responses to the respective bacteria. It was reasoned that the IgG2 antibody responses are associated with the immunoglobulin allotypes. The function of IgG2 antibodies in their reaction to different bacterial antigens may be interpreted as either protective or nonprotective in the two different types of EOP (i.e., LJP and RPP).

Identification of mono- or poly-specific monoclonal antibody to Porphyromonas gingivalis heat-shock protein 60

Choi, Jeom-Il,Lee, Sang-Yull,Kim, Koan-Hoi,Choi, Bong-Kyu,Kim, Myung-Jin Korean Academy of Periodontology 2011 Journal of Periodontal & Implant Science Vol.41 No.2

Purpose: The aim of this study was to define the immunoreactive specificity of Porphyromonas gingivalis (P. gingivalis) heat shock protein (HSP) 60 in periodontitis and atherosclerosis. Methods: In an attempt to define the cross-reactive bacterial heat-shock protein with human self-antigen at molecular level, we have introduced a novel strategy for cloning hybridoma producing anti-P. gingivalis HSP 60 which is polyreactive to bacterial HSPs or to the human homolog. Results: Five cross-reactive clones were obtained which recognized the #19 peptide (TLVVNRLRGSLKICAVKAPG) among 37 synthetic peptides (20-mer, 5 amino acids overlapping) spanning the whole molecule of P. gingivalis HSP 60. We have also established three anti-P. gingivalis HSP 60 monoclonal antibodies demonstrating mono-specificity. These clones recognized the #29 peptide (TVPGGGTTYIRAIAALEGLK). Conclusions: Peptide #19 and #29 of P. gingivalis HSP 60 might be important immunoreactive epitopes in the immuno-pathogenic mechanism of bacterial antigen-triggered autoimmune diseases.

Symposium : New Trends in Periodontal Therapy: Optimizing Papillary Dimension for Esthetic Restoration-Multidisciplinary Perio-ortho-prosthodontic Approaches

( Jeom Il Choi ) 대한치주과학회 2010 대한치주과학회 학술대회자료집 Vol.2010 No.1

Prior Immunization with Fusobacterium Nucleatum Interferes with Opsonophagocytosis Function of Sera against Porphyromonas Gingivalis

Choi, Jeom-Il,Borrello, Melinda A.,Cutler, Christopher W.,Zauderer, Maurice The Korean Academy of Periodontoloy 2000 Journal of Periodontal & Implant Science Vol.30 No.1

본 연구는 Balb/c mice를 이용하여 Porphyromonas gingivalis 381(Pg)로 면역하기 전에 Fusobacterium nucleatum ATCC 10953(Fn)로 면역한 Group 1(N=10)과 Pg 로만 단독 면역을 시행한 Group 2(N=10)로부터 채취한 혈청의 Pg에 대한 식균능력을 비교하는 데 그 목적이 있다. 면역 후 혈청항체는 Pg에 대해 현저히 상승하였으나, 두 그룹간의 평균 항체역가는 통계적으로 차이가 없었다. 식균능력을 비교한 결과, Pg로만 단독 면역한 경우 식균능력이 Fn으로 먼저 면역한 Group 1의 경우보다 현저히 높았으며, 혈청항체역가와 식균지수와는 긴밀한 상관관계를 보였다. 결론적으로 치주세균의 사전 감염은 후속적인 세균감염에 대한 숙주 면역기능(식균능력)에 교란을 가져 올 수 있다.

과대 동요치아를 이용한 계속금관 가공의치의 임상적 연구

최점일(Jeom Il Choi),이재봉(Jae Bong Lee) 대한치과의사협회 1985 대한치과의사협회지 Vol.23 No.12