Co/Mn/Br계 촉매상에서 p-자일렌 액상산화반응의 공정변수에 관한 연구

손용배,박경린,류태공,김정희,오인석,김영호,양현수 忠南大學校 産業技術硏究所 2001 산업기술연구논문집 Vol.16 No.2

Liquid phase oxidation of p-xylene using molecular oxygen has been carried out on the Co/Mn/Br-based catalyst system in acetic acid as a solvent. Process parameters(total pressure, partial pressure of oxygen and temperature) and kinetics of the reaction were studied as a basic study in the viewpoint for the development of new catalyst system and process. The total pressure above 20atm and the partial pressure of oxygen above 5atm were required to keep the reactant in the liquid phase and to overcome the mass transfer limitation of oxygen. The oxidation rate conversion were increased with increasing reaction temperature to the 100∼190℃ range. For a given initial concentration of p-xylene, the oxidation rate and the apparent activation energy were found to be of first-order and 4.24 ㎉/㏖, respectively. The effect of the third components added to the Co/Mn/Br catalyst was also studied. It was found that Ni is only a good additive that can promote the catalyst performance.

A case of angiolymphoid hyperplasia with eosinophilia treated with cryotherapy

( Seok Hyun Han ),( Jun Young Seong ),( Sook Hyun Kong ),( Ji Hyun Kim ),( Ho Seok Seo ),( Yu Sung Choi ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1

Angiolymphoid hyperplasia with eosinophilia (ALHE) is an uncommon benign vasoproliferative disorder of unclear origin that usually affects middle-aged women. ALHE typically presents with solitary or multiple pink to red-brown colored dome-shaped papules or nodules occurring most frequently on the head and neck. Histopathologically, there are proliferation of large endothelial cells lining vascular spaces, and lymphocytic and eosinophilic inflammatory cell infiltrations in the dermis. The treatment of choice of ALHE is surgical excision, but due to the high rate of recurrence, other less invasive therapeutic modalities such as cryotherapy can be employed. A 74-year-old man presented with 20-year history of multiple various-sized red-brown colored nodules on the left anterior chest. Histopathologic findings from nodule showed vascular proliferation and dilation with lymphocyte and eosinophil infiltration in the dermis. The patient was diagnosed with ALHE and treated with cryotherapy 4 sessions at 3-week intervals. The lesions showed good response. Herein we report a case of ALHE treated with cryotherapy.

Effects of anti-mullerian hormone supplementation on cryopreserved and transplanted ovarian tissues

Hyun Sun Kong,Jaewang Lee,Eun Jung Kim,Hye Won Youm,Jung Ryeol Lee,Byung Chul Jee,Chang Suk Suh,Seok Hyun Kim 한국발생생물학회 2014 한국발생생물학회 학술발표대회 Vol.2014 No.9

Ovarian tissue cryopreservation and transplantation causes follicle depletion. To overcome this problem, we investigate the effect of Anti-Müllerian hormone (AMH), a follicle recruitment control hormone, supplementation before and/or after mouse ovarian transplantation. A total of 120 5-week-aged BD F-1 female mice were used. The mice were randomly divided into four groups according to AMH doses (0, 5, 25, 125 μg/mL, respectively). AMH was injected intraperitoneally on every other day for a week before, after, or before and after transplantation of ovaries under kidney capsules was performed. One week after transplantation, follicular normality was evaluated by histological analysis and TUNEL assay. In Group A and C, morphologically intact follicle (G1) ratios of AMH treated groups showed no statistically significant difference. In Group B, G1 ratios of 25 and 125 μg/mL of AMH treated groups were higher than those of 5 μg/mL treated group, but there was no improvement in G1 ratio after AMH treatment. In every group, apoptotic follicle ratios did not show any trend according to AMH treatment. Proportions of primordial follicle were not significantly different according to AMH treatment in all groups. The result of the present study demonstrated that AMH treatment during on transplantation of cryopreserved ovaries has no significant effect on follicle survival and prevention of follicle depletion.

A case of erythematotelangiectatic rosacea successfully treated with carvedilol

( Seok Hyun Han ),( Seong Jun Young ),( Sook Hyun Kong ),( Ji Hyun Kim ),( Ho Seok Suh ),( Yu Sung Choi ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1

According to the National Society of Rosacea, rosacea is classified as erythematotelangiectatic rosacea (ETR), papulopustular rosacea, phymatous rosacea and ocular rosacea. The ETR subtype is typified by frequent episodes of facial flushing, telangiectasias, and persistent centrofacial erythema. Severe flushing can cause significant physical discomfort and emotional stress to the patients, and currently no satisfactory treatments are available. Nonselective ß-blockers decrease sympathetic activity and also have been reported to suppress flushing. The mechanism of ß-blockers in treating ETR may be by way of blocking the ß2-adrenergic receptor on the smooth muscle of cutaneous arterial blood vessels, resulting in vasoconstriction. Especially carvedilol, a nonselective ß-adrenergic blocking agent, demonstrated more potent antioxidant properties compared with other ß blockers and may offer an added advantage over the traditional ß blockers. A 35-year-old man presented with facial flushing with persistent erythema, and telangiectasia accompanied by burning and itchy sensation that provoked by various stimuli, including seasonal variation and stress. The patient was diagnosed with ETR and treated with carvedilol 6.25mg daily. The symptoms of ETR were improved with minimal facial erythema and flushing without a hot or burning sensation. Herein we report a case of ETR successfully treated with carvedilol.

Effect of Exogenous Anti-Mullerian Hormone Treatment on Cryopreserved and Transplanted Mouse Ovaries

Kong, Hyun Sun,Kim, Seul Ki,Lee, Jaewang,Youm, Hye Won,Lee, Jung Ryeol,Suh, Chang Suk,Kim, Seok Hyun SAGE Publications 2016 Reproductive sciences Vol.23 No.1

<P>Follicle loss occurs after ovary cryopreservation and transplantation. To preserve the follicle pool of cryopreserved or grafted ovaries, anti-Mullerian hormone (AMH), which inhibits ovarian follicle recruitment, was used in a mouse model. In experiment 1, ovaries were vitrified warmed with different doses of AMH (0, 5, 15, or 45 g/mL) supplementation. In experiment 2, AMH (0, 50, 250, and 1250 g/mL) was injected into mice before and/or after cryopreserved ovary autotransplantation, and the recipients remained for 7 or 28 days after grafting. Ovaries were evaluated by follicle morphology, density, and apoptosis ratio. Additionally, serum follicle-stimulating hormone was measured in experiment 2. Significantly decreased follicle apoptosis were detected in AMH-treated groups when compared to the control ovaries in experiment 1, meanwhile no positive effect of exogenous AMH was found in experiment 2. Thus, we suggest AMH supplementation during ovary vitrification warming has beneficial effect on reducing follicle apoptosis.</P>

Prognostic Value of Glioma Cancer Stem Cell Isolation in Survival of Primary Glioblastoma Patients

Kong, Byung Ho,Moon, Ju Hyung,Huh, Yong-Min,Shim, Jin-Kyoung,Lee, Ji-Hyun,Kim, Eui-Hyun,Chang, Jong Hee,Kim, Dong-Seok,Hong, Yong-Kil,Kim, Sun Ho,Lee, Su-Jae,Kang, Seok-Gu Hindawi Publishing Corporation 2014 Stem cells international Vol.2014 No.-

<P>Cancer stem cells (CSCs) have been reported to be critical in the initiation, maintenance, and progression of cancers. The expression of stem cell markers, such as podoplanin (PDPN), CD133, and nestin, may have been correlated with malignant progression. However, the effects of CSCs and stem cell markers on clinical outcomes in cancer patients remain unclear. In this study, we assessed the prognostic roles of glioma CSCs (gCSCs) isolation and stem cell markers in patients with primary glioblastoma (pGBM). A cohort of 39 patients with pGBM was separated into two groups, those positive or negative for gCSCs, and the correlation between gCSC and patient survival was evaluated. We observed significantly different cumulative survival (<I>P</I> = 0.045) when comparing patients positive for gCSCs patients and negative for gCSC. Among the patients positive for gCSCs, we observed no significant differences in survival between those whose gCSCs were each positive or negative for PDPN, CD133, or nestin. This study strongly supports the prognostic value of gCSCs isolation on the survival of patients with pGBM.</P>

Increased in vivo angiogenic effect of glioma stromal mesenchymal stem-like cells on glioma cancer stem cells from patients with glioblastoma.

Kong, Byung Ho,Shin, Hyun-Do,Kim, Se-Hoon,Mok, Hyun-Su,Shim, Jin-Kyoung,Lee, Ji-Hyun,Shin, Hye-Jin,Huh, Yong-Min,Kim, Eui-Hyun,Park, Eun-Kyung,Chang, Jong Hee,Kim, Dong-Seok,Hong, Yong-Kil,Kim, Sun Ho Lychnia 2013 International journal of oncology Vol.42 No.5

<P>The presence of glioma stromal mesenchymal stem?like cells (GS-MSLCs) in tumors from glioma patients has been previously reported. The mechanisms through which these cells function as a part of the glioma microenvironment, however, remain incompletely understood. We investigated the biological effects of GS-MSLCs on glioma cancer stem cells (gCSCs), testing the hypothesis that GS-MSLCs alter the biological characteristics of gCSCs. GS-MSLCs and gCSCs were isolated from different glioblastoma (GBM) specimens obtained from patients. In in vitro experiments, gCSCs were cultured alone or co-cultured with GS-MSLCs, and gCSCs cell counts were compared between the two groups. In addition, two groups of orthotopic GBM xenografts in mice were created, one using gCSCs from the monoculture group and one using gCSCs isolated from the co-culture group, and tumor volume and survival were analyzed. Furthermore, in vivo proliferation, apoptosis and vessel formation were examined using immunohistochemical analyses. In vitro cell counts for gCSCs co-cultured with GS-MSLCs increased 3-fold compared to gCSCs cultured alone. In orthotopic xenograft experiments, mice injected with gCSCs isolated from the co-culture group had significantly larger tumor volume, measured on day 40 after injection, and their survival times were shorter. Immunohistochemical analysis showed increased tumor expression of CD31, indicative of enhanced microvessel formation in mice injected with gCSCs co-cultured with GS-MSLCs compared to mice injected with gCSCs cultured alone. However, proliferation (PCNA) and apoptosis (TUNEL) markers showed no significant difference between the two groups. In conclusion, GS-MSLCs may influence the biological properties of gCSCs, shifting them towards a more aggressive status; moreover, increased angiogenesis may be a critical component of this mechanism.</P>

Primary cutaneous coinfection with Mycobacterium tuberculosis and Cryptococcus laurentii

( Seok Hyun Han ),( Seong Jun Young ),( Sook Hyun Kong ),( Ji Hyun Kim ),( Ho Seok Suh ),( Yu Sung Choi ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1

Cutaneous infection can be classified into localized infection after traumatic inoculation and skin manifestation due to a hematogenous dissemination. Primary cutaneous tuberculosis is rare, usually manifests as erythematous brownish papule or nodule that might later break down to form an ulcer with undermined edges. Cryptococcus laurentii is an extremely rare human pathogen that was previously considered saprophytic and non-pathogenic to humans, but it has been isolated as the etiologic agent of infections such as skin infection, endophthalmitis, lung abscess and fungemia. A 58-yearwoman presented with 3-month history of tender two coin-sized erythematous to brownish nodules on the both forearm. Histopathologic findings from nodules showed ulcer with diffuse dermal inflammatory cell infiltration, and focal aggregation of numerous acid-fast bacilli which was identified by AFB stain. Polymerase chain reaction for Mycobacterium tuberculosis was positive. The patient was diagnosed with primary cutaneous tuberculosis and treated with anti-tuberculosis medication. Primary skin lesion on the right forearm healed up with scarring, but lesion on the left forearm was resistant to anti-tuberculosis medication. Additional fungal culture from lesion on the left forearm showed positive result for Cryptococcus laurentii. Herein we report primary cutaneous coinfection with mycobacterium tuberculosis and cryptococcus laurentii.

A case of epidermal cyst mixed with organoid nevus

( Ji Hyun Kim ),( Seong Jun Young ),( Sook Hyun Kong ),( Seok Hyun Han ),( Ho Seok Suh ),( Yu Sung Choi ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1

Epidermal cyst is the most common subcutaneous tumor that usually involves the scalp, face, or trunk. It is a slowly growing, elevated, round, and firm intradermal or subcutaneous tumor that grows 1.5 cm in diameter. Histopathologically, it is lined by stratified epithelium, containing central, eosinophilic, keratinaceous debris. Organoid nevus, also known as nevus sebaceous of Jadassohn, is a kind of congenital hamartoma. It presents as a waxy, hairless, verrucous plaque located on the scalp, face, or neck. Histologically, epidermal hyperplasia and increased numbers of sebaceous glands and apocrine glands in the dermis are observed. The combination of organoid nevus with other tumors of epidermal or adnexal origin has often been described in literatures. A 6-year old boy presented with 2-month history of tender 1.7 cm x 1.7 cm sized erythematous crusted surfaced subcutaneous nodule on the scalp. The lesion was elliptically excised and histopathologic findings from the excised specimen showed keratin-filled epithelial-lined cyst with epidermal hyperplasia and increased numbers of sebaceous glands and apocrine glands in the dermis. Therefore, the patient was diagnosed with epidermal cyst mixed with organoid nevus. Herein we report a case of epidermal cyst mixed with organoid nevus.

[P094] A case of subcutaneous eccrine hidrocystoma located in the muscular layer

( Ji Hyun Kim ),( Sook Hyun Kong ),( Seok Hyun Han ),( Yong Woo Oh ),( Ho Seok Suh ),( Yu Sung Choi ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.1

Eccrine hidrocystoma is a benign cystic neoplasm derived from dilatation of the excretory sweat ducts. Its pathogenesis is explained as a retention cyst due to obstruction of the eccrine duct. Clinically it is translucent, skin-colored papules predominantly located on the periorbital or malar area of the face and neck. Histopathologically, eccrine hidrocystoma shows one or more unilocular cysts in the dermis, and cystic wall consists of flattened epithelial cells partially lined by two layers of cuboidal epithelium. In case of single lesion, hidrocystomas may be removed by simple excision, electrodesiccation or CO₂ laser. A 52-year-old female presented with a 0.2 x 0.2 cm sized asymptomatic firm subcutaneous nodule on the right lower orbital area. The patient have done excisional biopsy and diagnosed with foreign body reaction, but the lesion was recurred. We decided to operate the lesion with excision and V-Y advancement flap. Operational findings showed multiple various-sized cysts located from dermis to the muscle layer. The histopathologic specimen revealed dilated cystic structure with one or two layers of cuboidal or flattened epithelial cells. The patient was diagnosed with eccrine hidrocystoma. Herein, we present a case of subcutaneous eccrine hidrocystoma located in the muscular layer.