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Hanyu Zhu,Yuan Yuan,Juan Liu,Liesheng Zheng,Liguo Chen,Aimin Ma 한국미생물학회 2016 The journal of microbiology Vol.54 No.5
To gain insights into dimorphism, cell wall polysaccharides from Tremella fuciformis strains were obtained from alkaliextracted water-soluble fractions PTF-M38 (from the mycelial form), PTF-Y3 and PTF-Y8 (from the yeast form) of T. fuciformis strains were used to gain some insights into dimorphism study. Their chemical properties and structural features were investigated using gel permeation chromatography, gas chromatography, UV and IR spectrophotometry and Congo red binding reactions. The results indicated that the backbones of PTF-M38, PTF-Y3 and PTF-Y8 were configured with α-linkages with average molecular weights of 1.24, 1.08, and 1.19 kDa, respectively. PTF-M38 was mainly composed of xylose, mannose, glucose, and galactose in a ratio of 1:1.47:0.48:0.34, while PTF-Y3 and PTF-Y8 were mainly composed of xylose, mannose and glucose in a ratio of 1:1.65:4.06 and 1:1.21:0.44, respectively. The sugar profiles of PTF-M38, PTF-Y3 and PTF-Y8 were also established for further comparison. These profiles showed that all three polysaccharides contained the same sugars but in different ratios, and the carbon sources (xylose, mannose, glucose, and galactose) affected the sugar ratios within the polysaccharides.
( Hanyu Zhu ),( Xueyan Sun ),( Dongmei Liu ),( Liesheng Zheng ),( Liguo Chen ),( Aimin Ma ) 한국균학회 2017 Mycobiology Vol.45 No.4
An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus―Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32. The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9. The yields of RNA were between 108 and 213 μg/g fresh wt. Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed. This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides.
Alariqi Muna,Wei Hao,Cheng Junqi,Sun Yiwen,Zhu Hanyue,Wen Tianwang,Li Yapei,Wu Chenglin,Jin Shuangxia,Cao Jinglin 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.6
Tobacco bacterial wilt caused by Ralstonia solanacearum invades tobacco plants during the whole growth period afecting yield and quality. However, the transcriptome profling of tobacco plant in response to bacterial wilt has not been well studied. In this study, we identifed the transcriptional profles of bacterial wilt-resistant (ac Yanyan97) and -susceptible (ac Honghuadajinyuan) tobacco cultivars infected with R. solanacearum at six time points by RNA sequencing. Gene expression analysis showed that the resistant cultivar manifested a faster change in the expression of defense-related genes than the susceptible cultivar during R. solanacearum infection, by which more diferentially expressed genes (DEGs) were up-regulated rather than down-regulated at all time points. Functional analysis indicated that DEGs were involved in plant hormones, glutathione and secondary metabolic pathways associated with tobacco resistance to bacterial wilt induced by R. solanacearum. Through subsequent Short Time-series Expression Miner (STEM) and weighted correlation network (WGCNA) analyses, the phenylpropanoid metabolic pathway was identifed as a key pathway for tobacco defense against R. solanacearum infestation. In summary, our results provide transcriptomic profles of tobacco response to R. solanacearum infestation