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Coronatine Induces Stomatal Reopening by Inhibiting Hormone Signaling Pathways
Uddin Shahab,Bae Dongryeoul,차준영,안경익,김외연,김민갑 한국식물학회 2022 Journal of Plant Biology Vol.65 No.5
Coronatine (COR), a phytotoxin, aids Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) invasion by suppressing stomatal immunity, thus promoting bacterial replication. COR also promotes disease development, increases disease resistance in non-infected plant parts, suppresses cell wall defenses, and delays the hypersensitive response. Moreover, COR inhibits stomatal closure by influencing reactive oxygen species (ROS) synthesis, by NADPH oxidases RBOHD/F, which highlights the role of NADPH oxidases in guard cell signaling. Plant resistance to Pst DC3000 is dependent on guard cell-specific mitogen-activated protein kinases (MAPKs) that function downstream of ROS in salicylic acid (SA) and abscisic acid (ABA)-mediated stomatal closure. COR inhibits the ABA signal transduction pathway in guard cells, which is linked to pathogen-associated molecular pattern (PAMP)-induced stomatal closure. Furthermore, COR uses the plant jasmonate (JA) receptor coronatine-insensitive 1 (COI1) to activate multiple NAC transcription factors, which then regulate SA metabolic genes, preventing the accumulation of SA. In this review, we highlight the negative impact of COR on ROS production, SA and ABA signaling transduction, and consequently stomatal immunity. We believe that the inhibitory effects of COR on SA and ABA-induced stomatal closure require substantially more research to fully understand the stress adaptation mechanisms of plants.
Jinhyun Kim,Jongchul Yu,Dongryeoul Bae,Kun-Ho Seo 한국예방수의학회 2019 예방수의학회지 Vol.43 No.4
The poultry red mite (PRM), Dermanyssus gallinae, is one of the most important external parasites in the poultry industry. PRM adheres to birds’ bodies, and its hematophagy causes anemia and itching, and reducing egg production (~20%). Fipronil is a toxic pesticide used to control PRM. Thus, an environmentally friendly alternative control agent is needed. In this study, the effectiveness of various environmentally friendly PRM control measures including an acaricide consisting of synthetic silica, clove extracts, garlic extracts, and a probiotic solution were evaluated, comparing them to a combined treatment using the chemical acaricides. Each agent was tested in vitro using two approaches, in which PRMs were either added to environments containing acaricide, or sprayed in situ. Within 10 hours of inoculation, all the environmentally friendly acaricides except for the probiotic solution killed or rendered immotile significantly more mites than the control, normal saline (p < 0.05). The performance of the environmentally friendly acaricides, except for synthetic silica, was significantly weaker than that of commercial chemical acaricides. (p < 0.05). Further tests to determine the optimal concentration of amorphous synthetic silica agent for complete PRM control found this to be 400 g/L. PRM motility following treatment showed the same pattern: the synthetic silica agent and chemical acaricide killed mites, while application of other environmentally friendly agents resulted in living, but immotile, insects, or those with reduced motility. As a result, these environmentally friendly acaricides, especially the synthetic silica agent, could be used as alternatives to chemical acaricides for PRM control.
Jung-Whan Chon,서건호,Dongryeoul Bae,Ji-Hee Park,Saeed Khan,성기돈 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.3
Clostridium perfringens causes diarrhea and other diseases in animals and humans. We investigated the prevalence, toxin gene profiles, and antibiotic resistance of C. perfringens isolated from diarrheic dogs (DD) and non-diarrheic dogs (ND) in two animal hospitals in Seoul, Korea. Fecal samples were collected from clinically DD (n = 49) and ND (n = 34). C. perfringens was isolated from 31 of 49 DD (63.3%) and 21 of 34 ND dogs (61.8%). All C. perfringens strains were positive for the a toxin gene, but not for the b, e, or i toxin genes; therefore, all strains were identified as type A C. perfringens. All isolates were cpe-negative, whereas the b2 toxin gene was identified in 83.9% and 61.9% of isolates from DD and ND, respectively. Most isolates were susceptible to ampicillin (94%), chloramphenicol (92%), metronidazole (100%), moxifloxacin (96%), and imipenem (100%). However, 25.0% and 21.2% of isolates were resistant to tetracycline and clindamycin, respectively. Molecular subtyping of the isolated strains was performed by using pulsed-field gel electrophoresis. Fifty-two isolates were classified into 48 pulsotypes based on more than 90% similarity of banding patterns. No notable differences were observed among the isolates from DD and ND.
( Jung-whan Chon ),( Kun-ho Seo ),( Dongryeoul Bae ),( Binn Kim ),( Dongkwang Jeong ),( Kwang-young Song ) 한국낙농식품응용생물학회(구 한국유가공학회) 2020 Journal of Dairy Science and Biotechnology (JMSB) Vol.38 No.4
This study was conducted to evaluate the antibacterial activity against Cronobacter sakazakii and Salmonella enteritidis as well as the sensory attributes of milk products supplemented with various concentrations (control, 0.5%, 1.0%, 1.5%, and 2.0%) of clove oil. In this study, clove oil was shown to have strong antibacterial activities. In addition, all the samples were assessed by ten researchers trained in five sensory attributes, namely, taste, flavor, color, texture, and overall acceptability. Compared to the control, 5% clove oil supplemented was the best in market milk, while in yogurt and kefir, 1.0% supplementation was the best. In terms of sensory attributes, the low score of color and flavor of market milk, yogurt, and kefir is attributed to the characteristics of the supplemented clove oil. Consequently, this study presents the possibility of producing bio-functional milk products supplemented with clove oil, and for controlling the growth of foodborne pathogenic bacteria in milk products using clove oil.
( Jung-whan Chon ),( Kun-ho Seo ),( Jin-hyeok Yim ),( Dongryeoul Bae ),( Binn Kim ),( Tae-jin Kim ),( Dongkwan Jeong ),( Kwang-young Song ) 한국낙농식품응용생물학회(구 한국유가공학회) 2021 Journal of Dairy Science and Biotechnology (JMSB) Vol.39 No.1
Enterobacter sakazakii (Cronobacter spp.) causes meningitis, necrotizing enterocolitis, sepsis, and bacteremia in neonates and children and has a high mortality rate. For rapid E. sakazakii detection, various differential and selective media containing α-glucosidase substrates, such as 5-bromo-4-chloro-3-indolyl-α-D-glucopyranoside (BCIG) or 4-methylumbelliferyl- α-D-glucoside (α-MUG), have been developed as only E. sakazakii exhibits α -glucosidase activity in the genus Enterobacter. However, Escherichia vulneris (family: Enterobacteriaceae) can also utilize α-glucosidase substrates, thereby resulting in false positives. Various iron sources are known to promote the growth of gram-negative bacteria. This study aimed to develop a selective medium containing α-glucosidase substrates for E. sakazakii detection that would eliminate false positives, such as those of E. vulneris, and to determine the role of iron source in the medium. Three previously developed (TPD) media, i.e., Oxoid, OK, and VRBG, and the medium developed in this study, i.e., NGTE, were evaluated using 58 E. sakazakii and 5 non-E. sakazakii strains. Fifty-four E. sakazakii strains appeared as fluorescent or chromogenic colonies on all four media that were assessed. Two strains showed colonies on NGTE medium and not on TPD media. In contrast, the remaining two strains showed colonies on TPD media and not on NGTE medium. None of the non-E. sakazakii strains showed fluorescent or chromogenic colonies on any of the evaluated media except E. vulneris, which showed colonies on TPD media and not on NGTE medium. This study demonstrated that the newly developed NGTE medium was not only equally efficient in promoting the growth of bacterial colonies when compared with the currently available media but also eliminated false positives, such as E. vulneris.
( Ji-yeon Hyeon ),( Kun-ho Seo ),( Jung-whan Chon ),( Dongryeoul Bae ),( Dongkwang Jeong ),( Kwang-young Song ) 한국낙농식품응용생물학회(구 한국유가공학회) 2020 Journal of Dairy Science and Biotechnology (JMSB) Vol.38 No.4
Salmonella spp. is the most common cause of gastrointestinal food poisoning worldwide, and human salmonellosis is mostly caused by the consumption of contaminated food. Therefore, the development of rapid detection methods for Salmoenlla spp. and rapid identification of the source of infection by subtyping are important for the surveillance and monitoring of food-borne salmonellosis. Therefore, this review introduces (1) History and nomenclature of Salmoenlla spp., (2) Epidemiology of Salmoenlla spp., (3) Detection methods for Salmoenlla spp. - conventional culture method, genetic detection method, molecular detection methods, and aptamer, and (4) Subtyping methods for Salmoenlla spp. - pulsed-field gel electrophoresis and repetitive sequence-based polymerase chain reaction (PCR).