http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : ( Chayul Lee ) (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
Effects of Myxococcus fulvus KYC4048 Metabolites on Breast Cancer Cell Death
( Chayul Lee ),( Sanghyun Park ),( Ikhbayar Ayush ),( Kyungyun Cho ),( Sung Soo Kim ),( Insug Kang ),( Wonchae Choe ),( Yoon-seong Kim ),( Kyung-sik Yoon ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.5
Using MCF7 breast cancer cells, we tested the anticancer activity of metabolites from 130 strains of myxobacteria newly isolated in South Korea. Of these, three strains whose metabolites had high anticancer activity and low cell toxicity were selected and identified by their fruiting body morphology, cell morphology, and 16S rRNA sequence. Strains KYC4030 and KYC4048 were determined to be Myxococcus fulvus, whereas strain KYC4081 was identified as Corallococcus coralloides. We found that metabolites of M. fulvus KYC4048 demonstrated no toxicity in normal cells but specifically induced cancer cell death by suppressing the expression of WNT2B. This discovery highlights the value of assessing the metabolic and biomedical potential of myxobacteria, even those that are already known but were isolated from new areas, and the possible use of metabolites from M. fulvus KYC4048 in cancer treatment.
-
Chondromyces crocatus 자실체 형성의 정량적 유도 방법 개발
이차율,신혜진,조경연,Lee, Chayul,Shin, Hyejin,Cho, Kungyun 한국미생물학회 2014 미생물학회지 Vol.50 No.3
Chondromyces crocatus의 자실체 형성에 대한 정량적 연구를 위하여 필수적인 분산된 세포현탁액 제조방법을 개발하였다. C. crocatus 세포들은 액체에서 서로 응집하는 특성이 있어 정량적 연구가 어렵다. 하지만 3% 한천을 함유한 casitone-yeast extract 평판배지에서 배양한 세포들은 물에 잘 분산되어 분산된 세포현탁액을 제조할 수 있었다. 이렇게 제조한 $2{\times}10^8cells/ml$ 농도의 세포현탁액 $20{\mu}l$를 영양분이 고갈된 평판배지 위에 올려놓고 배양하였을 때 C. crocatus 특유의 자실체를 형성하였다. Casitone과 같은 영양분을 첨가하였을 때는 자실체 형성이 저해되거나 변형되었으며, 한천의 농도가 높을수록 자실체의 가지수가 증가되었다. 조사된 최적조건에서 C. crocatus KYC2823은 24시간 내로 자실체 구조를 완성하였다. We have developed a method for the preparation of dispersed cell suspensions of Chondromyces crocatus, which is essential for quantitative studies of fruiting body formation. Cells of C. crocatus have a tendency to aggregate in liquid, hindering quantitative studies. However, cells grown on casitone-yeast extract agar plates, containing 3% agar, allowed the preparation of well-dispersed cell suspensions. Cell suspensions at a concentration of $2{\times}10^8cells/ml$, obtained by using this method, developed typical C. crocatus fruiting bodies when placed as $20{\mu}l$ spots on agar plates with no nutrient supplementation. The addition of nutrients such as casitone altered or inhibited fruiting body formation. Fruiting body branch formation increased with increasing agar content. Under optimum conditions, the formation of fruiting body structure in C. crocatus KYC2823 was completed within 24 h.
-
( Juha Park ),( Hee-jin Yoo ),( Ah-ran Yu ),( Hye Ok Kim ),( Sang Cheol Park ),( Young Pyo Jang ),( Chayul Lee ),( Wonchae Choe ),( Sung Soo Kim ),( Insug Kang ),( Kyung-sik Yoon ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.4
The Wnt/β-catenin signaling pathway is involved in breast cancer and Myxococcus fulvus KYC4048 is a myxobacterial strain that can produce a variety of bioactive secondary metabolites. Although a previous study revealed that KYC4048 metabolites exhibit anti-proliferative effects on breast cancer, the biochemical mechanism involved in their effects remains unclear. In the present study, KYC4048 metabolites were separated into polar and non-polar (ethyl acetate and n-hexane) fractions via liquid-liquid extraction. The effects of these polar and non-polar KYC4048 metabolites on the viability of breast cancer cells were then determined by MTT assay. Expression levels of Wnt/ β-catenin pathway proteins were determined by Western blot analysis. Cell cycle and apoptosis were measured via fluorescence-activated cell sorting (FACS). The results revealed that non-polar KYC4048 metabolites induced cell death of breast cancer cells and decreased expression levels of WNT2B, β-catenin, and Wnt target genes (c-Myc and cyclin D1). Moreover, the n-hexane fraction of non-polar KYC4048 metabolites was found most effective in inducing apoptosis, necrosis, and cell cycle arrest, leading us to conclude that it can induce apoptosis of breast cancer cells through the Wnt/β-catenin pathway. These findings provide evidence that the n-hexane fraction of non-polar KYC4048 metabolites can be developed as a potential therapeutic agent for breast cancer via inhibition of the Wnt/β-catenin pathway.
ScienceON
KISS연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
