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First Report of Lasiodiplodia theobromae Causing Gummosis on Citrus grandis (L.) Osbeck in Vietnam
Vo Thi Ngoc Trai,Tran Thi Thu Ha,Nguyen Bao Hung The Korean Society of Plant Pathology 2024 식물병연구 Vol.30 No.1
This study aims to isolate and identify the fungal pathogen responsible for gummosis disease affecting Thanh Tra pomelo in Vietnam. Through molecular identification utilizing primer pairs ITS5 and ITS4, the analysis pinpointed Lasiodiplodia theobromae as the specific fungal pathogen. Notably, the fungal colonies exhibited vigorous growth on potato dextrose agar. Initially, these colonies appeared whitish-grey, transforming into a black-grey hue within 5-7 days at a temperature of 30℃. According to previous reports, Phytophthora spp. was the most common pathogenic genus causing gummosis on Thanh Tra pomelo in Vietnam. To our knowledge, this is the first report on L. theobromae causing gummosis on Thanh Tra pomelo in Vietnam.
Nguyen Phuong Thuy(Nguyen Phuong Thuy ),Nguyen Ngoc Trai(Nguyen Ngoc Trai ),Bui Dang Khoa(Bui Dang Khoa ),Nguyen Hoang Xuan Thao(Nguyen Hoang Xuan Thao ),Vuong Tuan Phong(Vuong Tuan Phong ),Quach Van 한국육종학회 2023 Plant Breeding and Biotechnology Vol.11 No.2
Genetic variability and correlation analysis are fundamental references for the innovative development of breeding programs to improve varieties and desirable traits. In the present study, the correlation and path analysis was conducted to understand the association among yield, micronutrients (iron and zinc), and protein content under aerobic conditions in local rice landraces from various agro ecological regions of Karnataka, India. The grain yield per plant showed a significant positive correlation with plant height, the tiller number, spikelet fertility, flag leaf length, and test weight. The zinc content was negatively correlated with grain yield per plant. The phenotypic path-coefficient analysis revealed that the total number of tillers, grain length, test weight, and harvest index exhibited a positive direct effect on grain yield per plant, while Grain protein content showed a low direct effect on this trait. This study also indicated that harvest index, flag leaf length, spikelet fertility, and test weight could be considered to make for a higher yield.
Yoo, Hee-Bong,Park, Sang-Ryoul,Dong, Lianhua,Wang, Jing,Sui, Zhiwei,Pavš,ič,, Jernej,Milavec, Mojca,Akgoz, Muslum,Mozioğ,lu, Erkan,Corbisier, Philippe,Janka, Má,trai,Cosme, Bru American Chemical Society 2016 ANALYTICAL CHEMISTRY - Vol.88 No.24
<P>Enumeration-based determination of DNA copy-concentration was assessed through an international comparison among national metrology institutes (NMIs) and designated institutes (DIs). Enumeration-based quantification does not require a calibration standard thereby providing a route to 'absolute quantification', which offers the potential for reliable value assignments of DNA reference materials, and International System of Units (SI) traceability to copy number 1 through accurate counting. In this study, 2 enumeration based methods, flow cytometric (FCM) counting and the digital polymerase chain reaction (dPCR), were compared to quantify a solution of the pBR322 plasmid at a concentration of several thousand copies per microliter. In addition, 2 orthogonal chemical-analysis methods based on nucleotide quantification, isotope-dilution mass spectrometry (IDMS) and capillary electrophoresis (CE) were applied to quantify a more concentrated solution of the plasmid. Although 9 dPCR results from 8 laboratories showed some dispersion (relative standard deviation [RSD] = 11.8%), their means were closely aligned with those of the FCM-based counting method and the orthogonal chemical-analysis methods, corrected for gravimetric dilution factors. Using the means of dPCR results, the RSD of all 4 methods was 1.8%, which strongly supported the validity of the recent enumeration approaches. Despite a good overall agreement, the individual dPCR results were not sufficiently covered by the reported measurement uncertainties. These findings suggest that some laboratories may not have considered all factors contributing to the measurement uncertainty of dPCR, and further investigation of this possibility is warranted.</P>