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        A Water-saving Irrigation Decision-making Model for Greenhouse Tomatoes based on Genetic Optimization T-S Fuzzy Neural Network

        ( Zhili Chen ),( Chunjiang Zhao ),( Huarui Wu ),( Yisheng Miao ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.6

        In order to improve the utilization of irrigation water resources of greenhouse tomatoes, a water-saving irrigation decision-making model based on genetic optimization T-S fuzzy neural network is proposed in this paper. The main work are as follows: Firstly, the traditional genetic algorithm is optimized by introducing the constraint operator and update operator of the Krill herd (KH) algorithm. Secondly, the weights and thresholds of T-S fuzzy neural network are optimized by using the improved genetic algorithm. Finally, on the basis of the real data set, the genetic optimization T-S fuzzy neural network is used to simulate and predict the irrigation volume for greenhouse tomatoes. The performance of the genetic algorithm improved T-S fuzzy neural network (GA-TSFNN), the traditional T-S fuzzy neural network algorithm (TSFNN), BP neural network algorithm(BPNN) and the genetic algorithm improved BP neural network algorithm (GA-BPNN) is compared by simulation. The simulation experiment results show that compared with the TSFNN, BPNN and the GA-BPNN, the error of the GA-TSFNN between the predicted value and the actual value of the irrigation volume is smaller, and the proposed method has a better prediction effect. This paper provides new ideas for the water-saving irrigation decision in greenhouse tomatoes.

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        Genetic diversity and phylogenetic analysis of porcine reproductive and respiratory syndrome virus in southern China from 2007 to 2014

        Qiwen Wu,Zhili Li,Guanqun Zhang,Jianqiang Niu,Xiduo Zeng,Baoli Sun,Jingyun Ma 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.3

        Porcine reproductive and respiratory syndrome virus (PRRSV) has a high degree of genetic variation. In this study, we characterized the genetic variation and evolutionary relationships among circulating PRRSV strains in southern China. We analyzed 29 NSP2 strains and 150 ORF5 strains from clinical samples collected in southern China during 2007–2014. The alignment results showed that the nucleotide identity similarities of the two genes among these strains were 80.5%–99.7% and 80.9%–100%, respectively. Phylogenetic analysis based on the NSP2 gene showed that highly pathogenic (HP)-PRRSV was still the dominant virus in southern China from 2013 to 2014. Compared with reference strains CH-1a and VR-2332, the field strain 131101-GD-SHC, which shared high homology with JXA1-P170, had a novel 12 amino acid deletion at position 499-510. Phylogenetic analysis based on the ORF5 gene showed that HP-PRRSV, VR2332-like strains, and QYYZ-like strains were simultaneously circulating in southern China from 2007 to 2014, suggesting that, in recent years, the type 2 PRRSV was more diverse in southern China. In conclusion, mutations in the decoy epitope and primary neutralizing epitope could be markers of viral evolution and used to study evolutionary relationships among PRRSV strains in China.

      • KCI등재

        A Chlamydomonas reinhardtii nuclear-encoded MinE homologue recognizes the Escherichia coli division site, and the evolutionary implications of MinE gene transfer from chloroplast to nucleus

        Lei Wang,Zhili Wu,Linyan Li,Weizhong Liu,Yong Hu 한국식물학회 2017 Journal of Plant Biology Vol.60 No.2

        Escherichia coli MinE is required for placement of a division septum. A nucleus-encoded and plastidtargeted MinE homologue, CrMinE, was identified from Chlamydomonas reinhardtii. Similar to the Arabidopsis nucleus-encoded AtMinE, the CrMinE protein possesses extra N-terminal and C-terminal extensions relative to the eubacterial and other algal plastid-encoded MinE proteins. The CrMinE protein functions in plastids, as revealed by a transient expression assay using a full-length CrMinE protein fused to enhanced green fluorescent protein. In addition, the overexpressed CrMinE:EGFP in wild-type E. coli with clear coiled structures could still recognize the cell division site of the host cell, which suggests evolutionary conservation of the MinE mode of action. No MinE homologue was found in a search of all researched plastid genome sequences of land plants; moreover, the researched MinEs of land plants are encoded by nuclear genomes. Thus, the identification of the CrMinE located in the nuclear genome implies that the transfer events of MinE from plastid to nucleus might have occurred before the evolution of land plants and during the evolution of green algae.

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        FACILE SOLVOTHERMAL SYNTHESIS OF MESOSTRUCTURED CHITOSAN-COATED Fe 3 O 4 NANOPARTICLES AND ITS FURTHER MODIFICATION WITH FOLIC ACID FOR IMPROVING TARGETED DRUG DELIVERY

        MAO SHEN,CHENGLIN WU,WENPING JIA,CHENGHONG LI,ZHILI ZHANG,YANGMIN JIN,GUODONG FAN,CAIPING LIN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.7

        Mesostructured chitosan-coated Fe 3 O 4 nanoparticles (CS-coated Fe 3 O 4 NPs) were synthesizedby a facile one-step solvothermal method via using chitosan as a surface-modi¯cation agent. Subsequently, the surfaces of CS-coated Fe 3 O 4 NPs were successfully conjugated with folic acid(FA) molecules to obtain FA – CS-coated Fe 3 O 4 NPs for improving targeted drug delivery. Themorphology, chemical component and magnetic property of as-prepared composite nanoparticleswere characterized by Fourier transform infrared spectroscopy (FTIR), X-ray di®raction (XRD),dynamic light scattering (DLS), scanning transmission electron microscopy (SEM), transmissionelectron microscopy (TEM), thermal gravimetric analysis (TGA) and vibrating sample magne-tometer (VSM). Furthermore, doxorubicin hydrochloride (DOX) as a model drug was encap-sulated for investigating drug release pattern in vitro. The results show that the magnetizationsaturation value of FA – CS-coated Fe 3 O 4 NPs was about 28.5 emu/g, exhibiting super-paramagnetic properties and mesostructure. DOX could be loaded to FA – CS-coated Fe 3 O 4 NPswith high capacity about 27.9%, and the release rate of DOX could be adjusted by the pH value. This work demonstrates that the prepared magnetic nanoparticles have potential applications inthe treatment of cancer as targeting drug delivery carriers.

      • SCIESCOPUSKCI등재

        Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows

        Li, Han,Wang, Xiangming,Wu, Yan,Zhang, Dingran,Xu, Hongyang,Xu, Hongrun,Xing, Xiaoguang,Qi, Zhili Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.9

        Objective: Bedding materials directly contact hooves of dairy cows and they may serve as environmental sources of lameness-associated pathogen. However, the specific composition of bacteria hidden in bedding materials is still not clear. The aim of this study was to determine the effect bedding material and its bacterial composition has on lameness of Holstein heifers. Methods: Forty-eight Holstein heifers with similar body weights were randomly assigned into three groups including sand bedding (SB), concrete floor (CF), and compost bedding (CB). Hock injuries severity and gait performance of dairy cows were scored individually once a week. Blood samples were collected at the end of the experiment and bedding material samples were collected once a week for Illumina sequencing. Results: The CF increased visible hock injuries severity and serum biomarkers of joint damage in comparison to SB and CB groups. Besides, Illumina sequencing and analysis showed that the bacterial community of CB samples had higher similarity to that of SB samples than CF samples. Bacteria in three bedding materials were dominated by gastrointestinal bacteria and organic matter-degrading bacteria, such as Actinobacteria, Firmicutes, and norank JG30-KF-cM45. Lameness-associated Spirochaetaceae and Treponeme were only detected in SB and CB samples with a very low relative abundance (0% to 0.08%). Conclusion: The bacterial communities differed among bedding materials. However, the treponemes pathogens involved in the pathogenesis of lameness may not be a part of microbiota in bedding materials of dairy cows.

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