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Zhijun Zhou,Bei Guan,Jinyan Chai,Xuting Che 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
We used Illumina next-generation sequencing data to generate the nearly complete mitochondrial genomes (mitogenomes) of three phasmatodeans (Megalophasma granulata, Calvisia medogensis, and Phyllium tibetense) without the aid of additional techniques such as long-range PCR or cloning. The most surprising finding was the presence of a novel gene arrangement “trnR-trnA-trnN-trnSAGN-trnE-trnF” (genes underlined are encoded by the minority strand) that was detected in M. granulata. The ancestral order of this tRNA gene cluster is typically “trnA-trnR-trnN-trnSAGN-trnE-trnF”. However, trnA was inserted between trnR and trnN in M. granulata, and this represents a rare case of gene rearrangement in Phasmatodea. Phylogenetic analyses were conducted on the concatenated nucleotide sequences of all protein-coding genes and two ribosomal RNA genes. Both maximum likelihood and Bayesian analyses failed to support the monophyly of Areolatae, Anareolatae, Diapheromeridae, Phasmatidae, Heteropterygidae, Necrosciinae, and Necrosciini. However, the monophyly of several lower taxonomic groups were confirmed in our analysis. For instance, two Ramulus species and Entoria okinawaensis were recovered in the monophyletic Clitumnini, and Phraortes, Phyllium, and Bacillus species formed individual monophyletic clades. Our results support the hypothesis that wing-loss independently occurred several times in Verophasmatodea.
The nonlinear absorption of graphene oxide water solution in femtosecond regime
Lingling Ran,Zhijun Chai,Yachen Gao,Wenzhi Wu,Qing Chang,Degui Kong 한국물리학회 2016 Current Applied Physics Vol.16 No.9
The nonlinear absorption properties of graphene oxide water solution were investigated with femtosecond pulses using Z-scan and pump-probe techniques at 800 nm wavelength. The researching results indicated that the nonlinear absorption of graphene oxide water solution include three parts: twophoton absorption of bound electrons from valence band, excited state absorption of electrons from the low energy state in conduction band and the excited state absorption of electrons from the bottom of conduction band. By theoretically fitting the experimental results, we got the two-photon absorption coefficient about b ¼ 3 1014 m/W, and the two excited state absorption cross section in the order of 1020 m2 and 1021 m2 respectively. In addition, the excited state lifetime of electron on the low energy state of conduction band was obtained. The investigation indicated that graphene oxide water solution is a good nonlinear optical material.
Effects of β Air Cooling and Subsequent Cold Rolling on Microstructure and Hardness of Zr702 Sheet
Haotian Guan,Lingguo Zeng,Zhijun Li,Linjiang Chai,Yufan Zhu,Yueyuan Wang,Qin Huang,Ke Chen,Liang‑yu Chen,Ning Guo 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.2
In this work, a Zr702 sheet was subjected to β air cooling and then rolled to 15% reduction at room temperature, with theirdetailed microstructural characteristics characterized by electron channeling contrast imaging and electron backscatterdiffraction techniques. Results show that after the β air cooling, the prior equiaxed grains in the as-received material arecompletely transformed into Widmanstätten structures featured by coarse plates with typical phase transformation misorientationsbetween them. The subsequent 15% rolling allows both slip and twinning (especially the {10–12} type) to beactivated readily, leading to significant grain refinement and the appearance of misorientation angle peaks around 3°–5° and85°. Analyses on kernel average misorientations reveal that there exist very low residual strains in the β-air-cooled specimenwhile they are markedly increased after the 15% rolling and preferably distributed near low-angle and twin boundaries. Hardnessmeasurements show that the specimen hardness is evidently decreased from ~ 199 to ~ 170 HV after the β air cooling,which can be attributed to grain coarsening and the scattered orientations associated with the slow β → α transformation. For the 15%-rolled specimen, however, effective grain refinement by twinning and denser low-angle boundaries jointly leadto ~ 35% hardness increment to ~ 228 HV.
Hu Hongyan,Wang Xiaowei,Wu Zhijun,Chen Mo,Chai Tuanyao,Wang Hong 한국식물학회 2022 Journal of Plant Biology Vol.65 No.6
Plants have evolved complex signaling networks that enable them to adapt to adverse environmental conditions. The dehydration-responsive element-binding (DREB) transcription factors are important for plant responses to abiotic stresses. In this study, a new member of the AP2/ERF transcription factor gene family, PcDREB2A, was cloned and characterized from Polygonum cuspidatum, a traditional Chinese medicinal herb. PcDREB2A, which includes a typical AP2 domain, was clustered in the A-2 subgroup of the DREB subfamily. At the seedling stage, PcDREB2A expression was induced by cold, salt, and drought stresses. A yeast one-hybrid assay and an analysis of transiently transformed tobacco revealed that PcDREB2A can specifically bind to the DRE motif and transactivate reporter gene expression. Following 200 and 250 mM mannitol treatments, the PcDREB2A-overexpressing Arabidopsis thaliana lines had longer roots and a significantly higher fresh weight than the wild-type plants. Furthermore, under drought stress conditions, the PcDREB2A-overexpressing A. thaliana plants accumulated less malondialdehyde than the control plants. These results indicate that PcDREB2A encodes a novel DREB transcription factor in P. cuspidatum. Furthermore, the data generated in this study may be useful for researchers and breeders interested in genetically engineering plants to increase drought tolerance without inhibiting growth.