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Design of On-line UPS Based on Boost Converter
Chunyang Gu,Zhibing Yang,Chunling Yang,Mengda Yang 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
Design and build up a new on-line digital UPS based on boost topological structure with a new kind of single-chip computer, STC12C5412AD, realizing sine wave and square wave output, and shifting among 50Hz, 60 Hz, and 400Hz of the supply for adopting into different industrial standards and applications. Such UPS system contains the module of intelligent charging from storage battery and thorough security functions, characterized by the output of un-interruption and high stability, low distortion, free of interference, low cost and multi-function.
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The FvCYP714C2 gene plays an important role in gibberellin synthesis in the woodland strawberry
Xiaofan Guo,Zhibing Xie,Yang Zhang,Shouming Wang 한국유전학회 2021 Genes & Genomics Vol.43 No.1
Background Fragaria vesca, the woodland strawberry, is a diploid relative of the cultivated strawberry. A GA-defcient mutant was found in ethyl methanesulfonate (EMS)-mutagenized lines of the Fragaria vesca accession ‘Yellow Wonder’. Objective CYP714C2 was found to be diferentially expressed using RNA-seq analysis. It is necessary to identify the function of this gene. Methods In order to identify the function of this gene, it was cloned and transformed into Arabidopsis thaliana. Results The DNA sequence of CYP714C2 was found to be 1940 bp in length, with an open reading frame (ORF) of 1539 bp that is predicted to encode a protein of 512 amino acids. The hydrophilicity of this protein is low and it is unstable. The highest relative expression of FvCYP714C2 was found in the leaves, followed by the pedicels, and low expression levels were found in the other tissues examined. Constitutive expression of FvCYP714C2 signifcantly promoted the growth of transgenic A. thaliana plants; transgenic Arabidopsis plants grew faster and grew well than wild type Col-0 plants. GA1+3 contents of the genetically modifed Arabidopsis lines were signifcantly higher than that in the wild type. Conclusion We conclude that FvCYP714C2 is a gene that functions in the gibberellin biosynthesis pathway in strawberry.
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Yanwei Ji,Yang Xu,Yanping Li,Zhui Tu,Zhibing Huang,Xue Liu,Da Lei 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.1
The purpose of this study was to develop a membrane filtration method to isolate uninuclei conidium of Aspergillus oryzae, then the method was adopted to develop a transformation system of A. oryzae. A. oryzae 3.951 contained 1–4 nuclei in each conidium. The percentages of uninucleate and binucleate conidia were approximately 16.15 and 74.22%, respectively. Conidia suspension was filtrated with a 5-μm membrane to overcome the bottleneck caused by multinucleate conidia and to remove excess multinucleate conidia before UV mutagenesis. Uninucleate conidia of 5-fluoroorotic acid (5-FOA)-resistant strains were enriched by filtration with a 3-μm membrane. The pyrG mutant strain AS11 was obtained and GFP-pyrG was successfully transformed into AS11.
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