In Vitro Biological Characterization of DCUN1D5 in DNA Damage Response

Guo, Wei,Li, Guo-Jun,Xu, Hong-Bo,Xie, Jie-Shi,Shi, Tai-Ping,Zhang, Sheng-Zhong,Chen, Xiao-Hong,Huang, Zhi-Gang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: Novel prognostic biomarkers or therapeutic molecular targets for laryngeal squamous cell carcinoma (LSCC) are an urgent priority. We here sought to identify multiple novel LSCC-associated genes. Methods: Using high-density microarray expression profiling, we identified multiple genes that were significantly altered between human LSCCs and paired normal tissues. Potential oncogenic functions of one such gene, DCUN1D5, were further characterized in vitro. Results: Our results demonstrated that DCUN1D5 was highly expressed in LSCCs. Overexpression of DCUN1D5 in vitro resulted in 2.7-fold increased cellular migration, 67.5% increased invasive capacity, and 2.6-fold increased proliferation. Endogenous DCUN1D5 expression was decreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the number of cells in the S phase by 10.2% and increased apoptosis by 11.7%. Conclusion: Our data suggest that DCUN1D5 in vitro might have vital roles in DNA damage response, but further studies are warranted to assess its significance in vivo.

Humic Substances Act as Electron Acceptor and Redox Mediator for Microbial Dissimilatory Azoreduction by Shewanella decolorationis S12

( Yi Guo Hong ),( Guo Jun ),( Zhi Cheng Xu ),( Mei Ying Xu ),( Guo Ping Sun ) 한국미생물생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.3

Risk Factors for Early and Late Intrahepatic Recurrence in Patients with Single Hepatocellular Carcinoma Without Macrovascular Invasion after Curative Resection

Li, Shu-Hong,Guo, Zhi-Xing,Xiao, Cheng-Zuo,Wei, Wei,Shi, Ming,Chen, Zhi-Yuan,Cai, Mu-Yan,Zheng, Lie,Guo, Rong-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Background: Prognostic factors of postoperative early and late recurrence in patients with hepatocellular carcinoma (HCC) undergoing curative resection remain to be clarified. The aim of this study was to identify risk factors for postoperative early (${\leq}$ 2 year) and late (> 2 year) intrahepatic recurrences in patients with single HCCs without macrovascular invasion. Methods: A total of 280 patients from December 2004 to December 2007 were retrospectively included in this study. Intrahepatic recurrence was classified into early (${\leq}$ 2 year) and late (> 2 year) and the Chi-Square test or Fisher's exact test and multivariate logistic regression analysis were performed to determine significant risk factors. Results: During the follow-up, 124 patients had intrahepatic recurrence, early and late in 82 and 42 patients, respectively. Multivariate logistic regression analysis showed that microvascular invasion (p=0.006, HR: 2.397, 95% CI: 1.290-4.451) was the only independent risk factor for early recurrence, while being female (p = 0.031, HR: 0.326, 95% CI: 0.118-0.901), and having a high degree of cirrhosis (P=0.001, HR: 2.483, 95% CI: 1.417-4.349) were independent risk factors for late recurrence. Conclusions: Early and late recurrence of HCC is linked to different risk factors in patients with single HCC without macrovascular invasion. This results suggested different emphases of strategies for prevent of recurrence after curative resection, more active intervention including adjuvant therapy, anti-cirrhosis drugs and careful follow-up being necessary for patients with relevant risk factors.

Purification and Characterization of a β-Glucosidase from Aspergillus niger and Its Application in the Hydrolysis of Geniposide to Genipin

( Guo Hong Gong ),( Zhi Ming Zheng ),( Hui Liu,Li Wang ),( Jin Shan Diao ),( Peng Wang ),( Gen Hai Zhao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.6

An extracellular β-glucosidase from Aspergillus niger Au0847 was purified to homogeneity by precipitation with ammonium sulfate, anion exchange, and gel filtration. The purified protein was composed of two subunits with molecular masses of 110 and 120 kDa. Au0847 β-glucosidase exhibited relatively high thermostability and pH stability, and its highest activity was obtained at 65°C and pH 4.6, respectively. As a potential metalloprotein, its enzymatic activity was potently stimulated by manganese ion and DTT. The β-glucosidase displayed avid affinity and high catalytic efficiency for geniposide. Au0847 β-glucosidase has potential value as an industrial enzyme for the hydrolysis of geniposide to genipin.

Enzymatic Synthesis of Theanine with Escherichia coli γ-glutamyltranspeptidase from a Series of γ-glutamyl Anilide Substrate Analogues

Hong-juan Zhang,Wei-guo Zhang,Zhi-yuan Wang,Yue-ping Zhan,Li-sheng Xu,Jun-zhong Liu,Qian Liu,Qing-cai Jiao 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.2

In order to investigate the catalytic mechanism of Escherichia coli γ-glutamyltranspeptidase, ten para- and meta- substituted γ-glutamyl anilides were chemically prepared and employed as substrates to synthesize L-theanine to assay the activity of γ-glutamyltranspeptidase. The reaction was optimized for γ-glutamyl-p-nitroanilide. Key factors such as substrate specificity, pH, temperature, and the substrate mole ratio were all investigated. Kinetic studies of the acyl transfer reaction were described and the Hammett plot was constructed. This study indicated that the ratelimiting acylation reaction of γ-glutamyltranspeptidase can apparently be accelerated by either the electron-withdrawing or electron-donating substituents of γ-glutamyl anilides. The reaction could be catalyzed by the general acid and carboxy of Asp-433 or phenolic hydroxyl Tyr-444 may be the acid by autodock simulation for all prepared γ-glutamyl anilides.

Astilbin alleviates sepsis-induced acute lung injury by inhibiting the expression of macrophage inhibitory factor in rats

Hong-bo Zhang,Li-chao Sun,Li-da Zhi,Qian-kuan Wen,Zhi-wei Qi,Sheng-tao Yan,Wen Li,Guo-qiang Zhang 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.10

Sepsis is a systemic inflammatory responsesyndrome caused by severe infections. Astilbin is a dihydroflavonolderivative found in many medicinal and foodplants with multiple pharmacological functions. To investigatethe effects of astilbin on sepsis-induced acute lunginjury (ALI), cecal ligation and puncture was performed onrats to establish a sepsis-induced ALI model; these ratswere then treated with astilbin at different concentrations. Lung injury scores, including lung wet/dry ratio, proteinleakage, myeloperoxidase activity, and inflammatory cellinfiltration were determined to evaluate the effects ofastilbin on sepsis-induced ALI. We found that astilbintreatment significantly attenuates sepsis-induced lunginjury and improves survival rate, lung injury scores, lungwet/dry ratio, protein leakage, myeloperoxidase activity,and inflammatory cell infiltration. Astilbin treatment alsodramatically decreased the production of inflammatorycytokines and chemokines in bronchoalveolar lavage fluid. Further, astilbin treatment inhibited the expression andproduction of macrophage inhibitory factor (MIF), whichinhibits the inflammatory response. Collectively, these datasuggest that astilbin has a protective effect against sepsisinducedALI by inhibiting MIF-mediated inflammatoryresponses. This study provides a molecular basis for astilbinas a new medical treatment for sepsis-induced ALI.

Effects of Wushu-Sanda Player`s Different Exercise Intensity on the Fatigue Substance, Oxidative Stress and Antioxidant Enzyme

( Guo Jian He ),( Zhi Hong Bao ),( Tian Yu Liu ),( Shuai Li ),( Jeong Ryong Chae ) 한국체육학회 2016 국제스포츠과학 학술대회 Vol.2016 No.1

Purpose: The purpose of this study was designed to examine the activation of Fatigue Substance, Oxidative Stress and Antioxidant Enzyme in Wushu Sanda players according to the different exercise intensity. Methods & Materials: Subjects of this four-week-long Wushu exercise program were divided into 2 groups: high intensity group (n=8), and low intensity group (n=8). Subjects participated in this program once a day (high intensity group:60mins, low intensity group:90mins) and 5 times per week (Monday to Friday). Every subjects’ fatigue substance (LAC, LDH), oxidative stress (MDA) and antioxidant enzyme (GPx, SOD, CAT) were measured before the program, immediately after exercise, after 30mins recovery and after 60mins recovery. All these parameters were measured again four-week latter. Statistics: ANOVA, paired t-test and repeated ANOVA were carried out to analyze the changes before and after the exercises, changes in terms of fatigue substance, oxidative stress and antioxidant enzyme, respectively. Statistical significance is established at the .05 level (p<.05). Result: 1. Change of LAC and LDH Pre and Post exercises, analysis of the LAC have statistically significant changes in recovery 30 and 60minutes than immediately after within each group (p<.05). But according to the time of measurement there are no statistically significant difference (p>.05). And, after four weeks, LAC in both groups are lower than the concentration of before exercise. Especially, the low intensity exercise group are lower than the high intensity exercise group. But all variables are no statistically significant differences(p<.05). Pre and Post exercises, analysis of the LDH was statistically significant changes in immediately after and recovery 60minutes within each group(p<.05). And according to the time of measurement were only significantly difference in the rest (p<.05). What’s more, the high intensity exercise group are lower than the low intensity exercise group (p>.05). 2. Change of MDA MDA in low intensity group was significantly higher after exercise and 30mins recovery than before exercise (p<.05). In high intensity group, no significant difference was found. 3. Change of GPx, SOD, CAT After the four weeks exercise, GPx in high intensity group shows significant differences after 30mins and 60mins recovery compared with the value of pre-exercise (p<.05). No significant difference was found in low intensity group. Compared to the value of SOD pre-exercise, the SOD of post-exercise shows significant differences after 30mins and 60mins recovery in high intensity group ( p<.05) while only immediately after exercise in low intensity group (p<.01 ). In CAT, a significant difference was found in high intensity group after the four weeks exercise (p<.05). No significant difference was found in low intensity group. Conclusion: After four weeks exercise, some differences were found in fatigue substance(LAC, LDH), oxidative stress(MDA) and antioxidant enzyme(GPx, SOD, CAT) in both groups: LDH was found to be increased in high intensity group after the four-week exercise; MDA was increased in low intensity group after exercise and 30mins recovery than before exercise; compared with the value of pre-exercise, GPx and SOD in high intensity group shows significant differences after 30mins and 60mins recovery; while in low intensity group, the SOD difference only occurred immediately after exercise; In CAT, a significant difference was found in high intensity group but no significant difference was found in low intensity group.

Metastasis associated genomic aberrations in stage II rectal cancer

Hong Zhao,Zhi-Zhou Shi,Rui Jiang,Dong-Bing Zhao,Hai-Tao Zhou,Jian-Wei Liang,Xin-Yu Bi,Jian-Jun Zhao,Zhi-Yu Li,Jian-Guo Zhou,Zhen Huang,Ye-Fan Zhang,Jian Wang,Xin Xu,Yan Cai,Ming-Rong Wang,Yu Zhang 한국유전학회 2016 Genes & Genomics Vol.38 No.11

Genomic aberrations of rectal carcinoma, especially DNA copy number changes associated with metastasis were largely unclear. We aim to identify the metastasis associated biomarkers in stage II rectal cancer. Formalin-fixed, paraffin-embedded primary tumor tissues of stage II rectal carcinoma were analyzed by array-based comparative genomic hybridization, and genomic aberrations were identified by Genomic Workbench and SAM software. Copy number changes and mRNA expressions were validated by Real-time PCR in an independent rectal cancer samples. The results showed that the most frequent gains in stage II rectal cancer were at 1q21.2-q23.1, 3p21.31, 11q12.2-q23.3, 12q24.11-q24.31, 12q13.11-q14.1 and losses in 18q11.2-q23, 17q21.33-q22, 13q31.1-q31.3, 21q21.1-q21.3, 8p23.3-p23.1 and 4q22.1-q23. Twenty-two amplifications and five homozygous deletions were also identified. We further found that S100A1 (1q21.3-q23.1), MCM7 (7q22.1) and JUND (19p13.11) were amplified and overexpressed in stage II rectal cancer. Interestingly, the genomic aberrations affected 14 signaling pathways including VEGF signaling pathway and fatty acid metabolism. Most importantly, loss of 13q31.1-q34 and gain of 1q44 were associated with distant metastasis. Our results indicated that these metastasis associated genomic changes may be useful to reveal the pathogenesis of rectal cancer metastasis and identify candidate biomarkers.

Characteristics Comparison before and after Lyophilization of Transferrin Modified Procationic-Liposome-Protamine-DNA Complexes (Tf-PLPD)

Zhi-Rong Zhong,Zhi-rong Zhang,Ji Liu,Yong Deng,Hong-wei Zhang,Yao Fu,Qing-guo Song,Qin He 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.1

A novel non-viral gene delivery system, Procationic-Liposome-Protamine-DNA complexes (PLPD) which could further adsorb transferrin on the surface as a targeting ligand to form Tf- PLPD, was prepared and characterized before and after lyophilization. The size distribution of Tf-PLPD was in the range of 240 ± 12 nm and the zeta potential was -24.10 ± 2.5 mV. The transfection efficiencies of PLPD and Tf-PLPD were 12.18 ± 3.8 and 24.26 ± 2.6 mU β-galactosidase/ mg protein respectively. The lyophilization and the presence of serum didn’t affect the tansfectivities of PLPD or Tf-PLPD. Compared to LipofectamineTM 2000 (Invitrogen, U.S.A.), the procationic liposomes had less cytotoxicity to cells. In summary the procationic lipoplex described here, combining the condensing effect of protamine and the targeting capability of Tf, was a perspective non-viral vector for gene delivery system.

Development of Integrated Electro-Hydraulic Braking System and Its ABS Application

Zhi-Hui Tan,Zhen-Fu Chen,Xiao-Fei Pei,Xue-Xun Guo,Shuang-Hong Pei 한국정밀공학회 2016 International Journal of Precision Engineering and Vol.17 No.3

This paper presented a new type of integrated EHB system. The EHB consisted of a compact three-chamber structure of master cylinder and mode switching valves. This allowed for the easy implementation of three different modes: normal mode, failsafe mode and ABS mode. In normal mode, a PWM control method was proposed for pressure regulation that took into account overshoot and hysteresis. To provide a favorable pedal feeling, a pedal stroke simulator was designed and integrated with the master cylinder. The failsafe and ABS modes were verified and the performance of the EHB in the three modes was evaluated using co-simulation and a bench test.