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Molecular cloning of two novel stearoyl-acyl desaturase genes from winterness wheat
Zhi-Fu Guo,Feng-Zhen Li,Xiao-Gang Ma,Feng Lin,Hui Ma,Li-Jing Chen,Ming Zhong,Li-Ping Bai,Ying Yi 한국유전학회 2011 Genes & Genomics Vol.33 No.5
Using RT-PCR and rapid amplification of cDNA ends, two new full-length cDNAs of SAD (TaSAD1 and TaSAD2) were obtained from a hardiest winter wheat cultivar (Mironovskaya808). Sequence comparison analysis showed that the deduced amino acid sequences of TaSAD1 and TaSAD2 had high similarity to those of other reported SAD proteins. They were also different each other by some substitutions, insertions and/or deletions involving single amino acid residues or motifs. Based on evolution analysis, it was clear that all SAD genes from Poaceae were closer than those from other genus such as Arabidopsis,Glycine, Triadica, Brassica, Sesamum and Bassia. All SAD genes clustered into two major groups in Poaceae. Meanwhile,TaSAD1 and TaSAD2 were clustered into different groups. The tertiary structure prediction indicated that both TaSAD1 and TaSAD2 proteins were a compact globular protein and their model structures almost were the same.
Fu, Chun-Jing,Pan, Xiong-Fei,Zhao, Zhi-Mei,Saheb-Kashaf, Michael,Chen, Feng,Wen, Ying,Yang, Chun-Xia,Zhong, Xiao-Ni Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15
Objectives: To evaluate medical students' knowledge of HPV and HPV related diseases and assess their attitudes towards HPV vaccination. Methods: A total of 605 medical undergraduates from Chongqing Medical University in China were surveyed using a structured and pretested questionnaire on HPV related knowledge. Results: Some 68.9% of the medical students were females, and mean age was 21.6 (${\pm}1.00$) years. Only 10.6% correctly answered more than 11 out of 14 questions on HPV related knowledge, 71.8% being willing to receive/advise on HPV vaccination. Female students (OR: 2.69; 95% CI: 1.53-4.72) and students desiring more HPV education (OR: 4.24; 95% CI: 1.67-10.8) were more willing to accept HPV vaccination. HPV vaccination acceptability was observed to show a positive association with HPV related knowledge. Conclusions: Our survey found low levels of HPV related knowledge and HPV vaccination acceptability among participating medical students. HPV education should be systematically incorporated into medical education to increase awareness of HPV vaccination.
Feng Chen,Zhi Chen,Hai-Hong Zhu,Lin-Fu Zhou,Shan-Shan Wu,Jing Wang 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.7
The scaffold protein IQGAP1 shows elevated levels in several cancer types, but its expression in hepatocellular carcinoma is unknown. We found that 58%of human hepatocellular carcinoma tissue samples had increased IQGAP1 expression compared to adjacent normal tissue. Overexpressing IQGAP1 raised the in vivo tumorigenicity of hepatocellular carcinoma cells, and forced overexpression of IQGAP1 in vitro stimulated cell proliferation. Cell growth was reduced by knockdown or mutation of IQGAP1, or by treatment of cells with a phosphotidylinositol 3-kinase inhibitor. To determine the mechanism by which IQGAP1 overexpression affected hepatocellular carcinoma cells,we confirmed its interaction in these cells with mammalian target of rapamycin (mTOR), a serine/threonine kinase that integrates signals about nutrient and energy status with downstream effectors that influence cell division. In addition, we discovered a new interaction involving IQGAP1, mTOR and Akt, which is a downstream target of mTOR. Akt phosphorylation on Ser-473, which is catalyzed by mTOR and required for Akt activation, increased with increasing amounts of IQGAP1, and decreased with IQGAP1 mutation. We hypothesize that IQGAP1 is a scaffold that facilitates mTOR and Akt interaction.
Selective Removal of Al(III) from Rare Earth Solutions Using Peas-based Activated Carbon
Fu-Qiang An,Rui-Yan Wu,Zhi-Guo Yuan,Tuo-Ping Hu,Jian-Feng Gao 대한화학회 2017 대한화학회지 Vol.61 No.5
Efficiently removing Al(III) from rare earth is very significant because even trace amount of Al(III) can cause serious harm to the rare earth materials. In this paper, a nitrogen-containing activated carbon, AC-P700, was synthesized using peas as raw materials. The AC-P700 was characterized by surface area analyzer, FT-IR, and XPS methods. The adsorption and recognition properties of AC-P700 towards Al(III) were investigated, and the recognition mechanism was also analyzed. The BET special surface area of AC-P700 was 1277.1 m2·g−1, and the average pore diameter was 1.90 nm. The AC-P700 possesses strong adsorption affinity and excellent recognition selectivity towards Al(III). The adsorption capacity for Al(III) could reach to 0.53 mmol·g−1, and relative selectivity coefficients relative to La(III) and Ce(III) is 9.6 and 8.7, respectively. Besides, ACP700 possesses better regeneration ability and reusability.
( Zhi-ke Liu ),( Qiu-yu Zhang ),( Ning-ning Yang ),( Ming-guo Xu ),( Jin-feng Xu ),( Ming-long Jing ),( Wen-xing Wu ),( Ya-dong Lu ),( Feng Shi ),( Chuang-fu Chen ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3
Salmonellosis is a highly contagious bacterial disease that threatens both human and poultry health. Tests that can detect Salmonella in the field are urgently required to facilitate disease control and for epidemiological investigations. Here, we combined loop-mediated isothermal amplification (LAMP) with a chromatographic lateral flow dipstick (LFD) to rapidly and accurately detect Salmonella. LAMP primers were designed to target the Salmonella invA gene. LAMP conditions were optimized by adjusting the ratio of inner to outer primers, MgSO<sub>4</sub> concentration, dNTP mix concentration, amplification temperature, and amplification time. We evaluated the specificity of our novel LAMP-LFD method using six Salmonella species and six related non-Salmonella strains. All six of the Salmonella strains, but none of the non-Salmonella strains, were amplified. LAMP-LFD was sensitive enough to detect concentrations of Salmonella enterica subsp. enterica serovar Pullorum genomic DNA as low as 89 fg/μl, which is 1,000 times more sensitive than conventional PCR. When artificially contaminated feed samples were analyzed, LAMP-LFD was also more sensitive than PCR. Finally, LAMP-LFD gave no false positives across 350 chicken anal swabs. Therefore, our novel LAMP-LFD assay was highly sensitive, specific, convenient, and fast, making it a valuable tool for the early diagnosis and monitoring of Salmonella infection in chickens.
RNA Interference-Based Transgenic Maize Resistant to Maize Dwarf Mosaic Virus
Zhi-Yong Zhang,Feng-Ling Fu,Lin Gou,Han-Guang Wang,Wan-Chen Li 한국식물학회 2010 Journal of Plant Biology Vol.52 No.4
Maize dwarf mosaic virus (MDMV) is a widespread pathogenic virus that causes serious loss of yield in maize (Zea mays). RNA interference (RNAi) triggered by hairpin RNA (hpRNA) transcribed from a transgenic inverted-repeat sequence is an effective way to defend against viruses in plants. In this study, an hpRNA expression vector containing a sense arm and an antisense arm of 150 bp separated by an intron of the maize actin gene was constructed to target the P1 protein (protease)gene of MDMV and used to transform Agrobacterium tumefaciens strain EHA105. The transformed Agrobacterium strain was used to transform maize embryonic calli isolated from immature embryos by an improved culture technique. In all, 46 plants were regenerated after stringent hygromycin B selection, and 18 of them were certified to be positive by PCR amplification. Of these positive plants,13 were grown to produce offspring, and nine were identified by Southern blotting to have the transgene integrated with one or two copies. The resistance of three T2 lines was evaluated in a field trial of dual MDMV inoculation in two environments and was found to be improved compared with the non-transformed control. The disease indexes of the transgenic plant lines h2, 13, and h1were not significantly different from the highly resistant control line H9-21. The viral titers of the inoculated plants were detected by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), and the result was in accord with the resistance evaluated in the field trial. The addition of uniconazole S3307 (0.25 mgl−1) and ABT rootpromoting powder (0.5 mgl−1) showed a significant improvement of hardening in regenerated plantlets, which were stronger and generated a better fibrous root system than the control. This improvement could facilitate the transgenic operation of maize.
Selective Removal of Al(III) from Rare Earth Solutions Using Peas-based Activated Carbon
An, Fu-Qiang,Wu, Rui-Yan,Li, Min,Yuan, Zhi-Guo,Hu, Tuo-Ping,Gao, Jian-Feng Korean Chemical Society 2017 대한화학회지 Vol.61 No.5
Efficiently removing Al(III) from rare earth is very significant because even trace amount of Al(III) can cause serious harm to the rare earth materials. In this paper, a nitrogen-containing activated carbon, AC-P700, was synthesized using peas as raw materials. The AC-P700 was characterized by surface area analyzer, FT-IR, and XPS methods. The adsorption and recognition properties of AC-P700 towards Al(III) were investigated, and the recognition mechanism was also analyzed. The BET special surface area of AC-P700 was $1277.1m^2{\cdot}g^{-1}$, and the average pore diameter was 1.90 nm. The AC-P700 possesses strong adsorption affinity and excellent recognition selectivity towards Al(III). The adsorption capacity for Al(III) could reach to $0.53mmol{\cdot}g^{-1}$, and relative selectivity coefficients relative to La(III) and Ce(III) is 9.6 and 8.7, respectively. Besides, AC-P700 possesses better regeneration ability and reusability.
Chen, Feng,Zhu, Hai-Hong,Zhou, Lin-Fu,Wu, Shan-Shan,Wang, Jing,Chen, Zhi Korean Society for Biochemistry and Molecular Bion 2010 Experimental and molecular medicine Vol.42 No.7
The scaffold protein IQGAP1 shows elevated levels in several cancer types, but its expression in hepatocellular carcinoma is unknown. We found that 58% of human hepatocellular carcinoma tissue samples had increased IQGAP1 expression compared to adjacent normal tissue. Overexpressing IQGAP1 raised the in vivo tumorigenicity of hepatocellular carcinoma cells, and forced overexpression of IQGAP1 in vitro stimulated cell proliferation. Cell growth was reduced by knockdown or mutation of IQGAP1, or by treatment of cells with a phosphotidylinositol 3-kinase inhibitor. To determine the mechanism by which IQGAP1 overexpression affected hepatocellular carcinoma cells, we confirmed its interaction in these cells with mammalian target of rapamycin (mTOR), a serine/threonine kinase that integrates signals about nutrient and energy status with downstream effectors that influence cell division. In addition, we discovered a new interaction involving IQGAP1, mTOR and Akt, which is a downstream target of mTOR. Akt phosphorylation on Ser-473, which is catalyzed by mTOR and required for Akt activation, increased with increasing amounts of IQGAP1, and decreased with IQGAP1 mutation. We hypothesize that IQGAP1 is a scaffold that facilitates mTOR and Akt interaction.
Zeng Bin,Feng Ze-Rui,Liu Ya-Ting,Chen Fu-Rong,Wang Shuai,Wu Shun-Fan,Yu Zhi-Tao,Gao Cong-Fen 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.4
Cap ‘n’ collar C (CncC), a transcription factor, plays a vital role in the development of insect resistance by regulating the expression of multiple detoxifying genes. Clarifying the molecular characteristics of CncC and its pathway regulating insecticide resistance will aid the development of integrated pest control strategies. Here we cloned and identified the molecular feature of CncC genes from the brown planthopper (BPH), Nilaparvata lugens (Stål), (Hemiptera Delphacidae). The full-length open reading frame of NlCncC was 3015 nucleotides with 1005 amino acids. The deduced amino acid sequence has a high similarity with other insect homologs and contains the characteristic Cnc/bZip domain architecture. Functional studies showed that silencing NlCncC by RNA interference (RNAi) could downregulate the transcript expression of NlCYP6ER1, NlCYP6CW1, and NlCYP6AY1, which increase the susceptibility to imidacloprid in N. lugens. The identification of molecular characteristics of NlCncC and func tional studies in this paper will contribute to designing effective control strategies for N. lugens.