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Physiological responses and small RNAs changes in maize under nitrogen deficiency and resupply
Zhenchao Yang,Zhengyan Wang,Chengcheng Yang,Zhao Yang,Hongquan Li,Yongjun Wu 한국유전학회 2019 Genes & Genomics Vol.41 No.10
Background Maize is an important crop in the world, nitrogen stress severely reduces maize yield. Although a large number of studies have identified the expression changes of microRNAs (miRNAs) under N stress in several species, the miRNAs expression patterns of N-deficient plants under N resupply remain unclear. Objective The primary objective of this study was to identify miRNAs in response to nitrogen stress and understand relevant physiological changes in nitrogen-deficient maize after nitrogen resupply. Methods Physiological parameters were measured to study relevant physiological changes under different nitrogen conditions. Small RNA sequencing and qRT-PCR analysis were performed to understand the response of miRNAs under different nitrogen conditions. Results The content of chlorophyll, soluble protein and nitrate nitrogen decreased than CK by 0.52, 0.49 and 0.82 times after N deficiency treatment and increased than ND by 0.52, 1.36 and 0.65 times after N resupply, respectively. Conversely, the activity of superoxide dismutase (SOD) and peroxidase (POD) increased by 0.67 and 1.64 times than CK after N deficiency, respectively, and decreased by 0.09 and 0.35 times than ND after N resupply. A total of 226 known miRNAs were identified by sRNA sequencing; 106 miRNAs were differentially expressed between the control and N-deficient groups, and 103 were differentially expressed between the N-deficient and N-resupply groups (P < 0.05). Real-time quantitative PCR (qPCR) was used to further validate and analyze the expression of the identified miRNAs. A total of 1609 target genes were identified by target prediction, and some differentially expressed miRNAs were predicted to target transcription factors and functional proteins. Gene Ontology (GO) analysis was used to determine the biological function of these targets and revealed that some miRNAs, such as miR169, miR1214, miR2199, miR398, miR408 and miR827 might be involved in nitrogen metabolism regulation. Conclusion Our study comprehensively provides important information on miRNA functions and molecular mechanisms in response to N stress. These findings may assist to improve nitrogen availability in plants.
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Miao Shiyuan,Yang Binbin,Wang Suisui,Wang Zhengyan,Lu Yujie 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.4
Liposcelis entomophila is a nuisance pest that seriously threatens the safety of stored grains owing in part of pesticide resistance. To facilitate better control of L. entomophila, future studies need to focus on gene function characterization. This requires the identification of suitable reference genes (RGs) for reverse-transcription quantitative PCR (RT-qPCR), which is crucial for the normalization of target gene expression. Here, we evalu ated the expression stability of 12 candidate RGs in L. entomophila across six experimental conditions (devel opmental stage, population, body part, phosphine fumigation, hypoxia induction, and temperature treatment). Under developmental conditions, ACTB, HSP70, and RPL23 were the most stable RGs. Among the different populations and body parts, RPL23, EF1a, and ACTB were the most stable RGs. Comparison of gene expression among adults under different environmental conditions revealed that at least seven RGs were fit for normalizing gene expression data. Among them, RPL23, EF1a, and ACTB showed the best fit as RGs in RT-qPCR analysis. After validation, our results demonstrated that EF1a and ACTB, as well as their combination, were the ideal RGs for L. entomophila across various experimental conditions. This study provides a comprehensive RG selection sug gestion for gene expression studies of L. entomophila and will facilitate future functional genomic research on the development and tolerance of environmental factors in psocids.
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Numerical Simulating Long-Distance Emergency Rescue System for Belt Fire in Coal Mine
Shao Hao,Jiang Shuguang,Wu Zhengyan,Zhang Weiqing,Wang Kai 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.12
The belt fire in coal mine has the characteristics of fast burning velocity, not easily extinguishing a fire and generating a great deal of poisonous and toxic gas. Once the belt fire happens, it will result in a large number of casualties and enormous property loss, which is one of the greatest disasters that influence on the safety production in coal mine. To reduce the number of casualties and property loss caused by belt fire, the long-distance emergency rescue system for belt fire is proposed, and used to directly import the poisonous and toxic gas produced by belt lane into return airway in order to guarantee the safety of staffs working in mining area. In order to research on how the efficiency of using the system and what the system has additional impact on the ventilation system, the present study applies the approach of numerical stimulation to establish the two-dimensional model of ventilation system. After the belt fire happens, and then this paper researches on the flow laws of poisonous and toxic gas, and explores the long-distance emergency rescue system for belt fire influencing on the ventilation system and controlling the poisonous and toxic gas. The current study yields the conclusions as follows: (1) how poisonous and toxic gas produced by belt fire in different ventilation models spreads and moves in the roadway; and (2) how the long-distance emergency rescue system for belt fire influences on the ventilation system. This research has certain instructive significance and reference value for improving the level of emergency rescue about the belt fire, and reducing the number of casualties and property loss caused by belt fire.
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