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Zhaoyang Qi,Yan Wang,Changshen Ye,Jie Chen,Ting Qiu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.111 No.-
The effectiveness of the catalytic oxidative desulfurization (CODS) process crucially depends on the catalyticactivity of the catalyst and the utilization of oxidizing agent. Catalyst with multiple active sites is aneffective method to improve the CODS effectiveness. The Zr(IV) and –COOH-containing metal–organicframework, UiO-66-(COOH)2, was synthesized and used as an active and recyclable catalyst for CODS. The UiO-66-(COOH)2 shows higher catalytic activity and H2O2 utilization in the CODS process thanUiO-66/–COOH, UiO-66, and H2BDC. And the high catalytic performance is derived from the synergisticeffect between the Zr(IV) open site and free –COOH functionality within the framework. Experimentalanalysis and DFT calculations results indicate that the synergistic effect, which changes the reaction pathand protects the *O2 – radical, is a very effective strategy to promote desulfurization efficiency and the utilizationefficiency of H2O2. In addition, it is essential to highlight that the active-site distance also has asignificant influence on the effectiveness of CODS. This research may provide a new avenue for improvingthe CODS performance.
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Fei Chen,Tianci Kou,Li Fan,Yan Zhou,Zhaoyang Ye,Liang Zhao,Wen-Song Tan 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.6
Cell cultures containing 0 ~ 5 mM sodium butyrate (NaBu) and grown at 30 and 37°C were conducted to investigate the combined effect of NaBu and low temperature on the quantity and quality of an antibody production in CHO cells. Although NaBu addition decreased cell viability by apoptosis in a dose-dependent manner at both 30 and 37°C, the onset of significant apoptosis induced by NaBu was delayed by lowering culture temperature. The highest specific antibody productivity (q_p) of 23.26 pg/cell/day was obtained in the culture containing 2 mM NaBu at 30°C; however, the highest antibody concentration of 167.84 mg/L was achieved in the culture containing 1 mM NaBu at 30°C, as the detrimental effect of further NaBu addition on cell growth compromised its beneficial effect on q_p. Moreover, protein quality with respect to the total sialic acid content and Nglycolylneuraminic acid (Neu5Gc) level was evaluated. There were no apparent changes regarding the total sialic acid content of the antibody, but manipulation of cultures with NaBu treatment or (and) low culture temperature did decrease Neu5Gc levels by 5 ~ 10%. Biological activity of the antibody was also assessed, and no obvious changes were observed. Collectively, the simultaneous application of NaBu and low culture temperature was an effective way to extend culture period and enhance final antibody concentration,without compromising the sialic acid content or biological activity.
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Lei Xu,Yuxi Wu,Yanli Liu,Yan Zhou,Zhaoyang Ye,Wen-Song Tan 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.1
Coculture between mesenchymal stem cells (MSCs) and chondrocytes has significant implications in cartilage regeneration. However, a conclusive understanding remains elusive. Previously, we reported that rabbit bone marrowderived MSCs (rbBMSCs) could downregulate the differentiated phenotype of rabbit articular chondrocytes (rbACs) in a non-contact coculture system for the first time. In the present study, a systemic investigation was performed to understand the biological characteristics of chondrocytes in coculture with MSCs. Firstly, cells (MSCs and chondrocytes) from different origins were cocultured in transwell system. Different chondrocytes, when cocultured with different MSCs respectively, consistently demonstrated stimulated proliferation, transformed morphology and declined glycosaminoglycan secretion of chondrocytes. Next, cell surface molecules and the global gene expression of rbACs were characterized. It was found that cocultured rbACs showed a distinct surface molecule profile and global gene expression compared to both dedifferentiated rbACs and rbBMSCs. In the end, cocultured rbACs were passaged and induced to undergo the chondrogenic redifferentiation. Better growth and chondrogenesis ability were confirmed compared with control cells without coculture. Together, chondrocytes display comprehensive changes in coculture with MSCs and the cocultured rbACs are beneficial for cartilage repair.
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