Three species of the family Noctuidae (Lepidoptera) new to China

Hui-Lin HAN,Zhao-Yao DUAN,Zhi-Wei FENG2 한국곤충학회 2008 Entomological Research Vol.38 No.3

Three species of the family Noctuidae –- Phlogophora striatovirens (Moore, 1867), Usbeca kulmburgi (Rebel, 1918) and Feliniopsis leucostigma (Moore, 1867) –- are reported for the first time from China. Photographs of adults and genital characteristics are provided.

Optimization of Reference Genes for Normalization of the Quantitative Polymerase Chain Reaction in Tissue Samples of Gastric Cancer

Zhao, Lian-Mei,Zheng, Zhao-Xu,Zhao, Xiwa,Shi, Juan,Bi, Jian-Jun,Pei, Wei,Feng, Qiang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

For an exact comparison of mRNA transcription in different samples or tissues with real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR), it is crucial to select a suitable internal reference gene. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and beta-actin (ACTB) have been frequently considered as house-keeping genes to normalize for changes in specific gene expression. However, it has been reported that these genes are unsuitable references in some cases, because their transcription is significantly variable under particular experimental conditions and among tissues. The present study was aimed to investigate which reference genes are most suitable for the study of gastric cancer tissues using qRT-PCR. 50 pairs of gastric cancer and corresponding peritumoral tissues were obtained from patients with gastric cancer. Absolute qRT-PCR was employed to detect the expression of GAPDH, ACTB, RPII and 18sRNA in the gastric cancer samples. Comparing gastric cancer with corresponding peritumoral tissues, GAPDH, ACTB and RPII were obviously upregulated 6.49, 5.0 and 3.68 fold, respectively. Yet 18sRNA had no obvious expression change in gastric cancer tissues and the corresponding peritumoral tissues. The expression of GAPDH, ${\beta}$-actin, RPII and 18sRNA showed no obvious changes in normal gastric epithelial cells compared with gastric cancer cell lines. The carcinoembryonic antigen (CEA), a widely used clinical tumor marker, was used as a validation gene. Only when 18sRNA was used as the normalizing gene was CEA obviously elevated in gastric cancer tissues compared with peritumoral tissues. Our data show that 18sRNA is stably expressed in gastric cancer samples and corresponding peritumoral tissues. These observations confirm that there is no universal reference gene and underline the importance of specific optimization of potential reference genes for any experimental condition.

A Novel Multi-Wing Chaotic System and Circuit Simulation

Zhao Wei Feng,Huang Yun 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.7

Base on a 3-D chaotic system, a new chaotic system generating three-wing and fourwing chaotic attractors is constructed by introducing a linear term. The phase diagrams, Lyapunov exponent spectrums and bifurcation diagrams of the system are studied by numerical simulation. Furthermore, the system is simulated by circuit, and the three- wing and four-wing chaotic attractors are observed. The circuit simulation results are in agreement with numerical simulation results.

Lobaplatin Combined Floxuridine/Pirarubicin-based Transcatheter Hepatic Arterial Chemoembolization for Unresectable Primary Hepatocellular Carcinoma

Zhao, Chang,Wang, Xu-Jie,Wang, Song,Feng, Wei-Hua,Shi, Lei,Yu, Chun-Peng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Purpose: To assess the effect and safety of lobaplatin combinated floxuridine /pirarubicin in transcatheter hepatic arterial chemoembolization(TACE) of unresectable primary liver cancer. Patients and Methods: TACE combined with the chemotherapy regimen was used to treat 34 unresectable primary liver cancer patients. DSA/MRI/CT/blood routine examinations were used to evaluate short term activity and toxicity after 4-5 weeks, the process being repeated if necessary. Results: Among the 34 cases, 1 (2.9%) showed a complete response, 21 (61.7%) a partial response, 8 (23.5%) stable disease, and 4 progressive disease, with a total effective rate of 67.6%. The content of alpha fetoprotein dropped by over 50% in 20 cases (58.8%). The rate of recovery was hepatalgia (88.2%), ascites (47.1%), appetite (55.9%), Performance Status(30.4%). The median follow-up time (MFT) was 281 days (63-558 days), and median progression-free survival was 118.5 days (95%, CI:88.8-148.2days). Adverse reactions (III-IV grade) were not common, with only 4 cases of vomiting and 2 cases of thrombocytopenia (III grade). Conclusions: Lobaplatin-based TACE is an effective and safe treatment for primary liver cancer.

Prepartum body condition score affects milk yield, lipid metabolism, and oxidation status of Holstein cows

Wei Zhao,Xue Chen,Jun Xiao,Xiao Hui Chen,Xue Feng Zhang,Tao Wang,Yu Guo Zhen,Gui Zin Qin 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.12

Objective: This study aimed to investigate the effects of prepartum body condition score (BCS) on the milk yield, lipid metabolism, and oxidative status of Holstein cows. Methods: A total of 112 multiparous Holstein cows were divided into 4 groups according to the BCS at 21 days before calving: medium BCS (3.0 to 3.25, MBCS), high BCS (3.5 to 3.75, HBCS), higher BCS (4.0 to 4.25, HerBCS), and highest BCS (4.5 to 5.0, HestBCS). Blood samples were collected on 21, 14, and 7 days before calving (precalving), on the calving day (calving), and on 7, 14, and 21 days after calving (postcalving). The indices of lipid metabolism and oxidative status were analyzed using bovine-specific enzyme-linked immunosorbent assay kit. Colostrum were taken after calving and analyzed by a refractometer and milk analyzer. The individual milk yield was recorded every 3 days. Results: The density and levels of immune globulin and lactoprotein of colostrum from Holstein cows in the HestBCS group were the highest (p<0.05). These animals not only had the highest (p<0.05) levels of serum non-esterified fatty acids and beta-hydroxybutyrate, but also had the highest (p<0.05) levels of malondialdehyde, superoxide dismutase, catalase, vitamin A, and vitamin E. In addition, greater (p<0.05) BCS loss was observed in the HestBCS cows. Conclusion: This study demonstrates that the milk yield, lipid metabolism, and oxidative status of Holstein cows are related to prepartum BCS and BCS loss during the transition period. HestBCS cows are more sensitive to oxidative stress and suffer greater loss of BCS after calving, whereas the MBCS animals had better milk yield performance.

Universal transport properties of a quantum dot system with a laterally-coupled Majorana zero mode

Wei-Jiang Gong,Ying Zhao,Zhen Gao,Shu-Feng Zhang 한국물리학회 2015 Current Applied Physics Vol.15 No.4

We present a comprehensive analysis about the transport properties of a quantum dot (QD) system with a side-coupled Majorana zero mode. Our calculation result shows that when the coupling manners between the two leads and QDs are identical, the local Andreev reflection and the interlead normal tunneling have the same magnitude at the zero-bias limit. Accordingly, the zero-bias conductance value is always equal to e2/2h, which is exactly one half of the resonant-tunneling conductance. This result is independent of the level number and the level distribution in the single-QD case, and in the coupled-QD case it is irrelevant to the geometry of the QD molecule. The universal transport property is a powerful evidence for the feasibility to detect the MBSs based on a QD circuit. This result also means that the QD condition is not a key factor to achieve the detection. On the other hand, if the decoupling phenomenon appears, the Majorana zero mode may play a trivial role in contributing to the conductance property.

A novel and simple approach for the synthesis of Fe3O4-graphene composite

Feng-Jun Zhang,Jin Liu,장칸,Wei Zhao,장원권,오원춘 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.8

High crystalline Fe3O4-graphene composite has been successfully synthesized via one-step thermolysis reaction. The results demonstrated that the attachment of iron-organic complex with graphene oxide sheets can facilely lead to magnetic graphene composites with a time-dependent calcination process. The composite was characterized by scanning electron microscopy, transmission electron microscopy, Raman spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The high efficient removal of methylene blue determined that Fe3O4-graphene is a promising sorbent material for wastewater treatment.

Study on host plants for reproduction of Chilo suppressalis

Wei-Hua Jiang,Hai-Dong Li,Xiong-Feng Cheng,Jian-Ren Ye,Yong-Bin Feng,Zhao-Jun Han 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.3

The rice stem borer, Chilo suppressalis, is generally considered to be a polyphagous pest. The current study challenges this view by investigating its oviposition preference, larval survival and development on different host plants under both laboratory and field conditions. Rice and water-oat populations of the borer inhabiting on corresponding plants respectively have partial reproductive isolation based on previous studies. In a laboratory multiple-choice test, C. suppressalis adults from rice population laid most of eggs on water-oat (46.5% of total eggs) and rice (43.8%), with very fewlaid onwheat (3.6%), sugarcane (4.0%) andmaize (2.0%). Field surveys supported the laboratory study and found no egg on the plants other than rice and water-oat. Neonate inoculation experiments performed in field and laboratory showed that larval survival rate was much higher on rice (49.1%–51.2%) and wheat (36.5%–44.1%) than that on water-oat (10.7%–10.8%), maize (1.2%–7.2%), sugarcane (0–1.5%) and weeds (2.4%). These results were discussed with the data reported from water-oat population and it was concluded that C. suppressalis is not a typical polyphagous pest. Rice population mainly reproduces on rice and use water-oat only as minor host, and water-oat population breeds better on water-oat than on rice as reported. Neither population could thrive on the other recorded host plants, which are used for the supplementary nutrition sources of larvae. These findings provide useful information for the development of control strategies to prevent C. suppressalis laying eggs on rice seedlings in early spring, hence effectively reducing population density of this pest in rice fields.

Antiproliferative Effects of Celecoxib in Hep-2 Cells through Telomerase Inhibition and Induction of Apoptosis

Zhao, Yong-Qiang,Feng, Hui-Wei,Jia, Tao,Chen, Xue-Mei,Zhang, Hui,Xu, An-Ting,Zhang, Hai-Ling,Fan, Xian-Liang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: To investigate the effect of celecoxib on telomerase activity and apoptosis in a human laryngeal squamous carcinoma cell line (Hep-2 cells). Materials and Methods: The growth inhibition rate of Hep-2 cells in vitro was measured by MTT assay, and apoptosis by TUNEL assay and flow cytometry (FCM). The TRAP-ELISA method was used to determine telomerase activity in Hep-2 cells. The mRNA expression of human telomerase RNA component(hTR), human telomerase reverse transcriptase (hTERT) and human telomerase-associated protein(hTEP1) was determined by RT-PCR assay. Expression of Bax and Bcl-2 proteins was assessed by Western blotting. Results: Celecoxib can inhibit proliferation and induce apoptosis in a dose- and time-dependent manner, repress telomerase activity, decrease hTERT mRNA and Bcl-2 protein expression and increase Bax protein expression, PGE2 had no effect on telomerase. Conclusions: Celecoxib had the antiproliferative and pro-apoptotic effect in Hep-2 cells. Apoptosis was accompanied by a decrease in telomerase activity which was directly correlated with hTERT mRNA and up-regulation of Bax/Bcl-2. Bcl-2 may thus play an important role in telomerase activity as well as apoptosis.

One Improved RLWE-based FHE and Fast Private Information Retrieval

( Wei-tao Song ),( Bin Hu ),( Xiu-feng Zhao ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.12

With the rapid development of cloud computing, it raises real questions on privacy protection, which greatly limits the use of cloud computing. However, fully homomorphic encryption (FHE) can make cloud computing consistent with privacy. In this paper, we propose a simpler FHE scheme based on ring LWE problem, with a smaller size of ciphertext and a lower noise-expansion factor for homomorphic multiplication. Then based on our optimized RLWE-based FHE scheme, we propose a fast single-database private information retrieval protocol, combining with batching and number theoretic transform technology.