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Qiao Peitong,Mei Xianghan,Li Ruixiang,Zhao Qiaoling,Xia Dingguo,Qiu Zhiyong,Shen Dongxu 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.4
Peptidoglycan recognition proteins (PGRPs) discriminate and bind peptidoglycans by acting as pattern recog nition receptors (PRRs) in insects, and function pivotal roles in innate immune response. In the present study, we cloned a full-length PGRP gene designed as OfPGRP8 from the Asian corn borer, Ostrinia furnacalis (Guenée). Its mRNA exhibited the highest abundance in fat body, and its expression level upregulated dramatically after bacterial challenges. Purified recombinant OfPGRP8 exhibited intensive binding capacity to peptidoglycans from Staphylococcus aureus and Micrococcus luteus. Additionally, recombinant OfPGRP8 could inhibit the growth of S. aureus, M. luteus. However, recombinant OfPGRP8 could not cause agglutination of S. aureus, M. luteus or Escherichia coli. Furthermore, we also demonstrated that OfPGRP8 may be involved in modulating the signaling pathway of antimicrobial peptides (AMPs) synthesis. In sum, our results provided evidence that OfPGRP8 dis criminates peptidoglycans from microbes and acts as a PRR to initiate downstream immune signaling pathways.
Low METTL3 level in midgut of the Bombyx mori inhibit the proliferation of nucleopolyhedrovirus
Xue Peng,Jiangtao Zhai,Zhu Juan,Wang Meixian,Zhao Qiaoling,Huang Jinshan,Tang Shunming,Shen Xingjia 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1
N6-methyladeosine (m 6 A) plays an important role in virus infection and replication. Bombyx mori nuclear polyhedrosis is caused by Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Expression levels of m 6 Amodification-related genes after the infection of BmNPV were detected at first. Then, expression levels of BmNPV nucleocapsid protein gene VP39 and envelope fusion protein gene GP64 after knockdown of METTL3in vitro were quantified to identify the effect of m 6 A modification on BmNPV. BmNPV firstly infects the larval midgut in case of oral infection. Subsequently, to clarify the relationship between m 6 A modification and resistance of the silkworm to BmNPV, we detected the expression levels of m 6 A-modification-related genes invivo before and after infection of BmNPV. The results indicated that low METTL3 level hindered the proliferation of BmNPV to some extent, and silkworm strain with low METTL3 level showed stronger resistance against BmNPV. This study will accumulate new experimental data for elucidating the resistance mechanism of silkworm against BmNPV.