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Sequence analysis and expression of the calmodulin gene, MCaM-3, in mulberry (Morus L.)
Rongjun Fang,Dongqing Hu,Yinghua Zhang,Long Li,Weiguo Zhao,Li Liu,Jialin Cheng,Jinliang Qi,Yonghua Yang 한국유전학회 2011 Genes & Genomics Vol.33 No.2
A full-length cDNA sequence encoding CaM from mulberry,which we designated MCaM-3 (GenBank accession No:GQ303247), was cloned based on mulberry expressed sequence tags(ESTs). Sequence analysis showed that MCaM-3is 951 base pairs in length, encoding 149 amino acids with a predicted molecular weight of 16.85 kD and an isoelectric point of 3.95. Online SMART analysis showed that the MCaM-3 protein has four EFh functional domains, which can bind calcium. The expression level of MCaM-3at different developmental stages in mulberry leaves and flowers and in different tissues was investigated. The results showed that MCaM-3 transcripts are most abundantly expressed in mature tissues, such as mature female flowers and climax leaves, and the expression level of the mRNA could be increased significantly under low temperature, drought, and salt stress conditions compared to the normal growth environment. This research will help us understand the resistance mechanism of functional genes in mulberry.
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마동칭(Ma DongQing),윤지영(Yoon Jiyoung),장징위(Zhang JingYu) 한국실내디자인학회 2018 한국실내디자인학회 학술대회논문집 Vol.20 No.3
This study is to explore the characteristics of contemporary Chinese architecture from the aspects of the regionalism of environment, culture and technology. Four cases of contemporary Chinese architecture which won the world-wide famous were selected and analyzed. The results are the followings; First, the regionalism of environment emphasizes the building"s response to the regional environment (topography, climate and regional landscape). Second, the regionalism of culture reflects as the relevance between architecture and community structure, economic patterns, folk customs and other social factors of the region. Third, the regionalism of technology requires the coordination of construction process, structure form, materials and other contents of architecture with the regional environment and culture, and emphasizes the suitability of technology. Environment, culture and technology determine that the regionalism of contemporary Chinese architecture is not purely the meaning at the formal level, but explores the underlying causes.
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Excluding molten fluoride salt from nuclear graphite by SiC/glassy carbon composite coating
Zhao He,Jinliang Song,Pengfei Lian,Dongqing Zhang,Zhanjun Liu 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.5
SiC coating and SiC/glassy carbon composite coating were prepared on IG-110 nuclear graphite (ToyoTanso Co., Ltd., Japan) to strengthen its inertness to molten fluoride salt used in molten salt reactor(MSR). Two kinds of modified graphite were obtained and correspondingly named as IG-110-1 and IG-110-2, which referred to modified IG-110 with a single SiC coating and a SiC/glassy carbon compositecoating, respectively. Both structure and property of modified graphite were carefully researched andcontrasted with virgin IG-110. Results indicated that modified graphite presented better comprehensiveproperties such as more compact structure and higher resistance to molten salt infiltration. With theprotection of coatings, the infiltration amounts of fluoride salt into modified graphite were much lessthan that into virgin IG-110 at the same circumstance. Especially, the infiltration amount of fluoride saltinto IG-110-2 under 5 atm was merely 0.26 wt%, which was much less than that into virgin IG-110 under1.5 atm (13.5 wt%) and the critical index proposed for nuclear graphite used in MSR (0.5 wt%). The SiC/glassy carbon composite coating gave rise to highest resistance to molten salt infiltration into IG-110-2,and thus demonstrated it could be a promising protective coating for nuclear graphite used in MSR.
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In situ observation of mesophase transformation behaviour and mechanistic analysis in β-resin
Liu Ben,Yan Xi,Tao Zechao,Li Xiangfen,Lei Shiwen,Zhang Dongqing,Yang Zonghe,Liu Zhanjun 한국탄소학회 2024 Carbon Letters Vol.34 No.1
β-Resin was extracted by solvent separation of refined coal tar pitch. Several analytical methods revealed that β-resin had a better aromatic plane packing structure and a higher number of carbon residues, making it ideal for mesophase transformation. The mesophase transformation process of β-resin (the formation of liquid-crystalline spheres, the growth of mesophase spheres, and the coalescence and disintegration of mesophase spheres) was observed in situ using a polarizing microscope with a hot stage. Moreover, the mesophase transformation mechanism of β-resin was investigated at each transformation stage. The mesophase content and mesophase transformation kinetics were analyzed based on the area method and quinoline insoluble (QI) substitution method. Both methods revealed changes in the mesophase content of β-resin. However, the test results of the two methods were slightly different at the initial stage of mesophase transformation and tended to be consistent during the later stage.
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