Precisely controlled preparation of uniform nanocrystalline cellulose via microfluidic technology

Shuxiu Wang,Jinsong Zeng,Zheng Cheng,Zhe Yuan,Xiaojun Wang,Bin Wang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.106 No.-

As a new type of biomass-based molecular material, nanocrystalline cellulose (NCC) has become aresearch hotspot in many fields. In present work, a novel microfluidic technology was employed to produceNCC from microcrystalline cellulose (MCC) via sulfuric acid hydrolysis. The microfluidic chip wasdesigned and made with complete load-bearing reaction according to flow characteristics. A closed reactionenvironment and fully automated operations can ensure the safety of experimenters. NCC (MN-60)with high yield and uniformity was obtained via acid hydrolysis in microfluidic system by 60 % sulfuricacid solution at 35 C for 40 min. The characteristics of MN-60 and conventional method NCC (N-60)under the same reaction conditions were compared. It was found that the yield of MN-60 reached48.13%, while it was only 17.30 % in the absence of microfluidic chip. Although the results showed thatMN-60 and N-60 exhibited similar rod-like structures, the size distribution of MN-60 was narrower thanthat of N-60. Furthermore, the width, length, and height of MN-60 were 15 ± 5 nm, 150 ± 75 nm, and5 ± 2 nm, respectively. The main functional groups and crystal forms of MN-60 were similar to MCC,but the crystallization index of MN-60 was higher than MCC. In conclusion, microfluidic technology couldrealize the preparation of high-yield and uniform NCC.

Adaptive diagonal matrix compensation matrix based virtual synchronous generator power decoupling control strategy

Bin Li,Ning Sun,Hao Wang,Dandan Hu,Zhihui Zeng 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.9

This paper discusses the power coupling problem of the traditional virtual synchronous generator (VSG) control strategy in medium and low-voltage microgrids, analyzes the cause of power coupling, and discusses the necessity for decoupling. In addition, a power decoupling control strategy based on an adaptive diagonal matrix compensation matrix is proposed. By compensating for the coupling component, the output power of the VSG is completely decoupled. To cope with the operating characteristics of frequent load switching on the grid side of a microgrid, the proposed strategy tracks variations in the static operating point of the VSG and automatically adjusts the compensation component. Simultaneously, the stability of the VSG is analyzed, using the transient instability criterion of the power system, to ensure decoupling. Finally, the effectiveness of the proposed control strategy is verified by simulation and experimental results.

Study on the Effect of Temperature and Illumination on Omithoctonus huwena's Predation

Wang Zhi,Zeng Bo-Ping,Li Wen-Jian,Wang Wen-Bin,Yan Heng-Mei,Joo-Pil Kim 한국거미연구소 2001 한국거미 Vol.17 No.2

Stable colonization of Akkermansia muciniphila educates host intestinal microecology and immunity to battle against inflammatory intestinal diseases

Wang Bin,Chen Xuheng,Chen Zhiyuan,Xiao Huiwen,Dong Jiali,Li Yuan,Zeng Xiaozhou,Liu Jinjian,Wan Guoyun,Fan Saijun,Cui Ming 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Gut microbial preparations are widely used in treating intestinal diseases but show mixed success. In this study, we found that the therapeutic efficacy of A. muciniphila for dextran sodium sulfate (DSS)-induced colitis as well as intestinal radiation toxicity was ~50%, and mice experiencing a positive prognosis harbored a high frequency of A. muciniphila in the gastrointestinal (GI) tract. Stable GI colonization of A. muciniphila elicited more profound shifts in the gut microbial community structure of hosts. Coexisting with A. muciniphila facilitated proliferation and reprogrammed the gene expression profile of Lactobacillus murinus, a classic probiotic that overtly responded to A. muciniphila addition in a time-dependent manner. Then, a magnetic-drove, mannose-loaded nanophase material was designed and linked to the surface of A. muciniphila. The modified A. muciniphila exhibited enhancements in inflammation targeting and intestinal colonization under an external magnetic field, elevating the positive-response rate and therapeutic efficacy against intestinal diseases. However, the unlinked cocktail containing A. muciniphila and the delivery system only induced negligible improvement of therapeutic efficacy. Importantly, heat-inactivated A. muciniphila lost therapeutic effects on DSS-induced colitis and was even retained in the GI tract for a long time. Further investigations revealed that the modified A. muciniphila was able to drive M2 macrophage polarization by upregulating the protein level of IL-4 at inflammatory loci. Together, our findings demonstrate that stable colonization of live A. muciniphila at lesion sites is essential for its anti-inflammatory function.

An Efficient DHT Routing Protocol with Small-world Features for Structured P2P Network

Bin Zeng,Rui Wang 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.7

Distributed Hash Tables (DHT) provide a fault-tolerant and scalable means to store data blocks in P2P systems. In this thesis, we have proposed an improved version of CAN called “small-world DHT”. The key idea is to link each CAN node to a constant number of long-distance contacts, which are chosen according to the probability density function. This enables our scheme to achieve O(log2n) routing path length with O(1) routing table size per node. We have shown that the hybrid infrastructure of a structured overlay network (CAN) and a random graph (small-world model) not only preserve CAN’s simplicity, but also achieves a resilient and efficient DHT routing algorithm. We have also exploited the LookAhead-GREEDY routing algorithm, whereby each node obtains information about its neighbors’ neighbors by periodically exchanging the routing table entries with its neighbors. The routing algorithm improves the routing efficiency and enhances the network’s resilience to failure. Our scheme requires knowledge of the current network size in order to construct the long-distance links. To estimate the network size, the latter maintains a distributed binary partition tree and measures the size of the sample area by counting the number of leaves in the corresponding branch.

Molecular features and functional studies of transcription factor, Cap ‘n’ collar C, in the brown planthopper, Nilaparvata lugens Stål (Hemiptera Delphacidae)

Zeng Bin,Feng Ze-Rui,Liu Ya-Ting,Chen Fu-Rong,Wang Shuai,Wu Shun-Fan,Yu Zhi-Tao,Gao Cong-Fen 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.4

Cap ‘n’ collar C (CncC), a transcription factor, plays a vital role in the development of insect resistance by regulating the expression of multiple detoxifying genes. Clarifying the molecular characteristics of CncC and its pathway regulating insecticide resistance will aid the development of integrated pest control strategies. Here we cloned and identified the molecular feature of CncC genes from the brown planthopper (BPH), Nilaparvata lugens (Stål), (Hemiptera Delphacidae). The full-length open reading frame of NlCncC was 3015 nucleotides with 1005 amino acids. The deduced amino acid sequence has a high similarity with other insect homologs and contains the characteristic Cnc/bZip domain architecture. Functional studies showed that silencing NlCncC by RNA interference (RNAi) could downregulate the transcript expression of NlCYP6ER1, NlCYP6CW1, and NlCYP6AY1, which increase the susceptibility to imidacloprid in N. lugens. The identification of molecular characteristics of NlCncC and func tional studies in this paper will contribute to designing effective control strategies for N. lugens.

Myeloid-Derived Suppressor Cells Are Associated with Viral Persistence and Downregulation of TCR ζ Chain Expression on CD8⁺ T Cells in Chronic Hepatitis C Patients

Zeng, Qing-Lei,Yang, Bin,Sun, Hong-Qi,Feng, Guo-Hua,Jin, Lei,Zou, Zheng-Sheng,Zhang, Zheng,Zhang, Ji-Yuan,Wang, Fu-Sheng Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.1

Myeloid-derived suppressor cells (MDSCs) play an important role in impairing the function of T cells. We characterized MDSCs in two chronic hepatitis C (CHC) cohorts: a cross-sectional group that included 61 treatment-naive patients with CHC, 14 rapid virologic response (RVR) cases and 22 early virologic response (EVR) cases; and a longitudinal group of 13 cases of RVR and 10 cases of EVR after pegylated-interferon-${\alpha}$/ribavirin treatment for genotype 1b HCV infection. Liver samples from 32 CHC patients and six healthy controls were subjected to immunohistochemical analysis. MDSCs frequency in treatment-naive CHC was significantly higher than in RVR, EVR, or healthy subjects and was positively correlated with HCV RNA. Patients infected with HCV genotype 2a had a significantly higher frequency of MDSCs than those infected with genotype 1b. Decreased T cell receptor (TCR) ${\zeta}$ expression on $CD8^+$ T cells was significantly associated with an increased frequency of MDSCs in treatment-naive CHC patients and was restored by L-arginine treatment in vitro. Increased numbers of liver arginase-$1^+$ cells were closely associated with the histological activity index in CHC. The TCR ${\zeta}$ chain was significantly downregulated on hepatic $CD8^+$ T cells in CHC. During antiviral follow up, MDSCs frequency in peripheral blood mononuclear cells was directly correlated with the HCV RNA load in the plasma and inversely correlated with TCR ${\zeta}$ chain expression in $CD8^+$ T cells in both RVR and EVR cases. Notably, the RVR group had a higher frequency of MDSCs at baseline than the EVR group. Collectively, this study provides evidence that MDSCs might be associated with HCV persistence and downregulation of CD8 ${\zeta}$ chain expression.

Associations between AT-rich Interactive Domain 5B gene Polymorphisms and Risk of Childhood Acute Lymphoblastic Leukemia: a Meta-analysis

Zeng, Hui,Wang, Xue-Bin,Cui, Ning-Hua,Nam, Seungyoon,Zeng, Tuo,Long, Xinghua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Previous genome-wide association studies (GWAS) have implicated several single nucleotide polymorphisms (SNPs) in the AT-rich interactive domain 5B (ARID5B) gene with childhood acute lymphoblastic leukemia (ALL). However, replicated studies reported some inconsistent results in different populations. Using meta-analysis, we here aimed to clarify the nature of the genetic risks contributed by the two polymorphisms (rs10994982, rs7089424) for developing childhood ALL. Through searches of PubMed, EMBASE, and manually searching relevant references, a total of 14 articles with 16 independent studies were included. Odds ratios (ORs) with 95% confidence intervals (95%CI) were calculated to assess the associations. Both SNPs rs10994982 and rs7089424 showed significant associations with childhood ALL risk in all genetic models after Bonferroni correction. Furthermore, subtype analyses of B-lineage ALL provided strong evidence that SNP rs10994982 is highly associated with the risk of developing B-hyperdiploid ALL. These results indicate that SNPs rs10994982 and rs7089424 are indeed significantly associated with increased risk of childhood ALL.

Integrin-linked Kinase Functions as a Tumor Promoter in Bladder Transitional Cell Carcinoma

Wang, De-Lin,Lan, Jian-Hua,Chen, Liang,Huang, Biao,Li, Zeng,Zhao, Xiu-Min,Ma, Qiang,Sheng, Xia,Li, Wen-Bin,Tang, Wei-Xue Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

The aim of this study was to elucidate the role of the integrin-linked kinase (ILK) gene in development of human bladder transitional cell carcinoma (BTCC). Expression of ILK protein and ILK mRNA in 56 cases of human BTCC tissue and in 30 cases of adjacent normal bladder tissue was detected by immunohistochemistry S-P and reverse transcription polymerase chain reaction (RT-PCR), respectively. Four specific miRNA RNAi vectors targeting human ILK were synthesized and transfected into BIU-87 cells by liposome to obtain stable expression cell strains. The influence of ILK on proliferation of BTCC was detected by MTT, FCM on athymic mouse tumorigenesis. The positive rate of ILK protein in BTCC tissue (53.6%) was much higher than adjacent normal bladder tissue (10.0%) (p<0.05). Similarly, expression of ILK mRNA in BTCC tissue ($0.540{\pm}0.083$) was significantly higher than in adjacent normal bladder tissue ($0.492{\pm}0.070$) (p<0.05). MTT showed that the proliferation ability of miRNA-ILK transfected group was clearly decreased (p<0.05), the cell cycle being arrested in G0/G1-S, an tumorigenesis in vivo was also significantly reduced (p<0.05). ILK gene transcription and protein expression may be involved in the development of BTCC, so that ILK might be the new marker for early diagnosis and the new target for gene treatment.

A Mobility Aware and Low Latency Mac Protocol for Mobile Wireless Sensor Network with the Improvement of S_MAC

Bin Zeng,Rui Wang 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.6

There exist a lot of medium access control (MAC) protocols for wireless sensor networks. However, they consider mainly energy efficiency rather than latency which is important in delay sensitive mobile sensor networks. Therefore, a new MAC protocol called MS-MAC is proposed suitable to both stationary and mobile sensor networks. MS-MAC uses an adaptive frame structure improved for well known protocol S-MAC to overcome the performance problems caused by the packets latency in the mobile environment. The selection of monitor nodes and periodic scheduling of synchronization packets are used to predict the speed of mobile nodes, which can save the energy consumption and minimize latencies. Our studies show that under static scenarios, the MS-MAC behavior similar with MS-MAC. However, MS-MAC can increase the network throughput and reduce the latency significantly without more energy loss in comparison with S-MAC in mobile environment.