The role of Jacalin-related lectin gene AOL_s00083g511 in the development and pathogenicity of the nematophagous fungus Arthrobotrys oligospora

Dong Xinyuan,Si Jiali,Zhang Guanghui,Shen Zhen,Zhang Li,Sheng Kangliang,Wang Jingmin,Kong Xiaowei,Zha Xiangdong,Wang Yongzhong 한국미생물학회 2021 The journal of microbiology Vol.59 No.8

Arthrobotrys oligospora is a model species of nematophagous fungi and has great potential for the biological control of nematode diseases. Lectin is a protein that binds to carbohydrates and their complexes with high specificity, which mediates recognition events in various physiological and pathological processes. This study aimed to investigate the role of the Jacalin-related lectin (JRL) gene, AOL_s00083g511, in A. oligospora development. Through a homology recombination approach, we obtained the AOL_s00083g511 knockout mutant strain (Δg511). Next, the biological characteristics of the Δg511 mutant strain, including growth rate, conidia germination rate, adaptation to environmental stresses, and nematocidal activity, were compared with those of the wild-type (WT) strain. The results showed that the JRL gene AOL_ s00083g511 did not affect fungal growth, conidia germination, 3D-trap formation, and the ability of A. oligospora to prey on nematodes significantly. We speculate that this phenomenon may be caused by a loss of the key β1–β2 loops in the AOL_ s00083g511-encoded JRL domain and an intrinsic genetic compensation of AOL_s00083g511 in this fungus. The growth rates of both strains on high salt or surfactant media were similar; however, in the strong oxidation medium, the growth rate of the Δg511 mutant was significantly lower than that of the WT strain, indicating that AOL_s00083g511 might play a role in oxidative stress resistance. These findings provide a basis for further analysis of the related functions of the JRL gene in A. oligospora and their potential roles in the biological control of nematodes in the future.

Study on a Low-temperature Cellulose-degrading Strain: Fermentation Optimization, Straw Degradation, and the Effect of Fermentation Broth on Seed Growth

Xueli Dong,Jing Ji,Songhao Zhang,Danliu Peng,Yurong Wang,Lishuang Zhang,Jiali Li,Gang Wang 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.4

The biodegradation of straw by cellulosedegrading strains plays an important role in the disposal of agricultural waste. In this study, a Penicillium sp. strain JiTF01 that could degrade cellulose was isolated at the low temperature (10oC), and its fermentation conditions were optimized by response surface method. Strain JiTF01 was applied to degrade rice straw at 10oC. It was found that the straw degradation ratio could reach to 46.53% on the 25th day of culture with the enzyme activity of 44.30 U/mL under optimized fermentation conditions. In addition, given that strain JiTF01 showed excellent acid resistance and the cellulase produced by strain JiTF01 exhibited higher enzymatic activity and stability under acidic conditions, the uninterrupted integration process of acid pretreatment and acid fermentation of straw was then performed. The results showed that the degradation ratio of 53.61% was observed after 25 days of the fermentation at pH 3, which increased by 7.08% than that under optimized conditions. Besides, the effect of straw fermentation broth on the growth of rice seed was also investigated, and the 10-5 and 10-6 diluted fermentation broth was found that could significantly promote the growth of rice seedlings and enhance the germination rate of rice seeds under the salt stress of 100 and 200 mmol/L. The conclusions found in this article might have important implications for straw recycling in cold regions.

Genome-wide analysis and environmental response profiling of SOT family genes in rice (Oryza sativa)

Rongjun Chen,Yunyun Jiang,Jiali Dong,Xin Zhang,Hongbo Xiao,Zhengjun Xu,Xiaoling Gao 한국유전학회 2012 Genes & Genomics Vol.34 No.5

Sulphotransferase (SOT) catalyses the transfer of a sulphonate group from 3'-phosphoadenosine 5'-phosphosulphate (PAPS)to an appropriate hydroxyl group of various substrates with the parallel formation of 3'-phosphoadenosine 5'-phosphate (PAP). Although several SOTs have been identified and characterized in mammalian, their role in plant is still unclear. In this study, we report genome-wide comprehensive expression analysis of 35 putative SOT genes in rice. The 35 OsSOTs were tandemly arranged into six clusters. The phylogenetic analysis showed that there were 7 subfamilies of OsSOTs and 11 putatively conserved motifs. Six OsSOTs might be pseudogenes,25 have the two motifs which were involved in PAPS binding regions I and IV. Microarray data indicated that all the OsSOTs were expressed almost at the same level but with different patterns: most OsSOTs were expressed exclusively in stigma and ovary and induced by IAA and BAP, several genes were induced by tZ and DMSO and 11 OsSOTs were response to abiotic stress. Further analysis showed that these 11 genes contained cis-regulatory elements responding to abiotic stresses.

Stable colonization of Akkermansia muciniphila educates host intestinal microecology and immunity to battle against inflammatory intestinal diseases

Wang Bin,Chen Xuheng,Chen Zhiyuan,Xiao Huiwen,Dong Jiali,Li Yuan,Zeng Xiaozhou,Liu Jinjian,Wan Guoyun,Fan Saijun,Cui Ming 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Gut microbial preparations are widely used in treating intestinal diseases but show mixed success. In this study, we found that the therapeutic efficacy of A. muciniphila for dextran sodium sulfate (DSS)-induced colitis as well as intestinal radiation toxicity was ~50%, and mice experiencing a positive prognosis harbored a high frequency of A. muciniphila in the gastrointestinal (GI) tract. Stable GI colonization of A. muciniphila elicited more profound shifts in the gut microbial community structure of hosts. Coexisting with A. muciniphila facilitated proliferation and reprogrammed the gene expression profile of Lactobacillus murinus, a classic probiotic that overtly responded to A. muciniphila addition in a time-dependent manner. Then, a magnetic-drove, mannose-loaded nanophase material was designed and linked to the surface of A. muciniphila. The modified A. muciniphila exhibited enhancements in inflammation targeting and intestinal colonization under an external magnetic field, elevating the positive-response rate and therapeutic efficacy against intestinal diseases. However, the unlinked cocktail containing A. muciniphila and the delivery system only induced negligible improvement of therapeutic efficacy. Importantly, heat-inactivated A. muciniphila lost therapeutic effects on DSS-induced colitis and was even retained in the GI tract for a long time. Further investigations revealed that the modified A. muciniphila was able to drive M2 macrophage polarization by upregulating the protein level of IL-4 at inflammatory loci. Together, our findings demonstrate that stable colonization of live A. muciniphila at lesion sites is essential for its anti-inflammatory function.

Evaluation of Coefficients of Variation for Clinical Chemistry Tests Based on Internal Quality Control Data Across 5,425 Laboratories in China From 2013 to 2022

Wang Wei,Zhang Zhixin,Zhang Chuanbao,Zhao Haijian,Yuan Shuai,Liu Jiali,Dong Na,Wang Zhiguo,Kang Fengfeng 대한진단검사의학회 2024 Annals of Laboratory Medicine Vol.44 No.3

Background: Clinical chemistry tests are most widely used in clinical laboratories, and diverse measurement systems for these analyses are available in China. We evaluated the imprecision of clinical chemistry measurement systems based on internal QC (IQC) data. Methods: IQC data for 27 general chemistry analytes were collected in February each year from 2013 to 2022. Four performance specifications were used to calculate pass rates for CVs of IQC data in 2022. Boxplots were drawn to analyze trends of CVs, and differences in CVs among different groups were assessed using the Mann–Whitney U-test or Kruskal–Wallis test. Results: The number of participating laboratories increased significantly from 1,777 in 2013 to 5,425 in 2022. CVs significantly decreased for all 27 analytes, except creatine kinase and lipase. Triglycerides, total bilirubin, direct bilirubin, iron, and γ-glutamyl transferase achieved pass rates >80% for all goals. Nine analytes with pass rates <80% based on 1/3 allowable total error were further analyzed; the results indicated that closed systems exhibited lower CVs than open systems for all analytes, except total protein. For all nine analytes, differences were significant between tertiary hospitals and non-tertiary hospitals and between accredited and non-accredited laboratories. Conclusions: The CVs of IQC data for clinical chemistry have seen a continuous overall improvement in China. However, there is ample room for imprecision improvement for several analytes, with stricter performance specifications.

An Efficient Sparse Code Fusion Method for Image Enhancement

Jia Li,Yi Dong,Fangyuan Jiao 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.8

Image enhancement can improve the perception of information for human viewers, which is also a basic and pretty significant role in image processing. However, there also exist some limitations in most image enhancement algorithms. In this paper discuss the limitations of existing techniques of image enhancement. In order to solve the limitations well, a novel sparse code fusion (SCF) method is proposed, in which combine piecewise dictionaries strategy. The proposed method firstly is that color space conversion from RGB to Ycbcr color space, secondly enhanced Y component using piecewise sparse code fusion strategy, finally color image reconstruction. Experimental results show that our method can