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Chenjie Meng,Jiaming Zhao,Yuxiang Yin,Jun Luo,Lianying Zhao,Wenbin Jiang,Jianyong Feng 한국섬유공학회 2020 Fibers and polymers Vol.21 No.4
Three-dimensional (3D) printing technology provides an effective alternative for tissue engineering scaffold. Inthis paper, a composite scaffold composed of polylactic acid (PLA) film and 3D printing scaffold was fabricated which couldprovide structural and mechanical support for tissue application. The effects of PLA film/3D printing composite scaffold onscaffold morphology, average diameter, pore diameter, mechanical properties, themal conductivity, electrical conductivity,and surface hydrophilicity were characterized. PLA film/3D printing composite scaffold had larger thickness, bettermechanical and thermal conductivity properties in comparison with pure PLA film and 3D printed scaffold. These resultssuggested that composite scaffold with relatively good overall performance could be a potential candidate for tissueengineering application.
Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies
Liu, Yun,Sun, Xiaoming,Di, Duolong,Feng, Yuxiang,Jin, Fengling Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.7
Many literatures focus on the biological relevance and the identification of biomarkers for disease activity assessment while less attention has been paid to the development of standard procedures for sample preparation and storage based on liquid chromatography technique. The influencing factors including protein precipitation, storage temperature, storage time, and reconstitution by ultra pure water were analyzed employing HPLC-DAD. The effects were investigated from five participants over three months by principal components analysis (PCA) and the values of percent changes (PC). The samples with protein precipitation might slow the rate of bacterial enzymatic conversion. After protein precipitation, the average PC of urine samples ($0.136{\pm}0.013$, n = 5) is relatively less than that of the serum samples ($0.173{\pm}0.026$, n = 5) for three months. Minimal effects on metabolic profiles of serum and urine (PC < 0.15) are reasonable for metabolomic studies after protein precipitation and storage at $-20^{\circ}C$ for two months.
Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies
Yunliu Fan,Xiaoming Sun,Duolong Di,Yuxiang Feng,Fengling Jin 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.7
Many literatures focus on the biological relevance and the identification of biomarkers for disease activity assessment while less attention has been paid to the development of standard procedures for sample preparation and storage based on liquid chromatography technique. The influencing factors including protein precipitation, storage temperature, storage time, and reconstitution by ultra pure water were analyzed employing HPLCDAD. The effects were investigated from five participants over three months by principal components analysis (PCA) and the values of percent changes (PC). The samples with protein precipitation might slow the rate of bacterial enzymatic conversion. After protein precipitation, the average PC of urine samples (0.136 ± 0.013, n = 5) is relatively less than that of the serum samples (0.173 ± 0.026, n = 5) for three months. Minimal effects on metabolic profiles of serum and urine (PC < 0.15) are reasonable for metabolomic studies after protein precipitation and storage at −20 oC for two months.