Residual Characteristics of Selected Pesticides, Cyclaniliprole, Fenazaquin, and Spirodiclofen in Minor Crops

Yun-Seon Kwak,So-Hyun Joo,Seok-Hee Han,Young Soo Keum 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10

Scoparone from Artemisia capillaris Inhibits the Release of Inflammatory Mediators in RAW 264.7 Cells upon Stimulation Cells by Interferon-γ Plus LPS

Seon Il Jang,Young-Jun Kim,Woo-Yiel Lee,Kyung Chell Kwak,Seung Hwa Baek,Gyu Beum Kwak,Young-Gab Yun,Tae-Oh Kwon,Hun Taeg Chung,Kyu-Yun Chai 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.2

Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. In the present study we observed that, scorparone exhibited no cytotoxic effect in unstimulated macrophages, but reduced the release of nitric oxide (NO) and prostaglandin E2 (PGE2) upon stimulation by IFN-γ/LPS or LPS. The inhibitory effects were found to be in conjuction with the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in IFN-γ/LPS stimulated RAW 264.7 cells. Moreover, scoparone also attenuated the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in LPS-stimulated RAW264.7 cells. These results suggest that scoparone decreases the production of the inflammatory mediators such as NO and PGE2 in macrophages by inhibiting iNOS and COX-2 expression.

소면적 재배 농산물 중 Flupyradifurone 및 Oxathiapiprolin의 잔류허용기준 설정을 위한 잔류 분석법 확립

곽윤선 ( Yun-seon Kwak ),한석희 ( Seokhee Han ),송민호 ( Minho Song ),금영수 ( Young-soo Keum ) 한국환경농학회 2019 한국환경농학회 학술대회집 Vol.2019 No.-

농산물 잔류농약에 의한 건강 위해성과 관련하여 2019년 1월부터 Positive List System (PLS)제도를 도입하여 실시하고 있다. 해당 제도의 시행으로 상대적으로 등록 농약이 한정된 소규모 재배 농산물에 대한 합리적이지 않은 농약의 사용으로 부적합 판정을 받는 사례가 발생하여, 이와 같은 소면적 재배 농산물의 농약 잔류허용기준의 설정이 시급하다. 본 연구는 이와 같은 소면적 재배 농산물에 대한 그룹 잔류허용기준 설정을 최종 목적으로 하여 잔류소장, 수준 및 안전사용기준 확보와 관련된 연구이다. 그룹 대표농산물로 엇갈이배추, 시금치, 들깻잎, 미나리 및 쪽파를 그룹 대표 농산물로 선정하였으며, acetylcholine receptor 저해효과를 지닌 살충제 Flupyradifurone 및 난균강 진균성 병원균 살균제 Oxathiapiprolin에 대한 분석법을 개발하고, 작물별 잔류자료를 산출하고자 하였다. 분석법은 식품공전 중 해당 농약의 분석법을 기본으로 하되, 회수율, 분석방해성분 등의 문제점을 확인하고 LC-MS를 활용, 정량적 분석법의 개선을 시도하였으며, 본 화합물과 다수 대사체의 분석을 실시하고 있다. 개별 대표작물에 대하여 산출된 결과를 바탕으로 소면적 재배 농산물의 농약의 안전사용기준(안)및 농약 잔류허용기준설정고시(안)을 마련하고자 한다.

Scoparone from Artemisia capillaris Inhibits the Release of Inflammatory Mediators in RAW 264.7 Cells upon Stimulation Cells by Interferon-${\gamma}$ Plus LPS

Jang Seon Il,Kim Young-Jun,Lee Woo-Yiel,Kwak Kyung Chell,Baek Seung Hwa,Kwak Gyu Beum,Yun Young-Gab,Kwon Tae-Oh,Chung Hun Taeg,Chai Kyu-Yun The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.2

Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. In the present study we observed that, scorparone exhibited no cytotoxic effect in unstimulated macrophages, but reduced the release of nitric oxide (NO) and prostaglandin $E_2\;(PGE_2)$ upon stimulation by IFN-${\gamma}$/LPS or LPS. The inhibitory effects were found to be in conjuction with the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in IFN-${\gamma}$/LPS stimulated RAW 264.7 cells. Moreover, scoparone also attenuated the production of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$ and IL-6 in LPS-stimulated RAW264.7 cells. These results suggest that scoparone decreases the production of the inflammatory mediators such as NO and $PGE_2$ in macrophages by inhibiting iNOS and COX-2 expression.

Chemotherapy Use and Associated Factors among Cancer Patients near the End of Life

Yun, Young Ho,Kwak, Miyoung,Park, Sang Min,Kim, Samyong,Choi, Jong Soo,Lim, Ho-Yeong,Lee, Chang Geol,Choi, Youn Seon,Hong, Young Seon,Kim, Si-Young,Heo, Dae Seog S. Karger AG 2008 Oncology Vol.72 No.3

<P><I>Objectives:</I> We investigated the frequency of chemotherapy use and its associated factors in patients in all age groups in the last year of life. <I>Methods:</I> We identified cancer patients who died in 2004 in any of 17 hospitals. We used demographic and treatment characteristics by computerized hospital information systems and by linking the identification numbers to the 2004 death registry. <I>Results:</I> 48.7% of patients in the last 6 months of life, 43.9% in the last 3 months, and 30.9% in the last month of life received chemotherapy. The frequency of chemotherapy use was lower for older patients. In those ≥65 years old, there was no difference between women and men in the proportion that received chemotherapy. For patients <65 years of age, a larger proportion of women than men received chemotherapy, and chemotherapy use was significantly less frequent for patients with refractory disease than for those with responsive disease. Patients dying at a relatively small hospital without a hospice inpatient unit were significantly more likely to receive chemotherapy. <I>Conclusions:</I> Despite the fact that most cancer patients are resistant to chemotherapy at the end of life, it was administered often to all age groups.</P><P>Copyright © 2007 S. Karger AG, Basel</P>

Intercalation of cobaltocene into WS₂ nanosheets for enhanced catalytic hydrogen evolution reaction

Kwak, In Hye,Abbas, Hafiz Ghulam,Kwon, Ik Seon,Park, Yun Chang,Seo, Jaemin,Cho, Min Kyung,Ahn, Jae-Pyoung,Seo, Hee Won,Park, Jeunghee,Kang, Hong Seok The Royal Society of Chemistry 2019 Journal of Materials Chemistry A Vol.7 No.14

<P>We synthesized cobaltocene (7-20%)-intercalated WS2 nanosheets using a solvothermal process. The intercalation of cobaltocene between the expanded 1T′ phase WS2 layers was confirmed by scanning transmission electron microscopy and electron energy loss spectroscopy. The intercalated complexes exhibited excellent performance for the catalytic hydrogen evolution reaction, with a Tafel slope of 40 mV dec<SUP>−1</SUP> and a current density of 10 mA cm<SUP>−2</SUP> at 0.17 V (<I>vs.</I> RHE). Spin-polarized density functional theory calculations showed that cobaltocene is intercalated with a fivefold symmetry parallel to the WS2 plane, driven by substantial charge transfer. Reaction pathway calculations suggest that the basal S atoms just above the Co atom are the active sites, and the activation barrier of the Heyrovsky reaction determines the catalytic activity.</P>

SWG 조합기술 성능평가를 위한 AHP 기법 적용사례 연구

김윤중 ( Yun Jung Kim ),곽동근 ( Dong Geun Kwak ),김원태 ( Won Tae Kim ),김정주 ( Jeong Joo Kim ),김관엽 ( Kwan Yeop Kim ),김용성 ( Yong Sung Kim ),신흥섭 ( Heung Sup Shin ),김상선 ( Sang Seon Kim ),김채수 ( Chae Soo Kim ) 한국물환경학회(구 한국수질보전학회) 2014 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2014 No.-

Stichoposide D의 백혈병 세포주에서 세라마이드 생성을 통한 세포 사멸 유도 및 항암 작용

Eun-Seon Park(박은선),Seung-Hoon Yun(윤성훈),Sung-Won Shin(신성원),Jong-Young Kwak(곽종영),Joo-In Park(박주인) 한국생명과학회 2012 생명과학회지 Vol.22 No.6

해양 트리테르펜 글리코시드(marine triterpene glycosides)는 해삼(Holothurians)으로부터 분리된 천연물질로서 항 진균작용, 항암작용 및 용혈 작용 등 여러 가지 생물학적 활성들을 가지고 있다고 보고되었다. 또한 이전의 연구 결과 Thelenota anax로부터 분리한 stichoposide C (STC)는 산성 스핑고마이엘리나제와 중성 스핑고마이엘리나제의 활성화에 의한 세라마이드의 생성을 통하여 백혈병 세포주에서 세포사멸을 유도한다는 것을 알 수 있었다. 본 연구에서는 STC와 구조 유사체인 STD가 백혈병 세포에서 세포사멸을 유도하는지와 이에 대한 분자적 기전을 살펴보았다. STC와 STD는 K562 세포와 HL-60 세포에서 농도와 시간 의존적으로 세포 사멸을 일으키고 이러한 세포 사멸은 caspase-8의 활성화, 미토콘드리아 손상, caspase-9의 활성화, 그리고 caspase-3의 활성화에 의해 유도된다. 이러한 결과는 STC와 STD가 외인성 경로와 내인성 경로의 활성화를 통해 세포 사멸을 유도함을 시사한다. 그리고 STC는 산성 SMase와 중성 SMase를 활성화시키고 이 결과로 세라마이드를 생성시킨다. 산성과 중성 SMase의 특이적인 저해제를 이용하여 STC에 의한 세포 사멸이 부분적으로 억제됨을 알 수 있었다. 반면에, STD는 세라마이드 합성 효소의 활성화에 의해서 세라마이드를 생성시킨다. 세라마이드 합성 효소 저해제를 이용하여 STD에 의한 세포 사멸이 부분적으로 억제되는 것을 확인하였다. 더욱이 STC와 STD는 HL-60 세포의 이종이식 종양 모델에서 종양의 성장을 현저하게 억제하였고 세라마이드의 생성도 증가시켰다. 이러한 결과는 STC와 STD가 aglycone에 부착된 당이 다르므로 서로 다른 경로를 통해 세포 사멸과 항암 활성을 유도한다는 것을 암시하였다. 따라서 이러한 결과는 이들의 작용은 aglycone에 부착된 당에 의해 영향을 받을 수 있고 이들은 향후 백혈병의 치료제로 사용될 수 있다는 것을 제시하였다. Marine triterpene glycosides are physiologically active natural compounds isolated from sea cucumbers (holothurians). It was demonstrated that they have a wide range of biological activities, including antifungal, cytotoxic, and antitumor effects. A previous study showed that stichoposide C (STC) isolated from Thelenota anax induces apoptosis through generation of ceramide by activation of acid sphingomyelinase (SMase) and neutral SMase in human leukemia cells. In this study, we investigated whether STD, a structural analog of STC, can induce apoptosis and examined the molecular mechanisms for its activity. It was found that STC and STD induce apoptosis in a dose- and time-dependent manner and lead to the activation of caspase-8, mitochondrial damage, activation of caspase-9, and activation of caspase-3 in K562 and HL-60 cells. STC activates acid SMase and neutral SMase, which results in the generation of ceramide. Specific inhibition of acid SMase or neutral SMase partially blocked STC-induced apoptosis, but not STD-induced apoptosis. In contrast, STD generates ceramide through the activation of ceramide synthase. Specific inhibition of ceramide synthase partially blocked STD-induced apoptosis, but not STC-induced apoptosis. Moreover, STC and STD markedly reduced tumor growth of HL-60 xenograft tumors and increased ceramide generation in vivo. These results indicate that STC and STD can induce apoptosis and have antitumor activity through the different molecular mechanisms, because they have a different sugar residue attached to aglycones. Thus, these results suggest that their actions are affected by a sugar residue attached to aglycones and they can be used as anticancer agents in the treatment of leukemia.

Use of low-dose sulodexide in IgA nephropathy patients on renin-angiotensin system blockades

( Byeong Yun Yang ),( Hee Seon Lee ),( Sang Heon Song ),( Ihm Soo Kwak ),( Soo Bong Lee ),( Dong Won Lee ),( Eun Young Seong ) 대한신장학회 2012 Kidney Research and Clinical Practice Vol.31 No.3

Background: Despite using renin-angiotensin system (RAS) blockades, some of the patients with immunoglobulin A (IgA) nephropathy often had persistent proteinuria of more than 500 mg/d. They need to be managed further by alternative methods to halt the progression of the disease; these methods could also be applied safely over a long period of time. In this context, sulodexide has been studied for the management of diabetic nephropathy. Methods: A retrospective review was carried out involving 20 patients with IgA nephropathy who had been taking sulodexide (50 mg daily) as an add-on therapy together with an optimal dose of RAS blockades during 2008-2009. We evaluated the proteinuria reduction rates and renal function changes. Results: During 11.1±72.7 months of follow-up duration, urinary protein-to- creatinine ratio(UPCR) decreased for 1.57±0.6 to1.17±0.7 g/g (P=0.032). Twenty-five percent of the patients showed a greater than 50% reduction of UPCR, and 40% had a UPCR of less than 1.0 g/g at their final observations. The analysis of the factors contributing to the effect found that a higher pretreatment UPCR showed a significant correlation with the UPCR decrease (r=0.45, P=0.047). Neither the adverse effects nor the renal function impairments were documented during the management. Conclusion: Low-dose sulodexide has an additional modest antiproteinuric effect on IgA nephropathy undergoing RAS blockade therapy.

역량기반 교육과정 개발 및 운용을 위한 교육체계 수립 사례연구

곽윤정(Yun Jung Kwak),장선영(Seon young Jang) 학습자중심교과교육학회 2015 학습자중심교과교육연구 Vol.15 No.12

본 연구는 역량기반 교육과정을 운용하기 위한 교육체계를 수립하기 위한 실재적 인 절차를 구조화하는데 목적이 있으며, 이를 검증하기 위하여 S대학교를 사례로 교육체계 수립하였다. 교육체계를 수립하기 위한 첫 단계로 학교 인재상을 수립하였 다. 이를 위해 창학이념과 학훈을 분석하고, 학습자들의 학교에 기대하는 인식을 조 사하여 분석이 이루어졌다. 두 번째 단계에서는 인재상을 기반으로 대학교에서 양성 하고자 하는 학습자들의 핵심역량을 제시하기 위해 핵심역량 모형이 개발되었다. 이 단계에서는 핵심역량 분석틀을 개발한 후 분석틀을 활용하여 핵심역량 모형이 개발 되었으며, 개발된 1차 모형은 전문가 11인의 검토를 통해 수정·보완되었다. 세 번째 단계에서는 핵심역량모형을 참고하여 학교 및 학과별 교육목적 및 목표를 수립하였 고 해당 대학 내부전문가 20인의 검토를 통해 최종 확정되었다. 마지막 단계에서 이 전 결과물들을 종합하여 교육체계를 수립하였다. 이러한 사례 연구를 바탕으로 역량 기반 교육과정 운용을 위한 교육체계 수립, 교육과정 운용 전략 등을 체계화하는 기 초를 마련해야 할 것이다. The purpose of this study is to structuralize the process for developing educational systems to implement a competency-based curriculum. This process was validated by applying it to the development of educational systems at S university. The first phase of the process was to develop the concept of talent for the university after analyses on the institutional philosophy, school precept, and expectations of learners. The second phase was to develop a core competency model that presented the competencies that were required for the university students. During this phase, a conceptual framework for analyzing core competencies was developed, and this framework was reviewed and modified by 11 experts. The third phase was to establish educational purposes and objectives based on the core competency model. They were also reviewed and decided by 20 experts from the university. Finally, all the outcomes were integrated to develop educational systems. This case study provides foundational knowledge for developing educational systems to implement a competency-based curriculum and for systemizing strategies on managing the educational curriculum.