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Liu, Xiao-Min,An, Jonguk,Han, Hay Ju,Kim, Sun Ho,Lim, Chae Oh,Yun, Dae-Jin,Chung, Woo Sik Springer 2014 Plant cell reports Vol.33 No.12
<P>ZAT11, a Zinc Finger of Arabidopsis Thaliana 11, is a dual-function transcriptional regulator that positively regulates primary root growth but negatively regulates Ni (2+) tolerance. Zinc Finger of Arabidopsis Thaliana 11 (ZAT11) is a C2H2-type zinc finger protein that has been reported to function as an active transcriptional repressor. However, the biological function of ZAT11 remains unknown. Here we show that GFP-tagged ZAT11 is targeted to the nucleus. Analysis of plants expressing ZAT11 promoter-GUS showed that ZAT11 is highly expressed in roots and particularly in root tips. To identify the biological function of ZAT11, we constructed three independent lines of ZAT11 overexpressing transgenic plant (ZAT11 OE). ZAT11 OE enhanced the elongation of primary root but reduced the metal tolerance against nickel ion (Ni(2+)). The reduced Ni(2+) tolerance of ZAT11 OE was correlated with decreased accumulation of Ni(2+) in plants. The decreased accumulation of Ni(2+) in ZAT11 OE was caused by the reduced transcription of a vacuolar Ni(2+) transporter gene. Taken together, our results suggest that ZAT11 is a dual function transcriptional regulator that positively regulates primary root growth but negatively regulates Ni(2+) tolerance.</P>
Liu, Jing-Jing,Zhang, Guo-Chang,Kong, In Iok,Yun, Eun Ju,Zheng, Jia-Qi,Kweon, Dae-Hyuk,Jin, Yong-Su American Society for Microbiology 2018 Applied and environmental microbiology Vol.84 No.10
<P>IMPORTANCE Saccharomyces boulardii is a probiotic yeast strain capable of preventing and treating diarrheal diseases. However, the genetics and metabolism of this yeast are largely unexplored. In particular, molecular mechanisms underlying the inefficient galactose metabolism of S. boulardii remain unknown. Our study reports that a point mutation in PGM2, which codes for phosphoglucomutase, is responsible for inferior galactose utilization by S. boulardii. After correction of the mutated PGM2 via genome editing, the resulting strain was able to use galactose faster than a parental strain. While the PGM2 mutation made the yeast use galactose slowly, investigation of the genomic sequencing data of other S. boulardii strains revealed that the PGM2 mutation is evolutionarily conserved. Interestingly, the PGM2 mutation was beneficial for growth at a higher temperature on glucose. We speculate that the PGM2 mutation was enriched due to selection of S. boulardii in the natural habitat (sugar-rich fruits in tropical areas).</P>
( Ju Hee Kim ),( Woong Sik Nam ),( Doo Hyun Lee ),( Su Youn Lee ),( Hung Chan O ),( Jeong Min Joo ),( Kwang Hyeon Liu ),( Jae Yun Han ),( Sung Hwan Ki ),( Tae Cheon Jeong ),( Tae Ho Lee ),( Sang Kyu L 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-
Cytochrome P450 (cyp) is an important enzyme that can act on xenobiotic substances such as toxic chemicals or drugs. Phenobarbital(PB) has been widely used to induce CYP2B activity to investigate the drug-drug interaction of CTP2B substrate drugs.leelamine is a diterpene compound, and is the current focus of efforts to develop a treatment for diabetes. In this study. we identi-fied the selective and potent inductive effct of leelamine on CYP2B antdoes of 5,10,or 20 mg/kg in male ICR micc for I or 3 days. In liver, the activity of CYP2B significantly increased 3.6-fold after treatment with leelamine,com-pared to vehicle-treated group. Activities of benzyloxy-resorufin O-dealkylase and pentoxyresorufin O-dealkylase significantly increased 6.3-and 5.3-fold, respectively,with a single treatment of 20 mg/kg leelamine for I day. Fur-thermore. immunoblot analysis showed that signifieantly and dose-dependently increased CYP2B10 protein levels in liver.However,PCR results showed that there were no significant changes in the CAR and CYP2B mRNA levels after leelamine treatment. Accordingly, we suggest that leelamine is a novel substitute of PB for the seleetive inudction of CYP2B activity in vivo.
The selective induction of leelamine on hepatic CYP 2B activity in ICR mice
Ju Hee Sim(심주희),Woongsik Nam(남웅식),Hungchan O(오흥찬),Suyoun Lee(이수연),Doohyun Lee(이두현),Kwang-Hyeon Liu(류광현),Taeho Lee(이태호),Jae Yun Han(한재윤),Sung Hwan Ki(기성환),Tae Cheon Jeong(정태천),Sangkyu Lee(이상규) 환경독성보건학회 2014 한국독성학회 심포지움 및 학술발표회 Vol.2014 No.5
Nano pattern transfer on acrylic polymers with UV irradiation for liquid crystal alignment
Lee, Ju Hwan,Jeong, Hae-Chang,Won, Jonghoon,Oh, Byeong-Yun,Kim, Dong Hyun,Lee, Dong Wook,Liu, Yang,Seo, Dae-Shik Elsevier 2019 Polymer Vol.161 No.-
<P><B>Abstract</B></P> <P>Ultra-violet nanoimprint lithography was utilized to transfer a one-dimensional nano pattern onto a hydrophilic ultra-violet cured polymer surface for use in liquid crystal displays. A master mold with the one-dimensional nano pattern was initially constructed using laser interference lithography, which is a fast and maskless process that can be employed over a large area. The UV cured polymer showed a hydrophilic characteristic, and was used as a homogeneous alignment layer. The nano-patterned ultra-violet polymer successfully aligned the liquid crystal molecules without defects. Additionally, the liquid crystal cell was characterized by high thermal stability due to the high thermal endurance of UV cured polymer. Therefore, the proposed ultra-violet nanoimprint lithography technique is beneficial for advanced liquid crystal devices with high brightness and resolution with numerous switching components that require a high thermal cost.</P> <P><B>Highlights</B></P> <P> <UL> <LI> UV imprinting lithography has the advantages of a large processing capacity, cost-effectiveness, and high thermal endurance. </LI> <LI> The master mold created using laser interference lithography has the advantage of maskless and fast patterned processing. </LI> <LI> Hydrophilic UV-cured polymer surface allowed the homogeneous alignment of LCs. </LI> <LI> The topographic grating of a one-dimensional nanopattern to act as a director for unidirectionally aligning the LCs. </LI> <LI> The high thermal endurance of the LC cell based on the UV-cured polymer the nanopattern was achieved up to 180 °C. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
( Xin Liu ),( Ying Dong ),( Jingquan Wang ),( Long Li ),( Zhenmin-zhong ),( Yun-pan Li ),( Shao-jun Chen ),( Yu-cai Fu ),( Wen-can Xu ),( Chi-ju Wei ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.6
Vesicular stomatitis virus G glycoprotein (VSV-G) has been widely used for pseudotyping retroviral, lentiviral, and artificial viral vectors. The objective of this study was to establish a potential approach for large-scale production of VSV-G. To this end, VSV-G was cloned with an N-terminal His-tag into Pichia pastoris expression vector pPIC3.5K. Three clones (Mut<sup>s</sup>) containing the VSV-G expression cassette were identified by PCR. All clones proliferated normally in expansion medium, whereas the proliferation was reduced significantly under induction conditions. VSV-G protein was detected in cell lysates by western blot analysis, and the highest expression level was observed at 96 h post induction. VSV-G could also be obtained from the condition medium of yeast protoplasts. Furthermore, VSV-G could be incorporated into Ad293 cells and was able to induce cell fusion, leading to the transfer of cytoplasmic protein. Finally, VSV-G-mediated DNA transfection was assayed by flow cytometry and luciferase measurement. Incubation of VSV-G lysate with the pGL3-control DNA complex increased the luciferase activity in Ad293 and HeLa cells by about 3-fold. Likewise, incubation of VSV-G lysate with the pCMV-DsRed DNA complex improved the transfection efficiency into Ad293 by 10% and into HeLa cells by about 1-fold. In conclusion, these results demonstrate that VSV-G could be produced from P. pastoris with biofunctionalities, demonstrating that large-scale production of the viral glycoprotein is feasible.
Xu, Yun-ping,Zheng, Zhou-lian,Liu, Chang-jiang,Wu, Kui,Song, Wei-ju Techno-Press 2018 Wind and Structures, An International Journal (WAS Vol.26 No.6
This paper studies the aerodynamic stability of a tensioned, geometrically nonlinear orthotropic membrane structure with hyperbolic paraboloid in sag direction. Considering flow separation, the wind field around membrane structure is simulated as the superposition of a uniform flow and a continuous vortex layer. By the potential flow theory in fluid mechanics and the thin airfoil theory in aerodynamics, aerodynamic pressure acting on membrane surface can be determined. And based on the large amplitude theory of membrane and D'Alembert's principle, interaction governing equations of wind-structure are established. Then, under the circumstance of single-mode response, the Bubnov-Galerkin approximate method is applied to transform the complicated interaction governing equations into a system of second-order nonlinear differential equation with constant coefficients. Through judging the frequency characteristic of the system characteristic equation, the critical velocity of divergence instability is determined. Different parameter analysis shows that the orthotropy, geometrical nonlinearity and scantling of structure is significant for preventing destructive aerodynamic instability in membrane structures. Compared to the model without considering flow separation, it's basically consistent about the divergence instability regularities in the flow separation model.
Moon, Cheil,Liu, Bridget Q.,Kim, So Yeun,Kim, Esther J.,Park, Yun Ju,Yoo, Joo-Yeon,Han, Hyung Soo,Bae, Yong Chul,Ronnett, Gabriele V. Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of neuroscience research Vol.87 No.5
<P>Leukemia inhibitory factor (LIF), a neuropoietic cytokine, has been implicated in the control of neuronal development. We previously reported that LIF plays a critical role in regulating the terminal differentiation of olfactory sensory neurons (OSNs). Here, we demonstrate that LIF plays a complementary role in supporting the survival of immature OSNs. Mature OSNs express LIF, which may be elaborated in a paracrine manner to influence adjacent neurons. LIF null mice display more apoptotic immature neurons than do their wild-type littermates. LIF treatment of dissociated OSNs in vitro significantly reduces the apoptosis of immature OSNs. Double immunocytochemical analysis indicates that the survival of immature OSNs is dependent on the presence of LIF. LIF activates the phosphoinositide 3-kinase (PI3K) pathways and induces the expression of the antiapoptotic molecule Bcl-2 in OSNs, whereas inhibition of the PI3K pathway blocks LIF-dependent OSN survival and Bcl-2 induction. Thus, LIF plays a central role in maintaining the size and integrity of the population of immature neurons within the olfactory epithelium; this population is critical to the rapid recovery of olfactory function after injury. LIF may play a similar role elsewhere in the CNS and thus be important for manipulation of stem cell populations for therapeutic interventions. © 2008 Wiley-Liss, Inc.</P>
TiO₂ Nanotube Photocatalysts Decorated with Perovskite Quantum Dots for CO₂ Conversion
Chen-Chin Lee,Yun-Ju Liu,Hyeonseok Lee 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Nanostructured TiO₂ is considered as a promising photocatalyst for CO₂ conversion. However, even the nanostructured TiO₂ can not overcome its innate limitations such as its wide bandgap. In this work, we demonstrate the synthesis of perovskite quantum dots (PQDs) and its incorporation to TiO₂ nanotubes (TNTs) as light absorber. Two different PQDs based on CH₃NH₃PbBr₃ and CH3NH3PbI3 are prepared and decorated to TNT. The PQD-decorated TNTs are characterized, varying the PQD solution concentration. The PQD-decorated TNTs show the enhanced absorbance through all measured wavelengths and excellent charge tranfer properties. In particular, among the samples, the PQD/TNT photocatalysts prepared with 70-times diluted PQD solution produce the most improved photocurrents under CO₂-saturated electrolyte both for CH₃NH₃PbBr₃ QD/TNT (~50 μA) and CH₃NH₃PbI₃ QD/TNT (~70 μA), suggesting superior photocatalytic capability for CO₂ conversion.